Restriction map for
pFN21ASDA1337
(Linear) (Six-base) MAPSORT of: KIAA1337.seq Check: 8310 from: 1 to: 4176
KIAA1337.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3063 4176
Size: 3063 1113
AatII G_ACGT'C
Cuts at: 0 2410 4176
Size: 2410 1766
AccI GT'mk_AC
Cuts at: 0 1309 1430 2531 2665 3113 3373 4176
Size: 1309 121 1101 134 448 260 803
Fragments arranged by size:
1309 1101 803 448 260 134 121
Acc65I G'GTAC_C
Cuts at: 0 692 4176
Size: 692 3484
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 1189 2742 3209 4176
Size: 427 762 1553 467 967
Fragments arranged by size:
1553 967 762 467 427
AflII C'TTAA_G
Cuts at: 0 376 4176
Size: 376 3800
AflIII A'CryG_T
Cuts at: 0 1937 2057 4176
Size: 1937 120 2119
Fragments arranged by size:
2119 1937 120
AleI CACnn'nnGTG
Cuts at: 0 1285 3979 4176
Size: 1285 2694 197
Fragments arranged by size:
2694 1285 197
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2725 3295 4033 4176
Size: 2725 570 738 143
Fragments arranged by size:
2725 738 570 143
AlwNI CAG_nnn'CTG
Cuts at: 0 474 675 1080 1412 2145 2304 3219 3450
Size: 474 201 405 332 733 159 915 231
Cuts at: 3450 4176
Size: 726
Fragments arranged by size:
915 733 726 474 405 332 231 201
159
ApaI G_GGCC'C
Cuts at: 0 93 107 1984 2268 3213 4104 4176
Size: 93 14 1877 284 945 891 72
Fragments arranged by size:
1877 945 891 284 93 72 14
AvaI C'yCGr_G
Cuts at: 0 506 1951 2080 3099 3283 3300 3463 4176
Size: 506 1445 129 1019 184 17 163 713
Fragments arranged by size:
1445 1019 713 506 184 163 129 17
BamHI G'GATC_C
Cuts at: 0 391 4176
Size: 391 3785
BanI G'GyrC_C
Cuts at: 0 692 754 1860 2485 2598 2853 3149 4032
Size: 692 62 1106 625 113 255 296 883
Cuts at: 4032 4081 4114 4176
Size: 49 33 62
Fragments arranged by size:
1106 883 692 625 296 255 113 62
62 49 33
BanII G_rGCy'C
Cuts at: 0 93 107 513 830 985 1099 1177 1876
Size: 93 14 406 317 155 114 78 699
Cuts at: 1876 1984 2268 2695 3213 3290 3472 4104 4176
Size: 108 284 427 518 77 182 632 72
Fragments arranged by size:
699 632 518 427 406 317 284 182
155 114 108 93 78 77 72 14
BbeI G_GCGC'C
Cuts at: 0 758 4036 4176
Size: 758 3278 140
Fragments arranged by size:
3278 758 140
BbsI GAAGACnn'nnnn_
Cuts at: 0 662 1649 1731 1918 3033 4176
Size: 662 987 82 187 1115 1143
Fragments arranged by size:
1143 1115 987 662 187 82
BbvCI CC'TCA_GC
Cuts at: 0 3637 4176
Size: 3637 539
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3156 3190 4176
Size: 3156 34 986
Fragments arranged by size:
3156 986 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1543 3009 4176
Size: 1543 1466 1167
Fragments arranged by size:
1543 1466 1167
BfrBI ATG'CAT
Cuts at: 0 2849 4176
Size: 2849 1327
BglI GCCn_nnn'nGGC
Cuts at: 0 1297 2263 3592 4176
Size: 1297 966 1329 584
Fragments arranged by size:
1329 1297 966 584
BglII A'GATC_T
Cuts at: 0 1052 4176
Size: 1052 3124
BlpI GC'TnA_GC
Cuts at: 0 676 2872 4176
Size: 676 2196 1304
Fragments arranged by size:
2196 1304 676
Bme1580I G_kGCm'C
Cuts at: 0 93 107 747 1984 2268 2603 2933 3152
Size: 93 14 640 1237 284 335 330 219
Cuts at: 3152 3213 3891 3917 4104 4176
Size: 61 678 26 187 72
Fragments arranged by size:
1237 678 640 335 330 284 219 187
93 72 61 26 14
BmgBI CAC'GTC
Cuts at: 0 1938 2058 3087 4176
Size: 1938 120 1029 1089
Fragments arranged by size:
1938 1089 1029 120
BmrI ACTGGGnnnn_n'
Cuts at: 0 378 2166 2808 3207 4176
Size: 378 1788 642 399 969
Fragments arranged by size:
1788 969 642 399 378
BmtI G_CTAG'C
Cuts at: 0 2453 4176
Size: 2453 1723
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1170 2358 2619 4176
Size: 1170 1188 261 1557
Fragments arranged by size:
1557 1188 1170 261
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 201 649 1123 1866 1888 2038 2065 2434
Size: 201 448 474 743 22 150 27 369
Cuts at: 2434 2665 2862 3424 3702 3806 3867 3868 4176
Size: 231 197 562 278 104 61 1 308
Fragments arranged by size:
743 562 474 448 369 308 278 231
201 197 150 104 61 27 22 1
Bpu10I CC'TnA_GC
Cuts at: 0 2326 2433 3637 4176
Size: 2326 107 1204 539
Fragments arranged by size:
2326 1204 539 107
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 949 2299 4054 4176
Size: 949 1350 1755 122
Fragments arranged by size:
1755 1350 949 122
BsaI GGTCTCn'nnnn_
Cuts at: 0 610 1492 3089 4176
Size: 610 882 1597 1087
Fragments arranged by size:
1597 1087 882 610
BsaAI yAC'GTr
Cuts at: 0 1287 1521 2769 3380 3864 4176
Size: 1287 234 1248 611 484 312
Fragments arranged by size:
1287 1248 611 484 312 234
BsaHI Gr'CG_yC
Cuts at: 0 755 2407 3056 3329 3818 4033 4176
Size: 755 1652 649 273 489 215 143
Fragments arranged by size:
1652 755 649 489 273 215 143
BsaWI w'CCGG_w
Cuts at: 0 2402 4176
Size: 2402 1774
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 415 445 1726 1756 2524 2554 3223 3253
Size: 415 30 1281 30 768 30 669 30
Cuts at: 3253 4176
Size: 923
Fragments arranged by size:
1281 923 768 669 415 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 58 61 70 3231 3625 3729 4176
Size: 58 3 9 3161 394 104 447
Fragments arranged by size:
3161 447 394 104 58 9 3
BseYI C'CCAG_C
Cuts at: 0 165 194 220 700 764 978 1006 1241
Size: 165 29 26 480 64 214 28 235
Cuts at: 1241 1752 2049 2186 3425 3689 4020 4036 4110
Size: 511 297 137 1239 264 331 16 74
Cuts at: 4110 4176
Size: 66
Fragments arranged by size:
1239 511 480 331 297 264 235 214
165 137 74 66 64 29 28 26
16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1906 2134 2152 3367 3559 4176
Size: 1906 228 18 1215 192 617
Fragments arranged by size:
1906 1215 617 228 192 18
BsiHKAI G_wGCw'C
Cuts at: 0 233 830 965 1177 1209 1876 3506 3635
Size: 233 597 135 212 32 667 1630 129
Cuts at: 3635 4130 4176
Size: 495 46
Fragments arranged by size:
1630 667 597 495 233 212 135 129
46 32
BsiWI C'GTAC_G
Cuts at: 0 3841 4176
Size: 3841 335
BsmBI CGTCTCn'nnnn_
Cuts at: 0 430 1928 2048 2214 2947 3885 4176
Size: 430 1498 120 166 733 938 291
Fragments arranged by size:
1498 938 733 430 291 166 120
Bsp1286I G_dGCh'C
Cuts at: 0 93 107 233 513 747 830 965 985
Size: 93 14 126 280 234 83 135 20
Cuts at: 985 1099 1177 1209 1876 1984 2268 2603 2695
Size: 114 78 32 667 108 284 335 92
Cuts at: 2695 2933 3152 3213 3290 3472 3506 3635 3891
Size: 238 219 61 77 182 34 129 256
Cuts at: 3891 3917 4104 4130 4176
Size: 26 187 26 46
Fragments arranged by size:
667 335 284 280 256 238 234 219
187 182 135 129 126 114 108 93
92 83 78 77 61 46 34 32
26 26 20 14
BspEI T'CCGG_A
Cuts at: 0 2402 4176
Size: 2402 1774
BspHI T'CATG_A
Cuts at: 0 1016 4176
Size: 1016 3160
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1947 3063 3302 3428 3505 3785 3803 4176
Size: 1947 1116 239 126 77 280 18 373
Fragments arranged by size:
1947 1116 373 280 239 126 77 18
BsrBI CCG'CTC
Cuts at: 0 491 599 3686 4139 4176
Size: 491 108 3087 453 37
Fragments arranged by size:
3087 491 453 108 37
BsrDI GCAATG_nn'
Cuts at: 0 1415 4176
Size: 1415 2761
BsrFI r'CCGG_y
Cuts at: 0 407 2243 2453 2994 4176
Size: 407 1836 210 541 1182
Fragments arranged by size:
1836 1182 541 407 210
BsrGI T'GTAC_A
Cuts at: 0 2492 4176
Size: 2492 1684
BssHII G'CGCG_C
Cuts at: 0 777 841 4176
Size: 777 64 3335
Fragments arranged by size:
3335 777 64
BssSI C'ACGA_G
Cuts at: 0 337 955 4123 4176
Size: 337 618 3168 53
Fragments arranged by size:
3168 618 337 53
BstAPI GCAn_nnn'nTGC
Cuts at: 0 474 1412 2145 2345 3258 3450 3459 3656
Size: 474 938 733 200 913 192 9 197
Cuts at: 3656 4176
Size: 520
Fragments arranged by size:
938 913 733 520 474 200 197 192
9
BstEII G'GTnAC_C
Cuts at: 0 1278 3666 4176
Size: 1278 2388 510
Fragments arranged by size:
2388 1278 510
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1638 1768 3091 4176
Size: 1638 130 1323 1085
Fragments arranged by size:
1638 1323 1085 130
BstYI r'GATC_y
Cuts at: 0 326 391 633 969 1052 1193 1748 3353
Size: 326 65 242 336 83 141 555 1605
Cuts at: 3353 4176
Size: 823
Fragments arranged by size:
1605 823 555 336 326 242 141 83
65
Bsu36I CC'TnA_GG
Cuts at: 0 2459 4176
Size: 2459 1717
BtgI C'CryG_G
Cuts at: 0 254 527 569 2115 2835 3026 3315 3359
Size: 254 273 42 1546 720 191 289 44
Cuts at: 3359 3587 3743 3896 4176
Size: 228 156 153 280
Fragments arranged by size:
1546 720 289 280 273 254 228 191
156 153 44 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3159 3888 3918 4176
Size: 3159 729 30 258
Fragments arranged by size:
3159 729 258 30
BtsI GCAGTG_nn'
Cuts at: 0 1194 3329 3355 4176
Size: 1194 2135 26 821
Fragments arranged by size:
2135 1194 821 26
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1882 1917 4176
Size: 1882 35 2259
Fragments arranged by size:
2259 1882 35
DraIII CAC_nnn'GTG
Cuts at: 0 960 4176
Size: 960 3216
DrdI GACnn_nn'nnGTC
Cuts at: 0 3085 4176
Size: 3085 1091
EaeI y'GGCC_r
Cuts at: 0 419 728 1109 1289 1298 1562 1808 2118
Size: 419 309 381 180 9 264 246 310
Cuts at: 2118 2771 3004 3584 3593 3872 4176
Size: 653 233 580 9 279 304
Fragments arranged by size:
653 580 419 381 310 309 304 279
264 246 233 180 9 9
EarI CTCTTCn'nnn_
Cuts at: 0 238 1165 1513 2024 2281 2416 2503 3139
Size: 238 927 348 511 257 135 87 636
Cuts at: 3139 3925 4176
Size: 786 251
Fragments arranged by size:
927 786 636 511 348 257 251 238
135 87
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 417 1099 2239 4176
Size: 417 682 1140 1937
Fragments arranged by size:
1937 1140 682 417
EcoICRI GAG'CTC
Cuts at: 0 828 1175 1874 4176
Size: 828 347 699 2302
Fragments arranged by size:
2302 828 699 347
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 201 427 649 1123 1189 1866 1888 2038
Size: 201 226 222 474 66 677 22 150
Cuts at: 2038 2065 2434 2665 2742 2862 3209 3424 3702
Size: 27 369 231 77 120 347 215 278
Cuts at: 3702 3806 3867 3868 4176
Size: 104 61 1 308
Fragments arranged by size:
677 474 369 347 308 278 231 226
222 215 201 150 120 104 77 66
61 27 22 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 116 2897 3136 3166 3766 4176
Size: 116 2781 239 30 600 410
Fragments arranged by size:
2781 600 410 239 116 30
EcoO109I rG'GnC_Cy
Cuts at: 0 89 103 104 550 1077 1149 1980 1981
Size: 89 14 1 446 527 72 831 1
Cuts at: 1981 2160 2265 2439 3209 4074 4100 4101 4166
Size: 179 105 174 770 865 26 1 65
Cuts at: 4166 4176
Size: 10
Fragments arranged by size:
865 831 770 527 446 179 174 105
89 72 65 26 14 10 1 1
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1710 2772 2823 2847 4176
Size: 1710 1062 51 24 1329
Fragments arranged by size:
1710 1329 1062 51 24
FspI TGC'GCA
Cuts at: 0 1186 1366 1657 4176
Size: 1186 180 291 2519
Fragments arranged by size:
2519 1186 291 180
HaeII r_GCGC'y
Cuts at: 0 376 450 758 882 2333 2626 3548 3834
Size: 376 74 308 124 1451 293 922 286
Cuts at: 3834 4036 4176
Size: 202 140
Fragments arranged by size:
1451 922 376 308 293 286 202 140
124 74
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 415 447 2023 2055 4176
Size: 415 32 1576 32 2121
Fragments arranged by size:
2121 1576 415 32 32
HincII GTy'rAC
Cuts at: 0 3060 3374 4176
Size: 3060 314 802
Fragments arranged by size:
3060 802 314
KasI G'GCGC_C
Cuts at: 0 754 4032 4176
Size: 754 3278 144
Fragments arranged by size:
3278 754 144
KpnI G_GTAC'C
Cuts at: 0 696 4176
Size: 696 3480
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1941 2126 4176
Size: 1941 185 2050
Fragments arranged by size:
2050 1941 185
MscI TGG'CCA
Cuts at: 0 1111 1291 1300 2773 3874 4176
Size: 1111 180 9 1473 1101 302
Fragments arranged by size:
1473 1111 1101 302 180 9
MslI CAynn'nnrTG
Cuts at: 0 253 1285 1588 2299 3580 3979 4176
Size: 253 1032 303 711 1281 399 197
Fragments arranged by size:
1281 1032 711 399 303 253 197
MspA1I CmG'CkG
Cuts at: 0 294 465 474 529 571 672 978 1241
Size: 294 171 9 55 42 101 306 263
Cuts at: 1241 1412 1493 1653 1889 2117 2136 2186 2527
Size: 171 81 160 236 228 19 50 341
Cuts at: 2527 3309 3450 3589 4176
Size: 782 141 139 587
Fragments arranged by size:
782 587 341 306 294 263 236 228
171 171 160 141 139 101 81 55
50 42 19 9
NaeI GCC'GGC
Cuts at: 0 2245 2455 2996 4176
Size: 2245 210 541 1180
Fragments arranged by size:
2245 1180 541 210
NarI GG'CG_CC
Cuts at: 0 755 4033 4176
Size: 755 3278 143
Fragments arranged by size:
3278 755 143
NcoI C'CATG_G
Cuts at: 0 254 3026 3359 4176
Size: 254 2772 333 817
Fragments arranged by size:
2772 817 333 254
NgoMIV G'CCGG_C
Cuts at: 0 2243 2453 2994 4176
Size: 2243 210 541 1182
Fragments arranged by size:
2243 1182 541 210
NheI G'CTAG_C
Cuts at: 0 2449 4176
Size: 2449 1727
NsiI A_TGCA'T
Cuts at: 0 2851 4176
Size: 2851 1325
NspI r_CATG'y
Cuts at: 0 1389 4176
Size: 1389 2787
PasI CC'CwG_GG
Cuts at: 0 189 1977 2444 4077 4104 4176
Size: 189 1788 467 1633 27 72
Fragments arranged by size:
1788 1633 467 189 72 27
PflMI CCAn_nnn'nTGG
Cuts at: 0 1080 1542 4176
Size: 1080 462 2634
Fragments arranged by size:
2634 1080 462
PfoI T'CCnGG_A
Cuts at: 0 932 2369 4176
Size: 932 1437 1807
Fragments arranged by size:
1807 1437 932
PmlI CAC'GTG
Cuts at: 0 1521 3864 4176
Size: 1521 2343 312
Fragments arranged by size:
2343 1521 312
PpuMI rG'GwC_Cy
Cuts at: 0 1077 2160 2439 4176
Size: 1077 1083 279 1737
Fragments arranged by size:
1737 1083 1077 279
PspOMI G'GGCC_C
Cuts at: 0 89 103 1980 2264 3209 4100 4176
Size: 89 14 1877 284 945 891 76
Fragments arranged by size:
1877 945 891 284 89 76 14
PstI C_TGCA'G
Cuts at: 0 29 247 470 1161 1683 1833 2093 2141
Size: 29 218 223 691 522 150 260 48
Cuts at: 2141 2393 2474 3254 3455 4136 4176
Size: 252 81 780 201 681 40
Fragments arranged by size:
780 691 681 522 260 252 223 218
201 150 81 48 40 29
PvuII CAG'CTG
Cuts at: 0 465 474 1412 1493 1653 1889 2136 3309
Size: 465 9 938 81 160 236 247 1173
Cuts at: 3309 3450 4176
Size: 141 726
Fragments arranged by size:
1173 938 726 465 247 236 160 141
81 9
SacI G_AGCT'C
Cuts at: 0 830 1177 1876 4176
Size: 830 347 699 2300
Fragments arranged by size:
2300 830 699 347
SacII CC_GC'GG
Cuts at: 0 530 572 2118 3590 4176
Size: 530 42 1546 1472 586
Fragments arranged by size:
1546 1472 586 530 42
SalI G'TCGA_C
Cuts at: 0 3372 4176
Size: 3372 804
SanDI GG'GwC_CC
Cuts at: 0 2439 4176
Size: 2439 1737
SapI GCTCTTCn'nnn_
Cuts at: 0 238 1165 2024 4176
Size: 238 927 859 2152
Fragments arranged by size:
2152 927 859 238
SbfI CC_TGCA'GG
Cuts at: 0 2474 4176
Size: 2474 1702
ScaI AGT'ACT
Cuts at: 0 1540 4176
Size: 1540 2636
SexAI A'CCwGG_T
Cuts at: 0 3761 4176
Size: 3761 415
SfcI C'TryA_G
Cuts at: 0 25 243 466 1157 1679 1829 2089 2137
Size: 25 218 223 691 522 150 260 48
Cuts at: 2137 2389 2470 2532 2666 3231 3250 3451 4132
Size: 252 81 62 134 565 19 201 681
Cuts at: 4132 4176
Size: 44
Fragments arranged by size:
691 681 565 522 260 252 223 218
201 150 134 81 62 48 44 25
19
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1297 3592 4176
Size: 1297 2295 584
Fragments arranged by size:
2295 1297 584
SfoI GGC'GCC
Cuts at: 0 756 4034 4176
Size: 756 3278 142
Fragments arranged by size:
3278 756 142
SmaI CCC'GGG
Cuts at: 0 3101 3302 4176
Size: 3101 201 874
Fragments arranged by size:
3101 874 201
SmlI C'TyrA_G
Cuts at: 0 376 964 2314 4069 4176
Size: 376 588 1350 1755 107
Fragments arranged by size:
1755 1350 588 376 107
SpeI A'CTAG_T
Cuts at: 0 3009 4176
Size: 3009 1167
StuI AGG'CCT
Cuts at: 0 343 3457 4176
Size: 343 3114 719
Fragments arranged by size:
3114 719 343
StyI C'CwwG_G
Cuts at: 0 254 2566 2720 2777 2933 3026 3359 4176
Size: 254 2312 154 57 156 93 333 817
Fragments arranged by size:
2312 817 333 254 156 154 93 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 938 1648 2178 3622 4069 4102 4176
Size: 938 710 530 1444 447 33 74
Fragments arranged by size:
1444 938 710 530 447 74 33
TatI w'GTAC_w
Cuts at: 0 1538 2318 2492 2804 3677 4176
Size: 1538 780 174 312 873 499
Fragments arranged by size:
1538 873 780 499 312 174
Tth111I GACn'n_nGTC
Cuts at: 0 1597 4176
Size: 1597 2579
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 879 1330 1525 1992 3033 3189 4176
Size: 879 451 195 467 1041 156 987
Fragments arranged by size:
1041 987 879 467 451 195 156
XmaI C'CCGG_G
Cuts at: 0 3099 3300 4176
Size: 3099 201 876
Fragments arranged by size:
3099 876 201
ZraI GAC'GTC
Cuts at: 0 2408 4176
Size: 2408 1768
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflII AflIII AleI
AlfI AlwNI ApaI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
FspI HaeII Hin4I HincII KasI KpnI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NsiI
NspI PasI PflMI PfoI PmlI PpuMI PspOMI PstI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SmaI SmlI SpeI StuI
StyI TaqII TatI Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
AclI AfeI AgeI AhdI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BclI BsaBI BsiEI BsmI
BstBI BstZ17I ClaI DraI EagI EcoRI EcoRV FseI
FspAI HindIII HpaI MfeI MluI NdeI NotI NruI
PacI PciI PmeI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SgfI SgrAI SnaBI SphI SrfI SspI
SwaI TstI XbaI XhoI XmnI