(Circular) (Six-base) MAPSORT of: pFN21ASDA1234.seq Check: 874 from: 1 to: 6886 pFN21ASDA1234 6886 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4031 4031 Size: 6886 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4696 278 Size: 53 83 186 936 3160 2468 Fragments arranged by size: 3160 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4229 1937 Size: 2292 4594 Acc65I G'GTAC_C Cuts at: 4207 4207 Size: 6886 AclI AA'CG_TT Cuts at: 1563 5013 5386 1563 Size: 3450 373 3063 Fragments arranged by size: 3450 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4949 6333 6601 1632 Size: 3317 1384 268 1917 Fragments arranged by size: 3317 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6689 AflIII A'CryG_T Cuts at: 1203 5785 6762 1203 Size: 4582 977 1327 Fragments arranged by size: 4582 1327 977 AgeI A'CCGG_T Cuts at: 5717 5717 Size: 6886 AhdI GACnn_n'nnGTC Cuts at: 5615 5615 Size: 6886 AleI CACnn'nnGTG Cuts at: 1166 4078 1166 Size: 2912 3974 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3506 4190 1184 Size: 2322 684 3880 Fragments arranged by size: 3880 2322 684 AlwNI CAG_nnn'CTG Cuts at: 2354 4629 6201 2354 Size: 2275 1572 3039 Fragments arranged by size: 3039 2275 1572 ApaI G_GGCC'C Cuts at: 2641 3142 2641 Size: 501 6385 ApaLI G'TGCA_C Cuts at: 2053 3515 3993 4165 4943 6099 2053 Size: 1462 478 172 778 1156 2840 Fragments arranged by size: 2840 1462 1156 778 478 172 ApoI r'AATT_y Cuts at: 1493 2822 4195 4421 4573 6682 1493 Size: 1329 1373 226 152 2109 1697 Fragments arranged by size: 2109 1697 1373 1329 226 152 AseI AT'TA_AT Cuts at: 160 1032 5440 160 Size: 872 4408 1606 Fragments arranged by size: 4408 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6886 AvaI C'yCGr_G Cuts at: 1870 1943 2731 2745 2783 4109 4211 1870 Size: 73 788 14 38 1326 102 4545 Fragments arranged by size: 4545 1326 788 102 73 38 14 BamHI G'GATC_C Cuts at: 4216 4583 4216 Size: 367 6519 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2315 2848 2899 3320 4105 Size: 325 217 627 528 533 51 421 785 Cuts at: 4105 4207 5662 618 Size: 102 1455 1842 Fragments arranged by size: 1842 1455 785 627 533 528 421 325 217 102 51 BanII G_rGCy'C Cuts at: 1391 2297 2641 3142 3197 3452 3580 3871 3951 Size: 906 344 501 55 255 128 291 80 Cuts at: 3951 4065 4205 1391 Size: 114 140 4072 Fragments arranged by size: 4072 906 501 344 291 255 140 128 114 80 55 BbeI G_GCGC'C Cuts at: 2319 2852 2319 Size: 533 6353 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5915 BbvCI CC'TCA_GC Cuts at: 3567 4035 3567 Size: 468 6418 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3652 3686 5077 5111 1903 Size: 34 1715 34 1391 34 3678 Fragments arranged by size: 3678 1715 1391 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4778 5988 4778 Size: 1210 5676 BclI T'GATC_A Cuts at: 1548 1548 Size: 6886 BfrBI ATG'CAT Cuts at: 2438 2438 Size: 6886 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2111 5497 136 Size: 107 122 71 631 605 439 3386 1525 Fragments arranged by size: 3386 1525 631 605 439 122 107 71 BglII A'GATC_T Cuts at: 6881 6881 Size: 6886 BlpI GC'TnA_GC Cuts at: 3230 3579 3870 4303 3230 Size: 349 291 433 5813 Fragments arranged by size: 5813 433 349 291 Bme1580I G_kGCm'C Cuts at: 1790 2057 2641 2654 3124 3142 3416 3519 3738 Size: 267 584 13 470 18 274 103 219 Cuts at: 3738 3855 3997 4110 4169 4947 6103 1790 Size: 117 142 113 59 778 1156 2573 Fragments arranged by size: 2573 1156 778 584 470 274 267 219 142 117 113 103 59 18 13 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6886 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2560 3453 3961 Size: 416 53 83 399 24 1143 893 508 Cuts at: 3961 4072 4617 5575 442 Size: 111 545 958 1753 Fragments arranged by size: 1753 1143 958 893 545 508 416 399 111 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6886 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2311 2882 3282 3718 3835 2311 Size: 571 400 436 117 5362 Fragments arranged by size: 5362 571 436 400 117 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2384 3252 3264 3454 3594 3965 5546 1239 Size: 1145 868 12 190 140 371 1581 2579 Fragments arranged by size: 2579 1581 1145 868 371 190 140 12 Bpu10I CC'TnA_GC Cuts at: 2633 3092 3196 3567 4035 2633 Size: 459 104 371 468 5484 Fragments arranged by size: 5484 468 459 371 104 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5011 5876 6174 5011 Size: 865 298 5723 Fragments arranged by size: 5723 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5549 882 Size: 617 4050 2219 Fragments arranged by size: 4050 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2058 2515 4183 4677 493 Size: 711 854 457 1668 494 2702 Fragments arranged by size: 2702 1668 854 711 494 457 BsaBI GATnn'nnATC Cuts at: 6880 6880 Size: 6886 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2316 2849 4693 5075 Size: 53 83 186 936 783 533 1844 382 Cuts at: 5075 5710 275 Size: 635 1451 Fragments arranged by size: 1844 1451 936 783 635 533 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5319 5717 5991 6138 6549 5319 Size: 398 274 147 411 5656 Fragments arranged by size: 5656 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2042 2072 2192 2222 2892 Size: 30 165 30 639 30 120 30 670 Cuts at: 2892 2922 3344 3374 1178 Size: 30 422 30 4690 Fragments arranged by size: 4690 670 639 422 165 120 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2092 2900 2953 3224 3227 3559 1186 Size: 906 808 53 271 3 332 4513 Fragments arranged by size: 4513 906 808 332 271 53 3 BseYI C'CCAG_C Cuts at: 3341 3737 3751 3775 3893 6089 6585 3341 Size: 396 14 24 118 2196 496 3642 Fragments arranged by size: 3642 2196 496 396 118 24 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2303 2436 3912 4003 1163 Size: 573 567 133 1476 91 4046 Fragments arranged by size: 4046 1476 573 567 133 91 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2313 2451 2524 4336 5097 6125 Size: 275 186 312 138 73 1812 761 1028 Cuts at: 6125 1540 Size: 2301 Fragments arranged by size: 2301 1812 1028 761 312 275 186 138 73 BsiHKAI G_wGCw'C Cuts at: 2057 2297 3093 3519 3580 3997 4169 4947 5032 Size: 240 796 426 61 417 172 778 85 Cuts at: 5032 6103 2057 Size: 1071 2840 Fragments arranged by size: 2840 1071 796 778 426 417 240 172 85 61 BsiWI C'GTAC_G Cuts at: 2058 2058 Size: 6886 BsmI GAATG_Cn' Cuts at: 4397 4490 4397 Size: 93 6793 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2057 2297 2641 2654 3093 3124 3142 Size: 399 267 240 344 13 439 31 18 Cuts at: 3142 3197 3416 3452 3519 3580 3738 3855 3871 Size: 55 219 36 67 61 158 117 16 Cuts at: 3871 3951 3997 4065 4110 4169 4205 4947 5032 Size: 80 46 68 45 59 36 