Restriction map for
pFN21ASDA1325
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# NO CUT ENZYMES ( 165 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflIII AgeI AhdI AlwNI
AocI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BcgI BclI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsiYI BslI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I Cac8I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HaeIII HhaI Hin1I HinP1I HpaI
KasI KpnI KspI MamI MfeI
MflI MluI MluNI MroI MscI
MunI MwoI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tth111I TthHB8I Van91I
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccII 718 CGCG CG^CG
AflII 699 CTTAAG C^TTAAG
Alw44I 632 GTGCAC G^TGCAC
AlwI 803 GGATC GGATC(4/5)
ApaLI 632 GTGCAC G^TGCAC
AspHI 632 GTGCAC G[AT]GC[AT]^C
AvaI 535 CTCGGG C^[CT]CG[GA]G
AvaII 314 GGACC G^G[AT]CC
BbsI 917 GTCTTC GAAGAC(2/6)
BciVI 675 GGATAC GTATCC(6/5)
BcnI 864 CCCGG CC^[GC]GG
BfrI 699 CTTAAG C^TTAAG
BpmI 619 CTGGAG CTGGAG(16/14)
BpuAI 917 GTCTTC GAAGAC(2/6)
BsiHKAI 632 GTGCAC G[AT]GC[AT]^C
BsmI 591 GCATTC GAATGC(1/-1)
BsoBI 535 CTCGGG C^[CT]CG[GA]G
BssSI 745 CTCGTG CACGAG(-5/-1)
BstUI 718 CGCG CG^CG
Cfr13I 314 GGACC G^G[ACGT]CC
FauI 994 CCCGC CCCGC(4/6)
HgaI 325 GACGC GACGC(5/10)
HindIII 977 AAGCTT A^AGCTT
HphI 938 TCACC GGTGA(8/7)
MaeII 725 ACGT A^CGT
MaeIII 850 GTCAC ^GT[ACGT]AC
MspA1I 401 CAGCTG C[AC]G^C[GT]G
MvnI 718 CGCG CG^CG
NciI 864 CCCGG CC^[GC]GG
NspI 601 GCATGT [GA]CATG^[CT]
PleI 622 GAGTC GAGTC(4/5)
PstI 691 CTGCAG CTGCA^G
PvuII 401 CAGCTG CAG^CTG
SapI 419 GAAGAGC GCTCTTC(1/4)
Sau96I 314 GGACC G^G[ACGT]CC
SfcI 691 CTGCAG C^T[GA][CT]AG
Tsp45I 850 GTCAC ^GT[GC]AC
XbaI 321 TCTAGA T^CTAGA
XcmI 627 CCAGGGTGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AfaI 297 GTAC GT^AC
648 GTAC
BanII 559 GAGCCC G[GA]GC[CT]^C
640 GGGCTC
BsmAI 170 GAGAC GTCTC(1/5)
860 GTCTC
DdeI 53 CTCAG C^T[ACGT]AG
329 CTGAG
EarI 211 GAAGAG CTCTTC(1/4)
419 GAAGAG
EcoT14I 554 CCAAGG C^C[AT][AT]GG
563 CCAAGG
HapII 669 CCGG C^CGG
865 CCGG
HincII 6 GTTGAC GT[CT]^[GA]AC
197 GTCAAC
HindII 6 GTTGAC GT[CT]^[GA]AC
197 GTCAAC
HpaII 669 CCGG C^CGG
865 CCGG
Ksp632I 211 GAAGAG CTCTTC(1/4)
419 GAAGAG
MslI 831 CATCCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
885 CATGGCAATG
MspI 669 CCGG C^CGG
865 CCGG
NlaIV 313 GGGACC GG[ACGT]^[ACGT]CC
558 GGAGCC
RsaI 297 GTAC GT^AC
648 GTAC
SmlI 699 CTTAAG C^T[CT][GA]AG
734 CTCAAG
StyI 554 CCAAGG C^C[AT][AT]GG
563 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AcsI AluI ApoI BbvI
BfaI BmyI BsaJI BsmFI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
DpnII EcoRII Fnu4HI FokI HinfI
ItaI MaeI MboI MboII MnlI
MseI MvaI NdeII NlaIII Sau3AI
ScrFI SfaNI TfiI Tru9I TseI
Tsp509I TspRI