Restriction map for
pFN21ASDA1274
(Circular) (Six-base) MAPSORT of: pFN21ASDA1274.seq Check: 7468 from: 1 to: 7297
pFN21ASDA1274 7297 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2386 4260 2386
Size: 1874 5423
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5107 278
Size: 53 83 186 936 3571 2468
Fragments arranged by size:
3571 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4640 1937
Size: 2703 4594
Acc65I G'GTAC_C
Cuts at: 2817 4618 2817
Size: 1801 5496
AclI AA'CG_TT
Cuts at: 1563 5424 5797 1563
Size: 3861 373 3063
Fragments arranged by size:
3861 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2935 3785 3835 4405 5360 6744 7012 1632
Size: 1303 850 50 570 955 1384 268 1917
Fragments arranged by size:
1917 1384 1303 955 850 570 268 50
AfeI AGC'GCT
Cuts at: 4209 4352 4209
Size: 143 7154
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7100
AflIII A'CryG_T
Cuts at: 1203 2262 4377 6196 7173 1203
Size: 1059 2115 1819 977 1327
Fragments arranged by size:
2115 1819 1327 1059 977
AgeI A'CCGG_T
Cuts at: 4536 6128 4536
Size: 1592 5705
AhdI GACnn_n'nnGTC
Cuts at: 2554 2615 3269 6026 2554
Size: 61 654 2757 3825
Fragments arranged by size:
3825 2757 654 61
AleI CACnn'nnGTG
Cuts at: 1166 3021 1166
Size: 1855 5442
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2165 3917 4448 1184
Size: 981 1752 531 4033
Fragments arranged by size:
4033 1752 981 531
AlwNI CAG_nnn'CTG
Cuts at: 3047 3273 3378 3437 3870 3946 3986 4428 5040
Size: 226 105 59 433 76 40 442 612
Cuts at: 5040 6612 3047
Size: 1572 3732
Fragments arranged by size:
3732 1572 612 442 433 226 105 76
59 40
ApaI G_GGCC'C
Cuts at: 2383 3723 3806 2383
Size: 1340 83 5874
Fragments arranged by size:
5874 1340 83
ApaLI G'TGCA_C
Cuts at: 5354 6510 5354
Size: 1156 6141
ApoI r'AATT_y
Cuts at: 1493 4136 4606 4832 4984 7093 1493
Size: 2643 470 226 152 2109 1697
Fragments arranged by size:
2643 2109 1697 470 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5851 160
Size: 872 4819 1606
Fragments arranged by size:
4819 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7297
AvaI C'yCGr_G
Cuts at: 1870 1943 2568 2612 2837 3921 4079 4490 4562
Size: 73 625 44 225 1084 158 411 72
Cuts at: 4562 4577 4622 1870
Size: 15 45 4545
Fragments arranged by size:
4545 1084 625 411 225 158 73 72
45 44 15
BamHI G'GATC_C
Cuts at: 4627 4994 4627
Size: 367 6930
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2066 2360 2817 2999 3115
Size: 325 217 627 279 294 457 182 116
Cuts at: 3115 3547 4179 4618 6073 618
Size: 432 632 439 1455 1842
Fragments arranged by size:
1842 1455 632 627 457 439 432 325
294 279 217 182 116
BanII G_rGCy'C
Cuts at: 1391 2383 2412 2730 3242 3428 3470 3723 3806
Size: 992 29 318 512 186 42 253 83
Cuts at: 3806 4101 4569 4616 1391
Size: 295 468 47 4072
Fragments arranged by size:
4072 992 512 468 318 295 253 186
83 47 42 29
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2941 3841 928
Size: 971 1042 900 4384
Fragments arranged by size:
4384 1042 971 900
BbvCI CC'TCA_GC
Cuts at: 4007 4007
Size: 7297
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3973 4007 4553 4587 5488 5522 1903
Size: 34 2036 34 546 34 901 34 3678
Fragments arranged by size:
3678 2036 901 546 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3236 5189 6399 3236
Size: 1953 1210 4134
Fragments arranged by size:
4134 1953 1210
BclI T'GATC_A
Cuts at: 1548 2202 1548
Size: 654 6643
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2088 2964 3959
Size: 107 122 71 631 605 416 876 995
Cuts at: 3959 4011 5908 136
Size: 52 1897 1525
Fragments arranged by size:
1897 1525 995 876 631 605 416 122
107 71 52
BglII A'GATC_T
Cuts at: 7292 7292
Size: 7297
BlpI GC'TnA_GC
Cuts at: 2675 3422 3427 3506 4714 2675
Size: 747 5 79 1208 5258
Fragments arranged by size:
5258 1208 747 79 5
Bme1580I G_kGCm'C
Cuts at: 1790 2363 2383 3002 3120 3391 3552 3696 3723
Size: 573 20 619 118 271 161 144 27
Cuts at: 3723 3806 3919 4182 5358 6514 1790
Size: 83 113 263 1176 1156 2573
Fragments arranged by size:
2573 1176 1156 619 573 271 263 161
144 118 113 83 27 20
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7297
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2857 5028 5986
Size: 416 53 83 399 24 1440 2171 958
Cuts at: 5986 442
Size: 1753
Fragments arranged by size:
2171 1753 1440 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7297
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2447 2456 2582 2654 2876 3317 3764 3773
Size: 1208 9 126 72 222 441 447 9
Cuts at: 3773 3968 4412 4507 4517 5957 1239
Size: 195 444 95 10 1440 2579
Fragments arranged by size:
2579 1440 1208 447 444 441 222 195
126 95 72 10 9 9
Bpu10I CC'TnA_GC
Cuts at: 2292 3396 4007 2292
Size: 1104 611 5582
Fragments arranged by size:
5582 1104 611
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2234 5422 6287 6585 2234
Size: 3188 865 298 2946
Fragments arranged by size:
3188 2946 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2062 2888 3496 3740 4220 5960 882
Size: 617 563 826 608 244 480 1740 2219
Fragments arranged by size:
2219 1740 826 617 608 563 480 244
BsaAI yAC'GTr
Cuts at: 493 1204 2208 4594 5088 493
Size: 711 1004 2386 494 2702
Fragments arranged by size:
2702 2386 1004 711 494
BsaBI GATnn'nnATC
Cuts at: 4265 7291 4265
Size: 3026 4271
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2871 3624 4235 5104
Size: 53 83 186 936 1338 753 611 869
Cuts at: 5104 5486 6121 275
Size: 382 635 1451
Fragments arranged by size:
1451 1338 936 869 753 635 611 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2646 3232 4536 5730 6128 6402 6549 6960 2646
Size: 586 1304 1194 398 274 147 411 2983
Fragments arranged by size:
2983 1304 1194 586 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2765 2795 2849 2879 3662
Size: 30 165 30 1362 30 54 30 783
Cuts at: 3662 3692 1178
Size: 30 4783
Fragments arranged by size:
4783 1362 783 165 54 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2237 2789 3646 3683 3941 4106 4109 1186
Size: 1051 552 857 37 258 165 3 4374
Fragments arranged by size:
4374 1051 857 552 258 165 37 3
BseYI C'CCAG_C
Cuts at: 2046 2356 2435 2670 2900 2938 3036 3241 3576
Size: 310 79 235 230 38 98 205 335
Cuts at: 3576 3581 3759 4480 6500 6996 2046
Size: 5 178 721 2020 496 2347
Fragments arranged by size:
2347 2020 721 496 335 310 235 230
205 178 98 79 38 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2420 2559 4181 4255 1163
Size: 573 684 139 1622 74 4205
Fragments arranged by size:
4205 1622 684 573 139 74
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3233 4747 5508 6536 1540
Size: 275 186 1232 1514 761 1028 2301
Fragments arranged by size:
2301 1514 1232 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2144 2298 3428 4101 5358 5443 6514 2144
Size: 154 1130 673 1257 85 1071 2927
Fragments arranged by size:
2927 1257 1130 1071 673 154 85
BsmI GAATG_Cn'
Cuts at: 4808 4901 4808
Size: 93 7204
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2144 2298 2363 2383 2412 2730 3002
Size: 399 354 154 65 20 29 318 272
Cuts at: 3002 3120 3242 3391 3428 3470 3552 3696 3723
Size: 118 122 149 37 42 82 144 27
Cuts at: 3723 3806 3919 4101 4182 4569 4616 5358 5443
Size: 83 113 182 81 387 47 742 85
Cuts at: 5443 6514 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 387 354 318 272
182 154 149 144 122 118 113 85
83 82 81 65 47 42 37 29
27 20
BspEI T'CCGG_A
Cuts at: 2646 6960 2646
Size: 4314 2983
BspHI T'CATG_A
Cuts at: 2587 3337 5186 6916 2587
Size: 750 1849 1730 2968
Fragments arranged by size:
2968 1849 1730 750
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2386 3836 4260 4652 844
Size: 1542 1450 424 392 3489
Fragments arranged by size:
3489 1542 1450 424 392
BsrBI CCG'CTC
Cuts at: 1394 1823 4503 5184 1394
Size: 429 2680 681 3507
Fragments arranged by size:
3507 2680 681 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5792 5966 65
Size: 1183 4544 174 1396
Fragments arranged by size:
4544 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2271 4536 5941 6128 1700
Size: 110 461 2265 1405 187 2869
Fragments arranged by size:
2869 2265 1405 461 187 110
BsrGI T'GTAC_A
Cuts at: 96 2781 96
Size: 2685 4612
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7297
BssSI C'ACGA_G
Cuts at: 4100 5357 6369 4100
Size: 1257 1012 5028
Fragments arranged by size:
5028 1257 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4312 794
Size: 3518 3779
BstBI TT'CG_AA
Cuts at: 2007 6101 2007
Size: 4094 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7297
BstXI CCAn_nnnn'nTGG
Cuts at: 2188 2606 3595 2188
Size: 418 989 5890
Fragments arranged by size:
5890 989 418
BstYI r'GATC_y
Cuts at: 1972 2232 2655 4467 4627 4994 5379 5396 6848
Size: 260 423 1812 160 367 385 17 1452
Cuts at: 6848 6934 6946 7292 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1812 1452 423 385 367 346 260
160 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2364 2746 2926 513
Size: 552 957 342 382 180 4884
Fragments arranged by size:
4884 957 552 382 342 180
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3442 4049 487
Size: 683 56 2216 607 3735
Fragments arranged by size:
3735 2216 683 607 56
BtsI GCAGTG_nn'
Cuts at: 2098 3028 3159 4813 5607 5627 2098
Size: 930 131 1654 794 20 3768
Fragments arranged by size:
3768 1654 930 794 131 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7297
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7262
DraI TTT'AAA
Cuts at: 4601 4601
Size: 7297
DraIII CAC_nnn'GTG
Cuts at: 4186 4186
Size: 7297
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2876 3488 6304 809
Size: 507 1560 612 2816 1802
Fragments arranged by size:
2816 1802 1560 612 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2034 2640 2739
Size: 54 1425 50 275 18 204 606 99
Cuts at: 2739 3531 4012 4744 5633 7262 8
Size: 792 481 732 889 1629 43
Fragments arranged by size:
1629 1425 889 792 732 606 481 275
204 99 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4744 1537
Size: 275 2932 4090
Fragments arranged by size:
4090 2932 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2801 3135 4376 5227 1353
Size: 1448 334 1241 851 3423
Fragments arranged by size:
3423 1448 1241 851 334
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5884 6258 6404 387
Size: 1064 138 4295 374 146 1280
Fragments arranged by size:
4295 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3426 4099 3426
Size: 673 6624
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2447 2456 2582 2654 2876 2935 3317
Size: 393 815 9 126 72 222 59 382
Cuts at: 3317 3764 3773 3785 3835 3968 4405 4412 4507
Size: 447 9 12 50 133 437 7 95
Cuts at: 4507 4517 5360 5957 6744 7012 1239
Size: 10 843 597 787 268 1524
Fragments arranged by size:
1524 843 815 787 597 447 437 393
382 268 222 133 126 95 72 59
50 12 10 9 9 7
EcoNI CCTnn'n_nnAGG
Cuts at: 3476 3665 3789 3971 4394 4411 4522 3476
Size: 189 124 182 423 17 111 6251
Fragments arranged by size:
6251 423 189 182 124 111 17
EcoO109I rG'GnC_Cy
Cuts at: 2080 2379 2380 2458 2602 2713 3434 3451 3592
Size: 299 1 78 144 111 721 17 141
Cuts at: 3592 3720 3802 3803 3867 3952 3976 4416 2080
Size: 128 82 1 64 85 24 440 4961
Fragments arranged by size:
4961 721 440 299 144 141 128 111
85 82 78 64 24 17 1 1
EcoRI G'AATT_C
Cuts at: 4606 4984 4606
Size: 378 6919
FspI TGC'GCA
Cuts at: 5803 5803
Size: 7297
HaeII r_GCGC'y
Cuts at: 1678 3287 3588 4211 4354 6444 1678
Size: 1609 301 623 143 2090 2531
Fragments arranged by size:
2531 2090 1609 623 301 143
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2092 2124 2540 2572 2601
Size: 32 135 32 520 32 416 32 29
Cuts at: 2601 2633 3255 3287 3476 3508 4430 4462 5938
Size: 32 622 32 189 32 922 32 1476
Cuts at: 5938 5970 6012 6044 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1476 922 622 520 416 189 135
42 32 32 32 32 32 32 32
32 32 32 29
HincII GTy'rAC
Cuts at: 669 1657 1938 4641 4887 669
Size: 988 281 2703 246 3079
Fragments arranged by size:
3079 2703 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7297
HpaI GTT'AAC
Cuts at: 4887 4887
Size: 7297
KpnI G_GTAC'C
Cuts at: 2821 4622 2821
Size: 1801 5496
MfeI C'AATT_G
Cuts at: 4896 4896
Size: 7297
MluI A'CGCG_T
Cuts at: 4377 7173 4377
Size: 2796 4501
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2847 3253 3660 6411 6595 1305
Size: 1542 406 407 2751 184 2007
Fragments arranged by size:
2751 2007 1542 407 406 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2642 2741 3533 10
Size: 54 1425 343 810 99 792 3774
Fragments arranged by size:
3774 1425 810 792 343 99 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3021 3899 4430 5255 5614 5773 518
Size: 648 1855 878 531 825 359 159 2042
Fragments arranged by size:
2042 1855 878 825 648 531 359 159
MspA1I CmG'CkG
Cuts at: 1606 2674 2955 3352 3378 3742 3763 3943 4002
Size: 1068 281 397 26 364 21 180 59
Cuts at: 4002 4011 4165 4327 4713 5390 6538 6783 1606
Size: 9 154 162 386 677 1148 245 2120
Fragments arranged by size:
2120 1148 1068 677 397 386 364 281
245 180 162 154 59 26 21 9
NaeI GCC'GGC
Cuts at: 2273 2273
Size: 7297
NcoI C'CATG_G
Cuts at: 513 1065 2022 2746 513
Size: 552 957 724 5064
Fragments arranged by size:
5064 957 724 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7297
NgoMIV G'CCGG_C
Cuts at: 2271 2271
Size: 7297
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7297
NotI GC'GGCC_GC
Cuts at: 4744 4744
Size: 7297
NspI r_CATG'y
Cuts at: 1132 1229 2768 3559 4655 6200 1132
Size: 97 1539 791 1096 1545 2229
Fragments arranged by size:
2229 1545 1539 1096 791 97
PasI CC'CwG_GG
Cuts at: 1348 3121 3247 3595 1348
Size: 1773 126 348 5050
Fragments arranged by size:
5050 1773 348 126
PciI A'CATG_T
Cuts at: 6196 6196
Size: 7297
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2737 3946 3986 1108
Size: 1629 1209 40 4419
Fragments arranged by size:
4419 1629 1209 40
PfoI T'CCnGG_A
Cuts at: 2889 2889
Size: 7297
PmeI GTTT'AAAC
Cuts at: 4601 4601
Size: 7297
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5366 5398 6879 6911 5366
Size: 32 1481 32 5752
Fragments arranged by size:
5752 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2458 2713 3451 3976 2458
Size: 255 738 525 5779
Fragments arranged by size:
5779 738 525 255
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7297
PsiI TTA'TAA
Cuts at: 4867 4867
Size: 7297
PspOMI G'GGCC_C
Cuts at: 2379 3719 3802 2379
Size: 1340 83 5874
Fragments arranged by size:
5874 1340 83
PspXI vC'TCGA_Gb
Cuts at: 4562 4562
Size: 7297
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2011 2043 2011
Size: 32 7265
PstI C_TGCA'G
Cuts at: 830 2805 2913 4326 4341 4559 4649 830
Size: 1975 108 1413 15 218 90 3478
Fragments arranged by size:
3478 1975 1413 218 108 90 15
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7297
PvuII CAG'CTG
Cuts at: 2674 2955 3742 3763 3943 4002 4165 4327 2674
Size: 281 787 21 180 59 163 162 5644
Fragments arranged by size:
5644 787 281 180 163 162 59 21
SacI G_AGCT'C
Cuts at: 3428 4101 3428
Size: 673 6624
SalI G'TCGA_C
Cuts at: 1936 4639 1936
Size: 2703 4594
SbfI CC_TGCA'GG
Cuts at: 4649 4649
Size: 7297
ScaI AGT'ACT
Cuts at: 4343 5545 7257 4343
Size: 1202 1712 4383
Fragments arranged by size:
4383 1712 1202
SexAI A'CCwGG_T
Cuts at: 2301 2301
Size: 7297
SfcI C'TryA_G
Cuts at: 826 1045 2084 2801 2812 2909 3700 3814 4322
Size: 219 1039 717 11 97 791 114 508
Cuts at: 4322 4337 4555 4645 5780 6461 6652 826
Size: 15 218 90 1135 681 191 1471
Fragments arranged by size:
1471 1135 1039 791 717 681 508 219
218 191 114 97 90 15 11
SfiI GGCCn_nnn'nGGCC
Cuts at: 3959 3959
Size: 7297
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7297
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7297
SmaI CCC'GGG
Cuts at: 1872 2614 4624 1872
Size: 742 2010 4545
Fragments arranged by size:
4545 2010 742
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2249 2568 4562 5401 6302 6564
Size: 197 926 306 319 1994 839 901 262
Cuts at: 6564 820
Size: 1553
Fragments arranged by size:
1994 1553 926 901 839 319 306 262
197
SnaBI TAC'GTA
Cuts at: 493 4594 5088 493
Size: 4101 494 2702
Fragments arranged by size:
4101 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7297
SphI G_CATG'C
Cuts at: 1132 3559 4655 1132
Size: 2427 1096 3774
Fragments arranged by size:
3774 2427 1096
SspI AAT'ATT
Cuts at: 5 52 5221 5
Size: 47 5169 2081
Fragments arranged by size:
5169 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7297
StyI C'CwwG_G
Cuts at: 513 1065 2022 2605 2746 3588 3972 4069 4736
Size: 552 957 583 141 842 384 97 667
Cuts at: 4736 513
Size: 3074
Fragments arranged by size:
3074 957 842 667 583 552 384 141
97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3643 4076 5324 5341 5494 5679 6124
Size: 1255 2063 433 1248 17 153 185 445
Cuts at: 6124 325
Size: 1498
Fragments arranged by size:
2063 1498 1255 1248 445 433 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2781 4341 5543
Size: 274 80 33 51 1443 804 1560 1202
Cuts at: 5543 7255 96
Size: 1712 138
Fragments arranged by size:
1712 1560 1443 1202 804 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7257 7289 7257
Size: 32 7265
Tth111I GACn'n_nGTC
Cuts at: 1316 4217 1316
Size: 2901 4396
XbaI T'CTAG_A
Cuts at: 4633 4633
Size: 7297
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2986 4033 2986
Size: 1047 6250
XhoI C'TCGA_G
Cuts at: 1943 2568 4562 1943
Size: 625 1994 4678
Fragments arranged by size:
4678 1994 625
XmaI C'CCGG_G
Cuts at: 1870 2612 4622 1870
Size: 742 2010 4545
Fragments arranged by size:
4545 2010 742
XmnI GAAnn'nnTTC
Cuts at: 5426 5426
Size: 7297
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5105 276
Size: 53 83 186 936 3571 2468
Fragments arranged by size:
3571 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII SacI SalI SbfI
ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PmlI RsrII SacII SanDI
SapI SfoI SrfI SwaI