Restriction map for
pFN21ASDA1218
(Linear) (Six-base) MAPSORT of: KIAA1218.seq Check: 6300 from: 1 to: 2499
KIAA1218.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 862 2499
Size: 862 1637
AccI GT'mk_AC
Cuts at: 0 294 2499
Size: 294 2205
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1028 1532 2499
Size: 1028 504 967
Fragments arranged by size:
1028 967 504
AgeI A'CCGG_T
Cuts at: 0 1194 2349 2499
Size: 1194 1155 150
Fragments arranged by size:
1194 1155 150
AleI CACnn'nnGTG
Cuts at: 0 1425 2499
Size: 1425 1074
AlwNI CAG_nnn'CTG
Cuts at: 0 227 505 592 2499
Size: 227 278 87 1907
Fragments arranged by size:
1907 278 227 87
ApaLI G'TGCA_C
Cuts at: 0 2014 2499
Size: 2014 485
ApoI r'AATT_y
Cuts at: 0 524 701 784 1416 2499
Size: 524 177 83 632 1083
Fragments arranged by size:
1083 632 524 177 83
AvaI C'yCGr_G
Cuts at: 0 820 944 1563 1635 2262 2499
Size: 820 124 619 72 627 237
Fragments arranged by size:
820 627 619 237 124 72
BamHI G'GATC_C
Cuts at: 0 816 2499
Size: 816 1683
BanI G'GyrC_C
Cuts at: 0 550 739 2352 2499
Size: 550 189 1613 147
Fragments arranged by size:
1613 550 189 147
BanII G_rGCy'C
Cuts at: 0 1054 1184 2041 2063 2499
Size: 1054 130 857 22 436
Fragments arranged by size:
1054 857 436 130 22
BbsI GAAGACnn'nnnn_
Cuts at: 0 282 1034 1853 2204 2499
Size: 282 752 819 351 295
Fragments arranged by size:
819 752 351 295 282
BbvCI CC'TCA_GC
Cuts at: 0 1687 2499
Size: 1687 812
BglII A'GATC_T
Cuts at: 0 683 2499
Size: 683 1816
BlpI GC'TnA_GC
Cuts at: 0 871 2499
Size: 871 1628
Bme1580I G_kGCm'C
Cuts at: 0 26 2018 2159 2357 2499
Size: 26 1992 141 198 142
Fragments arranged by size:
1992 198 142 141 26
BmrI ACTGGGnnnn_n'
Cuts at: 0 20 1523 2499
Size: 20 1503 976
Fragments arranged by size:
1503 976 20
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1225 2499
Size: 1225 1274
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 302 423 1160 2077 2499
Size: 293 9 121 737 917 422
Fragments arranged by size:
917 737 422 293 121 9
Bpu10I CC'TnA_GC
Cuts at: 0 1687 2499
Size: 1687 812
BsaHI Gr'CG_yC
Cuts at: 0 71 2499
Size: 71 2428
BsaWI w'CCGG_w
Cuts at: 0 30 1194 2349 2499
Size: 30 1164 1155 150
Fragments arranged by size:
1164 1155 150 30
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2359 2389 2499
Size: 2359 30 110
Fragments arranged by size:
2359 110 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 588 907 1863 1866 1869 1872 1875 1878
Size: 588 319 956 3 3 3 3 3
Cuts at: 1878 1881 2367 2499
Size: 3 486 132
Fragments arranged by size:
956 588 486 319 132 3 3 3
3 3 3
BseYI C'CCAG_C
Cuts at: 0 189 2335 2499
Size: 189 2146 164
Fragments arranged by size:
2146 189 164
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 240 2499
Size: 240 2259
BsiHKAI G_wGCw'C
Cuts at: 0 1054 2018 2499
Size: 1054 964 481
Fragments arranged by size:
1054 964 481
BsmI GAATG_Cn'
Cuts at: 0 1962 2499
Size: 1962 537
BsmBI CGTCTCn'nnnn_
Cuts at: 0 624 1922 2499
Size: 624 1298 577
Fragments arranged by size:
1298 624 577
Bsp1286I G_dGCh'C
Cuts at: 0 26 1054 1184 2018 2041 2063 2159 2357
Size: 26 1028 130 834 23 22 96 198
Cuts at: 2357 2499
Size: 142
Fragments arranged by size:
1028 834 198 142 130 96 26 23
22
BspEI T'CCGG_A
Cuts at: 0 30 2499
Size: 30 2469
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 862 2376 2499
Size: 862 1514 123
Fragments arranged by size:
1514 862 123
BsrFI r'CCGG_y
Cuts at: 0 1017 1194 2349 2499
Size: 1017 177 1155 150
Fragments arranged by size:
1155 1017 177 150
BssSI C'ACGA_G
Cuts at: 0 987 2499
Size: 987 1512
BstAPI GCAn_nnn'nTGC
Cuts at: 0 550 592 1081 2499
Size: 550 42 489 1418
Fragments arranged by size:
1418 550 489 42
BstEII G'GTnAC_C
Cuts at: 0 1204 2499
Size: 1204 1295
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1305 2499
Size: 1305 1194
BstYI r'GATC_y
Cuts at: 0 683 816 844 1436 2499
Size: 683 133 28 592 1063
Fragments arranged by size:
1063 683 592 133 28
Bsu36I CC'TnA_GG
Cuts at: 0 2064 2499
Size: 2064 435
BtgI C'CryG_G
Cuts at: 0 1397 2093 2499
Size: 1397 696 406
Fragments arranged by size:
1397 696 406
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1610 2499
Size: 1610 889
BtsI GCAGTG_nn'
Cuts at: 0 84 228 264 2139 2499
Size: 84 144 36 1875 360
Fragments arranged by size:
1875 360 144 84 36
DraIII CAC_nnn'GTG
Cuts at: 0 2047 2499
Size: 2047 452
EaeI y'GGCC_r
Cuts at: 0 180 375 1167 1676 2499
Size: 180 195 792 509 823
Fragments arranged by size:
823 792 509 195 180
EarI CTCTTCn'nnn_
Cuts at: 0 104 1581 1899 2498 2499
Size: 104 1477 318 599 1
Fragments arranged by size:
1477 599 318 104 1
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1710 2499
Size: 1710 789
EcoICRI GAG'CTC
Cuts at: 0 1052 2499
Size: 1052 1447
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 302 423 1028 1160 1532 2077 2499
Size: 293 9 121 605 132 372 545 422
Fragments arranged by size:
605 545 422 372 293 132 121 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 470 538 2499
Size: 470 68 1961
Fragments arranged by size:
1961 470 68
EcoO109I rG'GnC_Cy
Cuts at: 0 1713 1930 2181 2499
Size: 1713 217 251 318
Fragments arranged by size:
1713 318 251 217
EcoRI G'AATT_C
Cuts at: 0 701 1416 2499
Size: 701 715 1083
Fragments arranged by size:
1083 715 701
HaeII r_GCGC'y
Cuts at: 0 1643 2499
Size: 1643 856
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 29 2475 2499
Size: 29 2446 24
Fragments arranged by size:
2446 29 24
HincII GTy'rAC
Cuts at: 0 2367 2499
Size: 2367 132
HpaI GTT'AAC
Cuts at: 0 2367 2499
Size: 2367 132
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 1179 2499
Size: 116 1063 1320
Fragments arranged by size:
1320 1063 116
MscI TGG'CCA
Cuts at: 0 182 2499
Size: 182 2317
MslI CAynn'nnrTG
Cuts at: 0 734 1425 2296 2499
Size: 734 691 871 203
Fragments arranged by size:
871 734 691 203
MspA1I CmG'CkG
Cuts at: 0 374 2095 2499
Size: 374 1721 404
Fragments arranged by size:
1721 404 374
NaeI GCC'GGC
Cuts at: 0 1019 2499
Size: 1019 1480
NcoI C'CATG_G
Cuts at: 0 1397 2499
Size: 1397 1102
NgoMIV G'CCGG_C
Cuts at: 0 1017 2499
Size: 1017 1482
PacI TTA_AT'TAA
Cuts at: 0 638 2499
Size: 638 1861
PflMI CCAn_nnn'nTGG
Cuts at: 0 1198 2499
Size: 1198 1301
PfoI T'CCnGG_A
Cuts at: 0 61 2499
Size: 61 2438
PpuMI rG'GwC_Cy
Cuts at: 0 2181 2499
Size: 2181 318
PshAI GACnn'nnGTC
Cuts at: 0 2141 2499
Size: 2141 358
PspXI vC'TCGA_Gb
Cuts at: 0 1563 2262 2499
Size: 1563 699 237
Fragments arranged by size:
1563 699 237
PstI C_TGCA'G
Cuts at: 0 588 2499
Size: 588 1911
SacI G_AGCT'C
Cuts at: 0 1054 2499
Size: 1054 1445
SacII CC_GC'GG
Cuts at: 0 2096 2499
Size: 2096 403
SexAI A'CCwGG_T
Cuts at: 0 233 2499
Size: 233 2266
SfcI C'TryA_G
Cuts at: 0 506 584 2499
Size: 506 78 1915
Fragments arranged by size:
1915 506 78
SgrAI Cr'CCGG_yG
Cuts at: 0 2349 2499
Size: 2349 150
SmaI CCC'GGG
Cuts at: 0 946 2499
Size: 946 1553
SmlI C'TyrA_G
Cuts at: 0 1563 2262 2499
Size: 1563 699 237
Fragments arranged by size:
1563 699 237
SpeI A'CTAG_T
Cuts at: 0 342 2499
Size: 342 2157
StyI C'CwwG_G
Cuts at: 0 1397 2499
Size: 1397 1102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1113 2499
Size: 1113 1386
TatI w'GTAC_w
Cuts at: 0 345 1107 2499
Size: 345 762 1392
Fragments arranged by size:
1392 762 345
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 239 271 447 479 1479 1511 1753 1785
Size: 239 32 176 32 1000 32 242 32
Cuts at: 1785 2499
Size: 714
Fragments arranged by size:
1000 714 242 239 176 32 32 32
32
XhoI C'TCGA_G
Cuts at: 0 1563 2262 2499
Size: 1563 699 237
Fragments arranged by size:
1563 699 237
XmaI C'CCGG_G
Cuts at: 0 944 2499
Size: 944 1555
XmnI GAAnn'nnTTC
Cuts at: 0 998 2499
Size: 998 1501
Enzymes that do cut:
AarI AccI AcuI AgeI AleI AlwNI ApaLI ApoI
AvaI BamHI BanI BanII BbsI BbvCI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspMI BsrFI BssSI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraIII EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I HincII HpaI
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV PacI
PflMI PfoI PpuMI PshAI PspXI PstI SacI SacII
SexAI SfcI SgrAI SmaI SmlI SpeI StyI TaqII
TatI TstI XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AhdI AlfI
AloI ApaI AscI AseI AsiSI AvrII BaeI BbeI
BcgI BciVI BclI BfrBI BglI BmgBI BmtI BpuEI
BsaI BsaAI BsaBI BsiEI BsiWI BspHI BsrBI BsrDI
BsrGI BssHII BstBI BstZ17I ClaI CspCI DraI DrdI
EagI EcoRV FalI FseI FspI FspAI HindIII KasI
KpnI MfeI MluI NarI NdeI NheI NotI NruI
NsiI NspI PasI PciI PmeI PmlI PpiI PsiI
PspOMI PsrI PvuI PvuII RsrII SalI SanDI SapI
SbfI ScaI SfiI SfoI SgfI SnaBI SphI SrfI
SspI StuI SwaI Tth111I XbaI XcmI ZraI