Restriction map for
pFN21ASDA1259
(Linear) (Six-base) MAPSORT of: KIAA1259.seq Check: 7004 from: 1 to: 4668
KIAA1259.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1731 4668
Size: 1731 2937
AccI GT'mk_AC
Cuts at: 0 1729 4668
Size: 1729 2939
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 376 731 875 1375 2701 3133 4668
Size: 49 327 355 144 500 1326 432 1535
Fragments arranged by size:
1535 1326 500 432 355 327 144 49
AflIII A'CryG_T
Cuts at: 0 4442 4668
Size: 4442 226
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 836 4111 4153 4489 4668
Size: 836 3275 42 336 179
Fragments arranged by size:
3275 836 336 179 42
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2329 2361 4553 4585 4668
Size: 2329 32 2192 32 83
Fragments arranged by size:
2329 2192 83 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1353 2316 2927 4668
Size: 1353 963 611 1741
Fragments arranged by size:
1741 1353 963 611
ApaI G_GGCC'C
Cuts at: 0 84 3162 3630 4668
Size: 84 3078 468 1038
Fragments arranged by size:
3078 1038 468 84
ApoI r'AATT_y
Cuts at: 0 231 333 631 1774 2122 2342 4238 4668
Size: 231 102 298 1143 348 220 1896 430
Fragments arranged by size:
1896 1143 430 348 298 231 220 102
AseI AT'TA_AT
Cuts at: 0 1610 2645 4668
Size: 1610 1035 2023
Fragments arranged by size:
2023 1610 1035
AvaI C'yCGr_G
Cuts at: 0 4181 4668
Size: 4181 487
BamHI G'GATC_C
Cuts at: 0 198 4668
Size: 198 4470
BanI G'GyrC_C
Cuts at: 0 16 605 1745 3682 4645 4668
Size: 16 589 1140 1937 963 23
Fragments arranged by size:
1937 1140 963 589 23 16
BanII G_rGCy'C
Cuts at: 0 84 2079 2380 3162 3630 4668
Size: 84 1995 301 782 468 1038
Fragments arranged by size:
1995 1038 782 468 301 84
BbsI GAAGACnn'nnnn_
Cuts at: 0 434 1262 1849 2360 2547 3790 4668
Size: 434 828 587 511 187 1243 878
Fragments arranged by size:
1243 878 828 587 511 434 187
BbvCI CC'TCA_GC
Cuts at: 0 2230 4167 4668
Size: 2230 1937 501
Fragments arranged by size:
2230 1937 501
BciVI GTATCCnnnnn_n'
Cuts at: 0 1237 4668
Size: 1237 3431
BglI GCCn_nnn'nGGC
Cuts at: 0 970 1444 1669 1678 4668
Size: 970 474 225 9 2990
Fragments arranged by size:
2990 970 474 225 9
BglII A'GATC_T
Cuts at: 0 3455 4076 4668
Size: 3455 621 592
Fragments arranged by size:
3455 621 592
BlpI GC'TnA_GC
Cuts at: 0 844 4668
Size: 844 3824
Bme1580I G_kGCm'C
Cuts at: 0 84 1008 3162 3630 4668
Size: 84 924 2154 468 1038
Fragments arranged by size:
2154 1038 924 468 84
BmgBI CAC'GTC
Cuts at: 0 4270 4668
Size: 4270 398
BmrI ACTGGGnnnn_n'
Cuts at: 0 183 1475 3550 4668
Size: 183 1292 2075 1118
Fragments arranged by size:
2075 1292 1118 183
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 275 1854 2675 3239 3418 3554 4668
Size: 275 1579 821 564 179 136 1114
Fragments arranged by size:
1579 1114 821 564 275 179 136
Bpu10I CC'TnA_GC
Cuts at: 0 1961 2230 3636 4167 4668
Size: 1961 269 1406 531 501
Fragments arranged by size:
1961 1406 531 501 269
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1954 3322 3329 4668
Size: 1954 1368 7 1339
Fragments arranged by size:
1954 1368 1339 7
BsaI GGTCTCn'nnnn_
Cuts at: 0 2489 2627 4668
Size: 2489 138 2041
Fragments arranged by size:
2489 2041 138
BsaBI GATnn'nnATC
Cuts at: 0 2186 4081 4668
Size: 2186 1895 587
Fragments arranged by size:
2186 1895 587
BsaWI w'CCGG_w
Cuts at: 0 86 4628 4668
Size: 86 4542 40
Fragments arranged by size:
4542 86 40
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1283 1286 1313 1316 2469 2499 4090 4120
Size: 1283 3 27 3 1153 30 1591 30
Cuts at: 4120 4147 4177 4179 4209 4668
Size: 27 30 2 30 459
Fragments arranged by size:
1591 1283 1153 459 30 30 30 30
27 27 3 3 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1307 2247 2976 4155 4668
Size: 1307 940 729 1179 513
Fragments arranged by size:
1307 1179 940 729 513
BseYI C'CCAG_C
Cuts at: 0 4158 4668
Size: 4158 510
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 21 581 1924 3110 4081 4668
Size: 21 560 1343 1186 971 587
Fragments arranged by size:
1343 1186 971 587 560 21
BsiHKAI G_wGCw'C
Cuts at: 0 1070 4668
Size: 1070 3598
BsmI GAATG_Cn'
Cuts at: 0 383 825 1365 1616 3698 4668
Size: 383 442 540 251 2082 970
Fragments arranged by size:
2082 970 540 442 383 251
BsmBI CGTCTCn'nnnn_
Cuts at: 0 4224 4668
Size: 4224 444
Bsp1286I G_dGCh'C
Cuts at: 0 84 1008 1070 2079 2380 3162 3630 4668
Size: 84 924 62 1009 301 782 468 1038
Fragments arranged by size:
1038 1009 924 782 468 301 84 62
BspEI T'CCGG_A
Cuts at: 0 4628 4668
Size: 4628 40
BspHI T'CATG_A
Cuts at: 0 1193 1218 1806 2414 4668
Size: 1193 25 588 608 2254
Fragments arranged by size:
2254 1193 608 588 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1731 3642 4668
Size: 1731 1911 1026
Fragments arranged by size:
1911 1731 1026
BsrDI GCAATG_nn'
Cuts at: 0 235 1658 3084 4382 4668
Size: 235 1423 1426 1298 286
Fragments arranged by size:
1426 1423 1298 286 235
BsrFI r'CCGG_y
Cuts at: 0 3665 4668
Size: 3665 1003
BssSI C'ACGA_G
Cuts at: 0 1001 2567 3044 3527 4668
Size: 1001 1566 477 483 1141
Fragments arranged by size:
1566 1141 1001 483 477
BstAPI GCAn_nnn'nTGC
Cuts at: 0 4366 4668
Size: 4366 302
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3789 4116 4668
Size: 3789 327 552
Fragments arranged by size:
3789 552 327
BstYI r'GATC_y
Cuts at: 0 198 1997 3455 4076 4668
Size: 198 1799 1458 621 592
Fragments arranged by size:
1799 1458 621 592 198
Bsu36I CC'TnA_GG
Cuts at: 0 1243 2830 4668
Size: 1243 1587 1838
Fragments arranged by size:
1838 1587 1243
BtgI C'CryG_G
Cuts at: 0 2060 3617 4355 4668
Size: 2060 1557 738 313
Fragments arranged by size:
2060 1557 738 313
BtsI GCAGTG_nn'
Cuts at: 0 2942 3062 3148 3554 4095 4137 4371 4668
Size: 2942 120 86 406 541 42 234 297
Fragments arranged by size:
2942 541 406 297 234 120 86 42
ClaI AT'CG_AT
Cuts at: 0 1280 4668
Size: 1280 3388
DraI TTT'AAA
Cuts at: 0 1341 4668
Size: 1341 3327
DrdI GACnn_nn'nnGTC
Cuts at: 0 2959 4141 4624 4668
Size: 2959 1182 483 44
Fragments arranged by size:
2959 1182 483 44
EaeI y'GGCC_r
Cuts at: 0 1586 2729 3035 4668
Size: 1586 1143 306 1633
Fragments arranged by size:
1633 1586 1143 306
EarI CTCTTCn'nnn_
Cuts at: 0 684 3715 3809 3842 4536 4668
Size: 684 3031 94 33 694 132
Fragments arranged by size:
3031 694 684 132 94 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 4449 4668
Size: 4449 219
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 275 376 731 875 1375 1854 2675
Size: 49 226 101 355 144 500 479 821
Cuts at: 2675 2701 3133 3239 3418 3554 4668
Size: 26 432 106 179 136 1114
Fragments arranged by size:
1114 821 500 479 432 355 226 179
144 136 106 101 49 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1018 2769 3397 3432 4668
Size: 1018 1751 628 35 1236
Fragments arranged by size:
1751 1236 1018 628 35
EcoO109I rG'GnC_Cy
Cuts at: 0 80 81 1350 1702 2042 3158 3159 3357
Size: 80 1 1269 352 340 1116 1 198
Cuts at: 3357 3626 4668
Size: 269 1042
Fragments arranged by size:
1269 1116 1042 352 340 269 198 80
1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1011 2019 4547 4668
Size: 1011 1008 2528 121
Fragments arranged by size:
2528 1011 1008 121
HaeII r_GCGC'y
Cuts at: 0 1969 2803 4529 4668
Size: 1969 834 1726 139
Fragments arranged by size:
1969 1726 834 139
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 3004 3036 3433 3465 3530 3562 4147 4179
Size: 3004 32 397 32 65 32 585 32
Cuts at: 4179 4668
Size: 489
Fragments arranged by size:
3004 585 489 397 65 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1305 4668
Size: 1305 3363
HindIII A'AGCT_T
Cuts at: 0 887 925 1091 4668
Size: 887 38 166 3577
Fragments arranged by size:
3577 887 166 38
HpaI GTT'AAC
Cuts at: 0 1305 4668
Size: 1305 3363
MfeI C'AATT_G
Cuts at: 0 1740 4668
Size: 1740 2928
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 1091 1972 1999 2640 3800 4031 4668
Size: 71 1020 881 27 641 1160 231 637
Fragments arranged by size:
1160 1020 881 641 637 231 71 27
MscI TGG'CCA
Cuts at: 0 1588 2731 3037 4668
Size: 1588 1143 306 1631
Fragments arranged by size:
1631 1588 1143 306
MslI CAynn'nnrTG
Cuts at: 0 3508 4042 4668
Size: 3508 534 626
Fragments arranged by size:
3508 626 534
MspA1I CmG'CkG
Cuts at: 0 816 1104 1435 1896 1926 2891 2970 3217
Size: 816 288 331 461 30 965 79 247
Cuts at: 3217 3735 4261 4405 4417 4668
Size: 518 526 144 12 251
Fragments arranged by size:
965 816 526 518 461 331 288 251
247 144 79 30 12
NcoI C'CATG_G
Cuts at: 0 2060 3617 4668
Size: 2060 1557 1051
Fragments arranged by size:
2060 1557 1051
NdeI CA'TA_TG
Cuts at: 0 579 4668
Size: 579 4089
NspI r_CATG'y
Cuts at: 0 480 4140 4668
Size: 480 3660 528
Fragments arranged by size:
3660 528 480
PfoI T'CCnGG_A
Cuts at: 0 1229 4668
Size: 1229 3439
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 437 469 4668
Size: 437 32 4199
Fragments arranged by size:
4199 437 32
PpuMI rG'GwC_Cy
Cuts at: 0 1702 2042 3357 4668
Size: 1702 340 1315 1311
Fragments arranged by size:
1702 1315 1311 340
PshAI GACnn'nnGTC
Cuts at: 0 111 745 4668
Size: 111 634 3923
Fragments arranged by size:
3923 634 111
PspOMI G'GGCC_C
Cuts at: 0 80 3158 3626 4668
Size: 80 3078 468 1042
Fragments arranged by size:
3078 1042 468 80
PstI C_TGCA'G
Cuts at: 0 3177 3564 4266 4404 4416 4478 4668
Size: 3177 387 702 138 12 62 190
Fragments arranged by size:
3177 702 387 190 138 62 12
PvuII CAG'CTG
Cuts at: 0 816 1435 1896 2891 3217 4261 4417 4668
Size: 816 619 461 995 326 1044 156 251
Fragments arranged by size:
1044 995 816 619 461 326 251 156
SanDI GG'GwC_CC
Cuts at: 0 2042 4668
Size: 2042 2626
ScaI AGT'ACT
Cuts at: 0 70 553 4668
Size: 70 483 4115
Fragments arranged by size:
4115 483 70
SexAI A'CCwGG_T
Cuts at: 0 2954 3971 4668
Size: 2954 1017 697
Fragments arranged by size:
2954 1017 697
SfcI C'TryA_G
Cuts at: 0 304 555 2769 3173 3560 4262 4400 4412
Size: 304 251 2214 404 387 702 138 12
Cuts at: 4412 4474 4668
Size: 62 194
Fragments arranged by size:
2214 702 404 387 304 251 194 138
62 12
SmlI C'TyrA_G
Cuts at: 0 1969 3337 3344 4181 4668
Size: 1969 1368 7 837 487
Fragments arranged by size:
1969 1368 837 487 7
SspI AAT'ATT
Cuts at: 0 135 4668
Size: 135 4533
StuI AGG'CCT
Cuts at: 0 566 4668
Size: 566 4102
StyI C'CwwG_G
Cuts at: 0 1019 1638 2060 2141 3617 3713 4668
Size: 1019 619 422 81 1476 96 955
Fragments arranged by size:
1476 1019 955 619 422 96 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 4 3098 4668
Size: 4 3094 1570
Fragments arranged by size:
3094 1570 4
TatI w'GTAC_w
Cuts at: 0 68 551 1034 1650 4668
Size: 68 483 483 616 3018
Fragments arranged by size:
3018 616 483 483 68
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3188 3220 4090 4122 4668
Size: 3188 32 870 32 546
Fragments arranged by size:
3188 870 546 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1398 4271 4668
Size: 1398 2873 397
Fragments arranged by size:
2873 1398 397
XbaI T'CTAG_A
Cuts at: 0 3804 4668
Size: 3804 864
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1893 4668
Size: 1893 2775
XhoI C'TCGA_G
Cuts at: 0 4181 4668
Size: 4181 487
XmnI GAAnn'nnTTC
Cuts at: 0 467 3698 4668
Size: 467 3231 970
Fragments arranged by size:
3231 970 467
Enzymes that do cut:
AarI AccI AcuI AflIII AlfI AloI AlwNI ApaI
ApoI AseI AvaI BamHI BanI BanII BbsI BbvCI
BciVI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrDI BsrFI BssSI BstAPI BstXI BstYI Bsu36I BtgI
BtsI ClaI DraI DrdI EaeI EarI EciI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I HincII HindIII HpaI
MfeI MmeI MscI MslI MspA1I NcoI NdeI NspI
PfoI PpiI PpuMI PshAI PspOMI PstI PvuII SanDI
ScaI SexAI SfcI SmlI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
ApaLI AscI AsiSI AvrII BaeI BbeI BcgI BclI
BfrBI BmtI BplI BsaAI BsaHI BsiEI BsiWI BsrBI
BsrGI BssHII BstBI BstEII BstZ17I BtgZI CspCI DraIII
EagI EcoICRI EcoRI EcoRV FseI FspI FspAI KasI
KpnI MluI NaeI NarI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PflMI PmeI PmlI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SapI
SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SwaI XmaI ZraI