(Circular) (Six-base) MAPSORT of: pFN21ASDA1337.seq Check: 80 from: 1 to: 8905 pFN21ASDA1337 8905 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5086 5086 Size: 8905 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4433 6715 278 Size: 53 83 186 936 2897 2282 2468 Fragments arranged by size: 2897 2468 2282 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3332 3453 4554 4688 5136 5396 6248 1937 Size: 1395 121 1101 134 448 260 852 4594 Fragments arranged by size: 4594 1395 1101 852 448 260 134 121 Acc65I G'GTAC_C Cuts at: 2715 6226 2715 Size: 3511 5394 AclI AA'CG_TT Cuts at: 1563 7032 7405 1563 Size: 5469 373 3063 Fragments arranged by size: 5469 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2450 3212 4765 5232 6968 8352 8620 1632 Size: 818 762 1553 467 1736 1384 268 1917 Fragments arranged by size: 1917 1736 1553 1384 818 762 467 268 AflII C'TTAA_G Cuts at: 820 1017 2399 820 Size: 197 1382 7326 Fragments arranged by size: 7326 1382 197 AflIII A'CryG_T Cuts at: 1203 3960 4080 7804 8781 1203 Size: 2757 120 3724 977 1327 Fragments arranged by size: 3724 2757 1327 977 120 AgeI A'CCGG_T Cuts at: 7736 7736 Size: 8905 AhdI GACnn_n'nnGTC Cuts at: 7634 7634 Size: 8905 AleI CACnn'nnGTG Cuts at: 1166 3308 6002 1166 Size: 2142 2694 4069 Fragments arranged by size: 4069 2694 2142 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4748 5318 6056 1184 Size: 3564 570 738 4033 Fragments arranged by size: 4033 3564 738 570 AlwNI CAG_nnn'CTG Cuts at: 2497 2698 3103 3435 4168 4327 5242 5473 6648 Size: 201 405 332 733 159 915 231 1175 Cuts at: 6648 8220 2497 Size: 1572 3182 Fragments arranged by size: 3182 1572 1175 915 733 405 332 231 201 159 ApaI G_GGCC'C Cuts at: 2116 2130 4007 4291 5236 6127 2116 Size: 14 1877 284 945 891 4894 Fragments arranged by size: 4894 1877 945 891 284 14 ApaLI G'TGCA_C Cuts at: 6962 8118 6962 Size: 1156 7749 ApoI r'AATT_y Cuts at: 1493 6214 6440 6592 8701 1493 Size: 4721 226 152 2109 1697 Fragments arranged by size: 4721 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 7459 160 Size: 872 6427 1606 Fragments arranged by size: 6427 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8905 AvaI C'yCGr_G Cuts at: 1870 1943 2529 3974 4103 5122 5306 5323 5486 Size: 73 586 1445 129 1019 184 17 163 Cuts at: 5486 6230 1870 Size: 744 4545 Fragments arranged by size: 4545 1445 1019 744 586 184 163 129 73 17 BamHI G'GATC_C Cuts at: 2414 6235 6602 2414 Size: 3821 367 4717 Fragments arranged by size: 4717 3821 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2715 2777 3883 4508 4621 Size: 325 217 627 928 62 1106 625 113 Cuts at: 4621 4876 5172 6055 6104 6137 6226 7681 618 Size: 255 296 883 49 33 89 1455 1842 Fragments arranged by size: 1842 1455 1106 928 883 627 625 325 296 255 217 113 89 62 49 33 BanII G_rGCy'C Cuts at: 1391 2116 2130 2536 2853 3008 3122 3200 3899 Size: 725 14 406 317 155 114 78 699 Cuts at: 3899 4007 4291 4718 5236 5313 5495 6127 6224 Size: 108 284 427 518 77 182 632 97 Cuts at: 6224 1391 Size: 4072 Fragments arranged by size: 4072 725 699 632 518 427 406 317 284 182 