Restriction map for
pFN21ASDA1293
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# NO CUT ENZYMES ( 137 enzymes) #
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AatII AccI AccIII AclI AcyI
AflII AflIII AgeI AhdI AocI
Aor51HI ApaI AscI AseI AsnI
Asp700I AspEI AspI AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BciVI BclI BfaI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BspDI BspEI BspHI
BspLU11I BspMI BsrBI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT22I FbaI
FseI FspI HgaI Hin1I HincII
HindII HpaI KasI KspI MaeI
MaeII MamI MfeI MluI MluNI
MroI MscI MseI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tru9I Tth111I
XbaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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Acc65I 113 GGTACC G^GTACC
AccII 1232 CGCG CG^CG
AcsI 213 GAATTC [GA]^AATT[CT]
Alw44I 106 GTGCAC G^TGCAC
ApaLI 106 GTGCAC G^TGCAC
ApoI 213 GAATTC [GA]^AATT[CT]
Asp718I 113 GGTACC G^GTACC
BcgI 175 CGAGCCCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaI 206 GAGACC GGTCTC(1/5)
BsmI 397 GCATTC GAATGC(1/-1)
Bsp1407I 839 TGTACA T^GTACA
BsrDI 740 GCAATG GCAATG(2/0)
BsrFI 961 ACCGGC [GA]^CCGG[CT]
BsrGI 839 TGTACA T^GTACA
BssHII 1231 GCGCGC G^CGCGC
BstUI 1232 CGCG CG^CG
BstXI 836 CCATGTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 961 ACCGGC [GA]^CCGG[CT]
EarI 673 CTCTTC CTCTTC(1/4)
EcoRI 213 GAATTC G^AATTC
EcoT14I 418 CCAAGG C^C[AT][AT]GG
HaeII 1104 GGCGCT [GA]GCGC^[CT]
HindIII 485 AAGCTT A^AGCTT
HphI 1098 GGTGA GGTGA(8/7)
KpnI 113 GGTACC GGTAC^C
Ksp632I 673 CTCTTC CTCTTC(1/4)
MvnI 1232 CGCG CG^CG
NspI 607 GCATGT [GA]CATG^[CT]
PaeR7I 173 CTCGAG C^TCGAG
PflMI 1167 CCACATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 999 CAGCTG CAG^CTG
SacI 670 GAGCTC GAGCT^C
SapI 672 GCTCTTC GCTCTTC(1/4)
SspBI 839 TGTACA T^GTACA
StyI 418 CCAAGG C^C[AT][AT]GG
Tsp45I 958 GTGAC ^GT[GC]AC
Van91I 1167 CCACATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 173 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BanII 176 GAGCCC G[GA]GC[CT]^C
670 GAGCTC
BbsI 1153 GAAGAC GAAGAC(2/6)
34 GTCTTC
BglII 22 AGATCT A^GATCT
542 AGATCT
BpuAI 1153 GAAGAC GAAGAC(2/6)
34 GTCTTC
BsmAI 206 GAGAC GTCTC(1/5)
440 GTCTC
DraII 338 AGGTCCT [GA]G^G[ACGT]CC[CT]
947 GGGACCC
EcoO109I 338 AGGTCCT [GA]G^G[ACGT]CC[CT]
947 GGGACCC
MaeIII 958 GTGAC ^GT[ACGT]AC
1160 GTTAC
MslI 837 CATGTACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
871 CACGCCAATG
PpuMI 338 AGGTCCT [GA]G^G[AT]CC[CT]
947 GGGACCC
PstI 679 CTGCAG CTGCA^G
830 CTGCAG
SmaI 65 CCCGGG CCC^GGG
1094 CCCGGG
SmlI 173 CTCGAG C^T[CT][GA]AG
331 CTCAAG
TaqI 174 TCGA T^CGA
668 TCGA
TthHB8I 174 TCGA T^CGA
668 TCGA
XcmI 46 CCAGCCCCCTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
118 CCAGTCCTGGCCTGG
XmaCI 65 CCCGGG C^CCGGG
1094 CCCGGG
XmaI 65 CCCGGG C^CCGGG
1094 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BanI BbvI
BcnI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI MboI MboII MflI
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TfiI TseI Tsp509I TspRI XhoII