Restriction map for
pFN21ASDA1218
##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AhdI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BbeI BbrPI BcgI BciVI
BclI BfrI BglI BlnI BsaAI
BsaBI BsaI BsiEI BsiWI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoRV
EcoT22I FbaI FseI FspI HindIII
KasI KpnI MaeII MamI MfeI
MluI MunI NarI NdeI NheI
NotI NruI NsiI NspI NspV
PflFI PmaCI PmeI PmlI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SnaBI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
XbaI XcmI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccI 293 GTAGAC GT^[AC][GT]AC
AccIII 30 TCCGGA T^CCGGA
AcyI 70 GACGCC G[GA]^CG[CT]C
Alw44I 2014 GTGCAC G^TGCAC
AocI 2063 CCTCAGG CC^T[ACGT]AGG
ApaLI 2014 GTGCAC G^TGCAC
Asp700I 994 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 180 TGGCCA TGG^CCA
BamHI 816 GGATCC G^GATCC
BbvCI 1686 CCTCAGC CCTCAGC(-5/-2)
BglII 683 AGATCT A^GATCT
BlpI 870 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 870 GCTAAGC GC^T[ACGT]AGC
BsaHI 70 GACGCC G[GA]^CG[CT]C
BseAI 30 TCCGGA T^CCGGA
BsgI 219 GTGCAG GTGCAG(16/14)
BsmI 1956 GAATGC GAATGC(1/-1)
BspEI 30 TCCGGA T^CCGGA
BssSI 987 CACGAG CACGAG(-5/-1)
BstEII 1204 GGTGACC G^GT[ACGT]ACC
BstPI 1204 GGTGACC G^GT[ACGT]ACC
BstXI 1298 CCAGACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2063 CCTCAGG CC^T[ACGT]AGG
CelII 870 GCTAAGC GC^T[ACGT]AGC
DraIII 2042 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 2063 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1204 GGTGACC G^GT[ACGT]ACC
EcoT14I 1397 CCATGG C^C[AT][AT]GG
HaeII 1639 AGCGCC [GA]GCGC^[CT]
HgaI 70 GACGC GACGC(5/10)
Hin1I 70 GACGCC G[GA]^CG[CT]C
HincII 2365 GTTAAC GT[CT]^[GA]AC
HindII 2365 GTTAAC GT[CT]^[GA]AC
HpaI 2365 GTTAAC GTT^AAC
KspI 2093 CCGCGG CCGC^GG
MluNI 180 TGGCCA TGG^CCA
MroI 30 TCCGGA T^CCGGA
MscI 180 TGGCCA TGG^CCA
NaeI 1017 GCCGGC GCC^GGC
NcoI 1397 CCATGG C^CATGG
NgoMIV 1017 GCCGGC G^CCGGC
PacI 634 TTAATTAA TTAAT^TAA
PflMI 1192 CCACCGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2180 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 2137 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 584 CTGCAG CTGCA^G
SacI 1050 GAGCTC GAGCT^C
SacII 2093 CCGCGG CCGC^GG
SexAI 233 ACCAGGT A^CC[AT]GGT
SgrAI 2348 CACCGGTG C[GA]^CCGG[CT]G
SmaI 944 CCCGGG CCC^GGG
SpeI 342 ACTAGT A^CTAGT
StyI 1397 CCATGG C^C[AT][AT]GG
Van91I 1192 CCACCGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 944 CCCGGG C^CCGGG
XmaI 944 CCCGGG C^CCGGG
XmnI 994 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccII 2094 CGCG CG^CG
2118 CGCG
AgeI 1194 ACCGGT A^CCGGT
2349 ACCGGT
AspHI 1050 GAGCTC G[AT]GC[AT]^C
2014 GTGCAC
BsiHKAI 1050 GAGCTC G[AT]GC[AT]^C
2014 GTGCAC
BsmBI 618 CGTCTC CGTCTC(1/5)
1916 CGTCTC
BspMI 853 ACCTGC ACCTGC(4/8)
2385 GCAGGT
BstUI 2094 CGCG CG^CG
2118 CGCG
DsaI 1397 CCATGG C^C[GA][CT]GG
2093 CCGCGG
EcoNI 466 CCTGCGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
534 CCTAGTGAAGG
EcoRI 701 GAATTC G^AATTC
1416 GAATTC
FauI 2092 CCCGC CCCGC(4/6)
2331 CCCGC
MspA1I 372 CAGCGG C[AC]G^C[GT]G
2093 CCGCGG
MvnI 2094 CGCG CG^CG
2118 CGCG
PaeR7I 1563 CTCGAG C^TCGAG
2262 CTCGAG
PinAI 1194 ACCGGT A^CCGGT
2349 ACCGGT
SfcI 506 CTGTAG C^T[GA][CT]AG
584 CTGCAG
SmlI 1563 CTCGAG C^T[CT][GA]AG
2262 CTCGAG
XhoI 1563 CTCGAG C^TCGAG
2262 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaJI BsaWI
BseRI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII