Restriction map for
pFN21ASDA1246
##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AflIII AgeI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AvrII BbrPI BcgI
BciVI BclI BfrI BglII BlnI
BsaAI BsaBI BsaWI BseAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssHII Bst1107I
BstBI BstZ17I Bsu36I ClaI DraI
DraIII DrdI Eco47III Eco81I EcoRI
EcoRV EcoT22I FbaI FseI HindIII
HpaI KpnI KspI MamI MluI
MroI MseI NaeI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI SacII SapI
SbfI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI SwaI
Tru9I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AatII 2055 GACGTC GACGT^C
AccI 52 GTCGAC GT^[AC][GT]AC
AhdI 509 GACGCATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 509 GACGCATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 243 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1811 TGCGCA TGC^GCA
BamHI 1922 GGATCC G^GATCC
BbeI 1741 GGCGCC GGCGC^C
BbsI 1642 GTCTTC GAAGAC(2/6)
BbvCI 1143 CCTCAGC CCTCAGC(-5/-2)
BfaI 25 CTAG C^TAG
BlpI 531 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 531 GCTGAGC GC^T[ACGT]AGC
BpuAI 1642 GTCTTC GAAGAC(2/6)
BsgI 1166 CTGCAC GTGCAG(16/14)
BsiEI 1709 CGGCCG CG[GA][CT]^CG
BsrFI 152 ACCGGC [GA]^CCGG[CT]
BssSI 1684 CACGAG CACGAG(-5/-1)
BstAPI 627 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 721 GGTAACC G^GT[ACGT]ACC
BstPI 721 GGTAACC G^GT[ACGT]ACC
CelII 531 GCTGAGC GC^T[ACGT]AGC
Cfr10I 152 ACCGGC [GA]^CCGG[CT]
CpoI 2078 CGGACCG CG^G[AT]CCG
EagI 1709 CGGCCG C^GGCCG
Eam1105I 509 GACGCATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1863 CTCTTC CTCTTC(1/4)
EclXI 1709 CGGCCG C^GGCCG
Eco52I 1709 CGGCCG C^GGCCG
EcoNI 342 CCTTGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 721 GGTAACC G^GT[ACGT]ACC
FspI 1811 TGCGCA TGC^GCA
HaeII 1741 GGCGCC [GA]GCGC^[CT]
KasI 1741 GGCGCC G^GCGCC
Ksp632I 1863 CTCTTC CTCTTC(1/4)
MaeI 25 CTAG C^TAG
MaeII 2056 ACGT A^CGT
MfeI 738 CAATTG C^AATTG
MunI 738 CAATTG C^AATTG
NarI 1741 GGCGCC GG^CGCC
NheI 24 GCTAGC G^CTAGC
PflFI 243 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1005 GACCGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 2078 CGGACCG CG^G[AT]CCG
SacI 1819 GAGCTC GAGCT^C
SalI 52 GTCGAC G^TCGAC
ScaI 68 AGTACT AGT^ACT
SfoI 1741 GGCGCC GGC^GCC
SphI 2282 GCATGC GCATG^C
StuI 1421 AGGCCT AGG^CCT
Tsp509I 739 AATT ^AATT
Tth111I 243 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccII 1849 CGCG CG^CG
1940 CGCG
AspHI 1819 GAGCTC G[AT]GC[AT]^C
2358 GAGCAC
BalI 1475 TGGCCA TGG^CCA
1628 TGGCCA
BsiHKAI 1819 GAGCTC G[AT]GC[AT]^C
2358 GAGCAC
BsrDI 1077 CATTGC GCAATG(2/0)
1718 GCAATG
BstUI 1849 CGCG CG^CG
1940 CGCG
HgaI 55 GACGC GACGC(5/10)
509 GACGC
MluNI 1475 TGGCCA TGG^CCA
1628 TGGCCA
MscI 1475 TGGCCA TGG^CCA
1628 TGGCCA
MvnI 1849 CGCG CG^CG
1940 CGCG
NcoI 483 CCATGG C^CATGG
1109 CCATGG
NspI 831 GCATGT [GA]CATG^[CT]
2282 GCATGC
PaeR7I 295 CTCGAG C^TCGAG
766 CTCGAG
PflMI 337 CCAAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1581 CCAGATTCTGG
PpuMI 103 GGGACCC [GA]G^G[AT]CC[CT]
941 GGGACCC
SexAI 1439 ACCTGGT A^CC[AT]GGT
2309 ACCTGGT
TfiI 84 GAATC G^A[AT]TC
1584 GATTC
Van91I 337 CCAAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1581 CCAGATTCTGG
XhoI 295 CTCGAG C^TCGAG
766 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI ApaI AvaI AvaII BanI
BanII BbvI BcnI BglI BmyI
BpmI BsaHI BsaI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StyI
TaqI TseI Tsp45I TspRI TthHB8I
XcmI XhoII XmaCI XmaI