(Circular) (Six-base) MAPSORT of: pFN21ASDA1258.seq Check: 2286 from: 1 to: 6091 pFN21ASDA1258 6091 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3349 3349 Size: 6091 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3901 278 Size: 53 83 186 936 2365 2468 Fragments arranged by size: 2468 2365 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3434 1937 Size: 1497 4594 Acc65I G'GTAC_C Cuts at: 3412 3412 Size: 6091 AclI AA'CG_TT Cuts at: 1563 4218 4591 1563 Size: 2655 373 3063 Fragments arranged by size: 3063 2655 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3112 4154 5538 5806 1632 Size: 1480 1042 1384 268 1917 Fragments arranged by size: 1917 1480 1384 1042 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5894 AflIII A'CryG_T Cuts at: 1203 4990 5967 1203 Size: 3787 977 1327 Fragments arranged by size: 3787 1327 977 AgeI A'CCGG_T Cuts at: 4922 4922 Size: 6091 AhdI GACnn_n'nnGTC Cuts at: 4820 4820 Size: 6091 AleI CACnn'nnGTG Cuts at: 1166 2810 1166 Size: 1644 4447 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2327 2878 1184 Size: 1143 551 4397 Fragments arranged by size: 4397 1143 551 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2346 2378 2346 Size: 32 6059 AlwNI CAG_nnn'CTG Cuts at: 3834 5406 3834 Size: 1572 4519 ApaLI G'TGCA_C Cuts at: 4148 5304 4148 Size: 1156 4935 ApoI r'AATT_y Cuts at: 1493 2498 3203 3400 3626 3778 5887 1493 Size: 1005 705 197 226 152 2109 1697 Fragments arranged by size: 2109 1697 1005 705 226 197 152 AseI AT'TA_AT Cuts at: 160 1032 3087 4645 160 Size: 872 2055 1558 1606 Fragments arranged by size: 2055 1606 1558 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6091 AvaI C'yCGr_G Cuts at: 1870 1943 3416 1870 Size: 73 1473 4545 Fragments arranged by size: 4545 1473 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2997 3030 2997 Size: 33 6058 BamHI G'GATC_C Cuts at: 3421 3788 3421 Size: 367 5724 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3412 4867 618 Size: 325 217 627 1625 1455 1842 Fragments arranged by size: 1842 1625 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3410 1391 Size: 2019 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3118 928 Size: 971 1219 3901 Fragments arranged by size: 3901 1219 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4282 4316 1903 Size: 34 2345 34 3678 Fragments arranged by size: 3678 2345 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3983 5193 3983 Size: 1210 4881 BclI T'GATC_A Cuts at: 1548 3219 1548 Size: 1671 4420 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4702 136 Size: 107 122 71 631 605 3030 1525 Fragments arranged by size: 3030 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6086 6086 Size: 6091 BlpI GC'TnA_GC Cuts at: 2945 3508 2945 Size: 563 5528 Bme1580I G_kGCm'C Cuts at: 1790 4152 5308 1790 Size: 2362 1156 2573 Fragments arranged by size: 2573 2362 1156 BmgBI CAC'GTC Cuts at: 1318 3013 1318 Size: 1695 4396 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2708 2784 3822 Size: 416 53 83 399 24 1291 76 1038 Cuts at: 3822 4780 442 Size: 958 1753 Fragments arranged by size: 1753 1291 1038 958 416 399 83 76 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6091 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4751 1239 Size: 3512 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4216 5081 5379 4216 Size: 865 298 4928 Fragments arranged by size: 4928 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2561 4754 882 Size: 617 1062 2193 2219 Fragments arranged by size: 2219 2193 1062 617 BsaAI yAC'GTr Cuts at: 493 1204 3388 3882 493 Size: 711 2184 494 2702 Fragments arranged by size: 2702 2184 711 494 BsaBI GATnn'nnATC Cuts at: 6085 6085 Size: 6091 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3898 4280 4915 275 Size: 53 83 186 936 2365 382 635 1451 Fragments arranged by size: 2365 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4524 4922 5196 5343 5754 4524 Size: 398 274 147 411 4861 Fragments arranged by size: 4861 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5866 Fragments arranged by size: 5866 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2111 2919 1186 Size: 925 808 4358 Fragments arranged by size: 4358 925 808 BseYI C'CCAG_C Cuts at: 3001 5294 5790 3001 Size: 2293 496 3302 Fragments arranged by size: 3302 2293 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5518 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3541 4302 5330 1540 Size: 275 186 1540 761 1028 2301 Fragments arranged by size: 2301 1540 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4152 4237 5308 4152 Size: 85 1071 4935 Fragments arranged by size: 4935 1071 85 BsmI GAATG_Cn' Cuts at: 3602 3695 3602 Size: 93 5998 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3410 4152 4237 5308 1391 Size: 399 1620 742 85 1071 2174 Fragments arranged by size: 2174 1620 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5754 5754 Size: 6091 BspHI T'CATG_A Cuts at: 3980 5710 3980 Size: 1730 4361 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2099 2319 3349 3446 844 Size: 1255 220 1030 97 3489 Fragments arranged by size: 3489 1255 1030 220 97 BsrBI CCG'CTC Cuts at: 1394 1823 2033 3978 1394 Size: 429 210 1945 3507 Fragments arranged by size: 3507 1945 429 210 BsrDI GCAATG_nn' Cuts at: 65 1248 2706 4586 4760 65 Size: 1183 1458 1880 174 1396 Fragments arranged by size: 1880 1458 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4735 4922 1700 Size: 110 2925 187 2869 Fragments arranged by size: 2925 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6091 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6091 BssSI C'ACGA_G Cuts at: 4151 5163 4151 Size: 1012 5079 BstAPI GCAn_nnn'nTGC Cuts at: 794 2529 794 Size: 1735 4356 BstBI TT'CG_AA Cuts at: 2007 4895 2007 Size: 2888 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6091 BstYI r'GATC_y Cuts at: 1972 3421 3788 4173 4190 5642 5728 5740 6086 Size: 1449 367 385 17 1452 86 12 346 Cuts at: 6086 1972 Size: 1977 Fragments arranged by size: 1977 1452 1449 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2097 513 Size: 552 1032 4507 Fragments arranged by size: 4507 1032 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2899 487 Size: 683 56 1673 3679 Fragments arranged by size: 3679 1673 683 56 BtsI GCAGTG_nn' Cuts at: 2956 3066 3607 4401 4421 2956 Size: 110 541 794 20 4626 Fragments arranged by size: 4626 794 541 110 20 ClaI AT'CG_AT Cuts at: 1554 2582 1554 Size: 1028 5063 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6056 DraI TTT'AAA Cuts at: 3395 3395 Size: 6091 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5098 809 Size: 507 3782 1802 Fragments arranged by size: 3782 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2184 3538 4427 Size: 54 1425 50 275 18 354 1354 889 Cuts at: 4427 6056 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1354 889 354 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3538 1537 Size: 275 1726 4090 Fragments arranged by size: 4090 1726 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3049 3330 4021 1353 Size: 1696 281 691 3423 Fragments arranged by size: 3423 1696 691 281 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3366 4678 5052 5198 387 Size: 1064 138 1777 1312 374 146 1280 Fragments arranged by size: 1777 1312 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3112 4154 4751 5538 5806 1239 Size: 393 1480 1042 597 787 268 1524 Fragments arranged by size: 1524 1480 1042 787 597 393 268 EcoRI G'AATT_C Cuts at: 3400 3778 3400 Size: 378 5713 FspI TGC'GCA Cuts at: 4597 4597 Size: 6091 HaeII r_GCGC'y Cuts at: 1678 5238 1678 Size: 3560 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2898 2930 4732 4764 4806 Size: 32 135 32 1326 32 1802 32 42 Cuts at: 4806 4838 1373 Size: 32 2626 Fragments arranged by size: 2626 1802 1326 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3435 3681 669 Size: 988 281 1497 246 3079 Fragments arranged by size: 3079 1497 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6091 HpaI GTT'AAC Cuts at: 3681 3681 Size: 6091 KpnI G_GTAC'C Cuts at: 3416 3416 Size: 6091 MfeI C'AATT_G Cuts at: 3690 3690 Size: 6091 MluI A'CGCG_T Cuts at: 5967 5967 Size: 6091 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3261 5205 5389 1305 Size: 1956 1944 184 2007 Fragments arranged by size: 2007 1956 1944 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2186 10 Size: 54 1425 343 354 3915 Fragments arranged by size: 3915 1425 354 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2102 2271 2745 2810 4049 4408 4567 Size: 648 936 169 474 65 1239 359 159 Cuts at: 4567 518 Size: 2042 Fragments arranged by size: 2042 1239 936 648 474 359 169 159 65 MspA1I CmG'CkG Cuts at: 1606 2139 2509 3507 4184 5332 5577 1606 Size: 533 370 998 677 1148 245 2120 Fragments arranged by size: 2120 1148 998 677 533 370 245 NcoI C'CATG_G Cuts at: 513 1065 2097 513 Size: 552 1032 4507 Fragments arranged by size: 4507 1032 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6091 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6091 NotI GC'GGCC_GC Cuts at: 3538 3538 Size: 6091 NspI r_CATG'y Cuts at: 1132 1229 3449 4994 1132 Size: 97 2220 1545 2229 Fragments arranged by size: 2229 2220 1545 97 PacI TTA_AT'TAA Cuts at: 3087 3087 Size: 6091 PasI CC'CwG_GG Cuts at: 1348 3160 1348 Size: 1812 4279 PciI A'CATG_T Cuts at: 4990 4990 Size: 6091 PflMI CCAn_nnn'nTGG Cuts at: 1108 2193 3160 1108 Size: 1085 967 4039 Fragments arranged by size: 4039 1085 967 PmeI GTTT'AAAC Cuts at: 3395 3395 Size: 6091 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4160 4192 5673 5705 4160 Size: 32 1481 32 4546 Fragments arranged by size: 4546 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2072 1613 Size: 459 5632 PsiI TTA'TAA Cuts at: 2796 3661 2796 Size: 865 5226 PstI C_TGCA'G Cuts at: 830 2353 2878 3443 830 Size: 1523 525 565 3478 Fragments arranged by size: 3478 1523 565 525 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6091 SalI G'TCGA_C Cuts at: 1936 3433 1936 Size: 1497 4594 SapI GCTCTTCn'nnn_ Cuts at: 3049 3049 Size: 6091 SbfI CC_TGCA'GG Cuts at: 3443 3443 Size: 6091 ScaI AGT'ACT Cuts at: 4339 6051 4339 Size: 1712 4379 SfcI C'TryA_G Cuts at: 826 1045 2349 2874 3439 4574 5255 5446 826 Size: 219 1304 525 565 1135 681 191 1471 Fragments arranged by size: 1471 1304 1135 681 565 525 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6091 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6091 SmaI CCC'GGG Cuts at: 1872 3418 1872 Size: 1546 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4195 5096 5358 820 Size: 197 926 2252 901 262 1553 Fragments arranged by size: 2252 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3388 3882 493 Size: 2895 494 2702 Fragments arranged by size: 2895 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6091 SphI G_CATG'C Cuts at: 1132 3449 1132 Size: 2317 3774 SspI AAT'ATT Cuts at: 5 52 3331 4015 5 Size: 47 3279 684 2081 Fragments arranged by size: 3279 2081 684 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6091 StyI C'CwwG_G Cuts at: 513 1065 2097 3530 513 Size: 552 1032 1433 3074 Fragments arranged by size: 3074 1433 1032 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2681 4118 4135 4288 4473 4918 325 Size: 1255 1101 1437 17 153 185 445 1498 Fragments arranged by size: 1498 1437 1255 1101 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2337 4337 6049 Size: 274 80 33 51 1443 360 2000 1712 Cuts at: 6049 96 Size: 138 Fragments arranged by size: 2000 1712 1443 360 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3345 3377 6051 6083 3345 Size: 32 2674 32 3353 Fragments arranged by size: 3353 2674 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6091 XbaI T'CTAG_A Cuts at: 2633 3427 2633 Size: 794 5297 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2322 2322 Size: 6091 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6091 XmaI C'CCGG_G Cuts at: 1870 3416 1870 Size: 1546 4545 XmnI GAAnn'nnTTC Cuts at: 2891 4220 2891 Size: 1329 4762 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3899 276 Size: 53 83 186 936 2365 2468 Fragments arranged by size: 2468 2365 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PacI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI