(Circular) (Six-base) MAPSORT of: pFN21ASDA1277.seq Check: 4002 from: 1 to: 7756 pFN21ASDA1277 7756 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4781 5566 278 Size: 53 83 186 936 3245 785 2468 Fragments arranged by size: 3245 2468 936 785 186 83 53 AccI GT'mk_AC Cuts at: 1937 4443 5099 1937 Size: 2506 656 4594 Fragments arranged by size: 4594 2506 656 Acc65I G'GTAC_C Cuts at: 3666 5077 3666 Size: 1411 6345 AclI AA'CG_TT Cuts at: 1563 2837 5883 6256 1563 Size: 1274 3046 373 3063 Fragments arranged by size: 3063 3046 1274 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2522 2872 3671 3818 3866 4027 4029 4589 Size: 890 350 799 147 48 161 2 560 Cuts at: 4589 4743 5030 5819 7203 7471 1632 Size: 154 287 789 1384 268 1917 Fragments arranged by size: 1917 1384 890 799 789 560 350 287 268 161 154 147 48 2 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7559 AflIII A'CryG_T Cuts at: 1203 2769 6655 7632 1203 Size: 1566 3886 977 1327 Fragments arranged by size: 3886 1566 1327 977 AgeI A'CCGG_T Cuts at: 6587 6587 Size: 7756 AhdI GACnn_n'nnGTC Cuts at: 4252 6485 4252 Size: 2233 5523 AleI CACnn'nnGTG Cuts at: 1166 4992 1166 Size: 3826 3930 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7756 AlwNI CAG_nnn'CTG Cuts at: 3628 4196 5499 7071 3628 Size: 568 1303 1572 4313 Fragments arranged by size: 4313 1572 1303 568 ApaI G_GGCC'C Cuts at: 4671 4671 Size: 7756 ApaLI G'TGCA_C Cuts at: 5813 6969 5813 Size: 1156 6600 ApoI r'AATT_y Cuts at: 1493 3220 3301 5033 5065 5291 5443 7552 1493 Size: 1727 81 1732 32 226 152 2109 1697 Fragments arranged by size: 2109 1732 1727 1697 226 152 81 32 AseI AT'TA_AT Cuts at: 160 1032 6310 160 Size: 872 5278 1606 Fragments arranged by size: 5278 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7756 AvaI C'yCGr_G Cuts at: 1870 1943 2519 3681 4235 5081 1870 Size: 73 576 1162 554 846 4545 Fragments arranged by size: 4545 1162 846 576 554 73 BamHI G'GATC_C Cuts at: 2428 2554 5086 5453 2428 Size: 126 2532 367 4731 Fragments arranged by size: 4731 2532 367 126 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2197 3658 3666 5077 6532 Size: 325 217 627 410 1461 8 1411 1455 Cuts at: 6532 618 Size: 1842 Fragments arranged by size: 1842 1461 1455 1411 627 410 325 217 8 BanII G_rGCy'C Cuts at: 1391 2703 2783 4671 4954 5075 1391 Size: 1312 80 1888 283 121 4072 Fragments arranged by size: 4072 1888 1312 283 121 80 BbeI G_GCGC'C Cuts at: 3662 3662 Size: 7756 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2983 3042 3141 3979 4858 928 Size: 971 1084 59 99 838 879 3826 Fragments arranged by size: 3826 1084 971 879 838 99 59 BbvCI CC'TCA_GC Cuts at: 3834 4271 3834 Size: 437 7319 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3644 3678 3940 3974 5947 5981 1903 Size: 34 1707 34 262 34 1973 34 3678 Fragments arranged by size: 3678 1973 1707 262 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5648 6858 5648 Size: 1210 6546 BclI T'GATC_A Cuts at: 1548 2046 1548 Size: 498 7258 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2078 6367 136 Size: 107 122 71 631 605 406 4289 1525 Fragments arranged by size: 4289 1525 631 605 406 122 107 71 BglII A'GATC_T Cuts at: 7751 7751 Size: 7756 BlpI GC'TnA_GC Cuts at: 4346 5173 4346 Size: 827 6929 Bme1580I G_kGCm'C Cuts at: 1790 2202 4671 5817 6973 1790 Size: 412 2469 1146 1156 2573 Fragments arranged by size: 2573 2469 1156 1146 412 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7756 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2554 3369 3529 Size: 416 53 83 399 24 1137 815 160 Cuts at: 3529 3853 4971 5487 6445 442 Size: 324 1118 516 958 1753 Fragments arranged by size: 1753 1137 1118 958 815 516 416 399 324 160 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7756 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3568 3568 Size: 7756 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3957 4005 4334 4471 4880 6416 1239 Size: 2718 48 329 137 409 1536 2579 Fragments arranged by size: 2718 2579 1536 409 329 137 48 Bpu10I CC'TnA_GC Cuts at: 2405 2709 3834 4271 4505 2405 Size: 304 1125 437 234 5656 Fragments arranged by size: 5656 1125 437 304 234 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2783 5881 6746 7044 2783 Size: 3098 865 298 3495 Fragments arranged by size: 3495 3098 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3423 6419 882 Size: 617 1924 2996 2219 Fragments arranged by size: 2996 2219 1924 617 BsaAI yAC'GTr Cuts at: 493 1204 3769 4243 5053 5547 493 Size: 711 2565 474 810 494 2702 Fragments arranged by size: 2702 2565 810 711 494 474 BsaBI GATnn'nnATC Cuts at: 7750 7750 Size: 7756 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3659 4463 4778 5563 Size: 53 83 186 936 2126 804 315 785 Cuts at: 5563 5945 6580 275 Size: 382 635 1451 Fragments arranged by size: 2126 1451 936 804 785 635 382 315 186 83 53 BsaWI w'CCGG_w Cuts at: 2754 3501 6189 6587 6861 7008 7419 2754 Size: 747 2688 398 274 147 411 3091 Fragments arranged by size: 3091 2688 747 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2260 2290 1178 Size: 30 165 30 857 30 6644 Fragments arranged by size: 6644 857 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2281 2284 2904 2938 3134 3325 3614 4255 Size: 1095 3 620 34 196 191 289 641 Cuts at: 4255 4333 4808 4825 1186 Size: 78 475 17 4117 Fragments arranged by size: 4117 1095 641 620 475 289 196 191 78 34 17 3 BseYI C'CCAG_C Cuts at: 2059 2081 2213 2436 3094 3395 4144 6959 7455 Size: 22 132 223 658 301 749 2815 496 Cuts at: 7455 2059 Size: 2360 Fragments arranged by size: 2815 2360 749 658 496 301 223 132 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2193 3027 1163 Size: 573 457 834 5892 Fragments arranged by size: 5892 834 573 457 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5206 5967 6995 1540 Size: 275 186 3205 761 1028 2301 Fragments arranged by size: 3205 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2339 4242 4457 4989 5817 5902 6973 2339 Size: 1903 215 532 828 85 1071 3122 Fragments arranged by size: 3122 1903 1071 828 532 215 85 BsmI GAATG_Cn' Cuts at: 5267 5360 5267 Size: 93 7663 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2202 2339 2703 2783 4242 4457 4671 Size: 399 412 137 364 80 1459 215 214 Cuts at: 4671 4954 4989 5075 5817 5902 6973 1391 Size: 283 35 86 742 85 1071 2174 Fragments arranged by size: 2174 1459 1071 742 412 399 364 283 215 214 137 86 85 80 35 BspEI T'CCGG_A Cuts at: 3501 7419 3501 Size: 3918 3838 BspHI T'CATG_A Cuts at: 3822 4116 5645 7375 3822 Size: 294 1529 1730 4203 Fragments arranged by size: 4203 1730 1529 294 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2497 3292 5111 844 Size: 1653 795 1819 3489 Fragments arranged by size: 3489 1819 1653 795 BsrBI CCG'CTC Cuts at: 1394 1823 4906 5643 1394 Size: 429 3083 737 3507 Fragments arranged by size: 3507 3083 737 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4332 6251 6425 65 Size: 1183 3084 1919 174 1396 Fragments arranged by size: 3084 1919 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4554 4917 6400 6587 1700 Size: 110 2744 363 1483 187 2869 Fragments arranged by size: 2869 2744 1483 363 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7756 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7756 BssSI C'ACGA_G Cuts at: 5816 6828 5816 Size: 1012 6744 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7756 BstBI TT'CG_AA Cuts at: 2007 6560 2007 Size: 4553 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7756 BstYI r'GATC_y Cuts at: 1972 2428 2554 3145 4059 5086 5453 5838 5855 Size: 456 126 591 914 1027 367 385 17 Cuts at: 5855 7307 7393 7405 7751 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1027 914 591 456 385 367 346 126 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 4437 513 Size: 552 957 2415 3832 Fragments arranged by size: 3832 2415 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4388 487 Size: 683 56 3162 3855 Fragments arranged by size: 3855 3162 683 56 BtsI GCAGTG_nn' Cuts at: 2370 2587 4499 5272 6066 6086 2370 Size: 217 1912 773 794 20 4040 Fragments arranged by size: 4040 1912 794 773 217 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7756 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7721 DraI TTT'AAA Cuts at: 5060 5060 Size: 7756 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6763 809 Size: 507 5447 1802 Fragments arranged by size: 5447 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5203 6092 7721 Size: 54 1425 50 275 18 3373 889 1629 Cuts at: 7721 8 Size: 43 Fragments arranged by size: 3373 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5203 1537 Size: 275 3391 4090 Fragments arranged by size: 4090 3391 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3247 3446 3518 3719 4265 4518 5686 1353 Size: 1894 199 72 201 546 253 1168 3423 Fragments arranged by size: 3423 1894 1168 546 253 201 199 72 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3138 4268 4838 6343 6717 6863 Size: 1064 138 1549 1130 570 1505 374 146 Cuts at: 6863 387 Size: 1280 Fragments arranged by size: 1549 1505 1280 1130 1064 570 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2522 2872 3671 3818 3866 3957 4005 Size: 393 890 350 799 147 48 91 48 Cuts at: 4005 4027 4029 4334 4471 4589 4743 4880 5030 Size: 22 2 305 137 118 154 137 150 Cuts at: 5030 5819 6416 7203 7471 1239 Size: 789 597 787 268 1524 Fragments arranged by size: 1524 890 799 789 787 597 393 350 305 268 154 150 147 137 137 118 91 48 48 22 2 EcoO109I rG'GnC_Cy Cuts at: 2383 2745 3363 4528 4667 4668 2383 Size: 362 618 1165 139 1 5471 Fragments arranged by size: 5471 1165 618 362 139 1 EcoRI G'AATT_C Cuts at: 5065 5443 5065 Size: 378 7378 EcoRV GAT'ATC Cuts at: 2172 2172 Size: 7756 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2491 3232 4547 2491 Size: 741 1315 5700 Fragments arranged by size: 5700 1315 741 FspI TGC'GCA Cuts at: 6262 6262 Size: 7756 HaeII r_GCGC'y Cuts at: 1678 3628 3662 4003 4385 6903 1678 Size: 1950 34 341 382 2518 2531 Fragments arranged by size: 2531 2518 1950 382 341 34 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2156 2188 2477 2509 2600 Size: 32 135 32 584 32 289 32 91 Cuts at: 2600 2632 4074 4106 4238 4270 4568 4600 4703 Size: 32 1442 32 132 32 298 32 103 Cuts at: 4703 4735 6397 6429 6471 6503 1373 Size: 32 1662 32 42 32 2626 Fragments arranged by size: 2626 1662 1442 584 298 289 135 132 103 91 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3634 4444 4540 4549 5100 5346 Size: 988 281 1696 810 96 9 551 246 Cuts at: 5346 669 Size: 3079 Fragments arranged by size: 3079 1696 988 810 551 281 246 96 9 HindIII A'AGCT_T Cuts at: 748 4601 748 Size: 3853 3903 HpaI GTT'AAC Cuts at: 5346 5346 Size: 7756 KasI G'GCGC_C Cuts at: 3658 3658 Size: 7756 KpnI G_GTAC'C Cuts at: 3670 5081 3670 Size: 1411 6345 MfeI C'AATT_G Cuts at: 5355 5355 Size: 7756 MluI A'CGCG_T Cuts at: 7632 7632 Size: 7756 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3319 3783 6870 7054 1305 Size: 2014 464 3087 184 2007 Fragments arranged by size: 3087 2014 2007 464 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5934 Fragments arranged by size: 5934 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2027 4421 4992 5714 6073 6232 518 Size: 648 861 2394 571 722 359 159 2042 Fragments arranged by size: 2394 2042 861 722 648 571 359 159 MspA1I CmG'CkG Cuts at: 1606 2063 4222 4519 4810 4992 5172 5849 6997 Size: 457 2159 297 291 182 180 677 1148 Cuts at: 6997 7242 1606 Size: 245 2120 Fragments arranged by size: 2159 2120 1148 677 457 297 291 245 182 180 NaeI GCC'GGC Cuts at: 4919 4919 Size: 7756 NarI GG'CG_CC Cuts at: 3659 3659 Size: 7756 NcoI C'CATG_G Cuts at: 513 1065 2022 4437 513 Size: 552 957 2415 3832 Fragments arranged by size: 3832 2415 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7756 NgoMIV G'CCGG_C Cuts at: 4917 4917 Size: 7756 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7756 NotI GC'GGCC_GC Cuts at: 5203 5203 Size: 7756 NspI r_CATG'y Cuts at: 1132 1229 2849 5114 6659 1132 Size: 97 1620 2265 1545 2229 Fragments arranged by size: 2265 2229 1620 1545 97 PasI CC'CwG_GG Cuts at: 1348 2748 4150 1348 Size: 1400 1402 4954 Fragments arranged by size: 4954 1402 1400 PciI A'CATG_T Cuts at: 6655 6655 Size: 7756 PflMI CCAn_nnn'nTGG Cuts at: 1108 2462 3541 3628 3885 4375 4858 1108 Size: 1354 1079 87 257 490 483 4006 Fragments arranged by size: 4006 1354 1079 490 483 257 87 PfoI T'CCnGG_A Cuts at: 4614 4857 4899 4614 Size: 243 42 7471 Fragments arranged by size: 7471 243 42 PmeI GTTT'AAAC Cuts at: 5060 5060 Size: 7756 PmlI CAC'GTG Cuts at: 4243 4243 Size: 7756 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5825 5857 7338 7370 5825 Size: 32 1481 32 6211 Fragments arranged by size: 6211 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2383 3363 4528 2383 Size: 980 1165 5611 Fragments arranged by size: 5611 1165 980 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7756 PsiI TTA'TAA Cuts at: 5326 5326 Size: 7756 PspOMI G'GGCC_C Cuts at: 4667 4667 Size: 7756 PspXI vC'TCGA_Gb Cuts at: 2519 4235 2519 Size: 1716 6040 PstI C_TGCA'G Cuts at: 830 2068 3048 4809 5108 830 Size: 1238 980 1761 299 3478 Fragments arranged by size: 3478 1761 1238 980 299 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7756 PvuII CAG'CTG Cuts at: 2063 2063 Size: 7756 SalI G'TCGA_C Cuts at: 1936 4442 5098 1936 Size: 2506 656 4594 Fragments arranged by size: 4594 2506 656 SanDI GG'GwC_CC Cuts at: 3363 3363 Size: 7756 SapI GCTCTTCn'nnn_ Cuts at: 3719 3719 Size: 7756 SbfI CC_TGCA'GG Cuts at: 5108 5108 Size: 7756 ScaI AGT'ACT Cuts at: 3722 6004 7716 3722 Size: 2282 1712 3762 Fragments arranged by size: 3762 2282 1712 SexAI A'CCwGG_T Cuts at: 4284 4284 Size: 7756 SfcI C'TryA_G Cuts at: 826 1045 2064 3044 4805 5104 6239 6920 7111 Size: 219 1019 980 1761 299 1135 681 191 Cuts at: 7111 826 Size: 1471 Fragments arranged by size: 1761 1471 1135 1019 980 681 299 219 191 SfoI GGC'GCC Cuts at: 3660 3660 Size: 7756 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7756 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7756 SmaI CCC'GGG Cuts at: 1872 3683 5083 1872 Size: 1811 1400 4545 Fragments arranged by size: 4545 1811 1400 SmlI C'TyrA_G Cuts at: 820 1017 1943 2519 2762 4235 5860 6761 7023 Size: 197 926 576 243 1473 1625 901 262 Cuts at: 7023 820 Size: 1553 Fragments arranged by size: 1625 1553 1473 926 901 576 262 243 197 SnaBI TAC'GTA Cuts at: 493 5053 5547 493 Size: 4560 494 2702 Fragments arranged by size: 4560 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7756 SphI G_CATG'C Cuts at: 1132 5114 1132 Size: 3982 3774 SspI AAT'ATT Cuts at: 5 52 5680 5 Size: 47 5628 2081 Fragments arranged by size: 5628 2081 47 StuI AGG'CCT Cuts at: 1517 4817 1517 Size: 3300 4456 StyI C'CwwG_G Cuts at: 513 1065 2022 3424 3879 4437 5195 513 Size: 552 957 1402 455 558 758 3074 Fragments arranged by size: 3074 1402 957 758 558 552 455 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2227 3797 5783 5800 5953 6138 6583 Size: 1255 647 1570 1986 17 153 185 445 Cuts at: 6583 325 Size: 1498 Fragments arranged by size: 1986 1570 1498 1255 647 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3720 6002 7714 Size: 274 80 33 51 1443 1743 2282 1712 Cuts at: 7714 96 Size: 138 Fragments arranged by size: 2282 1743 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2260 2292 3005 3037 4185 4217 7716 7748 2260 Size: 32 713 32 1148 32 3499 32 2268 Fragments arranged by size: 3499 2268 1148 713 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4544 1316 Size: 3228 4528 XbaI T'CTAG_A Cuts at: 3142 5092 3142 Size: 1950 5806 XhoI C'TCGA_G Cuts at: 1943 2519 4235 1943 Size: 576 1716 5464 Fragments arranged by size: 5464 1716 576 XmaI C'CCGG_G Cuts at: 1870 3681 5081 1870 Size: 1811 1400 4545 Fragments arranged by size: 4545 1811 1400 XmnI GAAnn'nnTTC Cuts at: 3138 4623 5885 3138 Size: 1485 1262 5009 Fragments arranged by size: 5009 1485 1262 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4779 5564 276 Size: 53 83 186 936 3245 785 2468 Fragments arranged by size: 3245 2468 936 785 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI NruI NsiI PacI PsrI RsrII SacI SacII SfiI SrfI SwaI XcmI