742 85 Cuts at: 5032 6103 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 439 399 344 267 240 219 158 117 85 80 68 67 61 59 55 46 45 36 36 31 18 16 13 BspEI T'CCGG_A Cuts at: 6549 6549 Size: 6886 BspHI T'CATG_A Cuts at: 2793 4775 6505 2793 Size: 1982 1730 3174 Fragments arranged by size: 3174 1982 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4031 4241 844 Size: 3187 210 3489 Fragments arranged by size: 3489 3187 210 BsrBI CCG'CTC Cuts at: 1394 1823 3078 4773 1394 Size: 429 1255 1695 3507 Fragments arranged by size: 3507 1695 1255 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2892 5381 5555 65 Size: 1183 1644 2489 174 1396 Fragments arranged by size: 2489 1644 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2312 2538 5530 5717 1700 Size: 110 502 226 2992 187 2869 Fragments arranged by size: 2992 2869 502 226 187 110 BsrGI T'GTAC_A Cuts at: 96 3834 96 Size: 3738 3148 BssHII G'CGCG_C Cuts at: 1925 2943 1925 Size: 1018 5868 BssSI C'ACGA_G Cuts at: 3592 4946 5958 3592 Size: 1354 1012 4520 Fragments arranged by size: 4520 1354 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2354 3226 3859 3935 794 Size: 1560 872 633 76 3745 Fragments arranged by size: 3745 1560 872 633 76 BstBI TT'CG_AA Cuts at: 2007 5690 2007 Size: 3683 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6886 BstXI CCAn_nnnn'nTGG Cuts at: 3691 3691 Size: 6886 BstYI r'GATC_y Cuts at: 1972 4216 4583 4968 4985 6437 6523 6535 6881 Size: 2244 367 385 17 1452 86 12 346 Cuts at: 6881 1972 Size: 1977 Fragments arranged by size: 2244 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2951 2951 Size: 6886 BtgI C'CryG_G Cuts at: 513 1065 2106 2556 2873 2985 3379 3606 3877 Size: 552 1041 450 317 112 394 227 271 Cuts at: 3877 513 Size: 3522 Fragments arranged by size: 3522 1041 552 450 394 317 271 227 112 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6147 Fragments arranged by size: 6147 683 56 BtsI GCAGTG_nn' Cuts at: 3888 4402 5196 5216 3888 Size: 514 794 20 5558 Fragments arranged by size: 5558 794 514 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6886 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2433 2468 529 Size: 35 1869 35 4947 Fragments arranged by size: 4947 1869 35 35 DraI TTT'AAA Cuts at: 4190 4190 Size: 6886 DraIII CAC_nnn'GTG Cuts at: 3859 3935 3859 Size: 76 6810 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5893 809 Size: 507 4577 1802 Fragments arranged by size: 4577 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2175 2310 3112 Size: 54 1425 50 275 18 345 135 802 Cuts at: 3112 4134 4333 5222 6851 8 Size: 1022 199 889 1629 43 Fragments arranged by size: 1629 1425 1022 889 802 345 275 199 135 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2310 4333 1537 Size: 275 498 2023 4090 Fragments arranged by size: 4090 2023 498 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2258 4816 1353 Size: 905 2558 3423 Fragments arranged by size: 3423 2558 905 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3271 5473 5847 5993 387 Size: 1064 138 1682 2202 374 146 1280 Fragments arranged by size: 2202 1682 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2295 3578 2295 Size: 1283 5603 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2384 3252 3264 3454 3594 3965 4949 Size: 393 752 868 12 190 140 371 984 Cuts at: 4949 5546 6333 6601 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 984 868 787 752 597 393 371 268 190 140 12 EcoNI CCTnn'n_nnAGG Cuts at: 2346 2346 Size: 6886 EcoO109I rG'GnC_Cy Cuts at: 2082 2587 2637 3138 3139 3237 3272 3649 2082 Size: 505 50 501 1 98 35 377 5319 Fragments arranged by size: 5319 505 501 377 98 50 35 1 EcoRI G'AATT_C Cuts at: 4195 4573 4195 Size: 378 6508 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2287 2287 Size: 6886 FspI TGC'GCA Cuts at: 5392 5392 Size: 6886 HaeII r_GCGC'y Cuts at: 1678 2319 2852 6033 1678 Size: 641 533 3181 2531 Fragments arranged by size: 3181 2531 641 533 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2192 2224 2714 2746 3344 Size: 32 135 32 620 32 490 32 598 Cuts at: 3344 3376 3586 3618 5527 5559 5601 5633 1373 Size: 32 210 32 1909 32 42 32 2626 Fragments arranged by size: 2626 1909 620 598 490 210 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4230 4476 669 Size: 988 281 2292 246 3079 Fragments arranged by size: 3079 2292 988 281 246 HindIII A'AGCT_T Cuts at: 748 2222 4155 748 Size: 1474 1933 3479 Fragments arranged by size: 3479 1933 1474 HpaI GTT'AAC Cuts at: 4476 4476 Size: 6886 KasI G'GCGC_C Cuts at: 2315 2848 2315 Size: 533 6353 KpnI G_GTAC'C Cuts at: 4211 4211 Size: 6886 MfeI C'AATT_G Cuts at: 4485 4485 Size: 6886 MluI A'CGCG_T Cuts at: 6762 6762 Size: 6886 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2145 2148 2361 3686 3951 6000 6184 1305 Size: 840 3 213 1325 265 2049 184 2007 Fragments arranged by size: 2049 2007 1325 840 265 213 184 3 MscI TGG'CCA Cuts at: 10 64 1489 1832 2177 4136 10 Size: 54 1425 343 345 1959 2760 Fragments arranged by size: 2760 1959 1425 345 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2513 2798 3148 3689 4078 4844 5203 Size: 648 1347 285 350 541 389 766 359 Cuts at: 5203 5362 518 Size: 159 2042 Fragments arranged by size: 2042 1347 766 648 541 389 359 350 285 159 MspA1I CmG'CkG Cuts at: 1606 2337 2691 2767 2875 2924 2987 3299 3381 Size: 731 354 76 108 49 63 312 82 Cuts at: 3381 4302 4979 6127 6372 1606 Size: 921 677 1148 245 2120 Fragments arranged by size: 2120 1148 921 731 677 354 312 245 108 82 76 63 49 NaeI GCC'GGC Cuts at: 2314 2540 2314 Size: 226 6660 NarI GG'CG_CC Cuts at: 2316 2849 2316 Size: 533 6353 NcoI C'CATG_G Cuts at: 513 1065 2556 3606 3877 513 Size: 552 1491 1050 271 3522 Fragments arranged by size: 3522 1491 1050 552 271 NdeI CA'TA_TG Cuts at: 387 387 Size: 6886 NgoMIV G'CCGG_C Cuts at: 2312 2538 2312 Size: 226 6660 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6886 NotI GC'GGCC_GC Cuts at: 4333 4333 Size: 6886 NsiI A_TGCA'T Cuts at: 2440 2440 Size: 6886 NspI r_CATG'y Cuts at: 1132 1229 4244 5789 1132 Size: 97 3015 1545 2229 Fragments arranged by size: 3015 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6886 PciI A'CATG_T Cuts at: 5785 5785 Size: 6886 PflMI CCAn_nnn'nTGG Cuts at: 1108 4102 1108 Size: 2994 3892 PfoI T'CCnGG_A Cuts at: 3268 3268 Size: 6886 PmeI GTTT'AAAC Cuts at: 4190 4190 Size: 6886 PmlI CAC'GTG Cuts at: 2515 2515 Size: 6886 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4955 4987 6468 6500 4955 Size: 32 1481 32 5341 Fragments arranged by size: 5341 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3272 3649 3272 Size: 377 6509 PshAI GACnn'nnGTC Cuts at: 1613 2337 1613 Size: 724 6162 PsiI TTA'TAA Cuts at: 4456 4456 Size: 6886 PspOMI G'GGCC_C Cuts at: 2637 3138 2637 Size: 501 6385 PstI C_TGCA'G Cuts at: 830 2091 3339 3555 3724 4028 4238 830 Size: 1261 1248 216 169 304 210 3478 Fragments arranged by size: 3478 1261 1248 304 216 210 169 PvuI CG_AT'CG Cuts at: 2001 2451 2001 Size: 450 6436 PvuII CAG'CTG Cuts at: 2337 2767 2337 Size: 430 6456 SacI G_AGCT'C Cuts at: 2297 3580 2297 Size: 1283 5603 SacII CC_GC'GG Cuts at: 2876 2988 3382 2876 Size: 112 394 6380 Fragments arranged by size: 6380 394 112 SalI G'TCGA_C Cuts at: 1936 4228 1936 Size: 2292 4594 SapI GCTCTTCn'nnn_ Cuts at: 2258 2258 Size: 6886 SbfI CC_TGCA'GG Cuts at: 4238 4238 Size: 6886 ScaI AGT'ACT Cuts at: 2672 5134 6846 2672 Size: 2462 1712 2712 Fragments arranged by size: 2712 2462 1712 SexAI A'CCwGG_T Cuts at: 3510 3510 Size: 6886 SfcI C'TryA_G Cuts at: 826 1045 2087 3335 3551 3720 4024 4234 5369 Size: 219 1042 1248 216 169 304 210 1135 Cuts at: 5369 6050 6241 826 Size: 681 191 1471 Fragments arranged by size: 1471 1248 1135 1042 681 304 219 216 210 191 169 SfoI GGC'GCC Cuts at: 2317 2850 2317 Size: 533 6353 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6886 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6886 SmaI CCC'GGG Cuts at: 1872 2747 4111 4213 1872 Size: 875 1364 102 4545 Fragments arranged by size: 4545 1364 875 102 SmlI C'TyrA_G Cuts at: 820 1017 1943 4990 5891 6153 820 Size: 197 926 3047 901 262 1553 Fragments arranged by size: 3047 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4183 4677 493 Size: 3690 494 2702 Fragments arranged by size: 3690 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6886 SphI G_CATG'C Cuts at: 1132 4244 1132 Size: 3112 3774 SspI AAT'ATT Cuts at: 5 52 4810 5 Size: 47 4758 2081 Fragments arranged by size: 4758 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6886 StyI C'CwwG_G Cuts at: 513 1065 2556 2595 3186 3606 3877 4065 4131 Size: 552 1491 39 591 420 271 188 66 Cuts at: 4131 4325 513 Size: 194 3074 Fragments arranged by size: 3074 1491 591 552 420 271 194 188 66 39 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3696 3789 4913 4930 5083 5268 5713 Size: 1255 2116 93 1124 17 153 185 445 Cuts at: 5713 325 Size: 1498 Fragments arranged by size: 2116 1498 1255 1124 445 185 153 93 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2670 3834 5132 Size: 274 80 33 51 1443 693 1164 1298 Cuts at: 5132 6844 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1298 1164 693 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2892 2924 3692 3724 6846 6878 2892 Size: 32 768 32 3122 32 2900 Fragments arranged by size: 3122 2900 768 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3173 1316 Size: 1857 5029 XbaI T'CTAG_A Cuts at: 4222 4222 Size: 6886 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2576 4129 2576 Size: 1553 5333 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6886 XmaI C'CCGG_G Cuts at: 1870 2745 4109 4211 1870 Size: 875 1364 102 4545 Fragments arranged by size: 4545 1364 875 102 XmnI GAAnn'nnTTC Cuts at: 2267 5015 2267 Size: 2748 4138 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4694 276 Size: 53 83 186 936 3160 2468 Fragments arranged by size: 3160 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BsmBI BstZ17I EcoRV FseI FspAI NruI PacI PspXI PsrI RsrII SanDI SfiI SrfI SwaI