Restriction map for
pFN21ASDA1274
(Linear) (Six-base) MAPSORT of: KIAA1274.seq Check: 951 from: 1 to: 2568
KIAA1274.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 363 2237 2568
Size: 363 1874 331
Fragments arranged by size:
1874 363 331
Acc65I G'GTAC_C
Cuts at: 0 794 2568
Size: 794 1774
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 912 1762 1812 2382 2568
Size: 912 850 50 570 186
Fragments arranged by size:
912 850 570 186 50
AfeI AGC'GCT
Cuts at: 0 2186 2329 2568
Size: 2186 143 239
Fragments arranged by size:
2186 239 143
AflIII A'CryG_T
Cuts at: 0 239 2354 2568
Size: 239 2115 214
Fragments arranged by size:
2115 239 214
AgeI A'CCGG_T
Cuts at: 0 2513 2568
Size: 2513 55
AhdI GACnn_n'nnGTC
Cuts at: 0 531 592 1246 2568
Size: 531 61 654 1322
Fragments arranged by size:
1322 654 531 61
AleI CACnn'nnGTG
Cuts at: 0 998 2568
Size: 998 1570
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 142 1894 2425 2568
Size: 142 1752 531 143
Fragments arranged by size:
1752 531 143 142
AlwNI CAG_nnn'CTG
Cuts at: 0 1024 1250 1355 1414 1847 1923 1963 2405
Size: 1024 226 105 59 433 76 40 442
Cuts at: 2405 2568
Size: 163
Fragments arranged by size:
1024 442 433 226 163 105 76 59
40
ApaI G_GGCC'C
Cuts at: 0 360 1700 1783 2568
Size: 360 1340 83 785
Fragments arranged by size:
1340 785 360 83
ApoI r'AATT_y
Cuts at: 0 2113 2568
Size: 2113 455
AvaI C'yCGr_G
Cuts at: 0 545 589 814 1898 2056 2467 2539 2554
Size: 545 44 225 1084 158 411 72 15
Cuts at: 2554 2568
Size: 14
Fragments arranged by size:
1084 545 411 225 158 72 44 15
14
BanI G'GyrC_C
Cuts at: 0 43 337 794 976 1092 1524 2156 2568
Size: 43 294 457 182 116 432 632 412
Fragments arranged by size:
632 457 432 412 294 182 116 43
BanII G_rGCy'C
Cuts at: 0 360 389 707 1219 1405 1447 1700 1783
Size: 360 29 318 512 186 42 253 83
Cuts at: 1783 2078 2546 2568
Size: 295 468 22
Fragments arranged by size:
512 468 360 318 295 253 186 83
42 29 22
BbsI GAAGACnn'nnnn_
Cuts at: 0 918 1818 2568
Size: 918 900 750
Fragments arranged by size:
918 900 750
BbvCI CC'TCA_GC
Cuts at: 0 1984 2568
Size: 1984 584
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1950 1984 2530 2564 2568
Size: 1950 34 546 34 4
Fragments arranged by size:
1950 546 34 34 4
BciVI GTATCCnnnnn_n'
Cuts at: 0 1213 2568
Size: 1213 1355
BclI T'GATC_A
Cuts at: 0 179 2568
Size: 179 2389
BglI GCCn_nnn'nGGC
Cuts at: 0 65 941 1936 1988 2568
Size: 65 876 995 52 580
Fragments arranged by size:
995 876 580 65 52
BlpI GC'TnA_GC
Cuts at: 0 652 1399 1404 1483 2568
Size: 652 747 5 79 1085
Fragments arranged by size:
1085 747 652 79 5
Bme1580I G_kGCm'C
Cuts at: 0 340 360 979 1097 1368 1529 1673 1700
Size: 340 20 619 118 271 161 144 27
Cuts at: 1700 1783 1896 2159 2568
Size: 83 113 263 409
Fragments arranged by size:
619 409 340 271 263 161 144 118
113 83 27 20
BmrI ACTGGGnnnn_n'
Cuts at: 0 834 2568
Size: 834 1734
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 433 559 631 853 1294 1741 1750
Size: 424 9 126 72 222 441 447 9
Cuts at: 1750 1945 2389 2484 2494 2568
Size: 195 444 95 10 74
Fragments arranged by size:
447 444 441 424 222 195 126 95
74 72 10 9 9
Bpu10I CC'TnA_GC
Cuts at: 0 269 1373 1984 2568
Size: 269 1104 611 584
Fragments arranged by size:
1104 611 584 269
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 2568
Size: 211 2357
BsaI GGTCTCn'nnnn_
Cuts at: 0 39 865 1473 1717 2197 2568
Size: 39 826 608 244 480 371
Fragments arranged by size:
826 608 480 371 244 39
BsaAI yAC'GTr
Cuts at: 0 185 2568
Size: 185 2383
BsaBI GATnn'nnATC
Cuts at: 0 2242 2568
Size: 2242 326
BsaHI Gr'CG_yC
Cuts at: 0 848 1601 2212 2568
Size: 848 753 611 356
Fragments arranged by size:
848 753 611 356
BsaWI w'CCGG_w
Cuts at: 0 623 1209 2513 2568
Size: 623 586 1304 55
Fragments arranged by size:
1304 623 586 55
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 742 772 826 856 1639 1669 2568
Size: 742 30 54 30 783 30 899
Fragments arranged by size:
899 783 742 54 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 214 766 1623 1660 1918 2083 2086 2568
Size: 214 552 857 37 258 165 3 482
Fragments arranged by size:
857 552 482 258 214 165 37 3
BseYI C'CCAG_C
Cuts at: 0 23 333 412 647 877 915 1013 1218
Size: 23 310 79 235 230 38 98 205
Cuts at: 1218 1553 1558 1736 2457 2568
Size: 335 5 178 721 111
Fragments arranged by size:
721 335 310 235 230 205 178 111
98 79 38 23 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 536 2158 2232 2568
Size: 397 139 1622 74 336
Fragments arranged by size:
1622 397 336 139 74
BsiEI CG_ry'CG
Cuts at: 0 1210 2568
Size: 1210 1358
BsiHKAI G_wGCw'C
Cuts at: 0 121 275 1405 2078 2568
Size: 121 154 1130 673 490
Fragments arranged by size:
1130 673 490 154 121
Bsp1286I G_dGCh'C
Cuts at: 0 121 275 340 360 389 707 979 1097
Size: 121 154 65 20 29 318 272 118
Cuts at: 1097 1219 1368 1405 1447 1529 1673 1700 1783
Size: 122 149 37 42 82 144 27 83
Cuts at: 1783 1896 2078 2159 2546 2568
Size: 113 182 81 387 22
Fragments arranged by size:
387 318 272 182 154 149 144 122
121 118 113 83 82 81 65 42
37 29 27 22 20
BspEI T'CCGG_A
Cuts at: 0 623 2568
Size: 623 1945
BspHI T'CATG_A
Cuts at: 0 564 1314 2568
Size: 564 750 1254
Fragments arranged by size:
1254 750 564
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 363 1813 2237 2568
Size: 363 1450 424 331
Fragments arranged by size:
1450 424 363 331
BsrBI CCG'CTC
Cuts at: 0 2480 2568
Size: 2480 88
BsrFI r'CCGG_y
Cuts at: 0 248 2513 2568
Size: 248 2265 55
Fragments arranged by size:
2265 248 55
BsrGI T'GTAC_A
Cuts at: 0 758 2568
Size: 758 1810
BssSI C'ACGA_G
Cuts at: 0 2077 2568
Size: 2077 491
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2289 2568
Size: 2289 279
BstXI CCAn_nnnn'nTGG
Cuts at: 0 165 583 1572 2568
Size: 165 418 989 996
Fragments arranged by size:
996 989 418 165
BstYI r'GATC_y
Cuts at: 0 209 632 2444 2568
Size: 209 423 1812 124
Fragments arranged by size:
1812 423 209 124
BtgI C'CryG_G
Cuts at: 0 341 723 903 2568
Size: 341 382 180 1665
Fragments arranged by size:
1665 382 341 180
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1419 2026 2568
Size: 1419 607 542
Fragments arranged by size:
1419 607 542
BtsI GCAGTG_nn'
Cuts at: 0 75 1005 1136 2568
Size: 75 930 131 1432
Fragments arranged by size:
1432 930 131 75
DraIII CAC_nnn'GTG
Cuts at: 0 2163 2568
Size: 2163 405
DrdI GACnn_nn'nnGTC
Cuts at: 0 853 1465 2568
Size: 853 612 1103
Fragments arranged by size:
1103 853 612
EaeI y'GGCC_r
Cuts at: 0 11 617 716 1508 1989 2568
Size: 11 606 99 792 481 579
Fragments arranged by size:
792 606 579 481 99 11
EarI CTCTTCn'nnn_
Cuts at: 0 778 1112 2353 2568
Size: 778 334 1241 215
Fragments arranged by size:
1241 778 334 215
EcoICRI GAG'CTC
Cuts at: 0 1403 2076 2568
Size: 1403 673 492
Fragments arranged by size:
1403 673 492
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 433 559 631 853 912 1294 1741
Size: 424 9 126 72 222 59 382 447
Cuts at: 1741 1750 1762 1812 1945 2382 2389 2484 2494
Size: 9 12 50 133 437 7 95 10
Cuts at: 2494 2568
Size: 74
Fragments arranged by size:
447 437 424 382 222 133 126 95
74 72 59 50 12 10 9 9
7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1453 1642 1766 1948 2371 2388 2499 2568
Size: 1453 189 124 182 423 17 111 69
Fragments arranged by size:
1453 423 189 182 124 111 69 17
EcoO109I rG'GnC_Cy
Cuts at: 0 57 356 357 435 579 690 1411 1428
Size: 57 299 1 78 144 111 721 17
Cuts at: 1428 1569 1697 1779 1780 1844 1929 1953 2393
Size: 141 128 82 1 64 85 24 440
Cuts at: 2393 2568
Size: 175
Fragments arranged by size:
721 440 299 175 144 141 128 111
85 82 78 64 57 24 17 1
1
HaeII r_GCGC'y
Cuts at: 0 1264 1565 2188 2331 2568
Size: 1264 301 623 143 237
Fragments arranged by size:
1264 623 301 237 143
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 69 101 517 549 578 610 1232 1264
Size: 69 32 416 32 29 32 622 32
Cuts at: 1264 1453 1485 2407 2439 2568
Size: 189 32 922 32 129
Fragments arranged by size:
922 622 416 189 129 69 32 32
32 32 32 32 29
KpnI G_GTAC'C
Cuts at: 0 798 2568
Size: 798 1770
MluI A'CGCG_T
Cuts at: 0 2354 2568
Size: 2354 214
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 824 1230 1637 2568
Size: 824 406 407 931
Fragments arranged by size:
931 824 407 406
MscI TGG'CCA
Cuts at: 0 619 718 1510 2568
Size: 619 99 792 1058
Fragments arranged by size:
1058 792 619 99
MslI CAynn'nnrTG
Cuts at: 0 998 1876 2407 2568
Size: 998 878 531 161
Fragments arranged by size:
998 878 531 161
MspA1I CmG'CkG
Cuts at: 0 651 932 1329 1355 1719 1740 1920 1979
Size: 651 281 397 26 364 21 180 59
Cuts at: 1979 1988 2142 2304 2568
Size: 9 154 162 264
Fragments arranged by size:
651 397 364 281 264 180 162 154
59 26 21 9
NaeI GCC'GGC
Cuts at: 0 250 2568
Size: 250 2318
NcoI C'CATG_G
Cuts at: 0 723 2568
Size: 723 1845
NgoMIV G'CCGG_C
Cuts at: 0 248 2568
Size: 248 2320
NspI r_CATG'y
Cuts at: 0 745 1536 2568
Size: 745 791 1032
Fragments arranged by size:
1032 791 745
PasI CC'CwG_GG
Cuts at: 0 1098 1224 1572 2568
Size: 1098 126 348 996
Fragments arranged by size:
1098 996 348 126
PflMI CCAn_nnn'nTGG
Cuts at: 0 714 1923 1963 2568
Size: 714 1209 40 605
Fragments arranged by size:
1209 714 605 40
PfoI T'CCnGG_A
Cuts at: 0 866 2568
Size: 866 1702
PpuMI rG'GwC_Cy
Cuts at: 0 435 690 1428 1953 2568
Size: 435 255 738 525 615
Fragments arranged by size:
738 615 525 435 255
PspOMI G'GGCC_C
Cuts at: 0 356 1696 1779 2568
Size: 356 1340 83 789
Fragments arranged by size:
1340 789 356 83
PspXI vC'TCGA_Gb
Cuts at: 0 2539 2568
Size: 2539 29
PstI C_TGCA'G
Cuts at: 0 782 890 2303 2318 2536 2568
Size: 782 108 1413 15 218 32
Fragments arranged by size:
1413 782 218 108 32 15
PvuII CAG'CTG
Cuts at: 0 651 932 1719 1740 1920 1979 2142 2304
Size: 651 281 787 21 180 59 163 162
Cuts at: 2304 2568
Size: 264
Fragments arranged by size:
787 651 281 264 180 163 162 59
21
SacI G_AGCT'C
Cuts at: 0 1405 2078 2568
Size: 1405 673 490
Fragments arranged by size:
1405 673 490
ScaI AGT'ACT
Cuts at: 0 2320 2568
Size: 2320 248
SexAI A'CCwGG_T
Cuts at: 0 278 2568
Size: 278 2290
SfcI C'TryA_G
Cuts at: 0 61 778 789 886 1677 1791 2299 2314
Size: 61 717 11 97 791 114 508 15
Cuts at: 2314 2532 2568
Size: 218 36
Fragments arranged by size:
791 717 508 218 114 97 61 36
15 11
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1936 2568
Size: 1936 632
SmaI CCC'GGG
Cuts at: 0 591 2568
Size: 591 1977
SmlI C'TyrA_G
Cuts at: 0 226 545 2539 2568
Size: 226 319 1994 29
Fragments arranged by size:
1994 319 226 29
SphI G_CATG'C
Cuts at: 0 1536 2568
Size: 1536 1032
StyI C'CwwG_G
Cuts at: 0 582 723 1565 1949 2046 2568
Size: 582 141 842 384 97 522
Fragments arranged by size:
842 582 522 384 141 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1620 2053 2568
Size: 1620 433 515
Fragments arranged by size:
1620 515 433
TatI w'GTAC_w
Cuts at: 0 758 2318 2568
Size: 758 1560 250
Fragments arranged by size:
1560 758 250
Tth111I GACn'n_nGTC
Cuts at: 0 2194 2568
Size: 2194 374
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 963 2010 2568
Size: 963 1047 558
Fragments arranged by size:
1047 963 558
XhoI C'TCGA_G
Cuts at: 0 545 2539 2568
Size: 545 1994 29
Fragments arranged by size:
1994 545 29
XmaI C'CCGG_G
Cuts at: 0 589 2568
Size: 589 1979
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflIII AgeI AhdI AleI
AlfI AlwNI ApaI ApoI AvaI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BlpI Bme1580I BmrI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrFI BsrGI BssSI BstAPI BstXI
BstYI BtgI BtgZI BtsI DraIII DrdI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KpnI MluI
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI
PasI PflMI PfoI PpuMI PspOMI PspXI PstI PvuII
SacI ScaI SexAI SfcI SfiI SmaI SmlI SphI
StyI TaqII TatI Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI AclI AflII AloI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BfrBI BglII BmgBI
BmtI BplI BsiWI BsmI BsmBI BsrDI BssHII BstBI
BstEII BstZ17I Bsu36I ClaI CspCI DraI EagI EciI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KasI MfeI NarI NdeI NheI NotI NruI
NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
SfoI SgfI SgrAI SnaBI SpeI SrfI SspI StuI
SwaI TstI XbaI XmnI ZraI