(Circular) (Six-base) MAPSORT of: pFN21ASDA1218.seq Check: 6079 from: 1 to: 7228 pFN21ASDA1218 7228 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2885 2885 Size: 7228 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5038 278 Size: 53 83 186 936 3502 2468 Fragments arranged by size: 3502 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2317 4571 1937 Size: 380 2254 4594 Fragments arranged by size: 4594 2254 380 Acc65I G'GTAC_C Cuts at: 4549 4549 Size: 7228 AclI AA'CG_TT Cuts at: 1563 5355 5728 1563 Size: 3792 373 3063 Fragments arranged by size: 3792 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3051 3555 5291 6675 6943 1632 Size: 1419 504 1736 1384 268 1917 Fragments arranged by size: 1917 1736 1419 1384 504 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7031 AflIII A'CryG_T Cuts at: 1203 6127 7104 1203 Size: 4924 977 1327 Fragments arranged by size: 4924 1327 977 AgeI A'CCGG_T Cuts at: 3217 4372 6059 3217 Size: 1155 1687 4386 Fragments arranged by size: 4386 1687 1155 AhdI GACnn_n'nnGTC Cuts at: 5957 5957 Size: 7228 AleI CACnn'nnGTG Cuts at: 1166 3448 1166 Size: 2282 4946 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7228 AlwNI CAG_nnn'CTG Cuts at: 2250 2528 2615 4971 6543 2250 Size: 278 87 2356 1572 2935 Fragments arranged by size: 2935 2356 1572 278 87 ApaLI G'TGCA_C Cuts at: 4037 5285 6441 4037 Size: 1248 1156 4824 Fragments arranged by size: 4824 1248 1156 ApoI r'AATT_y Cuts at: 1493 2547 2724 2807 3439 4537 4763 4915 7024 Size: 1054 177 83 632 1098 226 152 2109 Cuts at: 7024 1493 Size: 1697 Fragments arranged by size: 2109 1697 1098 1054 632 226 177 152 83 AseI AT'TA_AT Cuts at: 160 1032 5782 160 Size: 872 4750 1606 Fragments arranged by size: 4750 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7228 AvaI C'yCGr_G Cuts at: 1870 1943 2843 2967 3586 3658 4285 4553 1870 Size: 73 900 124 619 72 627 268 4545 Fragments arranged by size: 4545 900 627 619 268 124 73 72 BamHI G'GATC_C Cuts at: 2839 4558 4925 2839 Size: 1719 367 5142 Fragments arranged by size: 5142 1719 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2573 2762 4375 4549 6004 Size: 325 217 627 786 189 1613 174 1455 Cuts at: 6004 618 Size: 1842 Fragments arranged by size: 1842 1613 1455 786 627 325 217 189 174 BanII G_rGCy'C Cuts at: 1391 3077 3207 4064 4086 4547 1391 Size: 1686 130 857 22 461 4072 Fragments arranged by size: 4072 1686 857 461 130 22 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2305 3057 3876 4227 928 Size: 971 406 752 819 351 3929 Fragments arranged by size: 3929 971 819 752 406 351 BbvCI CC'TCA_GC Cuts at: 3710 3710 Size: 7228 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5419 5453 1903 Size: 34 3482 34 3678 Fragments arranged by size: 3678 3482 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5120 6330 5120 Size: 1210 6018 BclI T'GATC_A Cuts at: 1548 1548 Size: 7228 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5839 136 Size: 107 122 71 631 605 4167 1525 Fragments arranged by size: 4167 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2706 7223 2706 Size: 4517 2711 BlpI GC'TnA_GC Cuts at: 2894 4645 2894 Size: 1751 5477 Bme1580I G_kGCm'C Cuts at: 1790 2049 4041 4182 4380 5289 6445 1790 Size: 259 1992 141 198 909 1156 2573 Fragments arranged by size: 2573 1992 1156 909 259 198 141 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7228 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2043 3546 4959 Size: 416 53 83 399 24 626 1503 1413 Cuts at: 4959 5917 442 Size: 958 1753 Fragments arranged by size: 1753 1503 1413 958 626 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7228 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3248 3248 Size: 7228 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2316 2325 2446 3183 4100 5888 1239 Size: 1077 9 121 737 917 1788 2579 Fragments arranged by size: 2579 1788 1077 917 737 121 9 Bpu10I CC'TnA_GC Cuts at: 3710 3710 Size: 7228 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5353 6218 6516 5353 Size: 865 298 6065 Fragments arranged by size: 6065 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5891 882 Size: 617 4392 2219 Fragments arranged by size: 4392 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4525 5019 493 Size: 711 3321 494 2702 Fragments arranged by size: 3321 2702 711 494 BsaBI GATnn'nnATC Cuts at: 7222 7222 Size: 7228 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2094 5035 5417 6052 Size: 53 83 186 936 561 2941 382 635 Cuts at: 6052 275 Size: 1451 Fragments arranged by size: 2941 1451 936 635 561 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2053 3217 4372 5661 6059 6333 6480 6891 2053 Size: 1164 1155 1289 398 274 147 411 2390 Fragments arranged by size: 2390 1289 1164 1155 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4382 4412 1178 Size: 30 165 30 2979 30 3994 Fragments arranged by size: 3994 2979 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2611 2930 3886 3889 3892 3895 3898 3901 Size: 1425 319 956 3 3 3 3 3 Cuts at: 3901 3904 4390 1186 Size: 3 486 4024 Fragments arranged by size: 4024 1425 956 486 319 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2212 4358 6431 6927 2212 Size: 2146 2073 496 2513 Fragments arranged by size: 2513 2146 2073 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2263 1163 Size: 573 527 6128 Fragments arranged by size: 6128 573 527 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4678 5439 6467 1540 Size: 275 186 2677 761 1028 2301 Fragments arranged by size: 2677 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3077 4041 5289 5374 6445 3077 Size: 964 1248 85 1071 3860 Fragments arranged by size: 3860 1248 1071 964 85 BsmI GAATG_Cn' Cuts at: 3985 4739 4832 3985 Size: 754 93 6381 Fragments arranged by size: 6381 754 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2647 3945 2647 Size: 1298 5930 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2049 3077 3207 4041 4064 4086 4182 Size: 399 259 1028 130 834 23 22 96 Cuts at: 4182 4380 4547 5289 5374 6445 1391 Size: 198 167 742 85 1071 2174 Fragments arranged by size: 2174 1071 1028 834 742 399 259 198 167 130 96 85 23 22 BspEI T'CCGG_A Cuts at: 2053 6891 2053 Size: 4838 2390 BspHI T'CATG_A Cuts at: 5117 6847 5117 Size: 1730 5498 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2885 4399 4583 844 Size: 2041 1514 184 3489 Fragments arranged by size: 3489 2041 1514 184 BsrBI CCG'CTC Cuts at: 1394 1823 5115 1394 Size: 429 3292 3507 Fragments arranged by size: 3507 3292 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5723 5897 65 Size: 1183 4475 174 1396 Fragments arranged by size: 4475 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3040 3217 4372 5872 6059 1700 Size: 110 1230 177 1155 1500 187 2869 Fragments arranged by size: 2869 1500 1230 1155 187 177 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7228 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7228 BssSI C'ACGA_G Cuts at: 3010 5288 6300 3010 Size: 2278 1012 3938 Fragments arranged by size: 3938 2278 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2573 2615 3104 794 Size: 1779 42 489 4918 Fragments arranged by size: 4918 1779 489 42 BstBI TT'CG_AA Cuts at: 2007 6032 2007 Size: 4025 3203 BstEII G'GTnAC_C Cuts at: 1184 3227 1184 Size: 2043 5185 BstXI CCAn_nnnn'nTGG Cuts at: 3328 3328 Size: 7228 BstYI r'GATC_y Cuts at: 1972 2706 2839 2867 3459 4558 4925 5310 5327 Size: 734 133 28 592 1099 367 385 17 Cuts at: 5327 6779 6865 6877 7223 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1099 734 592 385 367 346 133 86 28 17 12 Bsu36I CC'TnA_GG Cuts at: 4087 4087 Size: 7228 BtgI C'CryG_G Cuts at: 513 1065 2022 3420 4116 513 Size: 552 957 1398 696 3625 Fragments arranged by size: 3625 1398 957 696 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3633 487 Size: 683 56 2407 4082 Fragments arranged by size: 4082 2407 683 56 BtsI GCAGTG_nn' Cuts at: 2107 2251 2287 4162 4744 5538 5558 2107 Size: 144 36 1875 582 794 20 3777 Fragments arranged by size: 3777 1875 794 582 144 36 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7228 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7193 DraI TTT'AAA Cuts at: 4532 4532 Size: 7228 DraIII CAC_nnn'GTG Cuts at: 4070 4070 Size: 7228 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6235 809 Size: 507 4919 1802 Fragments arranged by size: 4919 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2203 2398 3190 Size: 54 1425 50 275 18 373 195 792 Cuts at: 3190 3699 4675 5564 7193 8 Size: 509 976 889 1629 43 Fragments arranged by size: 1629 1425 976 889 792 509 373 275 195 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4675 1537 Size: 275 2863 4090 Fragments arranged by size: 4090 2863 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2127 3604 3922 4521 5158 1353 Size: 774 1477 318 599 637 3423 Fragments arranged by size: 3423 1477 774 637 599 318 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3733 5815 6189 6335 387 Size: 1064 138 2144 2082 374 146 1280 Fragments arranged by size: 2144 2082 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3075 3075 Size: 7228 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2316 2325 2446 3051 3183 3555 4100 Size: 393 684 9 121 605 132 372 545 Cuts at: 4100 5291 5888 6675 6943 1239 Size: 1191 597 787 268 1524 Fragments arranged by size: 1524 1191 787 684 605 597 545 393 372 268 132 121 9 EcoNI CCTnn'n_nnAGG Cuts at: 2493 2561 2493 Size: 68 7160 EcoO109I rG'GnC_Cy Cuts at: 3736 3953 4204 3736 Size: 217 251 6760 Fragments arranged by size: 6760 251 217 EcoRI G'AATT_C Cuts at: 2724 3439 4537 4915 2724 Size: 715 1098 378 5037 Fragments arranged by size: 5037 1098 715 378 FspI TGC'GCA Cuts at: 5734 5734 Size: 7228 HaeII r_GCGC'y Cuts at: 1678 3666 6375 1678 Size: 1988 2709 2531 Fragments arranged by size: 2709 2531 1988 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2020 2052 4498 4530 5869 Size: 32 135 32 448 32 2446 32 1339 Cuts at: 5869 5901 5943 5975 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2446 1339 448 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4390 4572 4818 669 Size: 988 281 2452 182 246 3079 Fragments arranged by size: 3079 2452 988 281 246 182 HindIII A'AGCT_T Cuts at: 748 748 Size: 7228 HpaI GTT'AAC Cuts at: 4390 4818 4390 Size: 428 6800 KpnI G_GTAC'C Cuts at: 4553 4553 Size: 7228 MfeI C'AATT_G Cuts at: 4827 4827 Size: 7228 MluI A'CGCG_T Cuts at: 7104 7104 Size: 7228 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2139 3202 6342 6526 1305 Size: 834 1063 3140 184 2007 Fragments arranged by size: 3140 2007 1063 834 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2205 10 Size: 54 1425 343 373 5033 Fragments arranged by size: 5033 1425 373 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2757 3448 4319 5186 5545 5704 518 Size: 648 1591 691 871 867 359 159 2042 Fragments arranged by size: 2042 1591 871 867 691 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2397 4118 4644 5321 6469 6714 1606 Size: 791 1721 526 677 1148 245 2120 Fragments arranged by size: 2120 1721 1148 791 677 526 245 NaeI GCC'GGC Cuts at: 3042 3042 Size: 7228 NcoI C'CATG_G Cuts at: 513 1065 2022 3420 513 Size: 552 957 1398 4321 Fragments arranged by size: 4321 1398 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7228 NgoMIV G'CCGG_C Cuts at: 3040 3040 Size: 7228 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7228 NotI GC'GGCC_GC Cuts at: 4675 4675 Size: 7228 NspI r_CATG'y Cuts at: 1132 1229 4586 6131 1132 Size: 97 3357 1545 2229 Fragments arranged by size: 3357 2229 1545 97 PacI TTA_AT'TAA Cuts at: 2661 2661 Size: 7228 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7228 PciI A'CATG_T Cuts at: 6127 6127 Size: 7228 PflMI CCAn_nnn'nTGG Cuts at: 1108 3221 1108 Size: 2113 5115 PfoI T'CCnGG_A Cuts at: 2084 2084 Size: 7228 PmeI GTTT'AAAC Cuts at: 4532 4532 Size: 7228 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5297 5329 6810 6842 5297 Size: 32 1481 32 5683 Fragments arranged by size: 5683 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4204 4204 Size: 7228 PshAI GACnn'nnGTC Cuts at: 1613 4164 1613 Size: 2551 4677 PsiI TTA'TAA Cuts at: 4798 4798 Size: 7228 PspXI vC'TCGA_Gb Cuts at: 3586 4285 3586 Size: 699 6529 PstI C_TGCA'G Cuts at: 830 2611 4580 830 Size: 1781 1969 3478 Fragments arranged by size: 3478 1969 1781 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7228 SacI G_AGCT'C Cuts at: 3077 3077 Size: 7228 SacII CC_GC'GG Cuts at: 4119 4119 Size: 7228 SalI G'TCGA_C Cuts at: 1936 4570 1936 Size: 2634 4594 SbfI CC_TGCA'GG Cuts at: 4580 4580 Size: 7228 ScaI AGT'ACT Cuts at: 5476 7188 5476 Size: 1712 5516 SexAI A'CCwGG_T Cuts at: 2256 2256 Size: 7228 SfcI C'TryA_G Cuts at: 826 1045 2529 2607 4576 5711 6392 6583 826 Size: 219 1484 78 1969 1135 681 191 1471 Fragments arranged by size: 1969 1484 1471 1135 681 219 191 78 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7228 SgrAI Cr'CCGG_yG Cuts at: 1700 4372 1700 Size: 2672 4556 SmaI CCC'GGG Cuts at: 1872 2969 4555 1872 Size: 1097 1586 4545 Fragments arranged by size: 4545 1586 1097 SmlI C'TyrA_G Cuts at: 820 1017 1943 3586 4285 5332 6233 6495 820 Size: 197 926 1643 699 1047 901 262 1553 Fragments arranged by size: 1643 1553 1047 926 901 699 262 197 SnaBI TAC'GTA Cuts at: 493 4525 5019 493 Size: 4032 494 2702 Fragments arranged by size: 4032 2702 494 SpeI A'CTAG_T Cuts at: 152 2365 152 Size: 2213 5015 SphI G_CATG'C Cuts at: 1132 4586 1132 Size: 3454 3774 SspI AAT'ATT Cuts at: 5 52 5152 5 Size: 47 5100 2081 Fragments arranged by size: 5100 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7228 StyI C'CwwG_G Cuts at: 513 1065 2022 3420 4667 513 Size: 552 957 1398 1247 3074 Fragments arranged by size: 3074 1398 1247 957 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3136 5255 5272 5425 5610 6055 325 Size: 1255 1556 2119 17 153 185 445 1498 Fragments arranged by size: 2119 1556 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2368 3130 5474 Size: 274 80 33 51 1443 391 762 2344 Cuts at: 5474 7186 96 Size: 1712 138 Fragments arranged by size: 2344 1712 1443 762 391 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2262 2294 2470 2502 3502 3534 3776 3808 7188 Size: 32 176 32 1000 32 242 32 3380 Cuts at: 7188 7220 2262 Size: 32 2270 Fragments arranged by size: 3380 2270 1000 242 176 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7228 XbaI T'CTAG_A Cuts at: 4564 4564 Size: 7228 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2029 2029 Size: 7228 XhoI C'TCGA_G Cuts at: 1943 3586 4285 1943 Size: 1643 699 4886 Fragments arranged by size: 4886 1643 699 XmaI C'CCGG_G Cuts at: 1870 2967 4553 1870 Size: 1097 1586 4545 Fragments arranged by size: 4545 1586 1097 XmnI GAAnn'nnTTC Cuts at: 3021 5357 3021 Size: 2336 4892 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5036 276 Size: 53 83 186 936 3502 2468 Fragments arranged by size: 3502 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI SacI SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PmlI PspOMI PsrI PvuII RsrII SanDI SapI SfiI SfoI SrfI SwaI