Restriction map for
pFN21ASDA1253
(Linear) (Six-base) MAPSORT of: KIAA1253.seq Check: 5507 from: 1 to: 1359
KIAA1253.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 463 1359
Size: 463 896
AccI GT'mk_AC
Cuts at: 0 653 806 870 1359
Size: 653 153 64 489
Fragments arranged by size:
653 489 153 64
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 776 1359
Size: 776 583
ApaLI G'TGCA_C
Cuts at: 0 361 1359
Size: 361 998
ApoI r'AATT_y
Cuts at: 0 385 1359
Size: 385 974
AseI AT'TA_AT
Cuts at: 0 1057 1359
Size: 1057 302
AvaI C'yCGr_G
Cuts at: 0 570 1359
Size: 570 789
BanI G'GyrC_C
Cuts at: 0 475 1326 1359
Size: 475 851 33
Fragments arranged by size:
851 475 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 782 1359
Size: 782 577
BciVI GTATCCnnnnn_n'
Cuts at: 0 32 1359
Size: 32 1327
BclI T'GATC_A
Cuts at: 0 323 1359
Size: 323 1036
BglII A'GATC_T
Cuts at: 0 772 1359
Size: 772 587
Bme1580I G_kGCm'C
Cuts at: 0 63 365 1359
Size: 63 302 994
Fragments arranged by size:
994 302 63
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1114 1359
Size: 1114 245
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 929 1359
Size: 929 430
BsaBI GATnn'nnATC
Cuts at: 0 1297 1359
Size: 1297 62
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 137 167 1359
Size: 137 30 1192
Fragments arranged by size:
1192 137 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 703 1359
Size: 703 656
BsiHKAI G_wGCw'C
Cuts at: 0 25 365 1359
Size: 25 340 994
Fragments arranged by size:
994 340 25
BsmI GAATG_Cn'
Cuts at: 0 418 1359
Size: 418 941
Bsp1286I G_dGCh'C
Cuts at: 0 25 63 365 1359
Size: 25 38 302 994
Fragments arranged by size:
994 302 38 25
BspHI T'CATG_A
Cuts at: 0 1199 1359
Size: 1199 160
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 463 1359
Size: 463 896
BssSI C'ACGA_G
Cuts at: 0 1238 1321 1359
Size: 1238 83 38
Fragments arranged by size:
1238 83 38
BstAPI GCAn_nnn'nTGC
Cuts at: 0 400 1359
Size: 400 959
BstYI r'GATC_y
Cuts at: 0 772 1359
Size: 772 587
BtgI C'CryG_G
Cuts at: 0 26 1359
Size: 26 1333
BtsI GCAGTG_nn'
Cuts at: 0 348 365 1359
Size: 348 17 994
Fragments arranged by size:
994 348 17
DraI TTT'AAA
Cuts at: 0 384 1359
Size: 384 975
EarI CTCTTCn'nnn_
Cuts at: 0 328 1287 1359
Size: 328 959 72
Fragments arranged by size:
959 328 72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 776 1114 1359
Size: 776 338 245
Fragments arranged by size:
776 338 245
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 950 982 1236 1268 1359
Size: 950 32 254 32 91
Fragments arranged by size:
950 254 91 32 32
HincII GTy'rAC
Cuts at: 0 705 1359
Size: 705 654
MfeI C'AATT_G
Cuts at: 0 398 1359
Size: 398 961
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 123 1270 1359
Size: 123 1147 89
Fragments arranged by size:
1147 123 89
MslI CAynn'nnrTG
Cuts at: 0 48 528 1359
Size: 48 480 831
Fragments arranged by size:
831 480 48
MspA1I CmG'CkG
Cuts at: 0 1264 1359
Size: 1264 95
NcoI C'CATG_G
Cuts at: 0 26 1359
Size: 26 1333
NspI r_CATG'y
Cuts at: 0 711 943 1359
Size: 711 232 416
Fragments arranged by size:
711 416 232
PflMI CCAn_nnn'nTGG
Cuts at: 0 775 1359
Size: 775 584
PfoI T'CCnGG_A
Cuts at: 0 204 1359
Size: 204 1155
PshAI GACnn'nnGTC
Cuts at: 0 1264 1359
Size: 1264 95
PstI C_TGCA'G
Cuts at: 0 360 396 1359
Size: 360 36 963
Fragments arranged by size:
963 360 36
PvuII CAG'CTG
Cuts at: 0 1264 1359
Size: 1264 95
SfcI C'TryA_G
Cuts at: 0 356 392 602 1121 1359
Size: 356 36 210 519 238
Fragments arranged by size:
519 356 238 210 36
SmlI C'TyrA_G
Cuts at: 0 570 944 1359
Size: 570 374 415
Fragments arranged by size:
570 415 374
SpeI A'CTAG_T
Cuts at: 0 501 1359
Size: 501 858
SphI G_CATG'C
Cuts at: 0 943 1359
Size: 943 416
StyI C'CwwG_G
Cuts at: 0 26 1359
Size: 26 1333
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1260 1359
Size: 1260 99
Tth111I GACn'n_nGTC
Cuts at: 0 825 1359
Size: 825 534
XhoI C'TCGA_G
Cuts at: 0 570 1359
Size: 570 789
Enzymes that do cut:
AarI AccI AcuI ApaLI ApoI AseI AvaI BanI
BbsI BciVI BclI BglII Bme1580I BpmI BpuEI BsaBI
BsaXI BseRI BsiHKAI BsmI Bsp1286I BspHI BspMI BssSI
BstAPI BstYI BtgI BtsI DraI EarI Eco57MI Hin4I
HincII MfeI MmeI MslI MspA1I NcoI NspI PflMI
PfoI PshAI PstI PvuII SfcI SmlI SpeI SphI
StyI TaqII Tth111I XhoI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI AscI AsiSI AvrII
BaeI BamHI BanII BbeI BbvCI BcgI BfrBI BglI
BlpI BmgBI BmrI BmtI BplI Bpu10I BsaI BsaAI
BsaHI BsaWI BseYI BsgI BsiEI BsiWI BsmBI BspEI
BsrBI BsrDI BsrFI BsrGI BssHII BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI EaeI
EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HaeII HindIII HpaI KasI KpnI
MluI MscI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PmeI PmlI PpiI
PpuMI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SrfI SspI StuI
SwaI TatI TstI XbaI XcmI XmaI XmnI ZraI