(Circular) (Six-base) MAPSORT of: pFN21ASDA1322.seq Check: 8967 from: 1 to: 6808 pFN21ASDA1322 6808 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4618 278 Size: 53 83 186 936 3082 2468 Fragments arranged by size: 3082 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2086 4151 1937 Size: 149 2065 4594 Fragments arranged by size: 4594 2065 149 Acc65I G'GTAC_C Cuts at: 4129 4129 Size: 6808 AclI AA'CG_TT Cuts at: 1563 4935 5308 1563 Size: 3372 373 3063 Fragments arranged by size: 3372 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2087 3027 3378 3911 4871 6255 6523 1632 Size: 455 940 351 533 960 1384 268 1917 Fragments arranged by size: 1917 1384 960 940 533 455 351 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6611 AflIII A'CryG_T Cuts at: 1203 2116 3486 3837 5707 6684 1203 Size: 913 1370 351 1870 977 1327 Fragments arranged by size: 1870 1370 1327 977 913 351 AgeI A'CCGG_T Cuts at: 5639 5639 Size: 6808 AhdI GACnn_n'nnGTC Cuts at: 5537 5537 Size: 6808 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6808 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6808 AlwNI CAG_nnn'CTG Cuts at: 2110 3946 4042 4551 6123 2110 Size: 1836 96 509 1572 2795 Fragments arranged by size: 2795 1836 1572 509 96 ApaI G_GGCC'C Cuts at: 2166 2166 Size: 6808 ApaLI G'TGCA_C Cuts at: 4865 6021 4865 Size: 1156 5652 ApoI r'AATT_y Cuts at: 1493 3712 4117 4343 4495 6604 1493 Size: 2219 405 226 152 2109 1697 Fragments arranged by size: 2219 2109 1697 405 226 152 AseI AT'TA_AT Cuts at: 160 1032 5362 160 Size: 872 4330 1606 Fragments arranged by size: 4330 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6808 AvaI C'yCGr_G Cuts at: 1870 1943 2218 2312 2969 3315 3959 4133 1870 Size: 73 275 94 657 346 644 174 4545 Fragments arranged by size: 4545 657 644 346 275 174 94 73 BamHI G'GATC_C Cuts at: 4138 4505 4138 Size: 367 6441 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2131 4129 5584 618 Size: 325 217 627 344 1998 1455 1842 Fragments arranged by size: 1998 1842 1455 627 344 325 217 BanII G_rGCy'C Cuts at: 1391 2166 2642 3297 4036 4127 1391 Size: 775 476 655 739 91 4072 Fragments arranged by size: 4072 775 739 655 476 91 BbeI G_GCGC'C Cuts at: 2135 2135 Size: 6808 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2196 2880 2893 928 Size: 971 297 684 13 4843 Fragments arranged by size: 4843 971 684 297 13 BbvCI CC'TCA_GC Cuts at: 2691 2691 Size: 6808 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3991 4025 4999 5033 1903 Size: 34 2054 34 974 34 3678 Fragments arranged by size: 3678 2054 974 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4700 5910 4700 Size: 1210 5598 BclI T'GATC_A Cuts at: 1548 1548 Size: 6808 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5419 136 Size: 107 122 71 631 605 3747 1525 Fragments arranged by size: 3747 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3162 6803 3162 Size: 3641 3167 BlpI GC'TnA_GC Cuts at: 3259 4225 3259 Size: 966 5842 Bme1580I G_kGCm'C Cuts at: 1790 2166 4869 6025 1790 Size: 376 2703 1156 2573 Fragments arranged by size: 2703 2573 1156 376 BmgBI CAC'GTC Cuts at: 1318 2119 3838 1318 Size: 801 1719 4288 Fragments arranged by size: 4288 1719 801 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3184 3934 4539 Size: 416 53 83 399 24 1767 750 605 Cuts at: 4539 5497 442 Size: 958 1753 Fragments arranged by size: 1767 1753 958 750 605 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6808 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2189 2255 2563 2618 3274 3431 3741 5468 Size: 950 66 308 55 656 157 310 1727 Cuts at: 5468 1239 Size: 2579 Fragments arranged by size: 2579 1727 950 656 310 308 157 66 55 Bpu10I CC'TnA_GC Cuts at: 2691 3341 4035 2691 Size: 650 694 5464 Fragments arranged by size: 5464 694 650 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2111 2656 4933 5798 6096 2111 Size: 545 2277 865 298 2823 Fragments arranged by size: 2823 2277 865 545 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5471 882 Size: 617 3972 2219 Fragments arranged by size: 3972 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2278 4105 4599 493 Size: 711 1074 1827 494 2702 Fragments arranged by size: 2702 1827 1074 711 494 BsaBI GATnn'nnATC Cuts at: 6802 6802 Size: 6808 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2132 4615 4997 5632 Size: 53 83 186 936 599 2483 382 635 Cuts at: 5632 275 Size: 1451 Fragments arranged by size: 2483 1451 936 635 599 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5241 5639 5913 6060 6471 5241 Size: 398 274 147 411 5578 Fragments arranged by size: 5578 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6583 Fragments arranged by size: 6583 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2296 2708 3992 1186 Size: 1110 412 1284 4002 Fragments arranged by size: 4002 1284 1110 412 BseYI C'CCAG_C Cuts at: 2992 3081 6011 6507 2992 Size: 89 2930 496 3293 Fragments arranged by size: 3293 2930 496 89 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6235 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4258 5019 6047 1540 Size: 275 186 2257 761 1028 2301 Fragments arranged by size: 2301 2257 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2642 3147 3194 3297 4869 4954 6025 2642 Size: 505 47 103 1572 85 1071 3425 Fragments arranged by size: 3425 1572 1071 505 103 85 47 BsmI GAATG_Cn' Cuts at: 2839 4319 4412 2839 Size: 1480 93 5235 Fragments arranged by size: 5235 1480 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2149 2149 Size: 6808 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2166 2642 3147 3194 3297 4036 4127 Size: 399 376 476 505 47 103 739 91 Cuts at: 4127 4869 4954 6025 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 739 505 476 399 376 103 91 85 47 BspEI T'CCGG_A Cuts at: 6471 6471 Size: 6808 BspHI T'CATG_A Cuts at: 2683 3481 4697 6427 2683 Size: 798 1216 1730 3064 Fragments arranged by size: 3064 1730 1216 798 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2118 3975 4163 844 Size: 1274 1857 188 3489 Fragments arranged by size: 3489 1857 1274 188 BsrBI CCG'CTC Cuts at: 1394 1823 2317 2380 4695 1394 Size: 429 494 63 2315 3507 Fragments arranged by size: 3507 2315 494 429 63 BsrDI GCAATG_nn' Cuts at: 65 1248 2548 4049 5303 5477 65 Size: 1183 1300 1501 1254 174 1396 Fragments arranged by size: 1501 1396 1300 1254 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2193 3522 5452 5639 1700 Size: 110 383 1329 1930 187 2869 Fragments arranged by size: 2869 1930 1329 383 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6808 BssHII G'CGCG_C Cuts at: 1925 2337 1925 Size: 412 6396 BssSI C'ACGA_G Cuts at: 2569 3290 4868 5880 2569 Size: 721 1578 1012 3497 Fragments arranged by size: 3497 1578 1012 721 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6808 BstBI TT'CG_AA Cuts at: 2007 5612 2007 Size: 3605 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6808 BstXI CCAn_nnnn'nTGG Cuts at: 3470 3470 Size: 6808 BstYI r'GATC_y Cuts at: 1972 3162 3780 4138 4505 4890 4907 6359 6445 Size: 1190 618 358 367 385 17 1452 86 Cuts at: 6445 6457 6803 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1190 618 385 367 358 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2211 2656 3661 513 Size: 552 1146 445 1005 3660 Fragments arranged by size: 3660 1146 1005 552 445 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2490 3864 3979 487 Size: 683 56 1264 1374 115 3316 Fragments arranged by size: 3316 1374 1264 683 115 56 BtsI GCAGTG_nn' Cuts at: 3573 4324 5118 5138 3573 Size: 751 794 20 5243 Fragments arranged by size: 5243 794 751 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6808 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6773 DraI TTT'AAA Cuts at: 4112 4112 Size: 6808 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2518 2564 5815 809 Size: 507 1202 46 3251 1802 Fragments arranged by size: 3251 1802 1202 507 46 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3524 4255 5144 Size: 54 1425 50 275 18 1694 731 889 Cuts at: 5144 6773 8 Size: 1629 43 Fragments arranged by size: 1694 1629 1425 889 731 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4255 1537 Size: 275 2443 4090 Fragments arranged by size: 4090 2443 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3852 4738 1353 Size: 2499 886 3423 Fragments arranged by size: 3423 2499 886 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5395 5769 5915 387 Size: 1064 138 3806 374 146 1280 Fragments arranged by size: 3806 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2640 3295 2640 Size: 655 6153 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2087 2189 2255 2563 2618 3027 3274 Size: 393 455 102 66 308 55 409 247 Cuts at: 3274 3378 3431 3741 3911 4871 5468 6255 6523 Size: 104 53 310 170 960 597 787 268 Cuts at: 6523 1239 Size: 1524 Fragments arranged by size: 1524 960 787 597 455 409 393 310 308 268 247 170 104 102 66 55 53 EcoO109I rG'GnC_Cy Cuts at: 3964 3964 Size: 6808 EcoRI G'AATT_C Cuts at: 4117 4495 4117 Size: 378 6430 EcoRV GAT'ATC Cuts at: 3763 3763 Size: 6808 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3940 3940 Size: 6808 FspI TGC'GCA Cuts at: 2396 3732 5314 2396 Size: 1336 1582 3890 Fragments arranged by size: 3890 1582 1336 HaeII r_GCGC'y Cuts at: 1678 2135 2334 2822 3510 5955 1678 Size: 457 199 488 688 2445 2531 Fragments arranged by size: 2531 2445 688 488 457 199 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2468 2500 2914 2946 3102 Size: 32 135 32 896 32 414 32 156 Cuts at: 3102 3134 3234 3266 5449 5481 5523 5555 1373 Size: 32 100 32 2183 32 42 32 2626 Fragments arranged by size: 2626 2183 896 414 156 135 100 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2087 3675 4152 4398 669 Size: 988 281 149 1588 477 246 3079 Fragments arranged by size: 3079 1588 988 477 281 246 149 HindIII A'AGCT_T Cuts at: 748 748 Size: 6808 HpaI GTT'AAC Cuts at: 4398 4398 Size: 6808 KasI G'GCGC_C Cuts at: 2131 2131 Size: 6808 KpnI G_GTAC'C Cuts at: 4133 4133 Size: 6808 MfeI C'AATT_G Cuts at: 4407 4407 Size: 6808 MluI A'CGCG_T Cuts at: 6684 6684 Size: 6808 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2319 2325 4120 5922 6106 1305 Size: 1014 6 1795 1802 184 2007 Fragments arranged by size: 2007 1802 1795 1014 184 6 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4986 Fragments arranged by size: 4986 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3932 4766 5125 5284 518 Size: 648 2766 834 359 159 2042 Fragments arranged by size: 2766 2042 834 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3005 3100 4224 4901 6049 6294 1606 Size: 1399 95 1124 677 1148 245 2120 Fragments arranged by size: 2120 1399 1148 1124 677 245 95 NarI GG'CG_CC Cuts at: 2132 2132 Size: 6808 NcoI C'CATG_G Cuts at: 513 1065 3661 513 Size: 552 2596 3660 Fragments arranged by size: 3660 2596 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6808 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6808 NotI GC'GGCC_GC Cuts at: 4255 4255 Size: 6808 NruI TCG'CGA Cuts at: 3849 3849 Size: 6808 NspI r_CATG'y Cuts at: 1132 1229 3490 3553 4166 5711 1132 Size: 97 2261 63 613 1545 2229 Fragments arranged by size: 2261 2229 1545 613 97 63 PasI CC'CwG_GG Cuts at: 1348 3880 1348 Size: 2532 4276 PciI A'CATG_T Cuts at: 3486 5707 3486 Size: 2221 4587 PflMI CCAn_nnn'nTGG Cuts at: 1108 2212 1108 Size: 1104 5704 PmeI GTTT'AAAC Cuts at: 4112 4112 Size: 6808 PmlI CAC'GTG Cuts at: 2278 2278 Size: 6808 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3851 3883 4877 4909 6390 6422 3851 Size: 32 994 32 1481 32 4237 Fragments arranged by size: 4237 1481 994 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3964 3964 Size: 6808 PshAI GACnn'nnGTC Cuts at: 1613 3306 1613 Size: 1693 5115 PsiI TTA'TAA Cuts at: 4378 4378 Size: 6808 PspOMI G'GGCC_C Cuts at: 2162 2162 Size: 6808 PspXI vC'TCGA_Gb Cuts at: 2969 2969 Size: 6808 PstI C_TGCA'G Cuts at: 830 2267 2495 3595 4160 830 Size: 1437 228 1100 565 3478 Fragments arranged by size: 3478 1437 1100 565 228 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6808 PvuII CAG'CTG Cuts at: 3005 3100 3005 Size: 95 6713 SacI G_AGCT'C Cuts at: 2642 3297 2642 Size: 655 6153 SalI G'TCGA_C Cuts at: 1936 2085 4150 1936 Size: 149 2065 4594 Fragments arranged by size: 4594 2065 149 SbfI CC_TGCA'GG Cuts at: 4160 4160 Size: 6808 ScaI AGT'ACT Cuts at: 5056 6768 5056 Size: 1712 5096 SfcI C'TryA_G Cuts at: 826 1045 2263 2443 2491 3591 4156 5291 5972 Size: 219 1218 180 48 1100 565 1135 681 Cuts at: 5972 6163 826 Size: 191 1471 Fragments arranged by size: 1471 1218 1135 1100 681 565 219 191 180 48 SfoI GGC'GCC Cuts at: 2133 2133 Size: 6808 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6808 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6808 SmaI CCC'GGG Cuts at: 1872 4135 1872 Size: 2263 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2126 2635 2969 4912 5813 6075 Size: 197 926 183 509 334 1943 901 262 Cuts at: 6075 820 Size: 1553 Fragments arranged by size: 1943 1553 926 901 509 334 262 197 183 SnaBI TAC'GTA Cuts at: 493 4105 4599 493 Size: 3612 494 2702 Fragments arranged by size: 3612 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6808 SphI G_CATG'C Cuts at: 1132 4166 1132 Size: 3034 3774 SspI AAT'ATT Cuts at: 5 52 2715 4732 5 Size: 47 2663 2017 2081 Fragments arranged by size: 2663 2081 2017 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6808 StyI C'CwwG_G Cuts at: 513 1065 3321 3661 4247 513 Size: 552 2256 340 586 3074 Fragments arranged by size: 3074 2256 586 552 340 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3302 4835 4852 5005 5190 5635 325 Size: 1255 1722 1533 17 153 185 445 1498 Fragments arranged by size: 1722 1533 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5054 6766 96 Size: 274 80 33 51 1443 3077 1712 138 Fragments arranged by size: 3077 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3126 3158 3170 3202 6768 6800 3126 Size: 32 12 32 3566 32 3134 Fragments arranged by size: 3566 3134 32 32 32 12 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6808 XbaI T'CTAG_A Cuts at: 3194 3434 4144 3194 Size: 240 710 5858 Fragments arranged by size: 5858 710 240 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2593 2593 Size: 6808 XhoI C'TCGA_G Cuts at: 1943 2969 1943 Size: 1026 5782 XmaI C'CCGG_G Cuts at: 1870 4133 1870 Size: 2263 4545 XmnI GAAnn'nnTTC Cuts at: 3896 4937 3896 Size: 1041 5767 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4616 276 Size: 53 83 186 936 3082 2468 Fragments arranged by size: 3082 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoNI FseI FspAI NaeI NgoMIV NsiI PacI PfoI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI