Restriction map for
pFN21ASDA1337
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# NO CUT ENZYMES ( 64 enzymes) #
##################################
AclI AcsI AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AvrII BclI
BlnI BsaBI BsiEI BsmI BspDI
BspLU11I Bst1107I BstBI BstZ17I ClaI
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoRV FbaI
FseI HindIII HpaI MamI MfeI
MluI MunI NdeI NotI NruI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SfuI SgrAI SnaBI SphI SspI
SwaI XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 2406 GACGTC GACGT^C
Acc65I 692 GGTACC G^GTACC
AccIII 2402 TCCGGA T^CCGGA
AflII 376 CTTAAG C^TTAAG
AocI 2458 CCTGAGG CC^T[ACGT]AGG
Asp718I 692 GGTACC G^GTACC
AspI 1594 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 391 GGATCC G^GATCC
BbvCI 3636 CCTCAGC CCTCAGC(-5/-2)
BcgI 3167 GCAAGGCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 376 CTTAAG C^TTAAG
BglII 1052 AGATCT A^GATCT
BsaWI 2402 TCCGGA [AT]^CCGG[AT]
BseAI 2402 TCCGGA T^CCGGA
BsiWI 3841 CGTACG C^GTACG
Bsp1407I 2492 TGTACA T^GTACA
BspEI 2402 TCCGGA T^CCGGA
BspHI 1016 TCATGA T^CATGA
BsrDI 1416 CATTGC GCAATG(2/0)
BsrGI 2492 TGTACA T^GTACA
Bsu36I 2458 CCTGAGG CC^T[ACGT]AGG
DraIII 955 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3079 GACAACCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2458 CCTGAGG CC^T[ACGT]AGG
EcoT22I 2847 ATGCAT ATGCA^T
KpnI 692 GGTACC GGTAC^C
MroI 2402 TCCGGA T^CCGGA
MseI 377 TTAA T^TAA
NheI 2449 GCTAGC G^CTAGC
NsiI 2847 ATGCAT ATGCA^T
NspI 1385 ACATGC [GA]CATG^[CT]
PflFI 1594 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 1016 TCATGA T^CATGA
SalI 3372 GTCGAC G^TCGAC
SbfI 2469 CCTGCAGG CCTGCA^GG
ScaI 1538 AGTACT AGT^ACT
SexAI 3761 ACCTGGT A^CC[AT]GGT
SpeI 3009 ACTAGT A^CTAGT
Sse8387I 2469 CCTGCAGG CCTGCA^GG
SspBI 2492 TGTACA T^GTACA
TfiI 4149 GATTC G^A[AT]TC
Tru9I 377 TTAA T^TAA
Tth111I 1594 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflIII 1937 ACGTGT A^C[GA][CT]GT
2057 ACGTGT
BbeI 754 GGCGCC GGCGC^C
4032 GGCGCC
BbrPI 1519 CACGTG CAC^GTG
3862 CACGTG
BciVI 3015 GGATAC GTATCC(6/5)
1532 GTATCC
BlpI 675 GCTGAGC GC^T[ACGT]AGC
2871 GCTTAGC
Bpu1102I 675 GCTGAGC GC^T[ACGT]AGC
2871 GCTTAGC
BssHII 777 GCGCGC G^CGCGC
841 GCGCGC
BstEII 1278 GGTCACC G^GT[ACGT]ACC
3666 GGTGACC
BstPI 1278 GGTCACC G^GT[ACGT]ACC
3666 GGTGACC
CelII 675 GCTGAGC GC^T[ACGT]AGC
2871 GCTTAGC
EcoO65I 1278 GGTCACC G^GT[ACGT]ACC
3666 GGTGACC
HincII 3058 GTCAAC GT[CT]^[GA]AC
3372 GTCGAC
HindII 3058 GTCAAC GT[CT]^[GA]AC
3372 GTCGAC
KasI 754 GGCGCC G^GCGCC
4032 GGCGCC
NarI 754 GGCGCC GG^CGCC
4032 GGCGCC
PflMI 1074 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1536 CCAGTACTTGG
PmaCI 1519 CACGTG CAC^GTG
3862 CACGTG
PmlI 1519 CACGTG CAC^GTG
3862 CACGTG
SfiI 1290 GGCCATGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3585 GGCCGCGGTGGCC
SfoI 754 GGCGCC GGC^GCC
4032 GGCGCC
SmaI 3099 CCCGGG CCC^GGG
3300 CCCGGG
StuI 341 AGGCCT AGG^CCT
3455 AGGCCT
Tsp509I 1900 AATT ^AATT
3144 AATT
Van91I 1074 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1536 CCAGTACTTGG
XmaCI 3099 CCCGGG C^CCGGG
3300 CCCGGG
XmaI 3099 CCCGGG C^CCGGG
3300 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII AviII BalI BanI
BanII BbsI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI FspI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI SacI SacII
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TseI
Tsp45I TspRI TthHB8I XcmI XhoII