(Circular) (Six-base) MAPSORT of: pFN21ASDA1276.seq Check: 955 from: 1 to: 7909 pFN21ASDA1276 7909 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5719 278 Size: 53 83 186 936 4183 2468 Fragments arranged by size: 4183 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5087 5252 1937 Size: 3150 165 4594 Fragments arranged by size: 4594 3150 165 Acc65I G'GTAC_C Cuts at: 5230 5230 Size: 7909 AclI AA'CG_TT Cuts at: 1563 6036 6409 1563 Size: 4473 373 3063 Fragments arranged by size: 4473 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2900 2921 3705 4727 4793 5972 7356 7624 Size: 1268 21 784 1022 66 1179 1384 268 Cuts at: 7624 1632 Size: 1917 Fragments arranged by size: 1917 1384 1268 1179 1022 784 268 66 21 AflII C'TTAA_G Cuts at: 820 1017 2933 820 Size: 197 1916 5796 Fragments arranged by size: 5796 1916 197 AflIII A'CryG_T Cuts at: 1203 6808 7785 1203 Size: 5605 977 1327 Fragments arranged by size: 5605 1327 977 AgeI A'CCGG_T Cuts at: 6740 6740 Size: 7909 AhdI GACnn_n'nnGTC Cuts at: 6638 6638 Size: 7909 AleI CACnn'nnGTG Cuts at: 1166 2162 1166 Size: 996 6913 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3069 3203 4263 1184 Size: 1885 134 1060 4830 Fragments arranged by size: 4830 1885 1060 134 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4949 4981 4949 Size: 32 7877 AlwNI CAG_nnn'CTG Cuts at: 2332 2515 2629 2650 2752 2899 2920 3563 3868 Size: 183 114 21 102 147 21 643 305 Cuts at: 3868 4204 4231 5652 7224 2332 Size: 336 27 1421 1572 3017 Fragments arranged by size: 3017 1572 1421 643 336 305 183 147 114 102 27 21 21 ApaI G_GGCC'C Cuts at: 2594 2594 Size: 7909 ApaLI G'TGCA_C Cuts at: 3877 5966 7122 3877 Size: 2089 1156 4664 Fragments arranged by size: 4664 2089 1156 ApoI r'AATT_y Cuts at: 1493 2953 3496 3527 5218 5444 5596 7705 1493 Size: 1460 543 31 1691 226 152 2109 1697 Fragments arranged by size: 2109 1697 1691 1460 543 226 152 31 AseI AT'TA_AT Cuts at: 160 1032 5010 6463 160 Size: 872 3978 1453 1606 Fragments arranged by size: 3978 1606 1453 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7909 AvaI C'yCGr_G Cuts at: 1870 1943 3998 4381 4733 4885 5234 1870 Size: 73 2055 383 352 152 349 4545 Fragments arranged by size: 4545 2055 383 352 349 152 73 BamHI G'GATC_C Cuts at: 3619 5239 5606 3619 Size: 1620 367 5922 Fragments arranged by size: 5922 1620 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2090 4260 5230 6685 618 Size: 325 217 627 303 2170 970 1455 1842 Fragments arranged by size: 2170 1842 1455 970 627 325 303 217 BanII G_rGCy'C Cuts at: 1391 2075 2244 2484 2564 2594 3767 3957 4021 Size: 684 169 240 80 30 1173 190 64 Cuts at: 4021 4134 4303 4443 5165 5228 1391 Size: 113 169 140 722 63 4072 Fragments arranged by size: 4072 1173 722 684 240 190 169 169 140 113 80 64 63 30 BbeI G_GCGC'C Cuts at: 2094 2094 Size: 7909 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3068 3252 3327 928 Size: 971 1169 184 75 5510 Fragments arranged by size: 5510 1169 971 184 75 BbvCI CC'TCA_GC Cuts at: 2199 2423 3132 3564 4295 2199 Size: 224 709 432 731 5813 Fragments arranged by size: 5813 731 709 432 224 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2128 2162 3014 3048 6100 6134 1903 Size: 34 191 34 852 34 3052 34 3678 Fragments arranged by size: 3678 3052 852 191 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5801 7011 5801 Size: 1210 6699 BclI T'GATC_A Cuts at: 1548 1548 Size: 7909 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2672 2819 4392 Size: 107 122 71 631 605 1000 147 1573 Cuts at: 4392 4571 4628 4670 6520 136 Size: 179 57 42 1850 1525 Fragments arranged by size: 1850 1573 1525 1000 631 605 179 147 122 107 71 57 42 BglII A'GATC_T Cuts at: 7904 7904 Size: 7909 BlpI GC'TnA_GC Cuts at: 2651 5326 2651 Size: 2675 5234 Bme1580I G_kGCm'C Cuts at: 1790 2594 3164 3881 4263 4648 4935 5970 7126 Size: 804 570 717 382 385 287 1035 1156 Cuts at: 7126 1790 Size: 2573 Fragments arranged by size: 2573 1156 1035 804 717 570 385 382 287 BmgBI CAC'GTC Cuts at: 1318 4930 1318 Size: 3612 4297 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3770 4833 5640 Size: 416 53 83 399 24 2353 1063 807 Cuts at: 5640 6598 442 Size: 958 1753 Fragments arranged by size: 2353 1753 1063 958 807 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7909 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2407 2407 Size: 7909 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2119 2228 2354 2366 2423 2449 2492 2498 Size: 880 109 126 12 57 26 43 6 Cuts at: 2498 2870 3476 3629 3890 3941 3950 4052 4085 Size: 372 606 153 261 51 9 102 33 Cuts at: 4085 4118 4148 4448 4568 4643 6569 1239 Size: 33 30 300 120 75 1926 2579 Fragments arranged by size: 2579 1926 880 606 372 300 261 153 126 120 109 102 75 57 51 43 33 33 30 26 12 9 6 Bpu10I CC'TnA_GC Cuts at: 2199 2423 2823 3132 3564 4295 2199 Size: 224 400 309 432 731 5813 Fragments arranged by size: 5813 731 432 400 309 224 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2468 3137 3527 3716 3896 4040 6034 6899 7197 Size: 669 390 189 180 144 1994 865 298 Cuts at: 7197 2468 Size: 3180 Fragments arranged by size: 3180 1994 865 669 390 298 189 180 144 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2255 2838 3605 3853 3956 4524 6572 Size: 617 756 583 767 248 103 568 2048 Cuts at: 6572 882 Size: 2219 Fragments arranged by size: 2219 2048 767 756 617 583 568 248 103 BsaAI yAC'GTr Cuts at: 493 1204 2128 5206 5700 493 Size: 711 924 3078 494 2702 Fragments arranged by size: 3078 2702 924 711 494 BsaBI GATnn'nnATC Cuts at: 7903 7903 Size: 7909 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2091 5716 6098 6733 Size: 53 83 186 936 558 3625 382 635 Cuts at: 6733 275 Size: 1451 Fragments arranged by size: 3625 1451 936 635 558 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2829 4605 4700 4854 6342 6740 7014 7161 7572 Size: 1776 95 154 1488 398 274 147 411 Cuts at: 7572 2829 Size: 3166 Fragments arranged by size: 3166 1776 1488 411 398 274 154 147 95 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2759 2789 4116 4146 4949 Size: 30 165 30 1356 30 1327 30 803 Cuts at: 4949 4979 1178 Size: 30 4108 Fragments arranged by size: 4108 1356 1327 803 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2252 2315 2384 2515 2693 3293 3669 3674 Size: 1066 63 69 131 178 600 376 5 Cuts at: 3674 3821 4187 4382 4463 4565 1186 Size: 147 366 195 81 102 4530 Fragments arranged by size: 4530 1066 600 376 366 195 178 147 131 102 81 69 63 5 BseYI C'CCAG_C Cuts at: 2151 3993 4412 4566 4653 4934 5036 5054 5148 Size: 1842 419 154 87 281 102 18 94 Cuts at: 5148 7112 7608 2151 Size: 1964 496 2452 Fragments arranged by size: 2452 1964 1842 496 419 281 154 102 94 87 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2416 2642 2948 1163 Size: 573 680 226 306 6124 Fragments arranged by size: 6124 680 573 306 226 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5359 6120 7148 1540 Size: 275 186 3358 761 1028 2301 Fragments arranged by size: 3358 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2244 2442 2484 2856 3881 3957 4134 5970 6055 Size: 198 42 372 1025 76 177 1836 85 Cuts at: 6055 7126 2244 Size: 1071 3027 Fragments arranged by size: 3027 1836 1071 1025 372 198 177 85 76 42 BsmI GAATG_Cn' Cuts at: 2254 3603 4832 5420 5513 2254 Size: 1349 1229 588 93 4650 Fragments arranged by size: 4650 1349 1229 588 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2399 2399 Size: 7909 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2075 2244 2442 2484 2564 2594 2856 Size: 399 285 169 198 42 80 30 262 Cuts at: 2856 3164 3767 3881 3957 4021 4134 4263 4303 Size: 308 603 114 76 64 113 129 40 Cuts at: 4303 4443 4648 4935 5165 5228 5970 6055 7126 Size: 140 205 287 230 63 742 85 1071 Cuts at: 7126 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 603 399 308 287 285 262 230 205 198 169 140 129 114 113 85 80 76 64 63 42 40 30 BspEI T'CCGG_A Cuts at: 2829 4700 7572 2829 Size: 1871 2872 3166 Fragments arranged by size: 3166 2872 1871 BspHI T'CATG_A Cuts at: 5798 7528 5798 Size: 1730 6179 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3909 4530 5264 844 Size: 3065 621 734 3489 Fragments arranged by size: 3489 3065 734 621 BsrBI CCG'CTC Cuts at: 1394 1823 5796 1394 Size: 429 3973 3507 Fragments arranged by size: 3973 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6404 6578 65 Size: 1183 5156 174 1396 Fragments arranged by size: 5156 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2093 2544 3116 4391 6553 6740 1700 Size: 110 283 451 572 1275 2162 187 2869 Fragments arranged by size: 2869 2162 1275 572 451 283 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7909 BssHII G'CGCG_C Cuts at: 1925 4486 1925 Size: 2561 5348 BssSI C'ACGA_G Cuts at: 2392 2525 5969 6981 2392 Size: 133 3444 1012 3320 Fragments arranged by size: 3444 3320 1012 133 BstAPI GCAn_nnn'nTGC Cuts at: 794 2629 2728 4165 4204 5102 794 Size: 1835 99 1437 39 898 3601 Fragments arranged by size: 3601 1835 1437 898 99 39 BstBI TT'CG_AA Cuts at: 2007 6713 2007 Size: 4706 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7909 BstXI CCAn_nnnn'nTGG Cuts at: 4582 4942 4582 Size: 360 7549 BstYI r'GATC_y Cuts at: 1972 3619 4104 5239 5606 5991 6008 7460 7546 Size: 1647 485 1135 367 385 17 1452 86 Cuts at: 7546 7558 7904 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1647 1452 1135 485 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 3342 3369 4623 513 Size: 552 957 1320 27 1254 3799 Fragments arranged by size: 3799 1320 1254 957 552 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7170 Fragments arranged by size: 7170 683 56 BtsI GCAGTG_nn' Cuts at: 2765 2970 3595 3706 3881 4280 4589 5425 6219 Size: 205 625 111 175 399 309 836 794 Cuts at: 6219 6239 2765 Size: 20 4435 Fragments arranged by size: 4435 836 794 625 399 309 205 175 111 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7909 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7874 DraI TTT'AAA Cuts at: 5213 5213 Size: 7909 DraIII CAC_nnn'GTG Cuts at: 3343 3343 Size: 7909 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6916 809 Size: 507 5600 1802 Fragments arranged by size: 5600 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4753 5062 5356 Size: 54 1425 50 275 18 2923 309 294 Cuts at: 5356 6245 7874 8 Size: 889 1629 43 Fragments arranged by size: 2923 1629 1425 889 309 294 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5356 1537 Size: 275 3544 4090 Fragments arranged by size: 4090 3544 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2351 3093 3270 3459 4803 5839 1353 Size: 998 742 177 189 1344 1036 3423 Fragments arranged by size: 3423 1344 1036 998 742 189 177 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6496 6870 7016 387 Size: 1064 138 4907 374 146 1280 Fragments arranged by size: 4907 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2242 2482 3955 4132 2242 Size: 240 1473 177 6019 Fragments arranged by size: 6019 1473 240 177 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2119 2228 2354 2366 2423 2449 2492 Size: 393 487 109 126 12 57 26 43 Cuts at: 2492 2498 2870 2900 2921 3476 3629 3705 3890 Size: 6 372 30 21 555 153 76 185 Cuts at: 3890 3941 3950 4052 4085 4118 4148 4448 4568 Size: 51 9 102 33 33 30 300 120 Cuts at: 4568 4643 4727 4793 5972 6569 7356 7624 1239 Size: 75 84 66 1179 597 787 268 1524 Fragments arranged by size: 1524 1179 787 597 555 487 393 372 300 268 185 153 126 120 109 102 84 76 75 66 57 51 43 33 33 30 30 26 21 12 9 6 EcoNI CCTnn'n_nnAGG Cuts at: 2395 2615 2698 2395 Size: 220 83 7606 Fragments arranged by size: 7606 220 83 EcoO109I rG'GnC_Cy Cuts at: 2329 2590 2608 2749 3898 4201 4738 5112 2329 Size: 261 18 141 1149 303 537 374 5126 Fragments arranged by size: 5126 1149 537 374 303 261 141 18 EcoRI G'AATT_C Cuts at: 2953 3496 5218 5596 2953 Size: 543 1722 378 5266 Fragments arranged by size: 5266 1722 543 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3266 3266 Size: 7909 FspI TGC'GCA Cuts at: 2296 4025 6415 2296 Size: 1729 2390 3790 Fragments arranged by size: 3790 2390 1729 HaeII r_GCGC'y Cuts at: 1678 2094 2219 2343 2378 4410 7056 1678 Size: 416 125 124 35 2032 2646 2531 Fragments arranged by size: 2646 2531 2032 416 125 124 35 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2759 2791 3604 3636 3770 Size: 32 135 32 1187 32 813 32 134 Cuts at: 3770 3802 4116 4148 4425 4457 4773 4805 4978 Size: 32 314 32 277 32 316 32 173 Cuts at: 4978 5010 6550 6582 6624 6656 1373 Size: 32 1540 32 42 32 2626 Fragments arranged by size: 2626 1540 1187 813 316 314 277 173 135 134 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5253 5499 669 Size: 988 281 3315 246 3079 Fragments arranged by size: 3315 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7909 HpaI GTT'AAC Cuts at: 5499 5499 Size: 7909 KasI G'GCGC_C Cuts at: 2090 2090 Size: 7909 KpnI G_GTAC'C Cuts at: 5234 5234 Size: 7909 MfeI C'AATT_G Cuts at: 5508 5508 Size: 7909 MluI A'CGCG_T Cuts at: 7785 7785 Size: 7909 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2556 2757 3396 3559 4132 4215 7023 7207 Size: 1251 201 639 163 573 83 2808 184 Cuts at: 7207 1305 Size: 2007 Fragments arranged by size: 2808 2007 1251 639 573 201 184 163 83 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6087 Fragments arranged by size: 6087 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2162 2922 3185 5867 6226 6385 518 Size: 648 996 760 263 2682 359 159 2042 Fragments arranged by size: 2682 2042 996 760 648 359 263 159 MspA1I CmG'CkG Cuts at: 1606 2626 2650 3457 3683 4570 5058 5325 6002 Size: 1020 24 807 226 887 488 267 677 Cuts at: 6002 7150 7395 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 1020 887 807 677 488 267 245 226 24 NaeI GCC'GGC Cuts at: 2095 2546 3118 4393 2095 Size: 451 572 1275 5611 Fragments arranged by size: 5611 1275 572 451 NarI GG'CG_CC Cuts at: 2091 2091 Size: 7909 NcoI C'CATG_G Cuts at: 513 1065 2022 4623 513 Size: 552 957 2601 3799 Fragments arranged by size: 3799 2601 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7909 NgoMIV G'CCGG_C Cuts at: 2093 2544 3116 4391 2093 Size: 451 572 1275 5611 Fragments arranged by size: 5611 1275 572 451 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7909 NotI GC'GGCC_GC Cuts at: 5356 5356 Size: 7909 NspI r_CATG'y Cuts at: 1132 1229 4030 5267 6812 1132 Size: 97 2801 1237 1545 2229 Fragments arranged by size: 2801 2229 1545 1237 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7909 PciI A'CATG_T Cuts at: 6808 6808 Size: 7909 PflMI CCAn_nnn'nTGG Cuts at: 1108 2332 4141 1108 Size: 1224 1809 4876 Fragments arranged by size: 4876 1809 1224 PfoI T'CCnGG_A Cuts at: 2802 3957 4333 4464 4545 5003 2802 Size: 1155 376 131 81 458 5708 Fragments arranged by size: 5708 1155 458 376 131 81 PmeI GTTT'AAAC Cuts at: 5213 5213 Size: 7909 PmlI CAC'GTG Cuts at: 2128 2128 Size: 7909 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4949 4981 5978 6010 7491 7523 4949 Size: 32 997 32 1481 32 5335 Fragments arranged by size: 5335 1481 997 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2608 3898 4738 5112 2608 Size: 1290 840 374 5405 Fragments arranged by size: 5405 1290 840 374 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7909 PsiI TTA'TAA Cuts at: 5479 5479 Size: 7909 PspOMI G'GGCC_C Cuts at: 2590 2590 Size: 7909 PspXI vC'TCGA_Gb Cuts at: 3998 3998 Size: 7909 PstI C_TGCA'G Cuts at: 830 2283 2355 2688 2820 3027 3396 3602 3906 Size: 1453 72 333 132 207 369 206 304 Cuts at: 3906 3948 4170 4287 4764 5261 830 Size: 42 222 117 477 497 3478 Fragments arranged by size: 3478 1453 497 477 369 333 304 222 207 206 132 117 72 42 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7909 PvuII CAG'CTG Cuts at: 2650 3457 3683 5058 2650 Size: 807 226 1375 5501 Fragments arranged by size: 5501 1375 807 226 SacI G_AGCT'C Cuts at: 2244 2484 3957 4134 2244 Size: 240 1473 177 6019 Fragments arranged by size: 6019 1473 240 177 SalI G'TCGA_C Cuts at: 1936 5251 1936 Size: 3315 4594 SbfI CC_TGCA'GG Cuts at: 2283 2820 3906 5261 2283 Size: 537 1086 1355 4931 Fragments arranged by size: 4931 1355 1086 537 ScaI AGT'ACT Cuts at: 5192 6157 7869 5192 Size: 965 1712 5232 Fragments arranged by size: 5232 1712 965 SfcI C'TryA_G Cuts at: 826 1045 2279 2315 2351 2684 2816 3023 3392 Size: 219 1234 36 36 333 132 207 369 Cuts at: 3392 3598 3889 3902 3944 4166 4283 4760 5088 Size: 206 291 13 42 222 117 477 328 Cuts at: 5088 5257 6392 7073 7264 826 Size: 169 1135 681 191 1471 Fragments arranged by size: 1471 1234 1135 681 477 369 333 328 291 222 219 207 206 191 169 132 117 42 36 36 13 SfoI GGC'GCC Cuts at: 2092 2092 Size: 7909 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7909 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7909 SmaI CCC'GGG Cuts at: 1872 4383 4887 5236 1872 Size: 2511 504 349 4545 Fragments arranged by size: 4545 2511 504 349 SmlI C'TyrA_G Cuts at: 820 1017 1943 2483 2933 3152 3506 3731 3911 Size: 197 926 540 450 219 354 225 180 Cuts at: 3911 3998 4055 6013 6914 7176 820 Size: 87 57 1958 901 262 1553 Fragments arranged by size: 1958 1553 926 901 540 450 354 262 225 219 197 180 87 57 SnaBI TAC'GTA Cuts at: 493 5206 5700 493 Size: 4713 494 2702 Fragments arranged by size: 4713 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7909 SphI G_CATG'C Cuts at: 1132 4030 5267 1132 Size: 2898 1237 3774 Fragments arranged by size: 3774 2898 1237 SrfI GCCC'GGGC Cuts at: 4383 4383 Size: 7909 SspI AAT'ATT Cuts at: 5 52 5833 5 Size: 47 5781 2081 Fragments arranged by size: 5781 2081 47 StuI AGG'CCT Cuts at: 1517 4139 1517 Size: 2622 5287 StyI C'CwwG_G Cuts at: 513 1065 2022 2160 2265 4140 4623 5348 513 Size: 552 957 138 105 1875 483 725 3074 Fragments arranged by size: 3074 1875 957 725 552 483 138 105 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4851 5050 5936 5953 6106 6291 6736 Size: 1255 3271 199 886 17 153 185 445 Cuts at: 6736 325 Size: 1498 Fragments arranged by size: 3271 1498 1255 886 445 199 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5190 6155 7867 Size: 274 80 33 51 1443 3213 965 1712 Cuts at: 7867 96 Size: 138 Fragments arranged by size: 3213 1712 1443 965 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7869 7901 7869 Size: 32 7877 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7909 XbaI T'CTAG_A Cuts at: 3802 5245 3802 Size: 1443 6466 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2473 2473 Size: 7909 XhoI C'TCGA_G Cuts at: 1943 3998 1943 Size: 2055 5854 XmaI C'CCGG_G Cuts at: 1870 4381 4885 5234 1870 Size: 2511 504 349 4545 Fragments arranged by size: 4545 2511 504 349 XmnI GAAnn'nnTTC Cuts at: 2310 3249 6038 2310 Size: 939 2789 4181 Fragments arranged by size: 4181 2789 939 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5717 276 Size: 53 83 186 936 4183 2468 Fragments arranged by size: 4183 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I EcoRV FseI FspAI NruI NsiI PacI PsrI RsrII SacII SanDI SapI SexAI SfiI SwaI