Restriction map for
pFN21ASDA1274
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# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII AccI AclI AflII Alw44I
AocI ApaLI AscI AseI AsnI
Asp700I AviII AvrII BamHI BbeI
BbrPI BfrI BglII BlnI BsiWI
BsmBI BsmI BspDI BspLU11I BsrDI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
EagI EclXI Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HincII HindII HindIII HpaI KasI
KspI MfeI MunI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
StuI SwaI XbaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 794 GGTACC G^GTACC
AccIII 623 TCCGGA T^CCGGA
AcsI 2113 GAATTT [GA]^AATT[CT]
AgeI 2513 ACCGGT A^CCGGT
ApoI 2113 GAATTT [GA]^AATT[CT]
Asp718I 794 GGTACC G^GTACC
AspI 2191 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 1983 CCTCAGC CCTCAGC(-5/-2)
BciVI 1202 GTATCC GTATCC(6/5)
BclI 179 TGATCA T^GATCA
BfaI 122 CTAG C^TAG
BsaAI 183 CACGTA [CT]AC^GT[GA]
BsaBI 2238 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 623 TCCGGA T^CCGGA
BsiEI 1207 CGACCG CG[GA][CT]^CG
Bsp1407I 758 TGTACA T^GTACA
BspEI 623 TCCGGA T^CCGGA
BsrBI 2478 GAGCGG CCGCTC(-3/-3)
BsrGI 758 TGTACA T^GTACA
BssSI 2077 CTCGTG CACGAG(-5/-1)
BstAPI 2283 GCAGGAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 2158 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
FbaI 179 TGATCA T^GATCA
KpnI 794 GGTACC GGTAC^C
MaeI 122 CTAG C^TAG
MamI 2238 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 2354 ACGCGT A^CGCGT
MroI 623 TCCGGA T^CCGGA
NaeI 248 GCCGGC GCC^GGC
NcoI 723 CCATGG C^CATGG
NgoMIV 248 GCCGGC G^CCGGC
PflFI 2191 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2513 ACCGGT A^CCGGT
ScaI 2318 AGTACT AGT^ACT
SexAI 278 ACCTGGT A^CC[AT]GGT
SfiI 1929 GGCCCTGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 589 CCCGGG CCC^GGG
SphI 1532 GCATGC GCATG^C
SspBI 758 TGTACA T^GTACA
TfiI 2343 GATTC G^A[AT]TC
Tsp509I 2114 AATT ^AATT
Tth111I 2191 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 589 CCCGGG C^CCGGG
XmaI 589 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 1479 CGCG CG^CG
2355 CGCG
AflIII 239 ACACGT A^C[GA][CT]GT
2354 ACGCGT
Aor51HI 2184 AGCGCT AGC^GCT
2327 AGCGCT
BbsI 925 GTCTTC GAAGAC(2/6)
1825 GTCTTC
BcgI 2541 CGAGCCCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1961 GCACTGGCTTCG
BpuAI 925 GTCTTC GAAGAC(2/6)
1825 GTCTTC
BspHI 564 TCATGA T^CATGA
1314 TCATGA
BsrFI 248 GCCGGC [GA]^CCGG[CT]
2513 ACCGGT
BsrI 1963 ACTGG ACTGG(1/-1)
840 CCAGT
BstUI 1479 CGCG CG^CG
2355 CGCG
Cfr10I 248 GCCGGC [GA]^CCGG[CT]
2513 ACCGGT
DrdI 847 GACGCCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1459 GACGATCTGGTC
Eco47III 2184 AGCGCT AGC^GCT
2327 AGCGCT
MaeII 184 ACGT A^CGT
241 ACGT
MseI 1301 TTAA T^TAA
2450 TTAA
MvnI 1479 CGCG CG^CG
2355 CGCG
NspI 741 GCATGT [GA]CATG^[CT]
1532 GCATGC
PaeR7I 545 CTCGAG C^TCGAG
2539 CTCGAG
PleI 2380 GACTC GAGTC(4/5)
825 GAGTC
RcaI 564 TCATGA T^CATGA
1314 TCATGA
SacI 1401 GAGCTC GAGCT^C
2074 GAGCTC
Tru9I 1301 TTAA T^TAA
2450 TTAA
XcmI 956 CCAACCTGGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2003 CCACAACTGCGATGG
XhoI 545 CTCGAG C^TCGAG
2539 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AciI AcyI AfaI AhdI AluI
AlwI AlwNI ApaI AspEI AspHI
AvaI AvaII BalI BanI BanII
BbvI BcnI BglI BlpI BmyI
BpmI Bpu1102I BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BssKI BstF5I BstNI BstXI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
Eam1105I EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflMI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TseI Tsp45I TspRI TthHB8I Van91I
XhoII