(Circular) (Six-base) MAPSORT of: pFN21ASDA1253.seq Check: 1261 from: 1 to: 6088 pFN21ASDA1253 6088 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2486 2486 Size: 6088 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3898 278 Size: 53 83 186 936 2362 2468 Fragments arranged by size: 2468 2362 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2676 2829 2893 3431 1937 Size: 739 153 64 538 4594 Fragments arranged by size: 4594 739 538 153 64 Acc65I G'GTAC_C Cuts at: 3409 3409 Size: 6088 AclI AA'CG_TT Cuts at: 1563 4215 4588 1563 Size: 2652 373 3063 Fragments arranged by size: 3063 2652 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2799 4151 5535 5803 1632 Size: 1167 1352 1384 268 1917 Fragments arranged by size: 1917 1384 1352 1167 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5891 AflIII A'CryG_T Cuts at: 1203 4987 5964 1203 Size: 3784 977 1327 Fragments arranged by size: 3784 1327 977 AgeI A'CCGG_T Cuts at: 4919 4919 Size: 6088 AhdI GACnn_n'nnGTC Cuts at: 4817 4817 Size: 6088 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6088 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6088 AlwNI CAG_nnn'CTG Cuts at: 3831 5403 3831 Size: 1572 4516 ApaLI G'TGCA_C Cuts at: 2384 4145 5301 2384 Size: 1761 1156 3171 Fragments arranged by size: 3171 1761 1156 ApoI r'AATT_y Cuts at: 1493 2408 3397 3623 3775 5884 1493 Size: 915 989 226 152 2109 1697 Fragments arranged by size: 2109 1697 989 915 226 152 AseI AT'TA_AT Cuts at: 160 1032 3080 4642 160 Size: 872 2048 1562 1606 Fragments arranged by size: 2048 1606 1562 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6088 AvaI C'yCGr_G Cuts at: 1870 1943 2593 3413 1870 Size: 73 650 820 4545 Fragments arranged by size: 4545 820 650 73 BamHI G'GATC_C Cuts at: 3418 3785 3418 Size: 367 5721 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2498 3349 3409 4864 618 Size: 325 217 627 711 851 60 1455 1842 Fragments arranged by size: 1842 1455 851 711 627 325 217 60 BanII G_rGCy'C Cuts at: 1391 3407 1391 Size: 2016 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2805 928 Size: 971 906 4211 Fragments arranged by size: 4211 971 906 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4279 4313 1903 Size: 34 2342 34 3678 Fragments arranged by size: 3678 2342 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2055 3980 5190 2055 Size: 1925 1210 2953 Fragments arranged by size: 2953 1925 1210 BclI T'GATC_A Cuts at: 1548 2346 1548 Size: 798 5290 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4699 136 Size: 107 122 71 631 605 3027 1525 Fragments arranged by size: 3027 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2795 6083 2795 Size: 3288 2800 BlpI GC'TnA_GC Cuts at: 3505 3505 Size: 6088 Bme1580I G_kGCm'C Cuts at: 1790 2086 2388 4149 5305 1790 Size: 296 302 1761 1156 2573 Fragments arranged by size: 2573 1761 1156 302 296 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6088 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3819 4777 442 Size: 416 53 83 399 24 2402 958 1753 Fragments arranged by size: 2402 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6088 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3137 4748 1239 Size: 1898 1611 2579 Fragments arranged by size: 2579 1898 1611 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2952 4213 5078 5376 2952 Size: 1261 865 298 3664 Fragments arranged by size: 3664 1261 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4751 882 Size: 617 3252 2219 Fragments arranged by size: 3252 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3385 3879 493 Size: 711 2181 494 2702 Fragments arranged by size: 2702 2181 711 494 BsaBI GATnn'nnATC Cuts at: 3320 6082 3320 Size: 2762 3326 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3895 4277 4912 275 Size: 53 83 186 936 2362 382 635 1451 Fragments arranged by size: 2362 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4521 4919 5193 5340 5751 4521 Size: 398 274 147 411 4858 Fragments arranged by size: 4858 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2160 2190 1178 Size: 30 165 30 757 30 5076 Fragments arranged by size: 5076 757 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2726 1186 Size: 1540 4548 BseYI C'CCAG_C Cuts at: 5291 5787 5291 Size: 496 5592 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5515 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3538 4299 5327 1540 Size: 275 186 1537 761 1028 2301 Fragments arranged by size: 2301 1537 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2048 2388 4149 4234 5305 2048 Size: 340 1761 85 1071 2831 Fragments arranged by size: 2831 1761 1071 340 85 BsmI GAATG_Cn' Cuts at: 2441 3599 3692 2441 Size: 1158 93 4837 Fragments arranged by size: 4837 1158 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2048 2086 2388 3407 4149 4234 5305 Size: 399 258 38 302 1019 742 85 1071 Cuts at: 5305 1391 Size: 2174 Fragments arranged by size: 2174 1071 1019 742 399 302 258 85 38 BspEI T'CCGG_A Cuts at: 5751 5751 Size: 6088 BspHI T'CATG_A Cuts at: 3222 3977 5707 3222 Size: 755 1730 3603 Fragments arranged by size: 3603 1730 755 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2486 3443 844 Size: 1642 957 3489 Fragments arranged by size: 3489 1642 957 BsrBI CCG'CTC Cuts at: 1394 1823 3975 1394 Size: 429 2152 3507 Fragments arranged by size: 3507 2152 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4583 4757 65 Size: 1183 3335 174 1396 Fragments arranged by size: 3335 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4732 4919 1700 Size: 110 2922 187 2869 Fragments arranged by size: 2922 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6088 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6088 BssSI C'ACGA_G Cuts at: 3261 3344 4148 5160 3261 Size: 83 804 1012 4189 Fragments arranged by size: 4189 1012 804 83 BstAPI GCAn_nnn'nTGC Cuts at: 794 2423 794 Size: 1629 4459 BstBI TT'CG_AA Cuts at: 2007 4892 2007 Size: 2885 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6088 BstYI r'GATC_y Cuts at: 1972 2795 3418 3785 4170 4187 5639 5725 5737 Size: 823 623 367 385 17 1452 86 12 Cuts at: 5737 6083 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 823 623 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2049 513 Size: 552 957 27 4552 Fragments arranged by size: 4552 957 552 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5349 Fragments arranged by size: 5349 683 56 BtsI GCAGTG_nn' Cuts at: 2371 2388 3604 4398 4418 2371 Size: 17 1216 794 20 4041 Fragments arranged by size: 4041 1216 794 20 17 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6088 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6053 DraI TTT'AAA Cuts at: 2407 3392 2407 Size: 985 5103 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5095 809 Size: 507 3779 1802 Fragments arranged by size: 3779 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3535 4424 6053 Size: 54 1425 50 275 18 1705 889 1629 Cuts at: 6053 8 Size: 43 Fragments arranged by size: 1705 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3535 1537 Size: 275 1723 4090 Fragments arranged by size: 4090 1723 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2351 3310 4018 1353 Size: 998 959 708 3423 Fragments arranged by size: 3423 998 959 708 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4675 5049 5195 387 Size: 1064 138 3086 374 146 1280 Fragments arranged by size: 3086 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2799 3137 4151 4748 5535 5803 1239 Size: 393 1167 338 1014 597 787 268 1524 Fragments arranged by size: 1524 1167 1014 787 597 393 338 268 EcoRI G'AATT_C Cuts at: 3397 3775 3397 Size: 378 5710 FspI TGC'GCA Cuts at: 4594 4594 Size: 6088 HaeII r_GCGC'y Cuts at: 1678 5235 1678 Size: 3557 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2973 3005 3259 3291 4729 Size: 32 135 32 1401 32 254 32 1438 Cuts at: 4729 4761 4803 4835 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1438 1401 254 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2728 3432 3678 669 Size: 988 281 790 704 246 3079 Fragments arranged by size: 3079 988 790 704 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6088 HpaI GTT'AAC Cuts at: 3678 3678 Size: 6088 KpnI G_GTAC'C Cuts at: 3413 3413 Size: 6088 MfeI C'AATT_G Cuts at: 2421 3687 2421 Size: 1266 4822 MluI A'CGCG_T Cuts at: 5964 5964 Size: 6088 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2146 3293 5202 5386 1305 Size: 841 1147 1909 184 2007 Fragments arranged by size: 2007 1909 1147 841 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4266 Fragments arranged by size: 4266 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2071 2551 4046 4405 4564 518 Size: 648 905 480 1495 359 159 2042 Fragments arranged by size: 2042 1495 905 648 480 359 159 MspA1I CmG'CkG Cuts at: 1606 3287 3504 4181 5329 5574 1606 Size: 1681 217 677 1148 245 2120 Fragments arranged by size: 2120 1681 1148 677 245 217 NcoI C'CATG_G Cuts at: 513 1065 2022 2049 513 Size: 552 957 27 4552 Fragments arranged by size: 4552 957 552 27 NdeI CA'TA_TG Cuts at: 387 387 Size: 6088 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6088 NotI GC'GGCC_GC Cuts at: 3535 3535 Size: 6088 NspI r_CATG'y Cuts at: 1132 1229 2734 2966 3446 4991 1132 Size: 97 1505 232 480 1545 2229 Fragments arranged by size: 2229 1545 1505 480 232 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6088 PciI A'CATG_T Cuts at: 4987 4987 Size: 6088 PflMI CCAn_nnn'nTGG Cuts at: 1108 2798 1108 Size: 1690 4398 PfoI T'CCnGG_A Cuts at: 2227 2227 Size: 6088 PmeI GTTT'AAAC Cuts at: 3392 3392 Size: 6088 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4157 4189 5670 5702 4157 Size: 32 1481 32 4543 Fragments arranged by size: 4543 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3287 1613 Size: 1674 4414 PsiI TTA'TAA Cuts at: 3658 3658 Size: 6088 PstI C_TGCA'G Cuts at: 830 2383 2419 3440 830 Size: 1553 36 1021 3478 Fragments arranged by size: 3478 1553 1021 36 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6088 PvuII CAG'CTG Cuts at: 3287 3287 Size: 6088 SalI G'TCGA_C Cuts at: 1936 3430 1936 Size: 1494 4594 SbfI CC_TGCA'GG Cuts at: 3440 3440 Size: 6088 ScaI AGT'ACT Cuts at: 4336 6048 4336 Size: 1712 4376 SfcI C'TryA_G Cuts at: 826 1045 2379 2415 2625 3144 3436 4571 5252 Size: 219 1334 36 210 519 292 1135 681 Cuts at: 5252 5443 826 Size: 191 1471 Fragments arranged by size: 1471 1334 1135 681 519 292 219 210 191 36 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6088 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6088 SmaI CCC'GGG Cuts at: 1872 3415 1872 Size: 1543 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2593 2967 4192 5093 5355 820 Size: 197 926 650 374 1225 901 262 1553 Fragments arranged by size: 1553 1225 926 901 650 374 262 197 SnaBI TAC'GTA Cuts at: 493 3385 3879 493 Size: 2892 494 2702 Fragments arranged by size: 2892 2702 494 SpeI A'CTAG_T Cuts at: 152 2524 152 Size: 2372 3716 SphI G_CATG'C Cuts at: 1132 2966 3446 1132 Size: 1834 480 3774 Fragments arranged by size: 3774 1834 480 SspI AAT'ATT Cuts at: 5 52 4012 5 Size: 47 3960 2081 Fragments arranged by size: 3960 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6088 StyI C'CwwG_G Cuts at: 513 1065 2022 2049 3527 513 Size: 552 957 27 1478 3074 Fragments arranged by size: 3074 1478 957 552 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3283 4115 4132 4285 4470 4915 325 Size: 1255 1703 832 17 153 185 445 1498 Fragments arranged by size: 1703 1498 1255 832 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4334 6046 96 Size: 274 80 33 51 1443 2357 1712 138 Fragments arranged by size: 2357 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6048 6080 6048 Size: 32 6056 Tth111I GACn'n_nGTC Cuts at: 1316 2848 1316 Size: 1532 4556 XbaI T'CTAG_A Cuts at: 3424 3424 Size: 6088 XhoI C'TCGA_G Cuts at: 1943 2593 1943 Size: 650 5438 XmaI C'CCGG_G Cuts at: 1870 3413 1870 Size: 1543 4545 XmnI GAAnn'nnTTC Cuts at: 4217 4217 Size: 6088 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3896 276 Size: 53 83 186 936 2362 2468 Fragments arranged by size: 2468 2362 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI