(Circular) (Six-base) MAPSORT of: pFN21ASDA1263.seq Check: 3609 from: 1 to: 7267 pFN21ASDA1263 7267 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2987 2987 Size: 7267 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5077 278 Size: 53 83 186 936 3541 2468 Fragments arranged by size: 3541 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4610 1937 Size: 2673 4594 Acc65I G'GTAC_C Cuts at: 3704 4588 3704 Size: 884 6383 AclI AA'CG_TT Cuts at: 1563 5394 5767 1563 Size: 3831 373 3063 Fragments arranged by size: 3831 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4287 4539 5330 6714 6982 1632 Size: 2655 252 791 1384 268 1917 Fragments arranged by size: 2655 1917 1384 791 268 252 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7070 AflIII A'CryG_T Cuts at: 1203 3375 6166 7143 1203 Size: 2172 2791 977 1327 Fragments arranged by size: 2791 2172 1327 977 AgeI A'CCGG_T Cuts at: 6098 6098 Size: 7267 AhdI GACnn_n'nnGTC Cuts at: 5996 5996 Size: 7267 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7267 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2385 1184 Size: 1201 6066 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3495 3527 3495 Size: 32 7235 AlwNI CAG_nnn'CTG Cuts at: 2801 3644 5010 6582 2801 Size: 843 1366 1572 3486 Fragments arranged by size: 3486 1572 1366 843 ApaLI G'TGCA_C Cuts at: 2360 5324 6480 2360 Size: 2964 1156 3147 Fragments arranged by size: 3147 2964 1156 ApoI r'AATT_y Cuts at: 1493 2336 2729 3431 4576 4802 4954 7063 1493 Size: 843 393 702 1145 226 152 2109 1697 Fragments arranged by size: 2109 1697 1145 843 702 393 226 152 AseI AT'TA_AT Cuts at: 160 1032 2610 4146 5821 160 Size: 872 1578 1536 1675 1606 Fragments arranged by size: 1675 1606 1578 1536 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7267 AvaI C'yCGr_G Cuts at: 1870 1943 4592 1870 Size: 73 2649 4545 Fragments arranged by size: 4545 2649 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3241 3274 3241 Size: 33 7234 BamHI G'GATC_C Cuts at: 4597 4964 4597 Size: 367 6900 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3102 3704 4588 6043 618 Size: 325 217 627 1315 602 884 1455 1842 Fragments arranged by size: 1842 1455 1315 884 627 602 325 217 BanII G_rGCy'C Cuts at: 1391 4332 4586 1391 Size: 2941 254 4072 Fragments arranged by size: 4072 2941 254 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2245 2418 2910 3294 3318 3722 928 Size: 971 346 173 492 384 24 404 4473 Fragments arranged by size: 4473 971 492 404 384 346 173 24 BbvCI CC'TCA_GC Cuts at: 3129 3498 3129 Size: 369 6898 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5458 5492 1903 Size: 34 3521 34 3678 Fragments arranged by size: 3678 3521 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3066 5159 6369 3066 Size: 2093 1210 3964 Fragments arranged by size: 3964 2093 1210 BclI T'GATC_A Cuts at: 1548 3679 1548 Size: 2131 5136 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5878 136 Size: 107 122 71 631 605 4206 1525 Fragments arranged by size: 4206 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7262 7262 Size: 7267 BlpI GC'TnA_GC Cuts at: 3695 4684 3695 Size: 989 6278 Bme1580I G_kGCm'C Cuts at: 1790 2364 4106 5328 6484 1790 Size: 574 1742 1222 1156 2573 Fragments arranged by size: 2573 1742 1222 1156 574 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7267 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3644 4998 5956 Size: 416 53 83 399 24 2227 1354 958 Cuts at: 5956 442 Size: 1753 Fragments arranged by size: 2227 1753 1354 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7267 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2090 3024 3264 4104 5927 1239 Size: 851 934 240 840 1823 2579 Fragments arranged by size: 2579 1823 934 851 840 240 Bpu10I CC'TnA_GC Cuts at: 3129 3498 3129 Size: 369 6898 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4349 4361 5392 6257 6555 4349 Size: 12 1031 865 298 5061 Fragments arranged by size: 5061 1031 865 298 12 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2842 5930 882 Size: 617 1343 3088 2219 Fragments arranged by size: 3088 2219 1343 617 BsaAI yAC'GTr Cuts at: 493 1204 4564 5058 493 Size: 711 3360 494 2702 Fragments arranged by size: 3360 2702 711 494 BsaBI GATnn'nnATC Cuts at: 7261 7261 Size: 7267 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5074 5456 6091 275 Size: 53 83 186 936 3541 382 635 1451 Fragments arranged by size: 3541 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4028 4401 5700 6098 6372 6519 6930 4028 Size: 373 1299 398 274 147 411 4365 Fragments arranged by size: 4365 1299 411 398 373 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3495 3525 4090 4120 1178 Size: 30 165 30 2092 30 565 30 4325 Fragments arranged by size: 4325 2092 565 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4206 1186 Size: 3020 4247 BseYI C'CCAG_C Cuts at: 3699 6470 6966 3699 Size: 2771 496 4000 Fragments arranged by size: 4000 2771 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3626 3876 4214 1163 Size: 573 1890 250 338 4216 Fragments arranged by size: 4216 1890 573 338 250 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4717 5478 6506 1540 Size: 275 186 2716 761 1028 2301 Fragments arranged by size: 2716 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2364 3638 4320 4332 5328 5413 6484 2364 Size: 1274 682 12 996 85 1071 3147 Fragments arranged by size: 3147 1274 1071 996 682 85 12 BsmI GAATG_Cn' Cuts at: 2741 4778 4871 2741 Size: 2037 93 5137 Fragments arranged by size: 5137 2037 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2364 3638 4106 4320 4332 4586 5328 Size: 399 574 1274 468 214 12 254 742 Cuts at: 5328 5413 6484 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1274 1071 742 574 468 399 254 214 85 12 BspEI T'CCGG_A Cuts at: 6930 6930 Size: 7267 BspHI T'CATG_A Cuts at: 5156 6886 5156 Size: 1730 5537 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2987 4622 844 Size: 2143 1635 3489 Fragments arranged by size: 3489 2143 1635 BsrBI CCG'CTC Cuts at: 1394 1823 5154 1394 Size: 429 3331 3507 Fragments arranged by size: 3507 3331 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3229 5762 5936 65 Size: 1183 1981 2533 174 1396 Fragments arranged by size: 2533 1981 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5911 6098 1700 Size: 110 4101 187 2869 Fragments arranged by size: 4101 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7267 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7267 BssSI C'ACGA_G Cuts at: 5327 6339 5327 Size: 1012 6255 BstAPI GCAn_nnn'nTGC Cuts at: 794 2279 794 Size: 1485 5782 BstBI TT'CG_AA Cuts at: 2007 6071 2007 Size: 4064 3203 BstEII G'GTnAC_C Cuts at: 1184 2950 1184 Size: 1766 5501 BstXI CCAn_nnnn'nTGG Cuts at: 4427 4427 Size: 7267 BstYI r'GATC_y Cuts at: 1972 2207 2324 2653 4597 4964 5349 5366 6818 Size: 235 117 329 1944 367 385 17 1452 Cuts at: 6818 6904 6916 7262 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1944 1452 385 367 346 329 235 117 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6715 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6528 Fragments arranged by size: 6528 683 56 BtsI GCAGTG_nn' Cuts at: 2790 3211 3636 4783 5577 5597 2790 Size: 421 425 1147 794 20 4460 Fragments arranged by size: 4460 1147 794 425 421 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7267 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7232 DraI TTT'AAA Cuts at: 2425 2572 4571 2425 Size: 147 1999 5121 Fragments arranged by size: 5121 1999 147 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6274 809 Size: 507 4958 1802 Fragments arranged by size: 4958 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3747 4126 4714 Size: 54 1425 50 275 18 1917 379 588 Cuts at: 4714 5603 7232 8 Size: 889 1629 43 Fragments arranged by size: 1917 1629 1425 889 588 379 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4714 1537 Size: 275 2902 4090 Fragments arranged by size: 4090 2902 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3301 4339 4478 5197 1353 Size: 1948 1038 139 719 3423 Fragments arranged by size: 3423 1948 1038 719 139 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5854 6228 6374 387 Size: 1064 138 4265 374 146 1280 Fragments arranged by size: 4265 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4330 4330 Size: 7267 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2090 3024 3264 4104 4287 4539 5330 Size: 393 458 934 240 840 183 252 791 Cuts at: 5330 5927 6714 6982 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 934 840 791 787 597 458 393 268 252 240 183 EcoO109I rG'GnC_Cy Cuts at: 2931 2931 Size: 7267 EcoRI G'AATT_C Cuts at: 2336 4576 4954 2336 Size: 2240 378 4649 Fragments arranged by size: 4649 2240 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2674 2674 Size: 7267 FspI TGC'GCA Cuts at: 5773 5773 Size: 7267 HaeII r_GCGC'y Cuts at: 1678 6414 1678 Size: 4736 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2522 2554 2915 2947 3593 Size: 32 135 32 950 32 361 32 646 Cuts at: 3593 3625 3704 3736 4090 4122 4345 4377 5908 Size: 32 79 32 354 32 223 32 1531 Cuts at: 5908 5940 5982 6014 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1531 950 646 361 354 223 135 79 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4611 4857 669 Size: 988 281 2673 246 3079 Fragments arranged by size: 3079 2673 988 281 246 HindIII A'AGCT_T Cuts at: 748 3170 748 Size: 2422 4845 HpaI GTT'AAC Cuts at: 4857 4857 Size: 7267 KpnI G_GTAC'C Cuts at: 3708 4592 3708 Size: 884 6383 MfeI C'AATT_G Cuts at: 4866 4866 Size: 7267 MluI A'CGCG_T Cuts at: 7143 7143 Size: 7267 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2055 4531 6381 6565 1305 Size: 750 2476 1850 184 2007 Fragments arranged by size: 2476 2007 1850 750 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3749 4128 10 Size: 54 1425 343 1917 379 3149 Fragments arranged by size: 3149 1917 1425 379 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3050 3854 3882 5225 5584 5743 518 Size: 648 1884 804 28 1343 359 159 2042 Fragments arranged by size: 2042 1884 1343 804 648 359 159 28 MspA1I CmG'CkG Cuts at: 1606 3536 4683 5360 6508 6753 1606 Size: 1930 1147 677 1148 245 2120 Fragments arranged by size: 2120 1930 1148 1147 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6715 NdeI CA'TA_TG Cuts at: 387 3855 4111 387 Size: 3468 256 3543 Fragments arranged by size: 3543 3468 256 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7267 NotI GC'GGCC_GC Cuts at: 4714 4714 Size: 7267 NspI r_CATG'y Cuts at: 1132 1229 3379 4625 6170 1132 Size: 97 2150 1246 1545 2229 Fragments arranged by size: 2229 2150 1545 1246 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7267 PciI A'CATG_T Cuts at: 3375 6166 3375 Size: 2791 4476 PflMI CCAn_nnn'nTGG Cuts at: 1108 2862 3790 1108 Size: 1754 928 4585 Fragments arranged by size: 4585 1754 928 PfoI T'CCnGG_A Cuts at: 4419 4419 Size: 7267 PmeI GTTT'AAAC Cuts at: 4571 4571 Size: 7267 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3495 3527 5336 5368 6849 6881 3495 Size: 32 1809 32 1481 32 3881 Fragments arranged by size: 3881 1809 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2931 2931 Size: 7267 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7267 PsiI TTA'TAA Cuts at: 4837 4837 Size: 7267 PstI C_TGCA'G Cuts at: 830 2797 4619 830 Size: 1967 1822 3478 Fragments arranged by size: 3478 1967 1822 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7267 PvuII CAG'CTG Cuts at: 3536 3536 Size: 7267 SacI G_AGCT'C Cuts at: 4332 4332 Size: 7267 SalI G'TCGA_C Cuts at: 1936 4609 1936 Size: 2673 4594 SbfI CC_TGCA'GG Cuts at: 4619 4619 Size: 7267 ScaI AGT'ACT Cuts at: 2225 5515 7227 2225 Size: 3290 1712 2265 Fragments arranged by size: 3290 2265 1712 SfcI C'TryA_G Cuts at: 826 1045 2793 3731 4615 5750 6431 6622 826 Size: 219 1748 938 884 1135 681 191 1471 Fragments arranged by size: 1748 1471 1135 938 884 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7267 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7267 SmaI CCC'GGG Cuts at: 1872 4594 1872 Size: 2722 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4364 4376 5371 6272 6534 820 Size: 197 926 2421 12 995 901 262 1553 Fragments arranged by size: 2421 1553 995 926 901 262 197 12 SnaBI TAC'GTA Cuts at: 493 4564 5058 493 Size: 4071 494 2702 Fragments arranged by size: 4071 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7267 SphI G_CATG'C Cuts at: 1132 4625 1132 Size: 3493 3774 SspI AAT'ATT Cuts at: 5 52 2576 2878 5191 5 Size: 47 2524 302 2313 2081 Fragments arranged by size: 2524 2313 2081 302 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7267 StyI C'CwwG_G Cuts at: 513 1065 3466 3708 4394 4461 4706 513 Size: 552 2401 242 686 67 245 3074 Fragments arranged by size: 3074 2401 686 552 245 242 67 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3679 3797 3866 5294 5311 5464 5649 Size: 1255 2099 118 69 1428 17 153 185 Cuts at: 5649 6094 325 Size: 445 1498 Fragments arranged by size: 2099 1498 1428 1255 445 185 153 118 69 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2223 2666 4176 Size: 274 80 33 51 1443 246 443 1510 Cuts at: 4176 5513 7225 96 Size: 1337 1712 138 Fragments arranged by size: 1712 1510 1443 1337 443 274 246 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3393 3425 3941 3973 7227 7259 3393 Size: 32 516 32 3254 32 3401 Fragments arranged by size: 3401 3254 516 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3587 3644 4073 1316 Size: 2271 57 429 4510 Fragments arranged by size: 4510 2271 429 57 XbaI T'CTAG_A Cuts at: 4603 4603 Size: 7267 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3860 3860 Size: 7267 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7267 XmaI C'CCGG_G Cuts at: 1870 4592 1870 Size: 2722 4545 XmnI GAAnn'nnTTC Cuts at: 3377 5396 3377 Size: 2019 5248 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5075 276 Size: 53 83 186 936 3541 2468 Fragments arranged by size: 3541 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI