Restriction map for
pFN21ASDA1293
(Linear) (Six-base) MAPSORT of: KIAA1293.seq Check: 7257 from: 1 to: 1257
KIAA1293.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
Acc65I G'GTAC_C
Cuts at: 0 113 1257
Size: 113 1144
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 646 1075 1257
Size: 646 429 182
Fragments arranged by size:
646 429 182
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 893 1257
Size: 893 364
AlwNI CAG_nnn'CTG
Cuts at: 0 714 1020 1053 1257
Size: 714 306 33 204
Fragments arranged by size:
714 306 204 33
ApaLI G'TGCA_C
Cuts at: 0 106 1257
Size: 106 1151
ApoI r'AATT_y
Cuts at: 0 213 1257
Size: 213 1044
AvaI C'yCGr_G
Cuts at: 0 65 173 1094 1257
Size: 65 108 921 163
Fragments arranged by size:
921 163 108 65
BanI G'GyrC_C
Cuts at: 0 60 113 149 299 1257
Size: 60 53 36 150 958
Fragments arranged by size:
958 150 60 53 36
BanII G_rGCy'C
Cuts at: 0 180 674 1257
Size: 180 494 583
Fragments arranged by size:
583 494 180
BbsI GAAGACnn'nnnn_
Cuts at: 0 27 1160 1257
Size: 27 1133 97
Fragments arranged by size:
1133 97 27
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 164 198 1257
Size: 164 34 1059
Fragments arranged by size:
1059 164 34
BglII A'GATC_T
Cuts at: 0 22 542 1257
Size: 22 520 715
Fragments arranged by size:
715 520 22
Bme1580I G_kGCm'C
Cuts at: 0 63 110 141 302 1257
Size: 63 47 31 161 955
Fragments arranged by size:
955 161 63 47 31
BmrI ACTGGGnnnn_n'
Cuts at: 0 66 112 947 1257
Size: 66 46 835 310
Fragments arranged by size:
835 310 66 46
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 427 916 1019 1257
Size: 364 63 489 103 238
Fragments arranged by size:
489 364 238 103 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 1257
Size: 316 941
BsaI GGTCTCn'nnnn_
Cuts at: 0 200 1257
Size: 200 1057
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 177 712 1129 1257
Size: 177 535 417 128
Fragments arranged by size:
535 417 177 128
BseYI C'CCAG_C
Cuts at: 0 78 1209 1257
Size: 78 1131 48
Fragments arranged by size:
1131 78 48
BsiHKAI G_wGCw'C
Cuts at: 0 110 674 872 1257
Size: 110 564 198 385
Fragments arranged by size:
564 385 198 110
BsmI GAATG_Cn'
Cuts at: 0 397 1257
Size: 397 860
Bsp1286I G_dGCh'C
Cuts at: 0 63 110 141 180 302 674 872 1257
Size: 63 47 31 39 122 372 198 385
Fragments arranged by size:
385 372 198 122 63 47 39 31
BsrDI GCAATG_nn'
Cuts at: 0 747 1257
Size: 747 510
BsrFI r'CCGG_y
Cuts at: 0 961 1257
Size: 961 296
BsrGI T'GTAC_A
Cuts at: 0 839 1257
Size: 839 418
BssHII G'CGCG_C
Cuts at: 0 1231 1257
Size: 1231 26
BstXI CCAn_nnnn'nTGG
Cuts at: 0 843 1257
Size: 843 414
BstYI r'GATC_y
Cuts at: 0 22 542 747 887 1049 1122 1257
Size: 22 520 205 140 162 73 135
Fragments arranged by size:
520 205 162 140 135 73 22
BtsI GCAGTG_nn'
Cuts at: 0 1139 1257
Size: 1139 118
EarI CTCTTCn'nnn_
Cuts at: 0 679 1257
Size: 679 578
EcoICRI GAG'CTC
Cuts at: 0 672 1257
Size: 672 585
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 427 646 916 1019 1075 1257
Size: 364 63 219 270 103 56 182
Fragments arranged by size:
364 270 219 182 103 63 56
EcoO109I rG'GnC_Cy
Cuts at: 0 339 948 1257
Size: 339 609 309
Fragments arranged by size:
609 339 309
EcoRI G'AATT_C
Cuts at: 0 213 1257
Size: 213 1044
HaeII r_GCGC'y
Cuts at: 0 1108 1257
Size: 1108 149
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 675 707 1257
Size: 675 32 550
Fragments arranged by size:
675 550 32
HindIII A'AGCT_T
Cuts at: 0 485 1257
Size: 485 772
KpnI G_GTAC'C
Cuts at: 0 117 1257
Size: 117 1140
MslI CAynn'nnrTG
Cuts at: 0 841 875 1257
Size: 841 34 382
Fragments arranged by size:
841 382 34
MspA1I CmG'CkG
Cuts at: 0 14 143 447 1001 1257
Size: 14 129 304 554 256
Fragments arranged by size:
554 304 256 129 14
NspI r_CATG'y
Cuts at: 0 611 1257
Size: 611 646
PasI CC'CwG_GG
Cuts at: 0 735 1257
Size: 735 522
PflMI CCAn_nnn'nTGG
Cuts at: 0 1173 1257
Size: 1173 84
PfoI T'CCnGG_A
Cuts at: 0 341 401 599 983 1257
Size: 341 60 198 384 274
Fragments arranged by size:
384 341 274 198 60
PpuMI rG'GwC_Cy
Cuts at: 0 339 948 1257
Size: 339 609 309
Fragments arranged by size:
609 339 309
PspXI vC'TCGA_Gb
Cuts at: 0 173 1257
Size: 173 1084
PstI C_TGCA'G
Cuts at: 0 683 834 1257
Size: 683 151 423
Fragments arranged by size:
683 423 151
PvuII CAG'CTG
Cuts at: 0 1001 1257
Size: 1001 256
SacI G_AGCT'C
Cuts at: 0 674 1257
Size: 674 583
SanDI GG'GwC_CC
Cuts at: 0 948 1257
Size: 948 309
SapI GCTCTTCn'nnn_
Cuts at: 0 679 1257
Size: 679 578
SfcI C'TryA_G
Cuts at: 0 679 824 830 1257
Size: 679 145 6 427
Fragments arranged by size:
679 427 145 6
SmaI CCC'GGG
Cuts at: 0 67 1096 1257
Size: 67 1029 161
Fragments arranged by size:
1029 161 67
SmlI C'TyrA_G
Cuts at: 0 173 331 1257
Size: 173 158 926
Fragments arranged by size:
926 173 158
StyI C'CwwG_G
Cuts at: 0 418 1257
Size: 418 839
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 317 1257
Size: 317 940
TatI w'GTAC_w
Cuts at: 0 347 791 839 1257
Size: 347 444 48 418
Fragments arranged by size:
444 418 347 48
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 53 125 1257
Size: 53 72 1132
Fragments arranged by size:
1132 72 53
XhoI C'TCGA_G
Cuts at: 0 173 1257
Size: 173 1084
XmaI C'CCGG_G
Cuts at: 0 65 1094 1257
Size: 65 1029 163
Fragments arranged by size:
1029 163 65
Enzymes that do cut:
Acc65I AcuI AlfI AlwNI ApaLI ApoI AvaI BanI
BanII BbsI BcgI BglII Bme1580I BmrI BpmI BpuEI
BsaI BseRI BseYI BsiHKAI BsmI Bsp1286I BsrDI BsrFI
BsrGI BssHII BstXI BstYI BtsI EarI EcoICRI Eco57MI
EcoO109I EcoRI HaeII Hin4I HindIII KpnI MslI MspA1I
NspI PasI PflMI PfoI PpuMI PspXI PstI PvuII
SacI SanDI SapI SfcI SmaI SmlI StyI TaqII
TatI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BciVI BclI BfrBI BglI
BlpI BmgBI BmtI BplI Bpu10I BsaAI BsaBI BsaHI
BsaWI BsaXI BsgI BsiEI BsiWI BsmBI BspEI BspHI
BspMI BsrBI BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
BtgI BtgZI ClaI CspCI DraI DraIII DrdI EaeI
EagI EciI EcoNI EcoRV FalI FseI FspI FspAI
HincII HpaI KasI MfeI MluI MmeI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PmeI PmlI PpiI PshAI PsiI PspOMI
PsrI PvuI RsrII SacII SalI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StuI SwaI TstI Tth111I XbaI XmnI ZraI