Restriction map for
pFN21ASDA1267
(Linear) (Six-base) MAPSORT of: KIAA1267.seq Check: 3531 from: 1 to: 3315
KIAA1267.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AccI GT'mk_AC
Cuts at: 0 1603 3315
Size: 1603 1712
Acc65I G'GTAC_C
Cuts at: 0 1452 3315
Size: 1452 1863
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 288 1070 1073 1339 1639 2668 2713
Size: 59 229 782 3 266 300 1029 45
Cuts at: 2713 3315
Size: 602
Fragments arranged by size:
1029 782 602 300 266 229 59 45
3
AfeI AGC'GCT
Cuts at: 0 910 2308 3315
Size: 910 1398 1007
Fragments arranged by size:
1398 1007 910
AflII C'TTAA_G
Cuts at: 0 2113 3315
Size: 2113 1202
AflIII A'CryG_T
Cuts at: 0 1777 3315
Size: 1777 1538
AleI CACnn'nnGTG
Cuts at: 0 1762 2809 3010 3315
Size: 1762 1047 201 305
Fragments arranged by size:
1762 1047 305 201
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3201 3315
Size: 3201 114
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3077 3109 3315
Size: 3077 32 206
Fragments arranged by size:
3077 206 32
AlwNI CAG_nnn'CTG
Cuts at: 0 37 1343 2691 3315
Size: 37 1306 1348 624
Fragments arranged by size:
1348 1306 624 37
ApoI r'AATT_y
Cuts at: 0 1385 1940 3315
Size: 1385 555 1375
Fragments arranged by size:
1385 1375 555
AvaI C'yCGr_G
Cuts at: 0 1267 3017 3315
Size: 1267 1750 298
Fragments arranged by size:
1750 1267 298
AvrII C'CTAG_G
Cuts at: 0 2965 3315
Size: 2965 350
BamHI G'GATC_C
Cuts at: 0 171 3315
Size: 171 3144
BanI G'GyrC_C
Cuts at: 0 12 1452 1567 1756 3315
Size: 12 1440 115 189 1559
Fragments arranged by size:
1559 1440 189 115 12
BanII G_rGCy'C
Cuts at: 0 546 2575 2974 3315
Size: 546 2029 399 341
Fragments arranged by size:
2029 546 399 341
BbeI G_GCGC'C
Cuts at: 0 16 3315
Size: 16 3299
BbsI GAAGACnn'nnnn_
Cuts at: 0 215 294 803 985 1513 3315
Size: 215 79 509 182 528 1802
Fragments arranged by size:
1802 528 509 215 182 79
BbvCI CC'TCA_GC
Cuts at: 0 1700 3315
Size: 1700 1615
BciVI GTATCCnnnnn_n'
Cuts at: 0 3042 3315
Size: 3042 273
BclI T'GATC_A
Cuts at: 0 504 3315
Size: 504 2811
BglI GCCn_nnn'nGGC
Cuts at: 0 2667 3315
Size: 2667 648
BlpI GC'TnA_GC
Cuts at: 0 365 3296 3315
Size: 365 2931 19
Fragments arranged by size:
2931 365 19
Bme1580I G_kGCm'C
Cuts at: 0 894 1572 2273 2790 3315
Size: 894 678 701 517 525
Fragments arranged by size:
894 701 678 525 517
BmgBI CAC'GTC
Cuts at: 0 1780 3315
Size: 1780 1535
BmrI ACTGGGnnnn_n'
Cuts at: 0 1971 3315
Size: 1971 1344
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 744 3315
Size: 744 2571
Bpu10I CC'TnA_GC
Cuts at: 0 1700 3315
Size: 1700 1615
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1032 1080 1191 3247 3315
Size: 1032 48 111 2056 68
Fragments arranged by size:
2056 1032 111 68 48
BsaI GGTCTCn'nnnn_
Cuts at: 0 1008 1072 2360 3315
Size: 1008 64 1288 955
Fragments arranged by size:
1288 1008 955 64
BsaAI yAC'GTr
Cuts at: 0 1420 3315
Size: 1420 1895
BsaBI GATnn'nnATC
Cuts at: 0 588 1886 3315
Size: 588 1298 1429
Fragments arranged by size:
1429 1298 588
BsaHI Gr'CG_yC
Cuts at: 0 13 3315
Size: 13 3302
BsaWI w'CCGG_w
Cuts at: 0 53 1853 3315
Size: 53 1800 1462
Fragments arranged by size:
1800 1462 53
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1917 1947 3231 3261 3315
Size: 1917 30 1284 30 54
Fragments arranged by size:
1917 1284 54 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2475 2571 2725 2788 3315
Size: 2475 96 154 63 527
Fragments arranged by size:
2475 527 154 96 63
BseYI C'CCAG_C
Cuts at: 0 175 960 2662 2819 2870 2874 2900 3072
Size: 175 785 1702 157 51 4 26 172
Cuts at: 3072 3315
Size: 243
Fragments arranged by size:
1702 785 243 175 172 157 51 26
4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1197 2483 2974 3171 3315
Size: 1197 1286 491 197 144
Fragments arranged by size:
1286 1197 491 197 144
BsiEI CG_ry'CG
Cuts at: 0 2859 3315
Size: 2859 456
BsiHKAI G_wGCw'C
Cuts at: 0 1623 2127 2575 3315
Size: 1623 504 448 740
Fragments arranged by size:
1623 740 504 448
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1284 1743 3315
Size: 1284 459 1572
Fragments arranged by size:
1572 1284 459
Bsp1286I G_dGCh'C
Cuts at: 0 546 894 1572 1623 2127 2273 2575 2790
Size: 546 348 678 51 504 146 302 215
Cuts at: 2790 2974 3315
Size: 184 341
Fragments arranged by size:
678 546 504 348 341 302 215 184
146 51
BspHI T'CATG_A
Cuts at: 0 507 3315
Size: 507 2808
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 924 1286 2825 3315
Size: 924 362 1539 490
Fragments arranged by size:
1539 924 490 362
BsrBI CCG'CTC
Cuts at: 0 19 3108 3315
Size: 19 3089 207
Fragments arranged by size:
3089 207 19
BsrDI GCAATG_nn'
Cuts at: 0 158 3315
Size: 158 3157
BsrFI r'CCGG_y
Cuts at: 0 717 3315
Size: 717 2598
BssSI C'ACGA_G
Cuts at: 0 2805 3315
Size: 2805 510
BstYI r'GATC_y
Cuts at: 0 171 583 3315
Size: 171 412 2732
Fragments arranged by size:
2732 412 171
Bsu36I CC'TnA_GG
Cuts at: 0 3201 3315
Size: 3201 114
BtgI C'CryG_G
Cuts at: 0 707 1018 1563 2273 2952 3315
Size: 707 311 545 710 679 363
Fragments arranged by size:
710 707 679 545 363 311
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 22 2522 3315
Size: 22 2500 793
Fragments arranged by size:
2500 793 22
BtsI GCAGTG_nn'
Cuts at: 0 102 680 1185 2886 3315
Size: 102 578 505 1701 429
Fragments arranged by size:
1701 578 505 429 102
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1596 1631 3315
Size: 1596 35 1684
Fragments arranged by size:
1684 1596 35
DraIII CAC_nnn'GTG
Cuts at: 0 1906 3315
Size: 1906 1409
EaeI y'GGCC_r
Cuts at: 0 2856 3216 3315
Size: 2856 360 99
Fragments arranged by size:
2856 360 99
EagI C'GGCC_G
Cuts at: 0 2856 3315
Size: 2856 459
EarI CTCTTCn'nnn_
Cuts at: 0 566 632 1235 1658 1746 2714 3315
Size: 566 66 603 423 88 968 601
Fragments arranged by size:
968 603 601 566 423 88 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1858 3126 3157 3315
Size: 1858 1268 31 158
Fragments arranged by size:
1858 1268 158 31
EcoICRI GAG'CTC
Cuts at: 0 2573 3315
Size: 2573 742
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 288 744 1070 1073 1339 1639 2668
Size: 59 229 456 326 3 266 300 1029
Cuts at: 2668 2713 3315
Size: 45 602
Fragments arranged by size:
1029 602 456 326 300 266 229 59
45 3
EcoO109I rG'GnC_Cy
Cuts at: 0 2234 2730 2837 3315
Size: 2234 496 107 478
Fragments arranged by size:
2234 496 478 107
EcoRI G'AATT_C
Cuts at: 0 1385 3315
Size: 1385 1930
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 228 3315
Size: 228 3087
FspI TGC'GCA
Cuts at: 0 1899 3315
Size: 1899 1416
HaeII r_GCGC'y
Cuts at: 0 16 912 2296 2310 3238 3303 3315
Size: 16 896 1384 14 928 65 12
Fragments arranged by size:
1384 928 896 65 16 14 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 247 279 748 780 1023 1055 1585 1617
Size: 247 32 469 32 243 32 530 32
Cuts at: 1617 1718 1750 2359 2391 2671 2703 2719 2751
Size: 101 32 609 32 280 32 16 32
Cuts at: 2751 3073 3105 3258 3290 3315
Size: 322 32 153 32 25
Fragments arranged by size:
609 530 469 322 280 247 243 153
101 32 32 32 32 32 32 32
32 32 32 25 16
KasI G'GCGC_C
Cuts at: 0 12 3315
Size: 12 3303
KpnI G_GTAC'C
Cuts at: 0 1456 3315
Size: 1456 1859
MfeI C'AATT_G
Cuts at: 0 618 3315
Size: 618 2697
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1943 1961 2762 3315
Size: 1943 18 801 553
Fragments arranged by size:
1943 801 553 18
MslI CAynn'nnrTG
Cuts at: 0 569 1762 2410 2602 2809 3010 3315
Size: 569 1193 648 192 207 201 305
Fragments arranged by size:
1193 648 569 305 207 201 192
MspA1I CmG'CkG
Cuts at: 0 37 960 1343 2666 2823 2874 3159 3185
Size: 37 923 383 1323 157 51 285 26
Cuts at: 3185 3315
Size: 130
Fragments arranged by size:
1323 923 383 285 157 130 51 37
26
NaeI GCC'GGC
Cuts at: 0 719 3315
Size: 719 2596
NarI GG'CG_CC
Cuts at: 0 13 3315
Size: 13 3302
NcoI C'CATG_G
Cuts at: 0 707 1563 2273 2952 3315
Size: 707 856 710 679 363
Fragments arranged by size:
856 710 707 679 363
NgoMIV G'CCGG_C
Cuts at: 0 717 3315
Size: 717 2598
NspI r_CATG'y
Cuts at: 0 2052 2764 3315
Size: 2052 712 551
Fragments arranged by size:
2052 712 551
PasI CC'CwG_GG
Cuts at: 0 3101 3315
Size: 3101 214
PpuMI rG'GwC_Cy
Cuts at: 0 2234 2730 2837 3315
Size: 2234 496 107 478
Fragments arranged by size:
2234 496 478 107
PshAI GACnn'nnGTC
Cuts at: 0 1296 3315
Size: 1296 2019
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 251 283 408 440 3315
Size: 251 32 125 32 2875
Fragments arranged by size:
2875 251 125 32 32
PstI C_TGCA'G
Cuts at: 0 168 1320 3315
Size: 168 1152 1995
Fragments arranged by size:
1995 1152 168
PvuII CAG'CTG
Cuts at: 0 37 960 2666 2874 3159 3315
Size: 37 923 1706 208 285 156
Fragments arranged by size:
1706 923 285 208 156 37
SacI G_AGCT'C
Cuts at: 0 2575 3315
Size: 2575 740
SapI GCTCTTCn'nnn_
Cuts at: 0 566 3315
Size: 566 2749
SexAI A'CCwGG_T
Cuts at: 0 747 3315
Size: 747 2568
SfcI C'TryA_G
Cuts at: 0 164 1316 2841 3315
Size: 164 1152 1525 474
Fragments arranged by size:
1525 1152 474 164
SfoI GGC'GCC
Cuts at: 0 14 3315
Size: 14 3301
SmlI C'TyrA_G
Cuts at: 0 1011 1059 1170 2113 3262 3315
Size: 1011 48 111 943 1149 53
Fragments arranged by size:
1149 1011 943 111 53 48
SphI G_CATG'C
Cuts at: 0 2052 2764 3315
Size: 2052 712 551
Fragments arranged by size:
2052 712 551
StyI C'CwwG_G
Cuts at: 0 203 707 1563 2273 2462 2952 2965 3315
Size: 203 504 856 710 189 490 13 350
Fragments arranged by size:
856 710 504 490 350 203 189 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 952 2142 2455 3315
Size: 952 1190 313 860
Fragments arranged by size:
1190 952 860 313
TatI w'GTAC_w
Cuts at: 0 1785 3315
Size: 1785 1530
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 35 67 1917 1949 2430 2462 2793 2825
Size: 35 32 1850 32 481 32 331 32
Cuts at: 2825 3231 3263 3315
Size: 406 32 52
Fragments arranged by size:
1850 481 406 331 52 35 32 32
32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 210 768 3069 3315
Size: 210 558 2301 246
Fragments arranged by size:
2301 558 246 210
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflII AflIII AleI AlfI
AloI AlwNI ApoI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglI BlpI Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I KasI KpnI MfeI MmeI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PpuMI
PshAI PsrI PstI PvuII SacI SapI SexAI SfcI
SfoI SmlI SphI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AclI AgeI AhdI ApaI ApaLI AscI
AseI AsiSI BaeI BcgI BfrBI BglII BmtI BplI
BsiWI BsmI BspEI BsrGI BssHII BstAPI BstBI BstEII
BstXI BstZ17I ClaI DraI DrdI EcoNI EcoRV FseI
FspAI HincII HindIII HpaI MluI MscI NdeI NheI
NotI NruI NsiI PacI PciI PflMI PfoI PmeI
PmlI PpiI PsiI PspOMI PspXI PvuI RsrII SacII
SalI SanDI SbfI ScaI SfiI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI StuI SwaI Tth111I XbaI
XhoI XmaI XmnI ZraI