Restriction map for
pFN21ASDA1246
(Linear) (Six-base) MAPSORT of: KIAA1246.seq Check: 6654 from: 1 to: 2367
KIAA1246.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1081 2367
Size: 1081 1286
AatII G_ACGT'C
Cuts at: 0 2059 2367
Size: 2059 308
AccI GT'mk_AC
Cuts at: 0 53 2367
Size: 53 2314
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1307 2367
Size: 1307 1060
AhdI GACnn_n'nnGTC
Cuts at: 0 514 2367
Size: 514 1853
AleI CACnn'nnGTG
Cuts at: 0 924 2367
Size: 924 1443
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 349 2305 2367
Size: 349 1956 62
Fragments arranged by size:
1956 349 62
AlwNI CAG_nnn'CTG
Cuts at: 0 87 594 633 1453 1587 2367
Size: 87 507 39 820 134 780
Fragments arranged by size:
820 780 507 134 87 39
ApaI G_GGCC'C
Cuts at: 0 1782 1795 1914 2102 2322 2367
Size: 1782 13 119 188 220 45
Fragments arranged by size:
1782 220 188 119 45 13
AvaI C'yCGr_G
Cuts at: 0 295 766 1340 2063 2111 2321 2367
Size: 295 471 574 723 48 210 46
Fragments arranged by size:
723 574 471 295 210 48 46
BamHI G'GATC_C
Cuts at: 0 1922 2367
Size: 1922 445
BanI G'GyrC_C
Cuts at: 0 571 1024 1063 1594 1741 2367
Size: 571 453 39 531 147 626
Fragments arranged by size:
626 571 531 453 147 39
BanII G_rGCy'C
Cuts at: 0 1782 1795 1823 1914 2102 2322 2367
Size: 1782 13 28 91 188 220 45
Fragments arranged by size:
1782 220 188 91 45 28 13
BbeI G_GCGC'C
Cuts at: 0 1745 2367
Size: 1745 622
BbsI GAAGACnn'nnnn_
Cuts at: 0 1635 2367
Size: 1635 732
BbvCI CC'TCA_GC
Cuts at: 0 1144 2367
Size: 1144 1223
BglI GCCn_nnn'nGGC
Cuts at: 0 179 1099 1843 2367
Size: 179 920 744 524
Fragments arranged by size:
920 744 524 179
BlpI GC'TnA_GC
Cuts at: 0 532 2367
Size: 532 1835
Bme1580I G_kGCm'C
Cuts at: 0 115 574 1782 1795 1914 2102 2322 2367
Size: 115 459 1208 13 119 188 220 45
Fragments arranged by size:
1208 459 220 188 119 115 45 13
BmrI ACTGGGnnnn_n'
Cuts at: 0 109 972 1527 2367
Size: 109 863 555 840
Fragments arranged by size:
863 840 555 109
BmtI G_CTAG'C
Cuts at: 0 28 2367
Size: 28 2339
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 922 1188 1854 1938 2010 2018 2367
Size: 922 266 666 84 72 8 349
Fragments arranged by size:
922 666 349 266 84 72 8
Bpu10I CC'TnA_GC
Cuts at: 0 1144 2367
Size: 1144 1223
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 796 907 984 1978 2367
Size: 796 111 77 994 389
Fragments arranged by size:
994 796 389 111 77
BsaI GGTCTCn'nnnn_
Cuts at: 0 1125 2247 2367
Size: 1125 1122 120
Fragments arranged by size:
1125 1122 120
BsaHI Gr'CG_yC
Cuts at: 0 56 1742 2056 2367
Size: 56 1686 314 311
Fragments arranged by size:
1686 314 311 56
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 838 868 2195 2225 2367
Size: 838 30 1327 30 142
Fragments arranged by size:
1327 838 142 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 856 859 987 1851 2026 2222 2314 2367
Size: 856 3 128 864 175 196 92 53
Fragments arranged by size:
864 856 196 175 128 92 53 3
BseYI C'CCAG_C
Cuts at: 0 405 875 946 1407 1693 1745 1902 1951
Size: 405 470 71 461 286 52 157 49
Cuts at: 1951 2050 2094 2367
Size: 99 44 273
Fragments arranged by size:
470 461 405 286 273 157 99 71
52 49 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1151 2367
Size: 1151 1216
BsiEI CG_ry'CG
Cuts at: 0 1712 2367
Size: 1712 655
BsiHKAI G_wGCw'C
Cuts at: 0 1823 2362 2367
Size: 1823 539 5
Fragments arranged by size:
1823 539 5
Bsp1286I G_dGCh'C
Cuts at: 0 115 574 1782 1795 1823 1914 2102 2322
Size: 115 459 1208 13 28 91 188 220
Cuts at: 2322 2362 2367
Size: 40 5
Fragments arranged by size:
1208 459 220 188 115 91 40 28
13 5
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 383 557 593 1081 2367
Size: 383 174 36 488 1286
Fragments arranged by size:
1286 488 383 174 36
BsrBI CCG'CTC
Cuts at: 0 308 659 771 2367
Size: 308 351 112 1596
Fragments arranged by size:
1596 351 308 112
BsrDI GCAATG_nn'
Cuts at: 0 1076 1725 2367
Size: 1076 649 642
Fragments arranged by size:
1076 649 642
BsrFI r'CCGG_y
Cuts at: 0 152 2367
Size: 152 2215
BssSI C'ACGA_G
Cuts at: 0 1684 2367
Size: 1684 683
BstAPI GCAn_nnn'nTGC
Cuts at: 0 633 2367
Size: 633 1734
BstEII G'GTnAC_C
Cuts at: 0 721 2367
Size: 721 1646
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1110 1521 1605 1638 1701 1878 2367
Size: 1110 411 84 33 63 177 489
Fragments arranged by size:
1110 489 411 177 84 63 33
BstYI r'GATC_y
Cuts at: 0 456 606 1922 2367
Size: 456 150 1316 445
Fragments arranged by size:
1316 456 445 150
BtgI C'CryG_G
Cuts at: 0 47 483 493 1103 1109 2367
Size: 47 436 10 610 6 1258
Fragments arranged by size:
1258 610 436 47 10 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 399 546 2367
Size: 399 147 1821
Fragments arranged by size:
1821 399 147
BtsI GCAGTG_nn'
Cuts at: 0 732 1163 1239 1567 1890 2089 2367
Size: 732 431 76 328 323 199 278
Fragments arranged by size:
732 431 328 323 278 199 76
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 672 707 2367
Size: 672 35 1660
Fragments arranged by size:
1660 672 35
EaeI y'GGCC_r
Cuts at: 0 914 1106 1475 1508 1628 1709 2367
Size: 914 192 369 33 120 81 658
Fragments arranged by size:
914 658 369 192 120 81 33
EagI C'GGCC_G
Cuts at: 0 1709 2367
Size: 1709 658
EarI CTCTTCn'nnn_
Cuts at: 0 1869 2367
Size: 1869 498
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 171 1256 1923 2367
Size: 171 1085 667 444
Fragments arranged by size:
1085 667 444 171
EcoICRI GAG'CTC
Cuts at: 0 1821 2367
Size: 1821 546
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 922 1188 1307 1854 1938 2010 2018 2367
Size: 922 266 119 547 84 72 8 349
Fragments arranged by size:
922 547 349 266 119 84 72 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 346 2367
Size: 346 2021
EcoO109I rG'GnC_Cy
Cuts at: 0 104 363 807 942 1689 1778 1779 1791
Size: 104 259 444 135 747 89 1 12
Cuts at: 1791 1911 1973 2098 2099 2318 2367
Size: 120 62 125 1 219 49
Fragments arranged by size:
747 444 259 219 135 125 120 104
89 62 49 12 1 1
FspI TGC'GCA
Cuts at: 0 1813 2367
Size: 1813 554
HaeII r_GCGC'y
Cuts at: 0 1745 2367
Size: 1745 622
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 500 532 598 630 2367
Size: 500 32 66 32 1737
Fragments arranged by size:
1737 500 66 32 32
HincII GTy'rAC
Cuts at: 0 54 372 519 1434 2277 2367
Size: 54 318 147 915 843 90
Fragments arranged by size:
915 843 318 147 90 54
KasI G'GCGC_C
Cuts at: 0 1741 2367
Size: 1741 626
MfeI C'AATT_G
Cuts at: 0 738 2367
Size: 738 1629
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1439 2120 2367
Size: 1439 681 247
Fragments arranged by size:
1439 681 247
MscI TGG'CCA
Cuts at: 0 1477 1630 2367
Size: 1477 153 737
Fragments arranged by size:
1477 737 153
MslI CAynn'nnrTG
Cuts at: 0 836 924 1108 1624 1657 2367
Size: 836 88 184 516 33 710
Fragments arranged by size:
836 710 516 184 88 33
MspA1I CmG'CkG
Cuts at: 0 405 531 1009 1134 1359 1708 2367
Size: 405 126 478 125 225 349 659
Fragments arranged by size:
659 478 405 349 225 126 125
NarI GG'CG_CC
Cuts at: 0 1742 2367
Size: 1742 625
NcoI C'CATG_G
Cuts at: 0 483 1109 2367
Size: 483 626 1258
Fragments arranged by size:
1258 626 483
NheI G'CTAG_C
Cuts at: 0 24 2367
Size: 24 2343
NspI r_CATG'y
Cuts at: 0 835 2286 2367
Size: 835 1451 81
Fragments arranged by size:
1451 835 81
PasI CC'CwG_GG
Cuts at: 0 802 1878 2367
Size: 802 1076 489
Fragments arranged by size:
1076 802 489
PflMI CCAn_nnn'nTGG
Cuts at: 0 343 1587 2367
Size: 343 1244 780
Fragments arranged by size:
1244 780 343
PfoI T'CCnGG_A
Cuts at: 0 250 1823 2026 2367
Size: 250 1573 203 341
Fragments arranged by size:
1573 341 250 203
PpuMI rG'GwC_Cy
Cuts at: 0 104 942 2367
Size: 104 838 1425
Fragments arranged by size:
1425 838 104
PshAI GACnn'nnGTC
Cuts at: 0 1009 2367
Size: 1009 1358
PspOMI G'GGCC_C
Cuts at: 0 1778 1791 1910 2098 2318 2367
Size: 1778 13 119 188 220 49
Fragments arranged by size:
1778 220 188 119 49 13
PspXI vC'TCGA_Gb
Cuts at: 0 766 2367
Size: 766 1601
PstI C_TGCA'G
Cuts at: 0 380 590 629 1364 2367
Size: 380 210 39 735 1003
Fragments arranged by size:
1003 735 380 210 39
PvuII CAG'CTG
Cuts at: 0 405 531 1134 1359 2367
Size: 405 126 603 225 1008
Fragments arranged by size:
1008 603 405 225 126
RsrII CG'GwC_CG
Cuts at: 0 2079 2367
Size: 2079 288
SacI G_AGCT'C
Cuts at: 0 1823 2367
Size: 1823 544
SalI G'TCGA_C
Cuts at: 0 52 2367
Size: 52 2315
SanDI GG'GwC_CC
Cuts at: 0 104 942 2367
Size: 104 838 1425
Fragments arranged by size:
1425 838 104
ScaI AGT'ACT
Cuts at: 0 70 2367
Size: 70 2297
SexAI A'CCwGG_T
Cuts at: 0 1439 2309 2367
Size: 1439 870 58
Fragments arranged by size:
1439 870 58
SfcI C'TryA_G
Cuts at: 0 376 586 625 1360 2223 2367
Size: 376 210 39 735 863 144
Fragments arranged by size:
863 735 376 210 144 39
SfoI GGC'GCC
Cuts at: 0 1743 2367
Size: 1743 624
SmaI CCC'GGG
Cuts at: 0 1342 2065 2113 2323 2367
Size: 1342 723 48 210 44
Fragments arranged by size:
1342 723 210 48 44
SmlI C'TyrA_G
Cuts at: 0 295 766 811 922 999 1993 2367
Size: 295 471 45 111 77 994 374
Fragments arranged by size:
994 471 374 295 111 77 45
SphI G_CATG'C
Cuts at: 0 2286 2367
Size: 2286 81
SrfI GCCC'GGGC
Cuts at: 0 2113 2367
Size: 2113 254
StuI AGG'CCT
Cuts at: 0 1423 2367
Size: 1423 944
StyI C'CwwG_G
Cuts at: 0 119 342 483 1109 1544 2367
Size: 119 223 141 626 435 823
Fragments arranged by size:
823 626 435 223 141 119
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 889 2096 2337 2367
Size: 889 1207 241 30
Fragments arranged by size:
1207 889 241 30
TatI w'GTAC_w
Cuts at: 0 68 1364 1724 2367
Size: 68 1296 360 643
Fragments arranged by size:
1296 643 360 68
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 838 870 2195 2227 2367
Size: 838 32 1325 32 140
Fragments arranged by size:
1325 838 140 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 246 2367
Size: 246 2121
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1110 1305 1518 2367
Size: 1110 195 213 849
Fragments arranged by size:
1110 849 213 195
XhoI C'TCGA_G
Cuts at: 0 295 766 2367
Size: 295 471 1601
Fragments arranged by size:
1601 471 295
XmaI C'CCGG_G
Cuts at: 0 1340 2063 2111 2321 2367
Size: 1340 723 48 210 46
Fragments arranged by size:
1340 723 210 48 46
ZraI GAC'GTC
Cuts at: 0 2057 2367
Size: 2057 310
Enzymes that do cut:
AarI AatII AccI AcuI AhdI AleI AlfI AlwNI
ApaI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BglI BlpI Bme1580I BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII
BstXI BstYI BtgI BtgZI BtsI CspCI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII
Hin4I HincII KasI MfeI MmeI MscI MslI MspA1I
NarI NcoI NheI NspI PasI PflMI PfoI PpuMI
PshAI PspOMI PspXI PstI PvuII RsrII SacI SalI
SanDI ScaI SexAI SfcI SfoI SmaI SmlI SphI
SrfI StuI StyI TaqII TatI TstI Tth111I XcmI
XhoI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AgeI AloI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BcgI BciVI
BclI BfrBI BglII BmgBI BplI BsaAI BsaBI BsaWI
BsiWI BsmI BsmBI BspEI BspHI BsrGI BssHII BstBI
BstZ17I Bsu36I ClaI DraI DraIII DrdI EcoRI EcoRV
FalI FseI FspAI HindIII HpaI KpnI MluI NaeI
NdeI NgoMIV NotI NruI NsiI PacI PciI PmeI
PmlI PpiI PsiI PsrI PvuI SacII SapI SbfI
SfiI SgfI SgrAI SnaBI SpeI SspI SwaI XbaI
XmnI