(Circular) (Six-base) MAPSORT of: pFN21ASDA1334.seq Check: 5568 from: 1 to: 7669 pFN21ASDA1334 7669 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5479 278 Size: 53 83 186 936 3943 2468 Fragments arranged by size: 3943 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5012 1937 Size: 3075 4594 Acc65I G'GTAC_C Cuts at: 4372 4990 4372 Size: 618 7051 AclI AA'CG_TT Cuts at: 1563 5796 6169 1563 Size: 4233 373 3063 Fragments arranged by size: 4233 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2324 3066 3387 4940 5732 7116 7384 1632 Size: 692 742 321 1553 792 1384 268 1917 Fragments arranged by size: 1917 1553 1384 792 742 692 321 268 AflII C'TTAA_G Cuts at: 820 1017 4283 820 Size: 197 3266 4206 Fragments arranged by size: 4206 3266 197 AflIII A'CryG_T Cuts at: 1203 2215 4089 6568 7545 1203 Size: 1012 1874 2479 977 1327 Fragments arranged by size: 2479 1874 1327 1012 977 AgeI A'CCGG_T Cuts at: 6500 6500 Size: 7669 AhdI GACnn_n'nnGTC Cuts at: 6398 6398 Size: 7669 AleI CACnn'nnGTG Cuts at: 1166 2172 1166 Size: 1006 6663 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7669 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4709 4741 4709 Size: 32 7637 AlwNI CAG_nnn'CTG Cuts at: 3020 3322 3637 4774 5412 6984 3020 Size: 302 315 1137 638 1572 3705 Fragments arranged by size: 3705 1572 1137 638 315 302 ApaI G_GGCC'C Cuts at: 3209 3209 Size: 7669 ApaLI G'TGCA_C Cuts at: 5726 6882 5726 Size: 1156 6513 ApoI r'AATT_y Cuts at: 1493 2718 3312 4586 4978 5204 5356 7465 1493 Size: 1225 594 1274 392 226 152 2109 1697 Fragments arranged by size: 2109 1697 1274 1225 594 392 226 152 AseI AT'TA_AT Cuts at: 160 1032 6223 160 Size: 872 5191 1606 Fragments arranged by size: 5191 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7669 AvaI C'yCGr_G Cuts at: 1870 1943 4994 1870 Size: 73 3051 4545 Fragments arranged by size: 4545 3051 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4364 4397 4364 Size: 33 7636 BamHI G'GATC_C Cuts at: 4999 5366 4999 Size: 367 7302 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4372 4990 6445 618 Size: 325 217 627 2585 618 1455 1842 Fragments arranged by size: 2585 1842 1455 627 618 325 217 BanII G_rGCy'C Cuts at: 1391 2453 3209 3365 4640 4988 1391 Size: 1062 756 156 1275 348 4072 Fragments arranged by size: 4072 1275 1062 756 348 156 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4374 928 Size: 971 2475 4223 Fragments arranged by size: 4223 2475 971 BbvCI CC'TCA_GC Cuts at: 3464 3596 3903 3464 Size: 132 307 7230 Fragments arranged by size: 7230 307 132 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5860 5894 1903 Size: 34 3923 34 3678 Fragments arranged by size: 3923 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2669 5561 6771 2669 Size: 2892 1210 3567 Fragments arranged by size: 3567 2892 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7669 BfrBI ATG'CAT Cuts at: 2325 3647 4867 2325 Size: 1322 1220 5127 Fragments arranged by size: 5127 1322 1220 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6280 136 Size: 107 122 71 631 605 4608 1525 Fragments arranged by size: 4608 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2520 3061 3340 7664 2520 Size: 541 279 4324 2525 Fragments arranged by size: 4324 2525 541 279 BlpI GC'TnA_GC Cuts at: 3993 5086 3993 Size: 1093 6576 Bme1580I G_kGCm'C Cuts at: 1790 3209 5730 6886 1790 Size: 1419 2521 1156 2573 Fragments arranged by size: 2573 2521 1419 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7669 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2866 5400 6358 Size: 416 53 83 399 24 1449 2534 958 Cuts at: 6358 442 Size: 1753 Fragments arranged by size: 2534 1753 1449 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7669 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3472 4038 3472 Size: 566 7103 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4019 4346 4382 4670 4790 4796 6329 1239 Size: 2780 327 36 288 120 6 1533 2579 Fragments arranged by size: 2780 2579 1533 327 288 120 36 6 Bpu10I CC'TnA_GC Cuts at: 2406 3167 3464 3596 3903 4599 2406 Size: 761 297 132 307 696 5476 Fragments arranged by size: 5476 761 696 307 297 132 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5794 6659 6957 5794 Size: 865 298 6506 Fragments arranged by size: 6506 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4510 6332 882 Size: 617 3011 1822 2219 Fragments arranged by size: 3011 2219 1822 617 BsaAI yAC'GTr Cuts at: 493 1204 2218 4966 5460 493 Size: 711 1014 2748 494 2702 Fragments arranged by size: 2748 2702 1014 711 494 BsaBI GATnn'nnATC Cuts at: 7663 7663 Size: 7669 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5476 5858 6493 275 Size: 53 83 186 936 3943 382 635 1451 Fragments arranged by size: 3943 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2276 6102 6500 6774 6921 7332 2276 Size: 3826 398 274 147 411 2613 Fragments arranged by size: 3826 2613 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2538 2568 2896 2926 4709 Size: 30 165 30 1135 30 328 30 1783 Cuts at: 4709 4739 1178 Size: 30 4108 Fragments arranged by size: 4108 1783 1135 328 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3515 1186 Size: 2329 5340 BseYI C'CCAG_C Cuts at: 2353 4164 4728 6872 7368 2353 Size: 1811 564 2144 496 2654 Fragments arranged by size: 2654 2144 1811 564 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2447 2667 3548 4904 1163 Size: 573 711 220 881 1356 3928 Fragments arranged by size: 3928 1356 881 711 573 220 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5119 5880 6908 1540 Size: 275 186 3118 761 1028 2301 Fragments arranged by size: 3118 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3365 4640 5730 5815 6886 3365 Size: 1275 1090 85 1071 4148 Fragments arranged by size: 4148 1275 1090 1071 85 BsmI GAATG_Cn' Cuts at: 2228 2327 3649 4829 4869 5180 5273 2228 Size: 99 1322 1180 40 311 93 4624 Fragments arranged by size: 4624 1322 1180 311 99 93 40 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2453 3209 3365 4640 4988 5730 5815 Size: 399 663 756 156 1275 348 742 85 Cuts at: 5815 6886 1391 Size: 1071 2174 Fragments arranged by size: 2174 1275 1071 756 742 663 399 348 156 85 BspEI T'CCGG_A Cuts at: 7332 7332 Size: 7669 BspHI T'CATG_A Cuts at: 2235 3846 5558 7288 2235 Size: 1611 1712 1730 2616 Fragments arranged by size: 2616 1730 1712 1611 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5024 844 Size: 4180 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5556 1394 Size: 429 3733 3507 Fragments arranged by size: 3733 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4374 6164 6338 65 Size: 1183 3126 1790 174 1396 Fragments arranged by size: 3126 1790 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2085 6313 6500 1700 Size: 110 275 4228 187 2869 Fragments arranged by size: 4228 2869 275 187 110 BsrGI T'GTAC_A Cuts at: 96 2500 3975 96 Size: 2404 1475 3790 Fragments arranged by size: 3790 2404 1475 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7669 BssSI C'ACGA_G Cuts at: 3590 5729 6741 3590 Size: 2139 1012 4518 Fragments arranged by size: 4518 2139 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3089 3637 4112 794 Size: 2295 548 475 4351 Fragments arranged by size: 4351 2295 548 475 BstBI TT'CG_AA Cuts at: 2007 6473 2007 Size: 4466 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7669 BstYI r'GATC_y Cuts at: 1972 2520 3061 3340 3634 4999 5366 5751 5768 Size: 548 541 279 294 1365 367 385 17 Cuts at: 5768 7220 7306 7318 7664 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1365 548 541 385 367 346 294 279 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2228 2228 Size: 7669 BtgI C'CryG_G Cuts at: 513 1065 2100 513 Size: 552 1035 6082 Fragments arranged by size: 6082 1035 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6930 Fragments arranged by size: 6930 683 56 BtsI GCAGTG_nn' Cuts at: 2132 5185 5979 5999 2132 Size: 3053 794 20 3802 Fragments arranged by size: 3802 3053 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7669 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7634 DraI TTT'AAA Cuts at: 2763 4973 2763 Size: 2210 5459 DraIII CAC_nnn'GTG Cuts at: 2249 2249 Size: 7669 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3305 4090 6676 809 Size: 507 1989 785 2586 1802 Fragments arranged by size: 2586 1989 1802 785 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5116 6005 7634 Size: 54 1425 50 275 18 3286 889 1629 Cuts at: 7634 8 Size: 43 Fragments arranged by size: 3286 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5116 1537 Size: 275 3304 4090 Fragments arranged by size: 4090 3304 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2021 2045 2893 3344 3603 3738 4007 4338 Size: 668 24 848 451 259 135 269 331 Cuts at: 4338 4645 5599 1353 Size: 307 954 3423 Fragments arranged by size: 3423 954 848 668 451 331 307 269 259 135 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3170 4835 6256 6630 6776 387 Size: 1064 138 1581 1665 1421 374 146 1280 Fragments arranged by size: 1665 1581 1421 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3363 4638 3363 Size: 1275 6394 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2324 3066 3387 4019 4346 4382 4670 Size: 393 692 742 321 632 327 36 288 Cuts at: 4670 4790 4796 4940 5732 6329 7116 7384 1239 Size: 120 6 144 792 597 787 268 1524 Fragments arranged by size: 1524 792 787 742 692 632 597 393 327 321 288 268 144 120 36 6 EcoRI G'AATT_C Cuts at: 2718 3312 4978 5356 2718 Size: 594 1666 378 5031 Fragments arranged by size: 5031 1666 594 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3536 4729 3536 Size: 1193 6476 FspI TGC'GCA Cuts at: 6175 6175 Size: 7669 HaeII r_GCGC'y Cuts at: 1678 2289 4784 6816 1678 Size: 611 2495 2032 2531 Fragments arranged by size: 2531 2495 2032 611 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2538 2570 2783 2815 3053 Size: 32 135 32 966 32 213 32 238 Cuts at: 3053 3085 3236 3268 4103 4135 4835 4867 6310 Size: 32 151 32 835 32 700 32 1443 Cuts at: 6310 6342 6384 6416 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1443 966 835 700 238 213 151 135 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2368 4161 5013 5259 669 Size: 988 281 430 1793 852 246 3079 Fragments arranged by size: 3079 1793 988 852 430 281 246 HindIII A'AGCT_T Cuts at: 748 3169 748 Size: 2421 5248 HpaI GTT'AAC Cuts at: 5259 5259 Size: 7669 KpnI G_GTAC'C Cuts at: 4376 4994 4376 Size: 618 7051 MfeI C'AATT_G Cuts at: 4079 5268 4079 Size: 1189 6480 MluI A'CGCG_T Cuts at: 7545 7545 Size: 7669 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6783 6967 1305 Size: 5478 184 2007 Fragments arranged by size: 5478 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5847 Fragments arranged by size: 5847 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2172 5627 5986 6145 518 Size: 648 1006 3455 359 159 2042 Fragments arranged by size: 3455 2042 1006 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2402 2488 3973 4040 4774 4816 5085 5762 Size: 796 86 1485 67 734 42 269 677 Cuts at: 5762 6910 7155 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1485 1148 796 734 677 269 245 86 67 42 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7117 NdeI CA'TA_TG Cuts at: 387 387 Size: 7669 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7669 NotI GC'GGCC_GC Cuts at: 5116 5116 Size: 7669 NsiI A_TGCA'T Cuts at: 2327 3649 4869 2327 Size: 1322 1220 5127 Fragments arranged by size: 5127 1322 1220 NspI r_CATG'y Cuts at: 1132 1229 5027 6572 1132 Size: 97 3798 1545 2229 Fragments arranged by size: 3798 2229 1545 97 PacI TTA_AT'TAA Cuts at: 2637 2637 Size: 7669 PasI CC'CwG_GG Cuts at: 1348 3214 1348 Size: 1866 5803 PciI A'CATG_T Cuts at: 6568 6568 Size: 7669 PflMI CCAn_nnn'nTGG Cuts at: 1108 2067 1108 Size: 959 6710 PfoI T'CCnGG_A Cuts at: 2535 2561 2535 Size: 26 7643 PmeI GTTT'AAAC Cuts at: 4973 4973 Size: 7669 PmlI CAC'GTG Cuts at: 2218 2218 Size: 7669 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4190 4222 4709 4741 5738 5770 7251 7283 4190 Size: 32 487 32 997 32 1481 32 4576 Fragments arranged by size: 4576 1481 997 487 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7669 PsiI TTA'TAA Cuts at: 5239 5239 Size: 7669 PspOMI G'GGCC_C Cuts at: 3205 3205 Size: 7669 PstI C_TGCA'G Cuts at: 830 2097 3642 4252 4788 5021 830 Size: 1267 1545 610 536 233 3478 Fragments arranged by size: 3478 1545 1267 610 536 233 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7669 PvuII CAG'CTG Cuts at: 3973 4040 4774 4816 3973 Size: 67 734 42 6826 Fragments arranged by size: 6826 734 67 42 SacI G_AGCT'C Cuts at: 3365 4640 3365 Size: 1275 6394 SalI G'TCGA_C Cuts at: 1936 5011 1936 Size: 3075 4594 SapI GCTCTTCn'nnn_ Cuts at: 2021 4645 2021 Size: 2624 5045 SbfI CC_TGCA'GG Cuts at: 5021 5021 Size: 7669 ScaI AGT'ACT Cuts at: 3010 4917 5917 7629 3010 Size: 1907 1000 1712 3050 Fragments arranged by size: 3050 1907 1712 1000 SexAI A'CCwGG_T Cuts at: 3223 3223 Size: 7669 SfcI C'TryA_G Cuts at: 826 1045 2093 3012 3344 3386 3638 4248 4784 Size: 219 1048 919 332 42 252 610 536 Cuts at: 4784 5017 6152 6833 7024 826 Size: 233 1135 681 191 1471 Fragments arranged by size: 1471 1135 1048 919 681 610 536 332 252 233 219 191 42 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7669 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7669 SmaI CCC'GGG Cuts at: 1872 4996 1872 Size: 3124 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4283 5773 6674 6936 820 Size: 197 926 2340 1490 901 262 1553 Fragments arranged by size: 2340 1553 1490 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4966 5460 493 Size: 4473 494 2702 Fragments arranged by size: 4473 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7669 SphI G_CATG'C Cuts at: 1132 5027 1132 Size: 3895 3774 SspI AAT'ATT Cuts at: 5 52 4534 5593 5 Size: 47 4482 1059 2081 Fragments arranged by size: 4482 2081 1059 47 StuI AGG'CCT Cuts at: 1517 4712 1517 Size: 3195 4474 StyI C'CwwG_G Cuts at: 513 1065 3147 5108 513 Size: 552 2082 1961 3074 Fragments arranged by size: 3074 2082 1961 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3160 3206 3536 4368 5696 5713 5866 Size: 1255 1580 46 330 832 1328 17 153 Cuts at: 5866 6051 6496 325 Size: 185 445 1498 Fragments arranged by size: 1580 1498 1328 1255 832 445 330 185 153 46 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2500 3008 3975 Size: 274 80 33 51 1443 523 508 967 Cuts at: 3975 4915 5915 7627 96 Size: 940 1000 1712 138 Fragments arranged by size: 1712 1443 1000 967 940 523 508 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2557 2589 7629 7661 2557 Size: 32 5040 32 2565 Fragments arranged by size: 5040 2565 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2795 1316 Size: 1479 6190 XbaI T'CTAG_A Cuts at: 4024 5005 4024 Size: 981 6688 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7669 XmaI C'CCGG_G Cuts at: 1870 4994 1870 Size: 3124 4545 XmnI GAAnn'nnTTC Cuts at: 4533 5798 4533 Size: 1265 6404 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5477 276 Size: 53 83 186 936 3943 2468 Fragments arranged by size: 3943 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BbeI BsiWI BsmBI BstXI BstZ17I EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PpuMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI