Restriction map for
pFN21ASDA1325
(Circular) (Six-base) MAPSORT of: pFN21ASDA1325.seq Check: 5464 from: 1 to: 5770
pFN21ASDA1325 5770 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3580 278
Size: 53 83 186 936 2044 2468
Fragments arranged by size:
2468 2044 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3113 1937
Size: 1176 4594
Acc65I G'GTAC_C
Cuts at: 3091 3091
Size: 5770
AclI AA'CG_TT
Cuts at: 1563 3897 4270 1563
Size: 2334 373 3063
Fragments arranged by size:
3063 2334 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2927 3833 5217 5485 1632
Size: 1295 906 1384 268 1917
Fragments arranged by size:
1917 1384 1295 906 268
AflII C'TTAA_G
Cuts at: 820 1017 2722 820
Size: 197 1705 3868
Fragments arranged by size:
3868 1705 197
AflIII A'CryG_T
Cuts at: 1203 4669 5646 1203
Size: 3466 977 1327
Fragments arranged by size:
3466 1327 977
AgeI A'CCGG_T
Cuts at: 4601 4601
Size: 5770
AhdI GACnn_n'nnGTC
Cuts at: 4499 4499
Size: 5770
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5770
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5770
AlwNI CAG_nnn'CTG
Cuts at: 3513 5085 3513
Size: 1572 4198
ApaLI G'TGCA_C
Cuts at: 2655 3827 4983 2655
Size: 1172 1156 3442
Fragments arranged by size:
3442 1172 1156
ApoI r'AATT_y
Cuts at: 1493 2099 2379 2417 2792 3060 3079 3305 3457
Size: 606 280 38 375 268 19 226 152
Cuts at: 3457 5566 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 606 375 280 268 226 152
38 19
AseI AT'TA_AT
Cuts at: 160 1032 4324 160
Size: 872 3292 1606
Fragments arranged by size:
3292 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5770
AvaI C'yCGr_G
Cuts at: 1870 1943 2558 3095 1870
Size: 73 615 537 4545
Fragments arranged by size:
4545 615 537 73
BamHI G'GATC_C
Cuts at: 3100 3467 3100
Size: 367 5403
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3091 4546 618
Size: 325 217 627 1304 1455 1842
Fragments arranged by size:
1842 1455 1304 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2586 2667 3089 1391
Size: 1195 81 422 4072
Fragments arranged by size:
4072 1195 422 81
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2933 928
Size: 971 1034 3765
Fragments arranged by size:
3765 1034 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3961 3995 1903
Size: 34 2024 34 3678
Fragments arranged by size:
3678 2024 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2692 3662 4872 2692
Size: 970 1210 3590
Fragments arranged by size:
3590 1210 970
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5770
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4381 136
Size: 107 122 71 631 605 2709 1525
Fragments arranged by size:
2709 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5765 5765
Size: 5770
BlpI GC'TnA_GC
Cuts at: 3187 3187
Size: 5770
Bme1580I G_kGCm'C
Cuts at: 1790 2659 3831 4987 1790
Size: 869 1172 1156 2573
Fragments arranged by size:
2573 1172 1156 869
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5770
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3501 4459 442
Size: 416 53 83 399 24 2084 958 1753
Fragments arranged by size:
2084 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5770
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2663 4430 1239
Size: 1424 1767 2579
Fragments arranged by size:
2579 1767 1424
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2742 3895 4760 5058 2742
Size: 1153 865 298 3454
Fragments arranged by size:
3454 1153 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4433 882
Size: 617 2934 2219
Fragments arranged by size:
2934 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3067 3561 493
Size: 711 1863 494 2702
Fragments arranged by size:
2702 1863 711 494
BsaBI GATnn'nnATC
Cuts at: 5764 5764
Size: 5770
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3577 3959 4594 275
Size: 53 83 186 936 2044 382 635 1451
Fragments arranged by size:
2044 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4203 4601 4875 5022 5433 4203
Size: 398 274 147 411 4540
Fragments arranged by size:
4540 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5545
Fragments arranged by size:
5545 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5770
BseYI C'CCAG_C
Cuts at: 2332 4973 5469 2332
Size: 2641 496 2633
Fragments arranged by size:
2641 2633 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5197
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3220 3981 5009 1540
Size: 275 186 1219 761 1028 2301
Fragments arranged by size:
2301 1219 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2659 3831 3916 4987 2659
Size: 1172 85 1071 3442
Fragments arranged by size:
3442 1172 1071 85
BsmI GAATG_Cn'
Cuts at: 2614 3281 3374 2614
Size: 667 93 5010
Fragments arranged by size:
5010 667 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2586 2659 2667 3089 3831 3916 4987
Size: 399 796 73 8 422 742 85 1071
Cuts at: 4987 1391
Size: 2174
Fragments arranged by size:
2174 1071 796 742 422 399 85 73
8
BspEI T'CCGG_A
Cuts at: 5433 5433
Size: 5770
BspHI T'CATG_A
Cuts at: 3659 5389 3659
Size: 1730 4040
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3125 844
Size: 2281 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3657 1394
Size: 429 1834 3507
Fragments arranged by size:
3507 1834 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2159 2638 2919 4265 4439 65
Size: 1183 911 479 281 1346 174 1396
Fragments arranged by size:
1396 1346 1183 911 479 281 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4414 4601 1700
Size: 110 2604 187 2869
Fragments arranged by size:
2869 2604 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5770
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5770
BssSI C'ACGA_G
Cuts at: 2768 3830 4842 2768
Size: 1062 1012 3696
Fragments arranged by size:
3696 1062 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5770
BstBI TT'CG_AA
Cuts at: 2007 4574 2007
Size: 2567 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5770
BstYI r'GATC_y
Cuts at: 1972 3100 3467 3852 3869 5321 5407 5419 5765
Size: 1128 367 385 17 1452 86 12 346
Cuts at: 5765 1972
Size: 1977
Fragments arranged by size:
1977 1452 1128 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5218
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5031
Fragments arranged by size:
5031 683 56
BtsI GCAGTG_nn'
Cuts at: 2723 3286 4080 4100 2723
Size: 563 794 20 4393
Fragments arranged by size:
4393 794 563 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5770
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5735
DraI TTT'AAA
Cuts at: 3074 3074
Size: 5770
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4777 809
Size: 507 3461 1802
Fragments arranged by size:
3461 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3217 4106 5735
Size: 54 1425 50 275 18 1387 889 1629
Cuts at: 5735 8
Size: 43
Fragments arranged by size:
1629 1425 1387 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3217 1537
Size: 275 1405 4090
Fragments arranged by size:
4090 1405 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2229 2437 3700 1353
Size: 876 208 1263 3423
Fragments arranged by size:
3423 1263 876 208
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4357 4731 4877 387
Size: 1064 138 2768 374 146 1280
Fragments arranged by size:
2768 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2663 2927 3833 4430 5217 5485 1239
Size: 393 1031 264 906 597 787 268 1524
Fragments arranged by size:
1524 1031 906 787 597 393 268 264
EcoRI G'AATT_C
Cuts at: 3079 3457 3079
Size: 378 5392
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2074 2074
Size: 5770
FspI TGC'GCA
Cuts at: 4276 4276
Size: 5770
HaeII r_GCGC'y
Cuts at: 1678 4917 1678
Size: 3239 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2673 2705 4411 4443 4485
Size: 32 135 32 1101 32 1706 32 42
Cuts at: 4485 4517 1373
Size: 32 2626
Fragments arranged by size:
2626 1706 1101 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2031 2222 3114 3360 669
Size: 988 281 93 191 892 246 3079
Fragments arranged by size:
3079 988 892 281 246 191 93
HindIII A'AGCT_T
Cuts at: 748 3000 748
Size: 2252 3518
HpaI GTT'AAC
Cuts at: 3360 3360
Size: 5770
KpnI G_GTAC'C
Cuts at: 3095 3095
Size: 5770
MfeI C'AATT_G
Cuts at: 3369 3369
Size: 5770
MluI A'CGCG_T
Cuts at: 5646 5646
Size: 5770
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4884 5068 1305
Size: 3579 184 2007
Fragments arranged by size:
3579 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3948
Fragments arranged by size:
3948 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2858 2912 3728 4087 4246 518
Size: 648 1692 54 816 359 159 2042
Fragments arranged by size:
2042 1692 816 648 359 159 54
MspA1I CmG'CkG
Cuts at: 1606 2426 3186 3863 5011 5256 1606
Size: 820 760 677 1148 245 2120
Fragments arranged by size:
2120 1148 820 760 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5218
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5770
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5770
NotI GC'GGCC_GC
Cuts at: 3217 3217
Size: 5770
NspI r_CATG'y
Cuts at: 1132 1229 2628 3128 4673 1132
Size: 97 1399 500 1545 2229
Fragments arranged by size:
2229 1545 1399 500 97
PasI CC'CwG_GG
Cuts at: 1348 2650 1348
Size: 1302 4468
PciI A'CATG_T
Cuts at: 4669 4669
Size: 5770
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5770
PfoI T'CCnGG_A
Cuts at: 2886 2886
Size: 5770
PmeI GTTT'AAAC
Cuts at: 3074 3074
Size: 5770
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3839 3871 5352 5384 3839
Size: 32 1481 32 4225
Fragments arranged by size:
4225 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5770
PsiI TTA'TAA
Cuts at: 3340 3340
Size: 5770
PstI C_TGCA'G
Cuts at: 830 2718 3122 830
Size: 1888 404 3478
Fragments arranged by size:
3478 1888 404
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5770
PvuII CAG'CTG
Cuts at: 2426 2426
Size: 5770
SalI G'TCGA_C
Cuts at: 1936 3112 1936
Size: 1176 4594
SapI GCTCTTCn'nnn_
Cuts at: 2437 2437
Size: 5770
SbfI CC_TGCA'GG
Cuts at: 3122 3122
Size: 5770
ScaI AGT'ACT
Cuts at: 4018 5730 4018
Size: 1712 4058
SfcI C'TryA_G
Cuts at: 826 1045 2714 3118 4253 4934 5125 826
Size: 219 1669 404 1135 681 191 1471
Fragments arranged by size:
1669 1471 1135 681 404 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5770
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5770
SmaI CCC'GGG
Cuts at: 1872 3097 1872
Size: 1225 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2722 2757 3874 4775 5037 820
Size: 197 926 779 35 1117 901 262 1553
Fragments arranged by size:
1553 1117 926 901 779 262 197 35
SnaBI TAC'GTA
Cuts at: 493 3067 3561 493
Size: 2574 494 2702
Fragments arranged by size:
2702 2574 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5770
SphI G_CATG'C
Cuts at: 1132 3128 1132
Size: 1996 3774
SspI AAT'ATT
Cuts at: 5 52 3694 5
Size: 47 3642 2081
Fragments arranged by size:
3642 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5770
StyI C'CwwG_G
Cuts at: 513 1065 2577 2586 3209 513
Size: 552 1512 9 623 3074
Fragments arranged by size:
3074 1512 623 552 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2150 3797 3814 3967 4152 4597 325
Size: 1255 570 1647 17 153 185 445 1498
Fragments arranged by size:
1647 1498 1255 570 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2319 2670 4016
Size: 274 80 33 51 1443 342 351 1346
Cuts at: 4016 5728 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1346 351 342 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5730 5762 5730
Size: 32 5738
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5770
XbaI T'CTAG_A
Cuts at: 2344 3106 2344
Size: 762 5008
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2657 2657
Size: 5770
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5770
XmaI C'CCGG_G
Cuts at: 1870 3095 1870
Size: 1225 4545
XmnI GAAnn'nnTTC
Cuts at: 3899 3899
Size: 5770
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3578 276
Size: 53 83 186 936 2044 2468
Fragments arranged by size:
2468 2044 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI