Restriction map for
pFN21ASDA1341
(Linear) (Six-base) MAPSORT of: KIAA1341.seq Check: 8848 from: 1 to: 1800
KIAA1341.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 65 1800
Size: 65 1735
AccI GT'mk_AC
Cuts at: 0 687 1299 1800
Size: 687 612 501
Fragments arranged by size:
687 612 501
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 680 1800
Size: 680 1120
AflIII A'CryG_T
Cuts at: 0 588 1800
Size: 588 1212
ApoI r'AATT_y
Cuts at: 0 261 439 454 618 1071 1624 1800
Size: 261 178 15 164 453 553 176
Fragments arranged by size:
553 453 261 178 176 164 15
AvaI C'yCGr_G
Cuts at: 0 28 1800
Size: 28 1772
BamHI G'GATC_C
Cuts at: 0 525 1800
Size: 525 1275
BanI G'GyrC_C
Cuts at: 0 24 1800
Size: 24 1776
BanII G_rGCy'C
Cuts at: 0 395 1800
Size: 395 1405
BbsI GAAGACnn'nnnn_
Cuts at: 0 510 803 1800
Size: 510 293 997
Fragments arranged by size:
997 510 293
BfrBI ATG'CAT
Cuts at: 0 909 1800
Size: 909 891
BglI GCCn_nnn'nGGC
Cuts at: 0 16 1800
Size: 16 1784
BglII A'GATC_T
Cuts at: 0 351 1800
Size: 351 1449
Bme1580I G_kGCm'C
Cuts at: 0 29 271 829 1800
Size: 29 242 558 971
Fragments arranged by size:
971 558 242 29
BmgBI CAC'GTC
Cuts at: 0 591 1255 1800
Size: 591 664 545
Fragments arranged by size:
664 591 545
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 1430 1800
Size: 293 1137 370
Fragments arranged by size:
1137 370 293
BsaAI yAC'GTr
Cuts at: 0 387 985 1800
Size: 387 598 815
Fragments arranged by size:
815 598 387
BsaBI GATnn'nnATC
Cuts at: 0 1502 1515 1800
Size: 1502 13 285
Fragments arranged by size:
1502 285 13
BsaHI Gr'CG_yC
Cuts at: 0 8 1800
Size: 8 1792
BsaWI w'CCGG_w
Cuts at: 0 1433 1800
Size: 1433 367
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 575 605 1800
Size: 575 30 1195
Fragments arranged by size:
1195 575 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1148 1202 1800
Size: 1148 54 598
Fragments arranged by size:
1148 598 54
BsiHKAI G_wGCw'C
Cuts at: 0 395 1800
Size: 395 1405
Bsp1286I G_dGCh'C
Cuts at: 0 29 271 395 829 1800
Size: 29 242 124 434 971
Fragments arranged by size:
971 434 242 124 29
BspHI T'CATG_A
Cuts at: 0 939 1800
Size: 939 861
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 65 1800
Size: 65 1735
BsrDI GCAATG_nn'
Cuts at: 0 553 1169 1319 1800
Size: 553 616 150 481
Fragments arranged by size:
616 553 481 150
BsrFI r'CCGG_y
Cuts at: 0 682 1800
Size: 682 1118
BstYI r'GATC_y
Cuts at: 0 351 525 1342 1366 1800
Size: 351 174 817 24 434
Fragments arranged by size:
817 434 351 174 24
BtgI C'CryG_G
Cuts at: 0 85 104 1215 1800
Size: 85 19 1111 585
Fragments arranged by size:
1111 585 85 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1237 1800
Size: 1237 563
BtsI GCAGTG_nn'
Cuts at: 0 1314 1800
Size: 1314 486
ClaI AT'CG_AT
Cuts at: 0 1496 1800
Size: 1496 304
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 687 722 1800
Size: 687 35 1078
Fragments arranged by size:
1078 687 35
EaeI y'GGCC_r
Cuts at: 0 1194 1800
Size: 1194 606
EarI CTCTTCn'nnn_
Cuts at: 0 179 938 1800
Size: 179 759 862
Fragments arranged by size:
862 759 179
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 127 1800
Size: 19 108 1673
Fragments arranged by size:
1673 108 19
EcoICRI GAG'CTC
Cuts at: 0 393 1800
Size: 393 1407
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 680 1430 1800
Size: 293 387 750 370
Fragments arranged by size:
750 387 370 293
EcoRI G'AATT_C
Cuts at: 0 261 439 1800
Size: 261 178 1361
Fragments arranged by size:
1361 261 178
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 575 589 607 621 913 945 1800
Size: 575 14 18 14 292 32 855
Fragments arranged by size:
855 575 292 32 18 14 14
MfeI C'AATT_G
Cuts at: 0 848 1800
Size: 848 952
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 715 1800
Size: 715 1085
MslI CAynn'nnrTG
Cuts at: 0 1003 1498 1800
Size: 1003 495 302
Fragments arranged by size:
1003 495 302
MspA1I CmG'CkG
Cuts at: 0 87 106 1723 1800
Size: 87 19 1617 77
Fragments arranged by size:
1617 87 77 19
NdeI CA'TA_TG
Cuts at: 0 324 1800
Size: 324 1476
NsiI A_TGCA'T
Cuts at: 0 911 1800
Size: 911 889
NspI r_CATG'y
Cuts at: 0 913 1800
Size: 913 887
PflMI CCAn_nnn'nTGG
Cuts at: 0 1111 1800
Size: 1111 689
PfoI T'CCnGG_A
Cuts at: 0 579 1800
Size: 579 1221
PmlI CAC'GTG
Cuts at: 0 985 1800
Size: 985 815
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 678 710 1800
Size: 678 32 1090
Fragments arranged by size:
1090 678 32
PstI C_TGCA'G
Cuts at: 0 62 1738 1800
Size: 62 1676 62
Fragments arranged by size:
1676 62 62
PvuII CAG'CTG
Cuts at: 0 1723 1800
Size: 1723 77
SacI G_AGCT'C
Cuts at: 0 395 1800
Size: 395 1405
SacII CC_GC'GG
Cuts at: 0 88 107 1800
Size: 88 19 1693
Fragments arranged by size:
1693 88 19
SexAI A'CCwGG_T
Cuts at: 0 1464 1800
Size: 1464 336
SfcI C'TryA_G
Cuts at: 0 58 212 1734 1800
Size: 58 154 1522 66
Fragments arranged by size:
1522 154 66 58
SmaI CCC'GGG
Cuts at: 0 30 1800
Size: 30 1770
SpeI A'CTAG_T
Cuts at: 0 1228 1800
Size: 1228 572
SspI AAT'ATT
Cuts at: 0 516 1800
Size: 516 1284
Tth111I GACn'n_nGTC
Cuts at: 0 1013 1800
Size: 1013 787
XmaI C'CCGG_G
Cuts at: 0 28 1800
Size: 28 1772
XmnI GAAnn'nnTTC
Cuts at: 0 857 1800
Size: 857 943
Enzymes that do cut:
AarI AccI AcuI AflIII ApoI AvaI BamHI BanI
BanII BbsI BfrBI BglI BglII Bme1580I BmgBI BpmI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BsiHKAI Bsp1286I
BspHI BspMI BsrDI BsrFI BstYI BtgI BtgZI BtsI
ClaI CspCI EaeI EarI EciI EcoICRI Eco57MI EcoRI
Hin4I MfeI MmeI MslI MspA1I NdeI NsiI NspI
PflMI PfoI PmlI PsrI PstI PvuII SacI SacII
SexAI SfcI SmaI SpeI SspI Tth111I XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BbeI BbvCI BcgI BciVI BclI BlpI
BmrI BmtI BplI Bpu10I BpuEI BsaI BseYI BsgI
BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I DraI
DraIII DrdI EagI EcoNI EcoO109I EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MluI MscI NaeI NarI NcoI NgoMIV NheI NotI
NruI PacI PasI PciI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PvuI RsrII SalI SanDI SapI
SbfI ScaI SfiI SfoI SgfI SgrAI SmlI SnaBI
SphI SrfI StuI StyI SwaI TaqII TatI TstI
XbaI XcmI XhoI ZraI