(Circular) (Six-base) MAPSORT of: pFN21ASDA1293.seq Check: 5520 from: 1 to: 5986 pFN21ASDA1293 5986 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3796 278 Size: 53 83 186 936 2260 2468 Fragments arranged by size: 2468 2260 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3329 1937 Size: 1392 4594 Acc65I G'GTAC_C Cuts at: 2136 3307 2136 Size: 1171 4815 AclI AA'CG_TT Cuts at: 1563 4113 4486 1563 Size: 2550 373 3063 Fragments arranged by size: 3063 2550 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2669 3098 4049 5433 5701 1632 Size: 1037 429 951 1384 268 1917 Fragments arranged by size: 1917 1384 1037 951 429 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5789 AflIII A'CryG_T Cuts at: 1203 4885 5862 1203 Size: 3682 977 1327 Fragments arranged by size: 3682 1327 977 AgeI A'CCGG_T Cuts at: 4817 4817 Size: 5986 AhdI GACnn_n'nnGTC Cuts at: 4715 4715 Size: 5986 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5986 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2916 1184 Size: 1732 4254 AlwNI CAG_nnn'CTG Cuts at: 2737 3043 3076 3729 5301 2737 Size: 306 33 653 1572 3422 Fragments arranged by size: 3422 1572 653 306 33 ApaLI G'TGCA_C Cuts at: 2129 4043 5199 2129 Size: 1914 1156 2916 Fragments arranged by size: 2916 1914 1156 ApoI r'AATT_y Cuts at: 1493 2236 3295 3521 3673 5782 1493 Size: 743 1059 226 152 2109 1697 Fragments arranged by size: 2109 1697 1059 743 226 152 AseI AT'TA_AT Cuts at: 160 1032 4540 160 Size: 872 3508 1606 Fragments arranged by size: 3508 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5986 AvaI C'yCGr_G Cuts at: 1870 1943 2088 2196 3117 3311 1870 Size: 73 145 108 921 194 4545 Fragments arranged by size: 4545 921 194 145 108 73 BamHI G'GATC_C Cuts at: 3316 3683 3316 Size: 367 5619 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2083 2136 2172 2322 3307 Size: 325 217 627 296 53 36 150 985 Cuts at: 3307 4762 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 985 627 325 296 217 150 53 36 BanII G_rGCy'C Cuts at: 1391 2203 2697 3305 1391 Size: 812 494 608 4072 Fragments arranged by size: 4072 812 608 494 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2050 3183 928 Size: 971 151 1133 3731 Fragments arranged by size: 3731 1133 971 151 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2187 2221 4177 4211 1903 Size: 34 250 34 1956 34 3678 Fragments arranged by size: 3678 1956 250 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3878 5088 3878 Size: 1210 4776 BclI T'GATC_A Cuts at: 1548 1548 Size: 5986 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4597 136 Size: 107 122 71 631 605 2925 1525 Fragments arranged by size: 2925 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2045 2565 5981 2045 Size: 520 3416 2050 Fragments arranged by size: 3416 2050 520 BlpI GC'TnA_GC Cuts at: 3403 3403 Size: 5986 Bme1580I G_kGCm'C Cuts at: 1790 2086 2133 2164 2325 4047 5203 1790 Size: 296 47 31 161 1722 1156 2573 Fragments arranged by size: 2573 1722 1156 296 161 47 31 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5986 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2089 2135 2970 Size: 416 53 83 399 24 672 46 835 Cuts at: 2970 3717 4675 442 Size: 747 958 1753 Fragments arranged by size: 1753 958 835 747 672 416 399 83 53 46 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5986 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2387 2450 2939 3042 4646 1239 Size: 1148 63 489 103 1604 2579 Fragments arranged by size: 2579 1604 1148 489 103 63 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2339 4111 4976 5274 2339 Size: 1772 865 298 3051 Fragments arranged by size: 3051 1772 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2223 4649 882 Size: 617 724 2426 2219 Fragments arranged by size: 2426 2219 724 617 BsaAI yAC'GTr Cuts at: 493 1204 3283 3777 493 Size: 711 2079 494 2702 Fragments arranged by size: 2702 2079 711 494 BsaBI GATnn'nnATC Cuts at: 5980 5980 Size: 5986 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3793 4175 4810 275 Size: 53 83 186 936 2260 382 635 1451 Fragments arranged by size: 2260 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4419 4817 5091 5238 5649 4419 Size: 398 274 147 411 4756 Fragments arranged by size: 4756 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5761 Fragments arranged by size: 5761 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2200 2735 3152 1186 Size: 1014 535 417 4020 Fragments arranged by size: 4020 1014 535 417 BseYI C'CCAG_C Cuts at: 2101 3232 5189 5685 2101 Size: 1131 1957 496 2402 Fragments arranged by size: 2402 1957 1131 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5413 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3436 4197 5225 1540 Size: 275 186 1435 761 1028 2301 Fragments arranged by size: 2301 1435 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2133 2697 2895 4047 4132 5203 2133 Size: 564 198 1152 85 1071 2916 Fragments arranged by size: 2916 1152 1071 564 198 85 BsmI GAATG_Cn' Cuts at: 2420 3497 3590 2420 Size: 1077 93 4816 Fragments arranged by size: 4816 1077 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2086 2133 2164 2203 2325 2697 2895 Size: 399 296 47 31 39 122 372 198 Cuts at: 2895 3305 4047 4132 5203 1391 Size: 410 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 410 399 372 296 198 122 85 47 39 31 BspEI T'CCGG_A Cuts at: 5649 5649 Size: 5986 BspHI T'CATG_A Cuts at: 3875 5605 3875 Size: 1730 4256 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3341 844 Size: 2497 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3873 1394 Size: 429 2050 3507 Fragments arranged by size: 3507 2050 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2770 4481 4655 65 Size: 1183 1522 1711 174 1396 Fragments arranged by size: 1711 1522 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2984 4630 4817 1700 Size: 110 1174 1646 187 2869 Fragments arranged by size: 2869 1646 1174 187 110 BsrGI T'GTAC_A Cuts at: 96 2862 96 Size: 2766 3220 BssHII G'CGCG_C Cuts at: 1925 3254 1925 Size: 1329 4657 BssSI C'ACGA_G Cuts at: 4046 5058 4046 Size: 1012 4974 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5986 BstBI TT'CG_AA Cuts at: 2007 4790 2007 Size: 2783 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5986 BstXI CCAn_nnnn'nTGG Cuts at: 2866 2866 Size: 5986 BstYI r'GATC_y Cuts at: 1972 2045 2565 2770 2910 3072 3145 3316 3683 Size: 73 520 205 140 162 73 171 367 Cuts at: 3683 4068 4085 5537 5623 5635 5981 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 520 385 367 346 205 171 162 140 86 73 73 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5434 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5247 Fragments arranged by size: 5247 683 56 BtsI GCAGTG_nn' Cuts at: 3162 3502 4296 4316 3162 Size: 340 794 20 4832 Fragments arranged by size: 4832 794 340 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5986 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5951 DraI TTT'AAA Cuts at: 3290 3290 Size: 5986 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4993 809 Size: 507 3677 1802 Fragments arranged by size: 3677 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3433 4322 5951 Size: 54 1425 50 275 18 1603 889 1629 Cuts at: 5951 8 Size: 43 Fragments arranged by size: 1629 1603 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3433 1537 Size: 275 1621 4090 Fragments arranged by size: 4090 1621 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2702 3916 1353 Size: 1349 1214 3423 Fragments arranged by size: 3423 1349 1214 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4573 4947 5093 387 Size: 1064 138 2984 374 146 1280 Fragments arranged by size: 2984 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2695 2695 Size: 5986 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2387 2450 2669 2939 3042 3098 4049 Size: 393 755 63 219 270 103 56 951 Cuts at: 4049 4646 5433 5701 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 951 787 755 597 393 270 268 219 103 63 56 EcoO109I rG'GnC_Cy Cuts at: 2362 2971 2362 Size: 609 5377 EcoRI G'AATT_C Cuts at: 2236 3295 3673 2236 Size: 1059 378 4549 Fragments arranged by size: 4549 1059 378 FspI TGC'GCA Cuts at: 4492 4492 Size: 5986 HaeII r_GCGC'y Cuts at: 1678 3131 5133 1678 Size: 1453 2002 2531 Fragments arranged by size: 2531 2002 1453 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2698 2730 4627 4659 4701 Size: 32 135 32 1126 32 1897 32 42 Cuts at: 4701 4733 1373 Size: 32 2626 Fragments arranged by size: 2626 1897 1126 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3330 3576 669 Size: 988 281 1392 246 3079 Fragments arranged by size: 3079 1392 988 281 246 HindIII A'AGCT_T Cuts at: 748 2508 748 Size: 1760 4226 HpaI GTT'AAC Cuts at: 3576 3576 Size: 5986 KpnI G_GTAC'C Cuts at: 2140 3311 2140 Size: 1171 4815 MfeI C'AATT_G Cuts at: 3585 3585 Size: 5986 MluI A'CGCG_T Cuts at: 5862 5862 Size: 5986 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5100 5284 1305 Size: 3795 184 2007 Fragments arranged by size: 3795 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4164 Fragments arranged by size: 4164 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2864 2898 3944 4303 4462 518 Size: 648 1698 34 1046 359 159 2042 Fragments arranged by size: 2042 1698 1046 648 359 159 34 MspA1I CmG'CkG Cuts at: 1606 2037 2166 2470 3024 3402 4079 5227 5472 Size: 431 129 304 554 378 677 1148 245 Cuts at: 5472 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 554 431 378 304 245 129 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5434 NdeI CA'TA_TG Cuts at: 387 387 Size: 5986 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5986 NotI GC'GGCC_GC Cuts at: 3433 3433 Size: 5986 NspI r_CATG'y Cuts at: 1132 1229 2634 3344 4889 1132 Size: 97 1405 710 1545 2229 Fragments arranged by size: 2229 1545 1405 710 97 PasI CC'CwG_GG Cuts at: 1348 2758 1348 Size: 1410 4576 PciI A'CATG_T Cuts at: 4885 4885 Size: 5986 PflMI CCAn_nnn'nTGG Cuts at: 1108 3196 1108 Size: 2088 3898 PfoI T'CCnGG_A Cuts at: 2364 2424 2622 3006 2364 Size: 60 198 384 5344 Fragments arranged by size: 5344 384 198 60 PmeI GTTT'AAAC Cuts at: 3290 3290 Size: 5986 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4055 4087 5568 5600 4055 Size: 32 1481 32 4441 Fragments arranged by size: 4441 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2362 2971 2362 Size: 609 5377 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5986 PsiI TTA'TAA Cuts at: 3556 3556 Size: 5986 PspXI vC'TCGA_Gb Cuts at: 2196 2196 Size: 5986 PstI C_TGCA'G Cuts at: 830 2706 2857 3338 830 Size: 1876 151 481 3478 Fragments arranged by size: 3478 1876 481 151 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5986 PvuII CAG'CTG Cuts at: 3024 3024 Size: 5986 SacI G_AGCT'C Cuts at: 2697 2697 Size: 5986 SalI G'TCGA_C Cuts at: 1936 3328 1936 Size: 1392 4594 SanDI GG'GwC_CC Cuts at: 2971 2971 Size: 5986 SapI GCTCTTCn'nnn_ Cuts at: 2702 2702 Size: 5986 SbfI CC_TGCA'GG Cuts at: 3338 3338 Size: 5986 ScaI AGT'ACT Cuts at: 4234 5946 4234 Size: 1712 4274 SfcI C'TryA_G Cuts at: 826 1045 2702 2847 2853 3334 4469 5150 5341 Size: 219 1657 145 6 481 1135 681 191 Cuts at: 5341 826 Size: 1471 Fragments arranged by size: 1657 1471 1135 681 481 219 191 145 6 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5986 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5986 SmaI CCC'GGG Cuts at: 1872 2090 3119 3313 1872 Size: 218 1029 194 4545 Fragments arranged by size: 4545 1029 218 194 SmlI C'TyrA_G Cuts at: 820 1017 1943 2196 2354 4090 4991 5253 820 Size: 197 926 253 158 1736 901 262 1553 Fragments arranged by size: 1736 1553 926 901 262 253 197 158 SnaBI TAC'GTA Cuts at: 493 3283 3777 493 Size: 2790 494 2702 Fragments arranged by size: 2790 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5986 SphI G_CATG'C Cuts at: 1132 3344 1132 Size: 2212 3774 SspI AAT'ATT Cuts at: 5 52 3910 5 Size: 47 3858 2081 Fragments arranged by size: 3858 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5986 StyI C'CwwG_G Cuts at: 513 1065 2441 3425 513 Size: 552 1376 984 3074 Fragments arranged by size: 3074 1376 984 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2340 4013 4030 4183 4368 4813 325 Size: 1255 760 1673 17 153 185 445 1498 Fragments arranged by size: 1673 1498 1255 760 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2370 2814 2862 Size: 274 80 33 51 1443 393 444 48 Cuts at: 2862 4232 5944 96 Size: 1370 1712 138 Fragments arranged by size: 1712 1443 1370 444 393 274 138 80 51 48 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5946 5978 5946 Size: 32 5954 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5986 XbaI T'CTAG_A Cuts at: 3322 3322 Size: 5986 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2076 2148 2076 Size: 72 5914 XhoI C'TCGA_G Cuts at: 1943 2196 1943 Size: 253 5733 XmaI C'CCGG_G Cuts at: 1870 2088 3117 3311 1870 Size: 218 1029 194 4545 Fragments arranged by size: 4545 1029 218 194 XmnI GAAnn'nnTTC Cuts at: 4115 4115 Size: 5986 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3794 276 Size: 53 83 186 936 2260 2468 Fragments arranged by size: 2468 2260 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI