(Circular) (Six-base) MAPSORT of: pFN21ASDA1257.seq Check: 9828 from: 1 to: 5956 pFN21ASDA1257 5956 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3766 278 Size: 53 83 186 936 2230 2468 Fragments arranged by size: 2468 2230 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3299 1937 Size: 1362 4594 Acc65I G'GTAC_C Cuts at: 3191 3277 3191 Size: 86 5870 AclI AA'CG_TT Cuts at: 1563 4083 4456 1563 Size: 2520 373 3063 Fragments arranged by size: 3063 2520 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2153 4019 5403 5671 1632 Size: 521 1866 1384 268 1917 Fragments arranged by size: 1917 1866 1384 521 268 AfeI AGC'GCT Cuts at: 3151 3151 Size: 5956 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5759 AflIII A'CryG_T Cuts at: 1203 4855 5832 1203 Size: 3652 977 1327 Fragments arranged by size: 3652 1327 977 AgeI A'CCGG_T Cuts at: 4787 4787 Size: 5956 AhdI GACnn_n'nnGTC Cuts at: 2614 4685 2614 Size: 2071 3885 AleI CACnn'nnGTG Cuts at: 1166 2496 1166 Size: 1330 4626 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5956 AlwNI CAG_nnn'CTG Cuts at: 3223 3699 5271 3223 Size: 476 1572 3908 Fragments arranged by size: 3908 1572 476 ApaI G_GGCC'C Cuts at: 2152 2152 Size: 5956 ApaLI G'TGCA_C Cuts at: 4013 5169 4013 Size: 1156 4800 ApoI r'AATT_y Cuts at: 1493 2249 2569 3265 3491 3643 5752 1493 Size: 756 320 696 226 152 2109 1697 Fragments arranged by size: 2109 1697 756 696 320 226 152 AseI AT'TA_AT Cuts at: 160 1032 2984 4510 160 Size: 872 1952 1526 1606 Fragments arranged by size: 1952 1606 1526 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5956 AvaI C'yCGr_G Cuts at: 1870 1943 3281 1870 Size: 73 1338 4545 Fragments arranged by size: 4545 1338 73 BamHI G'GATC_C Cuts at: 3286 3653 3286 Size: 367 5589 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3191 3277 4732 618 Size: 325 217 627 1404 86 1455 1842 Fragments arranged by size: 1842 1455 1404 627 325 217 86 BanII G_rGCy'C Cuts at: 1391 2073 2152 2850 3275 1391 Size: 682 79 698 425 4072 Fragments arranged by size: 4072 698 682 425 79 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4985 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2167 2201 4147 4181 1903 Size: 34 230 34 1946 34 3678 Fragments arranged by size: 3678 1946 230 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3848 5058 3848 Size: 1210 4746 BclI T'GATC_A Cuts at: 1548 1548 Size: 5956 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3244 4567 136 Size: 107 122 71 631 605 1572 1323 1525 Fragments arranged by size: 1572 1525 1323 631 605 122 107 71 BglII A'GATC_T Cuts at: 5951 5951 Size: 5956 BlpI GC'TnA_GC Cuts at: 3373 3373 Size: 5956 Bme1580I G_kGCm'C Cuts at: 1790 2152 2232 4017 5173 1790 Size: 362 80 1785 1156 2573 Fragments arranged by size: 2573 1785 1156 362 80 BmgBI CAC'GTC Cuts at: 1318 2119 2227 2668 1318 Size: 801 108 441 4606 Fragments arranged by size: 4606 801 441 108 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3687 4645 442 Size: 416 53 83 399 24 2270 958 1753 Fragments arranged by size: 2270 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5956 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2092 2179 2092 Size: 87 5869 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2480 4616 1239 Size: 1241 2136 2579 Fragments arranged by size: 2579 2136 1241 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2608 4081 4946 5244 2608 Size: 1473 865 298 3320 Fragments arranged by size: 3320 1473 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3042 4619 882 Size: 617 1543 1577 2219 Fragments arranged by size: 2219 1577 1543 617 BsaAI yAC'GTr Cuts at: 493 1204 2236 3253 3747 493 Size: 711 1032 1017 494 2702 Fragments arranged by size: 2702 1032 1017 711 494 BsaBI GATnn'nnATC Cuts at: 2972 5950 2972 Size: 2978 2978 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3763 4145 4780 275 Size: 53 83 186 936 2230 382 635 1451 Fragments arranged by size: 2230 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4389 4787 5061 5208 5619 4389 Size: 398 274 147 411 4726 Fragments arranged by size: 4726 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5731 Fragments arranged by size: 5731 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2068 3097 1186 Size: 882 1029 4045 Fragments arranged by size: 4045 1029 882 BseYI C'CCAG_C Cuts at: 3213 5159 5655 3213 Size: 1946 496 3514 Fragments arranged by size: 3514 1946 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2197 1163 Size: 573 461 4922 Fragments arranged by size: 4922 573 461 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3406 4167 5195 1540 Size: 275 186 1405 761 1028 2301 Fragments arranged by size: 2301 1405 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4017 4102 5173 4017 Size: 85 1071 4800 Fragments arranged by size: 4800 1071 85 BsmI GAATG_Cn' Cuts at: 3467 3560 3467 Size: 93 5863 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2073 2152 2232 2850 3275 4017 4102 Size: 399 283 79 80 618 425 742 85 Cuts at: 4102 5173 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 618 425 399 283 85 80 79 BspEI T'CCGG_A Cuts at: 5619 5619 Size: 5956 BspHI T'CATG_A Cuts at: 3845 5575 3845 Size: 1730 4226 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3311 844 Size: 2467 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3181 3843 1394 Size: 429 1358 662 3507 Fragments arranged by size: 3507 1358 662 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4451 4625 65 Size: 1183 3203 174 1396 Fragments arranged by size: 3203 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2651 3188 4600 4787 1700 Size: 110 841 537 1412 187 2869 Fragments arranged by size: 2869 1412 841 537 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5956 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5956 BssSI C'ACGA_G Cuts at: 2104 4016 5028 2104 Size: 1912 1012 3032 Fragments arranged by size: 3032 1912 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3223 794 Size: 2429 3527 BstBI TT'CG_AA Cuts at: 2007 4760 2007 Size: 2753 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5956 BstXI CCAn_nnnn'nTGG Cuts at: 2263 2950 3166 2263 Size: 687 216 5053 Fragments arranged by size: 5053 687 216 BstYI r'GATC_y Cuts at: 1972 3286 3653 4038 4055 5507 5593 5605 5951 Size: 1314 367 385 17 1452 86 12 346 Cuts at: 5951 1972 Size: 1977 Fragments arranged by size: 1977 1452 1314 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2486 513 Size: 552 1421 3983 Fragments arranged by size: 3983 1421 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5217 Fragments arranged by size: 5217 683 56 BtsI GCAGTG_nn' Cuts at: 3196 3472 4266 4286 3196 Size: 276 794 20 4866 Fragments arranged by size: 4866 794 276 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5956 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5921 DraI TTT'AAA Cuts at: 2709 3260 2709 Size: 551 5405 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4963 809 Size: 507 3647 1802 Fragments arranged by size: 3647 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2445 3168 3236 Size: 54 1425 50 275 18 615 723 68 Cuts at: 3236 3245 3403 4292 5921 8 Size: 9 158 889 1629 43 Fragments arranged by size: 1629 1425 889 723 615 275 158 68 54 50 43 18 9 EagI C'GGCC_G Cuts at: 1537 1812 3403 1537 Size: 275 1591 4090 Fragments arranged by size: 4090 1591 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2043 2757 3105 3886 1353 Size: 690 714 348 781 3423 Fragments arranged by size: 3423 781 714 690 348 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2772 4543 4917 5063 387 Size: 1064 138 1183 1771 374 146 1280 Fragments arranged by size: 1771 1280 1183 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2153 2480 4019 4616 5403 5671 1239 Size: 393 521 327 1539 597 787 268 1524 Fragments arranged by size: 1539 1524 787 597 521 393 327 268 EcoO109I rG'GnC_Cy Cuts at: 2053 2148 3134 2053 Size: 95 986 4875 Fragments arranged by size: 4875 986 95 EcoRI G'AATT_C Cuts at: 3265 3643 3265 Size: 378 5578 FspI TGC'GCA Cuts at: 4462 4462 Size: 5956 HaeII r_GCGC'y Cuts at: 1678 3153 5103 1678 Size: 1475 1950 2531 Fragments arranged by size: 2531 1950 1475 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2180 2212 2600 2632 2981 Size: 32 135 32 608 32 388 32 349 Cuts at: 2981 3013 4597 4629 4671 4703 1373 Size: 32 1584 32 42 32 2626 Fragments arranged by size: 2626 1584 608 388 349 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2422 3300 3546 669 Size: 988 281 484 878 246 3079 Fragments arranged by size: 3079 988 878 484 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5956 HpaI GTT'AAC Cuts at: 3546 3546 Size: 5956 KpnI G_GTAC'C Cuts at: 3195 3281 3195 Size: 86 5870 MfeI C'AATT_G Cuts at: 3555 3555 Size: 5956 MluI A'CGCG_T Cuts at: 5832 5832 Size: 5956 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2247 5070 5254 1305 Size: 942 2823 184 2007 Fragments arranged by size: 2823 2007 942 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2447 3238 10 Size: 54 1425 343 615 791 2728 Fragments arranged by size: 2728 1425 791 615 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2496 3914 4273 4432 518 Size: 648 1330 1418 359 159 2042 Fragments arranged by size: 2042 1418 1330 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3166 3372 4049 5197 5442 1606 Size: 1560 206 677 1148 245 2120 Fragments arranged by size: 2120 1560 1148 677 245 206 NaeI GCC'GGC Cuts at: 2653 2653 Size: 5956 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5404 NdeI CA'TA_TG Cuts at: 387 387 Size: 5956 NgoMIV G'CCGG_C Cuts at: 2651 2651 Size: 5956 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5956 NotI GC'GGCC_GC Cuts at: 3403 3403 Size: 5956 NspI r_CATG'y Cuts at: 1132 1229 2036 3314 4859 1132 Size: 97 807 1278 1545 2229 Fragments arranged by size: 2229 1545 1278 807 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5956 PciI A'CATG_T Cuts at: 4855 4855 Size: 5956 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5956 PfoI T'CCnGG_A Cuts at: 2457 2457 Size: 5956 PmeI GTTT'AAAC Cuts at: 3260 3260 Size: 5956 PmlI CAC'GTG Cuts at: 2236 2236 Size: 5956 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4025 4057 5538 5570 4025 Size: 32 1481 32 4411 Fragments arranged by size: 4411 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2053 2053 Size: 5956 PshAI GACnn'nnGTC Cuts at: 1613 3034 1613 Size: 1421 4535 PsiI TTA'TAA Cuts at: 3526 3526 Size: 5956 PspOMI G'GGCC_C Cuts at: 2148 2148 Size: 5956 PstI C_TGCA'G Cuts at: 830 3308 830 Size: 2478 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5956 PvuII CAG'CTG Cuts at: 3166 3166 Size: 5956 SalI G'TCGA_C Cuts at: 1936 3298 1936 Size: 1362 4594 SbfI CC_TGCA'GG Cuts at: 3308 3308 Size: 5956 ScaI AGT'ACT Cuts at: 4204 5916 4204 Size: 1712 4244 SfcI C'TryA_G Cuts at: 826 1045 3304 4439 5120 5311 826 Size: 219 2259 1135 681 191 1471 Fragments arranged by size: 2259 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3244 3244 Size: 5956 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5956 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5956 SmaI CCC'GGG Cuts at: 1872 3283 1872 Size: 1411 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2587 4060 4961 5223 820 Size: 197 926 644 1473 901 262 1553 Fragments arranged by size: 1553 1473 926 901 644 262 197 SnaBI TAC'GTA Cuts at: 493 3253 3747 493 Size: 2760 494 2702 Fragments arranged by size: 2760 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5956 SphI G_CATG'C Cuts at: 1132 3314 1132 Size: 2182 3774 SspI AAT'ATT Cuts at: 5 52 3880 5 Size: 47 3828 2081 Fragments arranged by size: 3828 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5956 StyI C'CwwG_G Cuts at: 513 1065 2139 3395 513 Size: 552 1074 1256 3074 Fragments arranged by size: 3074 1256 1074 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2500 2829 3983 4000 4153 4338 4783 Size: 1255 920 329 1154 17 153 185 445 Cuts at: 4783 325 Size: 1498 Fragments arranged by size: 1498 1255 1154 920 445 329 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4202 5914 96 Size: 274 80 33 51 1443 2225 1712 138 Fragments arranged by size: 2225 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2253 2285 5916 5948 2253 Size: 32 3631 32 2261 Fragments arranged by size: 3631 2261 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5956 XbaI T'CTAG_A Cuts at: 3292 3292 Size: 5956 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2455 2455 Size: 5956 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5956 XmaI C'CCGG_G Cuts at: 1870 3281 1870 Size: 1411 4545 XmnI GAAnn'nnTTC Cuts at: 4085 4085 Size: 5956 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3764 276 Size: 53 83 186 936 2230 2468 Fragments arranged by size: 2468 2230 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfoI SrfI SwaI