(Circular) (Six-base) MAPSORT of: pFN21ASDA1284.seq Check: 5594 from: 1 to: 7555 pFN21ASDA1284 7555 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2779 2779 Size: 7555 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5365 278 Size: 53 83 186 936 3829 2468 Fragments arranged by size: 3829 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3557 3642 4310 4898 1937 Size: 1620 85 668 588 4594 Fragments arranged by size: 4594 1620 668 588 85 Acc65I G'GTAC_C Cuts at: 4876 4876 Size: 7555 AclI AA'CG_TT Cuts at: 1563 5682 6055 1563 Size: 4119 373 3063 Fragments arranged by size: 4119 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3045 3219 3335 3780 3798 5618 7002 7270 Size: 1413 174 116 445 18 1820 1384 268 Cuts at: 7270 1632 Size: 1917 Fragments arranged by size: 1917 1820 1413 1384 445 268 174 116 18 AfeI AGC'GCT Cuts at: 2402 2402 Size: 7555 AflII C'TTAA_G Cuts at: 820 1017 4289 820 Size: 197 3272 4086 Fragments arranged by size: 4086 3272 197 AflIII A'CryG_T Cuts at: 1203 6454 7431 1203 Size: 5251 977 1327 Fragments arranged by size: 5251 1327 977 AgeI A'CCGG_T Cuts at: 6386 6386 Size: 7555 AhdI GACnn_n'nnGTC Cuts at: 4401 6284 4401 Size: 1883 5672 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7555 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3417 3626 1184 Size: 2233 209 5113 Fragments arranged by size: 5113 2233 209 AlwNI CAG_nnn'CTG Cuts at: 4619 5298 6870 4619 Size: 679 1572 5304 Fragments arranged by size: 5304 1572 679 ApaLI G'TGCA_C Cuts at: 5612 6768 5612 Size: 1156 6399 ApoI r'AATT_y Cuts at: 1493 2370 3000 3141 3257 4522 4864 5090 5242 Size: 877 630 141 116 1265 342 226 152 Cuts at: 5242 7351 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1265 877 630 342 226 152 141 116 AseI AT'TA_AT Cuts at: 160 1032 6109 160 Size: 872 5077 1606 Fragments arranged by size: 5077 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7555 AvaI C'yCGr_G Cuts at: 1870 1943 2134 4880 1870 Size: 73 191 2746 4545 Fragments arranged by size: 4545 2746 191 73 BamHI G'GATC_C Cuts at: 4885 5252 4885 Size: 367 7188 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4876 6331 618 Size: 325 217 627 3089 1455 1842 Fragments arranged by size: 3089 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2060 2070 4259 4874 1391 Size: 669 10 2189 615 4072 Fragments arranged by size: 4072 2189 669 615 10 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2474 3564 4626 928 Size: 971 575 1090 1062 3857 Fragments arranged by size: 3857 1090 1062 971 575 BbvCI CC'TCA_GC Cuts at: 2149 3723 4509 2149 Size: 1574 786 5195 Fragments arranged by size: 5195 1574 786 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5746 5780 1903 Size: 34 3809 34 3678 Fragments arranged by size: 3809 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2449 2924 5447 6657 2449 Size: 475 2523 1210 3347 Fragments arranged by size: 3347 2523 1210 475 BclI T'GATC_A Cuts at: 1548 3699 4696 1548 Size: 2151 997 4407 Fragments arranged by size: 4407 2151 997 BfrBI ATG'CAT Cuts at: 2813 2813 Size: 7555 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2033 2185 6166 Size: 107 122 71 631 605 361 152 3981 Cuts at: 6166 136 Size: 1525 Fragments arranged by size: 3981 1525 631 605 361 152 122 107 71 BglII A'GATC_T Cuts at: 7550 7550 Size: 7555 BlpI GC'TnA_GC Cuts at: 3390 4972 3390 Size: 1582 5973 Bme1580I G_kGCm'C Cuts at: 1790 5616 6772 1790 Size: 3826 1156 2573 Fragments arranged by size: 3826 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7555 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3105 3378 4738 Size: 416 53 83 399 24 1688 273 1360 Cuts at: 4738 5286 6244 442 Size: 548 958 1753 Fragments arranged by size: 1753 1688 1360 958 548 416 399 273 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3962 1056 Size: 2906 4649 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2094 2629 3456 3864 3954 6215 1239 Size: 855 535 827 408 90 2261 2579 Fragments arranged by size: 2579 2261 855 827 535 408 90 Bpu10I CC'TnA_GC Cuts at: 2149 3723 4509 2149 Size: 1574 786 5195 Fragments arranged by size: 5195 1574 786 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2195 2339 2543 3736 4749 5680 6545 6843 2195 Size: 144 204 1193 1013 931 865 298 2907 Fragments arranged by size: 2907 1193 1013 931 865 298 204 144 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2070 4599 6218 882 Size: 617 571 2529 1619 2219 Fragments arranged by size: 2529 2219 1619 617 571 BsaAI yAC'GTr Cuts at: 493 1204 4016 4852 5346 493 Size: 711 2812 836 494 2702 Fragments arranged by size: 2812 2702 836 711 494 BsaBI GATnn'nnATC Cuts at: 7549 7549 Size: 7555 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5362 5744 6379 275 Size: 53 83 186 936 3829 382 635 1451 Fragments arranged by size: 3829 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2618 3441 3769 4222 5988 6386 6660 6807 7218 Size: 823 328 453 1766 398 274 147 411 Cuts at: 7218 2618 Size: 2955 Fragments arranged by size: 2955 1766 823 453 411 398 328 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2466 2496 2889 2919 1178 Size: 30 165 30 1063 30 393 30 5814 Fragments arranged by size: 5814 1063 393 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2928 1186 Size: 1742 5813 BseYI C'CCAG_C Cuts at: 6758 7254 6758 Size: 496 7059 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2219 3432 4572 1163 Size: 573 483 1213 1140 4146 Fragments arranged by size: 4146 1213 1140 573 483 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5005 5766 6794 1540 Size: 275 186 3004 761 1028 2301 Fragments arranged by size: 3004 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2060 2070 3349 3391 5616 5701 6772 2060 Size: 10 1279 42 2225 85 1071 2843 Fragments arranged by size: 2843 2225 1279 1071 85 42 10 BsmI GAATG_Cn' Cuts at: 2772 3367 5066 5159 2772 Size: 595 1699 93 5168 Fragments arranged by size: 5168 1699 595 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2060 2070 3349 3391 4259 4874 5616 Size: 399 270 10 1279 42 868 615 742 Cuts at: 5616 5701 6772 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1279 1071 868 742 615 399 270 85 42 10 BspEI T'CCGG_A Cuts at: 3769 7218 3769 Size: 3449 4106 BspHI T'CATG_A Cuts at: 4786 5444 7174 4786 Size: 658 1730 5167 Fragments arranged by size: 5167 1730 658 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2779 4910 844 Size: 1935 2131 3489 Fragments arranged by size: 3489 2131 1935 BsrBI CCG'CTC Cuts at: 1394 1823 2688 5442 1394 Size: 429 865 2754 3507 Fragments arranged by size: 3507 2754 865 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2428 6050 6224 65 Size: 1183 1180 3622 174 1396 Fragments arranged by size: 3622 1396 1183 1180 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6199 6386 1700 Size: 110 4389 187 2869 Fragments arranged by size: 4389 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7555 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7555 BssSI C'ACGA_G Cuts at: 5615 6627 5615 Size: 1012 6543 BstAPI GCAn_nnn'nTGC Cuts at: 794 3515 794 Size: 2721 4834 BstBI TT'CG_AA Cuts at: 2007 6359 2007 Size: 4352 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7555 BstXI CCAn_nnnn'nTGG Cuts at: 2924 3929 2924 Size: 1005 6550 BstYI r'GATC_y Cuts at: 1972 2672 4166 4885 5252 5637 5654 7106 7192 Size: 700 1494 719 367 385 17 1452 86 Cuts at: 7192 7204 7550 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1494 1452 719 700 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3558 3643 3558 Size: 85 7470 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 6046 Fragments arranged by size: 6046 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6816 Fragments arranged by size: 6816 683 56 BtsI GCAGTG_nn' Cuts at: 5071 5865 5885 5071 Size: 794 20 6741 Fragments arranged by size: 6741 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7555 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7520 DraI TTT'AAA Cuts at: 3129 4825 4859 3129 Size: 1696 34 5825 Fragments arranged by size: 5825 1696 34 DraIII CAC_nnn'GTG Cuts at: 3074 3074 Size: 7555 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6562 809 Size: 507 5246 1802 Fragments arranged by size: 5246 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5002 5891 7520 Size: 54 1425 50 275 18 3172 889 1629 Cuts at: 7520 8 Size: 43 Fragments arranged by size: 3172 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5002 1537 Size: 275 3190 4090 Fragments arranged by size: 4090 3190 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2089 2678 2895 2985 3216 3921 4320 4470 Size: 736 589 217 90 231 705 399 150 Cuts at: 4470 4572 5485 1353 Size: 102 913 3423 Fragments arranged by size: 3423 913 736 705 589 399 231 217 150 102 90 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6142 6516 6662 387 Size: 1064 138 4553 374 146 1280 Fragments arranged by size: 4553 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2058 2068 2058 Size: 10 7545 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2094 2629 3045 3219 3335 3456 3780 Size: 393 462 535 416 174 116 121 324 Cuts at: 3780 3798 3864 3954 5618 6215 7002 7270 1239 Size: 18 66 90 1664 597 787 268 1524 Fragments arranged by size: 1664 1524 787 597 535 462 416 393 324 268 174 121 116 90 66 18 EcoNI CCTnn'n_nnAGG Cuts at: 4475 4475 Size: 7555 EcoO109I rG'GnC_Cy Cuts at: 4281 4351 4281 Size: 70 7485 EcoRI G'AATT_C Cuts at: 4864 5242 4864 Size: 378 7177 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3227 3227 Size: 7555 FspI TGC'GCA Cuts at: 3853 6061 3853 Size: 2208 5347 HaeII r_GCGC'y Cuts at: 1678 2404 3378 6702 1678 Size: 726 974 3324 2531 Fragments arranged by size: 3324 2531 974 726 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2466 2498 2824 2856 3589 Size: 32 135 32 894 32 326 32 733 Cuts at: 3589 3621 4387 4419 6196 6228 6270 6302 1373 Size: 32 766 32 1777 32 42 32 2626 Fragments arranged by size: 2626 1777 894 766 733 326 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4842 4899 5145 669 Size: 988 281 2904 57 246 3079 Fragments arranged by size: 3079 2904 988 281 246 57 HindIII A'AGCT_T Cuts at: 748 2561 2885 748 Size: 1813 324 5418 Fragments arranged by size: 5418 1813 324 HpaI GTT'AAC Cuts at: 4842 5145 4842 Size: 303 7252 KpnI G_GTAC'C Cuts at: 4880 4880 Size: 7555 MfeI C'AATT_G Cuts at: 5154 5154 Size: 7555 MluI A'CGCG_T Cuts at: 7431 7431 Size: 7555 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2509 2560 2889 3760 4115 4585 6669 6853 Size: 1204 51 329 871 355 470 2084 184 Cuts at: 6853 1305 Size: 2007 Fragments arranged by size: 2084 2007 1204 871 470 355 329 184 51 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5733 Fragments arranged by size: 5733 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 5513 5872 6031 518 Size: 648 4347 359 159 2042 Fragments arranged by size: 4347 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2491 3925 4971 5648 6796 7041 1606 Size: 885 1434 1046 677 1148 245 2120 Fragments arranged by size: 2120 1434 1148 1046 885 677 245 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 6046 Fragments arranged by size: 6046 957 552 NdeI CA'TA_TG Cuts at: 387 2815 3704 387 Size: 2428 889 4238 Fragments arranged by size: 4238 2428 889 NheI G'CTAG_C Cuts at: 1052 3958 1052 Size: 2906 4649 NotI GC'GGCC_GC Cuts at: 5002 5002 Size: 7555 NsiI A_TGCA'T Cuts at: 2815 2815 Size: 7555 NspI r_CATG'y Cuts at: 1132 1229 3082 4098 4913 6458 1132 Size: 97 1853 1016 815 1545 2229 Fragments arranged by size: 2229 1853 1545 1016 815 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7555 PciI A'CATG_T Cuts at: 6454 6454 Size: 7555 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7555 PfoI T'CCnGG_A Cuts at: 2776 3874 2776 Size: 1098 6457 PmeI GTTT'AAAC Cuts at: 4859 4859 Size: 7555 PmlI CAC'GTG Cuts at: 4016 4016 Size: 7555 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2337 2369 5624 5656 7137 7169 2337 Size: 32 3255 32 1481 32 2723 Fragments arranged by size: 3255 2723 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4281 4351 4281 Size: 70 7485 PshAI GACnn'nnGTC Cuts at: 1613 2474 1613 Size: 861 6694 PsiI TTA'TAA Cuts at: 5125 5125 Size: 7555 PstI C_TGCA'G Cuts at: 830 3511 4907 830 Size: 2681 1396 3478 Fragments arranged by size: 3478 2681 1396 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7555 PvuII CAG'CTG Cuts at: 3925 3925 Size: 7555 SacI G_AGCT'C Cuts at: 2060 2070 2060 Size: 10 7545 SalI G'TCGA_C Cuts at: 1936 4897 1936 Size: 2961 4594 SapI GCTCTTCn'nnn_ Cuts at: 2678 2985 2678 Size: 307 7248 SbfI CC_TGCA'GG Cuts at: 4907 4907 Size: 7555 ScaI AGT'ACT Cuts at: 3417 5803 7515 3417 Size: 2386 1712 3457 Fragments arranged by size: 3457 2386 1712 SfcI C'TryA_G Cuts at: 826 1045 2963 3507 4153 4903 6038 6719 6910 Size: 219 1918 544 646 750 1135 681 191 Cuts at: 6910 826 Size: 1471 Fragments arranged by size: 1918 1471 1135 750 681 646 544 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7555 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7555 SmaI CCC'GGG Cuts at: 1872 4882 1872 Size: 3010 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2174 2354 2558 3751 4289 4764 Size: 197 926 231 180 204 1193 538 475 Cuts at: 4764 5659 6560 6822 820 Size: 895 901 262 1553 Fragments arranged by size: 1553 1193 926 901 895 538 475 262 231 204 197 180 SnaBI TAC'GTA Cuts at: 493 4852 5346 493 Size: 4359 494 2702 Fragments arranged by size: 4359 2702 494 SpeI A'CTAG_T Cuts at: 152 3110 152 Size: 2958 4597 SphI G_CATG'C Cuts at: 1132 4913 1132 Size: 3781 3774 SspI AAT'ATT Cuts at: 5 52 5479 5 Size: 47 5427 2081 Fragments arranged by size: 5427 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7555 StyI C'CwwG_G Cuts at: 513 1065 2022 3615 4994 513 Size: 552 957 1593 1379 3074 Fragments arranged by size: 3074 1593 1379 957 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5582 5599 5752 5937 6382 325 Size: 1255 4002 17 153 185 445 1498 Fragments arranged by size: 4002 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3415 3829 5801 Size: 274 80 33 51 1443 1438 414 1972 Cuts at: 5801 7513 96 Size: 1712 138 Fragments arranged by size: 1972 1712 1443 1438 414 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7515 7547 7515 Size: 32 7523 Tth111I GACn'n_nGTC Cuts at: 1316 2860 4793 1316 Size: 1544 1933 4078 Fragments arranged by size: 4078 1933 1544 XbaI T'CTAG_A Cuts at: 4891 4891 Size: 7555 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2029 2029 Size: 7555 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7555 XmaI C'CCGG_G Cuts at: 1870 4880 1870 Size: 3010 4545 XmnI GAAnn'nnTTC Cuts at: 3204 3717 3765 5684 3204 Size: 513 48 1919 5075 Fragments arranged by size: 5075 1919 513 48 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5363 276 Size: 53 83 186 936 3829 2468 Fragments arranged by size: 3829 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI