Restriction map for
pFN21ASDA1253
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# NO CUT ENZYMES ( 149 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI AlwNI AocI Aor51HI ApaI
AscI Asp700I Asp718I AspEI AvaII
AviII AvrII BalI BamHI BanII
BbeI BbrPI BbvCI BcgI BcnI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmAI BsmBI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
Cfr13I ClaI CpoI DraII DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FauI
FseI FspI HaeII HaeIII HapII
HhaI Hin1I HinP1I HindIII HpaI
HpaII KasI KpnI KspI MaeII
MluI MluNI MroI MscI MspI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PinAI PmaCI PmeI
PmlI PpuMI Psp1406I PvuI RsrII
SacI SacII SalI SapI Sau96I
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI Sse8387I
SspBI SspI StuI SwaI XbaI
XcmI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AcsI 385 AAATTT [GA]^AATT[CT]
Alw44I 361 GTGCAC G^TGCAC
ApaLI 361 GTGCAC G^TGCAC
ApoI 385 AAATTT [GA]^AATT[CT]
AseI 1056 ATTAAT AT^TAAT
AsnI 1056 ATTAAT AT^TAAT
AspI 822 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 570 CTCGAG C^[CT]CG[GA]G
BbsI 789 GTCTTC GAAGAC(2/6)
BciVI 38 GGATAC GTATCC(6/5)
BclI 323 TGATCA T^GATCA
BglII 772 AGATCT A^GATCT
BpmI 1093 CTGGAG CTGGAG(16/14)
BpuAI 789 GTCTTC GAAGAC(2/6)
BsaBI 1293 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 712 CTCCTC GAGGAG(10/8)
BsmI 418 GCATTC GAATGC(1/-1)
BsoBI 570 CTCGAG C^[CT]CG[GA]G
BspHI 1199 TCATGA T^CATGA
BspMI 472 GCAGGT ACCTGC(4/8)
BstAPI 394 GCAGCAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 772 AGATCT [GA]^GATC[CT]
DdeI 874 CTAAG C^T[ACGT]AG
DraI 382 TTTAAA TTT^AAA
DsaI 26 CCATGG C^C[GA][CT]GG
EcoT14I 26 CCATGG C^C[AT][AT]GG
FbaI 323 TGATCA T^GATCA
HgaI 8 GCGTC GACGC(5/10)
HincII 703 GTCAAC GT[CT]^[GA]AC
HindII 703 GTCAAC GT[CT]^[GA]AC
MamI 1293 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 398 CAATTG C^AATTG
MflI 772 AGATCT [GA]^GATC[CT]
MspA1I 1262 CAGCTG C[AC]G^C[GT]G
MunI 398 CAATTG C^AATTG
NcoI 26 CCATGG C^CATGG
PaeR7I 570 CTCGAG C^TCGAG
PflFI 822 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 769 CCAAGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1032 GACTC GAGTC(4/5)
PshAI 1260 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1056 ATTAAT AT^TAAT
PvuII 1262 CAGCTG CAG^CTG
RcaI 1199 TCATGA T^CATGA
SpeI 501 ACTAGT A^CTAGT
SphI 939 GCATGC GCATG^C
StyI 26 CCATGG C^C[AT][AT]GG
TaqI 571 TCGA T^CGA
Tsp45I 1144 GTCAC ^GT[GC]AC
Tth111I 822 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 571 TCGA T^CGA
Van91I 769 CCAAGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 570 CTCGAG C^TCGAG
XhoII 772 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
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AfaI 1001 GTAC GT^AC
1221 GTAC
AlwI 346 GATCC GGATC(4/5)
1087 GGATC
AspHI 21 GTGCTC G[AT]GC[AT]^C
361 GTGCAC
BanI 475 GGTGCC G^G[CT][GA]CC
1326 GGCACC
BsaJI 12 CCTGGG C^C[ACGT][ACGT]GG
26 CCATGG
BsiHKAI 21 GTGCTC G[AT]GC[AT]^C
361 GTGCAC
BsiYI 769 CCAAGATCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
910 CCAAAGGAAGG
BslI 769 CCAAGATCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
910 CCAAAGGAAGG
BssSI 1238 CTCGTG CACGAG(-5/-1)
1321 CTCGTG
Cac8I 31 GCGAGC GC[ACGT]^[ACGT]GC
939 GCATGC
EarI 1281 CTCTTC CTCTTC(1/4)
333 GAAGAG
HphI 1272 GGTGA GGTGA(8/7)
1113 TCACC
Ksp632I 1281 CTCTTC CTCTTC(1/4)
333 GAAGAG
MslI 44 CATGTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
524 CACATTCATG
MwoI 394 GCAGCAATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
878 GCATAATTGGC
NlaIV 475 GGTGCC GG[ACGT]^[ACGT]CC
1326 GGCACC
NspI 707 ACATGC [GA]CATG^[CT]
939 GCATGC
PstI 356 CTGCAG CTGCA^G
392 CTGCAG
RsaI 1001 GTAC GT^AC
1221 GTAC
SmlI 570 CTCGAG C^T[CT][GA]AG
944 CTCAAG
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AccI AluI BbvI BfaI BmyI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI DpnII EcoRII Fnu4HI FokI
HinfI ItaI MaeI MaeIII MboI
MboII MnlI MseI MvaI NdeII
NlaIII Sau3AI ScrFI SfaNI SfcI
TfiI Tru9I TseI Tsp509I TspRI