155 114 108 97 78 77 14 BbeI G_GCGC'C Cuts at: 2781 6059 2781 Size: 3278 5627 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2685 3672 3754 3941 5056 928 Size: 971 786 987 82 187 1115 4777 Fragments arranged by size: 4777 1115 987 971 786 187 82 BbvCI CC'TCA_GC Cuts at: 5660 5660 Size: 8905 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5179 5213 7096 7130 1903 Size: 34 3242 34 1883 34 3678 Fragments arranged by size: 3678 3242 1883 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3566 5032 6797 8007 3566 Size: 1466 1765 1210 4464 Fragments arranged by size: 4464 1765 1466 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 8905 BfrBI ATG'CAT Cuts at: 4872 4872 Size: 8905 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3320 4286 5615 Size: 107 122 71 631 605 1648 966 1329 Cuts at: 5615 7516 136 Size: 1901 1525 Fragments arranged by size: 1901 1648 1525 1329 966 631 605 122 107 71 BglII A'GATC_T Cuts at: 3075 8900 3075 Size: 5825 3080 BlpI GC'TnA_GC Cuts at: 2699 4895 6322 2699 Size: 2196 1427 5282 Fragments arranged by size: 5282 2196 1427 Bme1580I G_kGCm'C Cuts at: 1790 2116 2130 2770 4007 4291 4626 4956 5175 Size: 326 14 640 1237 284 335 330 219 Cuts at: 5175 5236 5914 5940 6127 6966 8122 1790 Size: 61 678 26 187 839 1156 2573 Fragments arranged by size: 2573 1237 1156 839 678 640 335 330 326 284 219 187 61 26 14 BmgBI CAC'GTC Cuts at: 1318 3961 4081 5110 1318 Size: 2643 120 1029 5113 Fragments arranged by size: 5113 2643 1029 120 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2401 4189 4831 Size: 416 53 83 399 24 984 1788 642 Cuts at: 4831 5230 6636 7594 442 Size: 399 1406 958 1753 Fragments arranged by size: 1788 1753 1406 984 958 642 416 399 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4476 1056 Size: 3420 5485 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3193 4381 4642 3193 Size: 1188 261 7456 Fragments arranged by size: 7456 1188 261 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2224 2672 3146 3889 3911 4061 4088 4457 Size: 985 448 474 743 22 150 27 369 Cuts at: 4457 4688 4885 5447 5725 5829 5890 5891 7565 Size: 231 197 562 278 104 61 1 1674 Cuts at: 7565 1239 Size: 2579 Fragments arranged by size: 2579 1674 985 743 562 474 448 369 278 231 197 150 104 61 27 22 1 Bpu10I CC'TnA_GC Cuts at: 4349 4456 5660 4349 Size: 107 1204 7594 Fragments arranged by size: 7594 1204 107 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2972 4322 6077 7030 7895 8193 2972 Size: 1350 1755 953 865 298 3684 Fragments arranged by size: 3684 1755 1350 953 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2633 3515 5112 7568 882 Size: 617 1134 882 1597 2456 2219 Fragments arranged by size: 2456 2219 1597 1134 882 617 BsaAI yAC'GTr Cuts at: 493 1204 3310 3544 4792 5403 5887 6202 6696 Size: 711 2106 234 1248 611 484 315 494 Cuts at: 6696 493 Size: 2702 Fragments arranged by size: 2702 2106 1248 711 611 494 484 315 234 BsaBI GATnn'nnATC Cuts at: 8899 8899 Size: 8905 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2778 4430 5079 5352 Size: 53 83 186 936 1245 1652 649 273 Cuts at: 5352 5841 6056 6712 7094 7729 275 Size: 489 215 656 382 635 1451 Fragments arranged by size: 1652 1451 1245 936 656 649 635 489 382 273 215 186 83 53 BsaWI w'CCGG_w Cuts at: 4425 7338 7736 8010 8157 8568 4425 Size: 2913 398 274 147 411 4762 Fragments arranged by size: 4762 2913 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2438 2468 3749 3779 4547 Size: 30 165 30 1035 30 1281 30 768 Cuts at: 4547 4577 5246 5276 1178 Size: 30 669 30 4807 Fragments arranged by size: 4807 1281 1035 768 669 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2081 2084 2093 5254 5648 5752 1186 Size: 895 3 9 3161 394 104 4339 Fragments arranged by size: 4339 3161 895 394 104 9 3 BseYI C'CCAG_C Cuts at: 2188 2217 2243 2723 2787 3001 3029 3264 3775 Size: 29 26 480 64 214 28 235 511 Cuts at: 3775 4072 4209 5448 5712 6043 6059 6133 8108 Size: 297 137 1239 264 331 16 74 1975 Cuts at: 8108 8604 2188 Size: 496 2489 Fragments arranged by size: 2489 1975 1239 511 496 480 331 297 264 235 214 137 74 64 29 28 26 16 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3929 4157 4175 5390 5582 1163 Size: 573 2193 228 18 1215 192 4486 Fragments arranged by size: 4486 2193 1215 573 228 192 18 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6355 7116 8144 1540 Size: 275 186 4354 761 1028 2301 Fragments arranged by size: 4354 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2256 2853 2988 3200 3232 3899 5529 5658 6153 Size: 597 135 212 32 667 1630 129 495 Cuts at: 6153 6966 7051 8122 2256 Size: 813 85 1071 3039 Fragments arranged by size: 3039 1630 1071 813 667 597 495 212 135 129 85 32 BsiWI C'GTAC_G Cuts at: 5864 5864 Size: 8905 BsmI GAATG_Cn' Cuts at: 6416 6509 6416 Size: 93 8812 BsmBI CGTCTCn'nnnn_ Cuts at: 2453 3951 4071 4237 4970 5908 2453 Size: 1498 120 166 733 938 5450 Fragments arranged by size: 5450 1498 938 733 166 120 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2116 2130 2256 2536 2770 2853 2988 Size: 399 326 14 126 280 234 83 135 Cuts at: 2988 3008 3122 3200 3232 3899 4007 4291 4626 Size: 20 114 78 32 667 108 284 335 Cuts at: 4626 4718 4956 5175 5236 5313 5495 5529 5658 Size: 92 238 219 61 77 182 34 129 Cuts at: 5658 5914 5940 6127 6153 6224 6966 7051 8122 Size: 256 26 187 26 71 742 85 1071 Cuts at: 8122 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 667 399 335 326 284 280 256 238 234 219 187 182 135 129 126 114 108 92 85 83 78 77 71 61 34 32 26 26 20 14 BspEI T'CCGG_A Cuts at: 4425 8568 4425 Size: 4143 4762 BspHI T'CATG_A Cuts at: 3039 6794 8524 3039 Size: 3755 1730 3420 Fragments arranged by size: 3755 3420 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3970 5086 5325 5451 5528 5808 5826 6260 Size: 3126 1116 239 126 77 280 18 434 Cuts at: 6260 844 Size: 3489 Fragments arranged by size: 3489 3126 1116 434 280 239 126 77 18 BsrBI CCG'CTC Cuts at: 1394 1823 2514 2622 5709 6162 6792 1394 Size: 429 691 108 3087 453 630 3507 Fragments arranged by size: 3507 3087 691 630 453 429 108 BsrDI GCAATG_nn' Cuts at: 65 1248 3438 7400 7574 65 Size: 1183 2190 3962 174 1396 Fragments arranged by size: 3962 2190 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2430 4266 4476 5017 7549 7736 1700 Size: 110 620 1836 210 541 2532 187 2869 Fragments arranged by size: 2869 2532 1836 620 541 210 187 110 BsrGI T'GTAC_A Cuts at: 96 4515 96 Size: 4419 4486 BssHII G'CGCG_C Cuts at: 1925 2800 2864 1925 Size: 875 64 7966 Fragments arranged by size: 7966 875 64 BssSI C'ACGA_G Cuts at: 2360 2978 6146 6965 7977 2360 Size: 618 3168 819 1012 3288 Fragments arranged by size: 3288 3168 1012 819 618 BstAPI GCAn_nnn'nTGC Cuts at: 794 2497 3435 4168 4368 5281 5473 5482 5679 Size: 1703 938 733 200 913 192 9 197 Cuts at: 5679 794 Size: 4020 Fragments arranged by size: 4020 1703 938 913 733 200 197 192 9 BstBI TT'CG_AA Cuts at: 2007 7709 2007 Size: 5702 3203 BstEII G'GTnAC_C Cuts at: 1184 3301 5689 1184 Size: 2117 2388 4400 Fragments arranged by size: 4400 2388 2117 BstXI CCAn_nnnn'nTGG Cuts at: 3661 3791 5114 3661 Size: 130 1323 7452 Fragments arranged by size: 7452 1323 130 BstYI r'GATC_y Cuts at: 1972 2349 2414 2656 2992 3075 3216 3771 5376 Size: 377 65 242 336 83 141 555 1605 Cuts at: 5376 6235 6602 6987 7004 8456 8542 8554 8900 Size: 859 367 385 17 1452 86 12 346 Cuts at: 8900 1972 Size: 1977 Fragments arranged by size: 1977 1605 1452 859 555 385 377 367 346 336 242 141 86 83 65 17 12 Bsu36I CC'TnA_GG Cuts at: 4482 4482 Size: 8905 BtgI C'CryG_G Cuts at: 513 1065 2022 2277 2550 2592 4138 4858 5049 Size: 552 957 255 273 42 1546 720 191 Cuts at: 5049 5338 5382 5610 5766 5919 513 Size: 289 44 228 156 153 3499 Fragments arranged by size: 3499 1546 957 720 552 289 273 255 228 191 156 153 44 42 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5182 5911 5941 487 Size: 683 56 3956 729 30 3451 Fragments arranged by size: 3956 3451 729 683 56 30 BtsI GCAGTG_nn' Cuts at: 3217 5352 5378 6421 7215 7235 3217 Size: 2135 26 1043 794 20 4887 Fragments arranged by size: 4887 2135 1043 794 26 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8905 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3905 3940 529 Size: 35 3341 35 5494 Fragments arranged by size: 5494 3341 35 35 DraI TTT'AAA Cuts at: 6209 6209 Size: 8905 DraIII CAC_nnn'GTG Cuts at: 2983 2983 Size: 8905 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5108 7912 809 Size: 507 3792 2804 1802 Fragments arranged by size: 3792 2804 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2442 2751 3132 Size: 54 1425 50 275 18 612 309 381 Cuts at: 3132 3312 3321 3585 3831 4141 4794 5027 5607 Size: 180 9 264 246 310 653 233 580 Cuts at: 5607 5616 5895 6352 7241 8870 8 Size: 9 279 457 889 1629 43 Fragments arranged by size: 1629 1425 889 653 612 580 457 381 310 309 279 275 264 246 233 180 54 50 43 18 9 9 EagI C'GGCC_G Cuts at: 1537 1812 6352 1537 Size: 275 4540 4090 Fragments arranged by size: 4540 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2261 3188 3536 4047 4304 4439 4526 5162 Size: 908 927 348 511 257 135 87 636 Cuts at: 5162 5948 6835 1353 Size: 786 887 3423 Fragments arranged by size: 3423 927 908 887 786 636 511 348 257 135 87 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2440 3122 4262 7492 7866 8012 Size: 1064 138 851 682 1140 3230 374 146 Cuts at: 8012 387 Size: 1280 Fragments arranged by size: 3230 1280 1140 1064 851 682 374 146 138 EcoICRI GAG'CTC Cuts at: 2851 3198 3897 2851 Size: 347 699 7859 Fragments arranged by size: 7859 699 347 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2224 2450 2672 3146 3212 3889 3911 Size: 393 592 226 222 474 66 677 22 Cuts at: 3911 4061 4088 4457 4688 4765 4885 5232 5447 Size: 150 27 369 231 77 120 347 215 Cuts at: 5447 5725 5829 5890 5891 6968 7565 8352 8620 Size: 278 104 61 1 1077 597 787 268 Cuts at: 8620 1239 Size: 1524 Fragments arranged by size: 1524 1077 787 677 597 592 474 393 369 347 278 268 231 226 222 215 150 120 104 77 66 61 27 22 1 EcoNI CCTnn'n_nnAGG Cuts at: 2139 4920 5159 5189 5789 2139 Size: 2781 239 30 600 5255 Fragments arranged by size: 5255 2781 600 239 30 EcoO109I rG'GnC_Cy Cuts at: 2112 2126 2127 2573 3100 3172 4003 4004 4183 Size: 14 1 446 527 72 831 1 179 Cuts at: 4183 4288 4462 5232 6097 6123 6124 6189 2112 Size: 105 174 770 865 26 1 65 4828 Fragments arranged by size: 4828 865 831 770 527 446 179 174 105 72 65 26 14 1 1 1 EcoRI G'AATT_C Cuts at: 6214 6592 6214 Size: 378 8527 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3733 4795 4846 4870 3733 Size: 1062 51 24 7768 Fragments arranged by size: 7768 1062 51 24 FspI TGC'GCA Cuts at: 3209 3389 3680 7411 3209 Size: 180 291 3731 4703 Fragments arranged by size: 4703 3731 291 180 HaeII r_GCGC'y Cuts at: 1678 2399 2473 2781 2905 4356 4649 5571 5857 Size: 721 74 308 124 1451 293 922 286 Cuts at: 5857 6059 8052 1678 Size: 202 1993 2531 Fragments arranged by size: 2531 1993 1451 922 721 308 293 286 202 124 74 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2438 2470 4046 4078 7546 Size: 32 135 32 866 32 1576 32 3468 Cuts at: 7546 7578 7620 7652 1373 Size: 32 42 32 2626 Fragments arranged by size: 3468 2626 1576 866 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5083 5397 6249 6495 669 Size: 988 281 3145 314 852 246 3079 Fragments arranged by size: 3145 3079 988 852 314 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8905 HpaI GTT'AAC Cuts at: 6495 6495 Size: 8905 KasI G'GCGC_C Cuts at: 2777 6055 2777 Size: 3278 5627 KpnI G_GTAC'C Cuts at: 2719 6230 2719 Size: 3511 5394 MfeI C'AATT_G Cuts at: 6504 6504 Size: 8905 MluI A'CGCG_T Cuts at: 8781 8781 Size: 8905 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3964 4149 8019 8203 1305 Size: 2659 185 3870 184 2007 Fragments arranged by size: 3870 2659 2007 185 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3134 3314 3323 4796 5897 Size: 54 1425 343 1302 180 9 1473 1101 Cuts at: 5897 10 Size: 3018 Fragments arranged by size: 3018 1473 1425 1302 1101 343 180 54 9 MslI CAynn'nnrTG Cuts at: 518 1166 2276 3308 3611 4322 5603 6002 6863 Size: 648 1110 1032 303 711 1281 399 861 Cuts at: 6863 7222 7381 518 Size: 359 159 2042 Fragments arranged by size: 2042 1281 1110 1032 861 711 648 399 359 303 159 MspA1I CmG'CkG Cuts at: 1606 2317 2488 2497 2552 2594 2695 3001 3264 Size: 711 171 9 55 42 101 306 263 Cuts at: 3264 3435 3516 3676 3912 4140 4159 4209 4550 Size: 171 81 160 236 228 19 50 341 Cuts at: 4550 5332 5473 5612 6321 6998 8146 8391 1606 Size: 782 141 139 709 677 1148 245 2120 Fragments arranged by size: 2120 1148 782 711 709 677 341 306 263 245 236 228 171 171 160 141 139 101 81 55 50 42 19 9 NaeI GCC'GGC Cuts at: 4268 4478 5019 4268 Size: 210 541 8154 Fragments arranged by size: 8154 541 210 NarI GG'CG_CC Cuts at: 2778 6056 2778 Size: 3278 5627 NcoI C'CATG_G Cuts at: 513 1065 2022 2277 5049 5382 513 Size: 552 957 255 2772 333 4036 Fragments arranged by size: 4036 2772 957 552 333 255 NdeI CA'TA_TG Cuts at: 387 387 Size: 8905 NgoMIV G'CCGG_C Cuts at: 4266 4476 5017 4266 Size: 210 541 8154 Fragments arranged by size: 8154 541 210 NheI G'CTAG_C Cuts at: 1052 4472 1052 Size: 3420 5485 NotI GC'GGCC_GC Cuts at: 6352 6352 Size: 8905 NsiI A_TGCA'T Cuts at: 4874 4874 Size: 8905 NspI r_CATG'y Cuts at: 1132 1229 3412 6263 7808 1132 Size: 97 2183 2851 1545 2229 Fragments arranged by size: 2851 2229 2183 1545 97 PasI CC'CwG_GG Cuts at: 1348 2212 4000 4467 6100 6127 1348 Size: 864 1788 467 1633 27 4126 Fragments arranged by size: 4126 1788 1633 864 467 27 PciI A'CATG_T Cuts at: 7804 7804 Size: 8905 PflMI CCAn_nnn'nTGG Cuts at: 1108 3103 3565 1108 Size: 1995 462 6448 Fragments arranged by size: 6448 1995 462 PfoI T'CCnGG_A Cuts at: 2955 4392 2955 Size: 1437 7468 PmeI GTTT'AAAC Cuts at: 6209 6209 Size: 8905 PmlI CAC'GTG Cuts at: 3544 5887 3544 Size: 2343 6562 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6974 7006 8487 8519 6974 Size: 32 1481 32 7360 Fragments arranged by size: 7360 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3100 4183 4462 3100 Size: 1083 279 7543 Fragments arranged by size: 7543 1083 279 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8905 PsiI TTA'TAA Cuts at: 6475 6475 Size: 8905 PspOMI G'GGCC_C Cuts at: 2112 2126 4003 4287 5232 6123 2112 Size: 14 1877 284 945 891 4894 Fragments arranged by size: 4894 1877 945 891 284 14 PstI C_TGCA'G Cuts at: 830 2052 2270 2493 3184 3706 3856 4116 4164 Size: 1222 218 223 691 522 150 260 48 Cuts at: 4164 4416 4497 5277 5478 6159 6257 830 Size: 252 81 780 201 681 98 3478 Fragments arranged by size: 3478 1222 780 691 681 522 260 252 223 218 201 150 98 81 48 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8905 PvuII CAG'CTG Cuts at: 2488 2497 3435 3516 3676 3912 4159 5332 5473 Size: 9 938 81 160 236 247 1173 141 Cuts at: 5473 2488 Size: 5920 Fragments arranged by size: 5920 1173 938 247 236 160 141 81 9 SacI G_AGCT'C Cuts at: 2853 3200 3899 2853 Size: 347 699 7859 Fragments arranged by size: 7859 699 347 SacII CC_GC'GG Cuts at: 2553 2595 4141 5613 2553 Size: 42 1546 1472 5845 Fragments arranged by size: 5845 1546 1472 42 SalI G'TCGA_C Cuts at: 1936 5395 6247 1936 Size: 3459 852 4594 Fragments arranged by size: 4594 3459 852 SanDI GG'GwC_CC Cuts at: 4462 4462 Size: 8905 SapI GCTCTTCn'nnn_ Cuts at: 2261 3188 4047 2261 Size: 927 859 7119 Fragments arranged by size: 7119 927 859 SbfI CC_TGCA'GG Cuts at: 4497 6257 4497 Size: 1760 7145 ScaI AGT'ACT Cuts at: 3563 7153 8865 3563 Size: 3590 1712 3603 Fragments arranged by size: 3603 3590 1712 SexAI A'CCwGG_T Cuts at: 5784 5784 Size: 8905 SfcI C'TryA_G Cuts at: 826 1045 2048 2266 2489 3180 3702 3852 4112 Size: 219 1003 218 223 691 522 150 260 Cuts at: 4112 4160 4412 4493 4555 4689 5254 5273 5474 Size: 48 252 81 62 134 565 19 201 Cuts at: 5474 6155 6253 7388 8069 8260 826 Size: 681 98 1135 681 191 1471 Fragments arranged by size: 1471 1135 1003 691 681 681 565 522 260 252 223 219 218 201 191 150 134 98 81 62 48 19 SfiI GGCCn_nnn'nGGCC Cuts at: 3320 5615 3320 Size: 2295 6610 SfoI GGC'GCC Cuts at: 2779 6057 2779 Size: 3278 5627 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8905 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8905 SmaI CCC'GGG Cuts at: 1872 5124 5325 6232 1872 Size: 3252 201 907 4545 Fragments arranged by size: 4545 3252 907 201 SmlI C'TyrA_G Cuts at: 820 1017 1943 2399 2987 4337 6092 7009 7910 Size: 197 926 456 588 1350 1755 917 901 Cuts at: 7910 8172 820 Size: 262 1553 Fragments arranged by size: 1755 1553 1350 926 917 901 588 456 262 197 SnaBI TAC'GTA Cuts at: 493 6202 6696 493 Size: 5709 494 2702 Fragments arranged by size: 5709 2702 494 SpeI A'CTAG_T Cuts at: 152 5032 152 Size: 4880 4025 SphI G_CATG'C Cuts at: 1132 6263 1132 Size: 5131 3774 SspI AAT'ATT Cuts at: 5 52 6829 5 Size: 47 6777 2081 Fragments arranged by size: 6777 2081 47 StuI AGG'CCT Cuts at: 1517 2366 5480 1517 Size: 849 3114 4942 Fragments arranged by size: 4942 3114 849 StyI C'CwwG_G Cuts at: 513 1065 2022 2277 4589 4743 4800 4956 5049 Size: 552 957 255 2312 154 57 156 93 Cuts at: 5049 5382 6344 513 Size: 333 962 3074 Fragments arranged by size: 3074 2312 962 957 552 333 255 156 154 93 57 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2961 3671 4201 5645 6092 6125 6932 Size: 1255 1381 710 530 1444 447 33 807 Cuts at: 6932 6949 7102 7287 7732 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1444 1381 1255 807 710 530 447 445 185 153 33 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3561 4341 4515 Size: 274 80 33 51 1443 1584 780 174 Cuts at: 4515 4827 5700 7151 8863 96 Size: 312 873 1451 1712 138 Fragments arranged by size: 1712 1584 1451 1443 873 780 312 274 174 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8865 8897 8865 Size: 32 8873 Tth111I GACn'n_nGTC Cuts at: 1316 3620 1316 Size: 2304 6601 XbaI T'CTAG_A Cuts at: 6241 6241 Size: 8905 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2902 3353 3548 4015 5056 5212 2902 Size: 451 195 467 1041 156 6595 Fragments arranged by size: 6595 1041 467 451 195 156 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8905 XmaI C'CCGG_G Cuts at: 1870 5122 5323 6230 1870 Size: 3252 201 907 4545 Fragments arranged by size: 4545 3252 907 201 XmnI GAAnn'nnTTC Cuts at: 7034 7034 Size: 8905 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4431 6713 276 Size: 53 83 186 936 2897 2282 2468 Fragments arranged by size: 2897 2468 2282 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BstZ17I EcoRV FseI FspAI NruI PacI PspXI PsrI RsrII SrfI SwaI