Restriction map for
pFN21ASDA1259
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# NO CUT ENZYMES ( 93 enzymes) #
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AatII Acc65I AccII AclI AcyI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI AscI Asp718I AspEI AviII
AvrII BbeI BbrPI BcgI BclI
BfrI BlnI BsaAI BsaHI BsiEI
BsiWI Bsp1407I BspLU11I BsrBI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstZ17I CpoI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI Hin1I KasI KpnI KspI
MluI MvnI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflMI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Van91I XmaCI XmaI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AccI 1728 GTCTAC GT^[AC][GT]AC
AccIII 4628 TCCGGA T^CCGGA
AflIII 4442 ACGTGT A^C[GA][CT]GT
AspHI 1066 GAGCAC G[AT]GC[AT]^C
AvaI 4181 CTCGAG C^[CT]CG[GA]G
BamHI 198 GGATCC G^GATCC
BciVI 1226 GTATCC GTATCC(6/5)
BlpI 843 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 843 GCTCAGC GC^T[ACGT]AGC
BseAI 4628 TCCGGA T^CCGGA
BsiHKAI 1066 GAGCAC G[AT]GC[AT]^C
BsmBI 4218 CGTCTC CGTCTC(1/5)
BsoBI 4181 CTCGAG C^[CT]CG[GA]G
BspDI 1279 ATCGAT AT^CGAT
BspEI 4628 TCCGGA T^CCGGA
BsrFI 3665 ACCGGC [GA]^CCGG[CT]
BstAPI 4360 GCAGGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 843 GCTCAGC GC^T[ACGT]AGC
Cfr10I 3665 ACCGGC [GA]^CCGG[CT]
ClaI 1279 ATCGAT AT^CGAT
DraI 1339 TTTAAA TTT^AAA
HincII 1303 GTTAAC GT[CT]^[GA]AC
HindII 1303 GTTAAC GT[CT]^[GA]AC
HpaI 1303 GTTAAC GTT^AAC
MfeI 1740 CAATTG C^AATTG
MroI 4628 TCCGGA T^CCGGA
MunI 1740 CAATTG C^AATTG
NdeI 578 CATATG CA^TATG
PaeR7I 4181 CTCGAG C^TCGAG
SspI 133 AATATT AAT^ATT
StuI 564 AGGCCT AGG^CCT
XbaI 3804 TCTAGA T^CTAGA
XcmI 1886 CCAGCTATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 4181 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AocI 1242 CCTAAGG CC^T[ACGT]AGG
2829 CCTGAGG
AseI 1609 ATTAAT AT^TAAT
2644 ATTAAT
AsnI 1609 ATTAAT AT^TAAT
2644 ATTAAT
Asp700I 463 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3694 GAACGCATTC
AspI 1395 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4268 GACGTGGTC
BbvCI 4166 CCTCAGC CCTCAGC(-5/-2)
2229 GCTGAGG
BglII 3455 AGATCT A^GATCT
4076 AGATCT
BsaBI 2182 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4077 GATCTCCATC
BsaI 2483 GGTCTC GGTCTC(1/5)
2621 GGTCTC
BsaWI 86 TCCGGT [AT]^CCGG[AT]
4628 TCCGGA
BspMI 1722 ACCTGC ACCTGC(4/8)
3651 GCAGGT
BstXI 3782 CCAAAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4109 CCATTCCCCTGG
Bsu36I 1242 CCTAAGG CC^T[ACGT]AGG
2829 CCTGAGG
Eco81I 1242 CCTAAGG CC^T[ACGT]AGG
2829 CCTGAGG
MamI 2182 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4077 GATCTCCATC
MslI 3504 CATCTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4038 CATTAGCGTG
NcoI 2060 CCATGG C^CATGG
3617 CCATGG
NspI 476 ACATGC [GA]CATG^[CT]
4136 ACATGC
PflFI 1395 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4268 GACGTGGTC
PshAI 107 GACAAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
741 GACCAAAGTC
PshBI 1609 ATTAAT AT^TAAT
2644 ATTAAT
ScaI 68 AGTACT AGT^ACT
551 AGTACT
SexAI 2954 ACCAGGT A^CC[AT]GGT
3971 ACCTGGT
Tsp45I 4133 GTGAC ^GT[GC]AC
4274 GTCAC
Tth111I 1395 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4268 GACGTGGTC
XmnI 463 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3694 GAACGCATTC
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# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApaI ApoI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspHI
BsrDI BsrI BssKI BssSI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DrdI DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I XhoII