Restriction map for
pFN21ASDA1217
(Circular) (Six-base) MAPSORT of: pFN21ASDA1217.seq Check: 7812 from: 1 to: 8521
pFN21ASDA1217 8521 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5479 5533 5479
Size: 54 8467
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2213 4221 6331 278
Size: 53 83 186 936 677 2008 2110 2468
Fragments arranged by size:
2468 2110 2008 936 677 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2729 3384 4995 5864 1937
Size: 792 655 1611 869 4594
Fragments arranged by size:
4594 1611 869 792 655
Acc65I G'GTAC_C
Cuts at: 5842 5842
Size: 8521
AclI AA'CG_TT
Cuts at: 1563 6648 7021 1563
Size: 5085 373 3063
Fragments arranged by size:
5085 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2093 4181 4214 4349 5082 6584 7968 8236
Size: 461 2088 33 135 733 1502 1384 268
Cuts at: 8236 1632
Size: 1917
Fragments arranged by size:
2088 1917 1502 1384 733 461 268 135
33
AfeI AGC'GCT
Cuts at: 3361 3361
Size: 8521
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8324
AflIII A'CryG_T
Cuts at: 1203 4240 7420 8397 1203
Size: 3037 3180 977 1327
Fragments arranged by size:
3180 3037 1327 977
AgeI A'CCGG_T
Cuts at: 7352 7352
Size: 8521
AhdI GACnn_n'nnGTC
Cuts at: 3893 7250 3893
Size: 3357 5164
AleI CACnn'nnGTG
Cuts at: 1166 3335 1166
Size: 2169 6352
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3435 5444 1184
Size: 2251 2009 4261
Fragments arranged by size:
4261 2251 2009
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3738 3770 5407 5439 3738
Size: 32 1637 32 6820
Fragments arranged by size:
6820 1637 32 32
AlwNI CAG_nnn'CTG
Cuts at: 6264 7836 6264
Size: 1572 6949
ApaI G_GGCC'C
Cuts at: 2674 3537 5212 2674
Size: 863 1675 5983
Fragments arranged by size:
5983 1675 863
ApaLI G'TGCA_C
Cuts at: 6578 7734 6578
Size: 1156 7365
ApoI r'AATT_y
Cuts at: 1493 2716 2769 4094 4876 5830 6056 6208 8317
Size: 1223 53 1325 782 954 226 152 2109
Cuts at: 8317 1493
Size: 1697
Fragments arranged by size:
2109 1697 1325 1223 954 782 226 152
53
AseI AT'TA_AT
Cuts at: 160 1032 7075 160
Size: 872 6043 1606
Fragments arranged by size:
6043 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8521
AvaI C'yCGr_G
Cuts at: 1870 1943 5846 1870
Size: 73 3903 4545
Fragments arranged by size:
4545 3903 73
BamHI G'GATC_C
Cuts at: 2575 3874 5299 5851 6218 2575
Size: 1299 1425 552 367 4878
Fragments arranged by size:
4878 1425 1299 552 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2783 3329 3667 3695 4382
Size: 325 217 627 996 546 338 28 687
Cuts at: 4382 4411 5711 5720 5842 7297 618
Size: 29 1300 9 122 1455 1842
Fragments arranged by size:
1842 1455 1300 996 687 627 546 338
325 217 122 29 28 9
BanII G_rGCy'C
Cuts at: 1391 2147 2674 3185 3537 4257 4451 5201 5212
Size: 756 527 511 352 720 194 750 11
Cuts at: 5212 5840 1391
Size: 628 4072
Fragments arranged by size:
4072 756 750 720 628 527 511 352
194 11
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2919 3975 3987 4044 928
Size: 971 1020 1056 12 57 5405
Fragments arranged by size:
5405 1056 1020 971 57 12
BbvCI CC'TCA_GC
Cuts at: 3700 3803 3700
Size: 103 8418
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5209 5243 6712 6746 1903
Size: 34 3272 34 1469 34 3678
Fragments arranged by size:
3678 3272 1469 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6413 7623 6413
Size: 1210 7311
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8521
BfrBI ATG'CAT
Cuts at: 3115 3115
Size: 8521
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7132 136
Size: 107 122 71 631 605 5460 1525
Fragments arranged by size:
5460 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2327 8516 2327
Size: 6189 2332
BlpI GC'TnA_GC
Cuts at: 5938 5938
Size: 8521
Bme1580I G_kGCm'C
Cuts at: 1790 2674 2742 2786 3537 3698 4387 5212 5723
Size: 884 68 44 751 161 689 825 511
Cuts at: 5723 6582 7738 1790
Size: 859 1156 2573
Fragments arranged by size:
2573 1156 884 859 825 751 689 511
161 68 44
BmgBI CAC'GTC
Cuts at: 1318 4033 1318
Size: 2715 5806
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2169 2466 3188
Size: 416 53 83 399 24 752 297 722
Cuts at: 3188 4835 6252 7210 442
Size: 1647 1417 958 1753
Fragments arranged by size:
1753 1647 1417 958 752 722 416 399
297 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8521
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5604 5604
Size: 8521
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2333 2718 2821 2836 3677 3740 3746 3764
Size: 1094 385 103 15 841 63 6 18
Cuts at: 3764 3890 4817 5177 5185 7181 1239
Size: 126 927 360 8 1996 2579
Fragments arranged by size:
2579 1996 1094 927 841 385 360 126
103 63 18 15 8 6
Bpu10I CC'TnA_GC
Cuts at: 2375 3700 3803 4764 5025 2375
Size: 1325 103 961 261 5871
Fragments arranged by size:
5871 1325 961 261 103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3743 6646 7511 7809 3743
Size: 2903 865 298 4455
Fragments arranged by size:
4455 2903 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2124 3449 7184 882
Size: 617 625 1325 3735 2219
Fragments arranged by size:
3735 2219 1325 625 617
BsaAI yAC'GTr
Cuts at: 493 1204 4519 5818 6312 493
Size: 711 3315 1299 494 2702
Fragments arranged by size:
3315 2702 1299 711 494
BsaBI GATnn'nnATC
Cuts at: 8515 8515
Size: 8521
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2210 4218 6328 6710
Size: 53 83 186 936 677 2008 2110 382
Cuts at: 6710 7345 275
Size: 635 1451
Fragments arranged by size:
2110 2008 1451 936 677 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3069 3157 6954 7352 7626 7773 8184 3069
Size: 88 3797 398 274 147 411 3406
Fragments arranged by size:
3797 3406 411 398 274 147 88
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2819 2849 3655 3685 3738
Size: 30 165 30 1416 30 806 30 53
Cuts at: 3738 3768 4059 4089 5522 5552 1178
Size: 30 291 30 1433 30 4147
Fragments arranged by size:
4147 1433 1416 806 291 165 53 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2546 2670 3195 3387 4181 4355 4911 4914
Size: 1360 124 525 192 794 174 556 3
Cuts at: 4914 4926 5102 5125 5128 5165 5659 5680 1186
Size: 12 176 23 3 37 494 21 4027
Fragments arranged by size:
4027 1360 794 556 525 494 192 176
174 124 37 23 21 12 3 3
BseYI C'CCAG_C
Cuts at: 3674 4473 4531 4900 5405 5741 7724 8220 3674
Size: 799 58 369 505 336 1983 496 3975
Fragments arranged by size:
3975 1983 799 505 496 369 336 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2421 2565 3633 3902 1163
Size: 573 685 144 1068 269 5782
Fragments arranged by size:
5782 1068 685 573 269 144
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2193 5971 6732 7760 1540
Size: 275 186 192 3778 761 1028 2301
Fragments arranged by size:
3778 2301 1028 761 275 192 186
BsiHKAI G_wGCw'C
Cuts at: 2419 3655 4451 5647 6582 6667 7738 2419
Size: 1236 796 1196 935 85 1071 3202
Fragments arranged by size:
3202 1236 1196 1071 935 796 85
BsmI GAATG_Cn'
Cuts at: 6032 6125 6032
Size: 93 8428
BsmBI CGTCTCn'nnnn_
Cuts at: 2201 5701 2201
Size: 3500 5021
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2147 2419 2674 2742 2786 3185 3537
Size: 399 357 272 255 68 44 399 352
Cuts at: 3537 3655 3698 4257 4387 4451 5201 5212 5647
Size: 118 43 559 130 64 750 11 435
Cuts at: 5647 5723 5840 6582 6667 7738 1391
Size: 76 117 742 85 1071 2174
Fragments arranged by size:
2174 1071 750 742 559 435 399 399
357 352 272 255 130 118 117 85
76 68 64 44 43 11
BspEI T'CCGG_A
Cuts at: 3157 8184 3157
Size: 5027 3494
BspHI T'CATG_A
Cuts at: 3928 4776 6410 8140 3928
Size: 848 1634 1730 4309
Fragments arranged by size:
4309 1730 1634 848
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4959 5386 5479 5533 5876 844
Size: 4115 427 93 54 343 3489
Fragments arranged by size:
4115 3489 427 343 93 54
BsrBI CCG'CTC
Cuts at: 1394 1823 2790 6408 1394
Size: 429 967 3618 3507
Fragments arranged by size:
3618 3507 967 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2815 2931 2943 3045 3816 7016 7190
Size: 1183 1567 116 12 102 771 3200 174
Cuts at: 7190 65
Size: 1396
Fragments arranged by size:
3200 1567 1396 1183 771 174 116 102
12
BsrFI r'CCGG_y
Cuts at: 1700 1810 5000 7165 7352 1700
Size: 110 3190 2165 187 2869
Fragments arranged by size:
3190 2869 2165 187 110
BsrGI T'GTAC_A
Cuts at: 96 2565 2940 3132 96
Size: 2469 375 192 5485
Fragments arranged by size:
5485 2469 375 192
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8521
BssSI C'ACGA_G
Cuts at: 2280 2981 4975 6581 7593 2280
Size: 701 1994 1606 1012 3208
Fragments arranged by size:
3208 1994 1606 1012 701
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8521
BstBI TT'CG_AA
Cuts at: 2007 7325 2007
Size: 5318 3203
BstEII G'GTnAC_C
Cuts at: 1184 2111 2183 5603 1184
Size: 927 72 3420 4102
Fragments arranged by size:
4102 3420 927 72
BstXI CCAn_nnnn'nTGG
Cuts at: 3632 4015 3632
Size: 383 8138
BstYI r'GATC_y
Cuts at: 1972 2327 2575 2684 3874 5299 5851 6218 6603
Size: 355 248 109 1190 1425 552 367 385
Cuts at: 6603 6620 8072 8158 8170 8516 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1425 1190 552 385 367 355
346 248 109 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3224 3224
Size: 8521
BtgI C'CryG_G
Cuts at: 513 1065 2793 3010 3259 3353 3477 3663 3999
Size: 552 1728 217 249 94 124 186 336
Cuts at: 3999 4482 513
Size: 483 4552
Fragments arranged by size:
4552 1728 552 483 336 249 217 186
124 94
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2373 4407 5001 487
Size: 683 56 1147 2034 594 4007
Fragments arranged by size:
4007 2034 1147 683 594 56
BtsI GCAGTG_nn'
Cuts at: 4674 4820 5041 6037 6831 6851 4674
Size: 146 221 996 794 20 6344
Fragments arranged by size:
6344 996 794 221 146 20
ClaI AT'CG_AT
Cuts at: 1554 5486 1554
Size: 3932 4589
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8486
DraI TTT'AAA
Cuts at: 3316 3529 5825 3316
Size: 213 2296 6012
Fragments arranged by size:
6012 2296 213
DraIII CAC_nnn'GTG
Cuts at: 4483 4648 4483
Size: 165 8356
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7528 809
Size: 507 6212 1802
Fragments arranged by size:
6212 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4360 4521 5638
Size: 54 1425 50 275 18 2530 161 1117
Cuts at: 5638 5968 6857 8486 8
Size: 330 889 1629 43
Fragments arranged by size:
2530 1629 1425 1117 889 330 275 161
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5968 1537
Size: 275 4156 4090
Fragments arranged by size:
4156 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2042 2426 2887 3857 3930 4325 5440 6451
Size: 689 384 461 970 73 395 1115 1011
Cuts at: 6451 1353
Size: 3423
Fragments arranged by size:
3423 1115 1011 970 689 461 395 384
73
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3748 3758 4917 7108 7482 7628
Size: 1064 138 2159 10 1159 2191 374 146
Cuts at: 7628 387
Size: 1280
Fragments arranged by size:
2191 2159 1280 1159 1064 374 146 138
10
EcoICRI GAG'CTC
Cuts at: 4449 4449
Size: 8521
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2093 2333 2718 2821 2836 3677 3740
Size: 393 461 240 385 103 15 841 63
Cuts at: 3740 3746 3764 3890 4181 4214 4349 4817 5082
Size: 6 18 126 291 33 135 468 265
Cuts at: 5082 5177 5185 6584 7181 7968 8236 1239
Size: 95 8 1399 597 787 268 1524
Fragments arranged by size:
1524 1399 841 787 597 468 461 393
385 291 268 265 240 135 126 103
95 63 33 18 15 8 6
EcoO109I rG'GnC_Cy
Cuts at: 2670 2671 3533 5208 5209 2670
Size: 1 862 1675 1 5982
Fragments arranged by size:
5982 1675 862 1 1
EcoRI G'AATT_C
Cuts at: 2769 5830 6208 2769
Size: 3061 378 5082
Fragments arranged by size:
5082 3061 378
FspI TGC'GCA
Cuts at: 4133 4205 7027 4133
Size: 72 2822 5627
Fragments arranged by size:
5627 2822 72
HaeII r_GCGC'y
Cuts at: 1678 2880 3363 7668 1678
Size: 1202 483 4305 2531
Fragments arranged by size:
4305 2531 1202 483
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2186 2218 2541 2573 2819
Size: 32 135 32 614 32 323 32 246
Cuts at: 2819 2851 3278 3310 3879 3911 4385 4417 4950
Size: 32 427 32 569 32 474 32 533
Cuts at: 4950 4982 7162 7194 7236 7268 1373
Size: 32 2180 32 42 32 2626
Fragments arranged by size:
2626 2180 614 569 533 474 427 323
246 135 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2307 5865 6111 669
Size: 988 281 369 3558 246 3079
Fragments arranged by size:
3558 3079 988 369 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8521
HpaI GTT'AAC
Cuts at: 6111 6111
Size: 8521
KpnI G_GTAC'C
Cuts at: 5846 5846
Size: 8521
MfeI C'AATT_G
Cuts at: 6120 6120
Size: 8521
MluI A'CGCG_T
Cuts at: 8397 8397
Size: 8521
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2276 3945 4124 4383 7635 7819 1305
Size: 971 1669 179 259 3252 184 2007
Fragments arranged by size:
3252 2007 1669 971 259 184 179
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4523 10
Size: 54 1425 343 2691 4008
Fragments arranged by size:
4008 2691 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2456 2627 2936 3252 3335 3710 4139
Size: 648 1290 171 309 316 83 375 429
Cuts at: 4139 4298 4728 6479 6838 6997 518
Size: 159 430 1751 359 159 2042
Fragments arranged by size:
2042 1751 1290 648 430 429 375 359
316 309 171 159 159 83
MspA1I CmG'CkG
Cuts at: 1606 2216 3784 5205 5937 6614 7762 8007 1606
Size: 610 1568 1421 732 677 1148 245 2120
Fragments arranged by size:
2120 1568 1421 1148 732 677 610 245
NcoI C'CATG_G
Cuts at: 513 1065 2793 3010 3477 513
Size: 552 1728 217 467 5557
Fragments arranged by size:
5557 1728 552 467 217
NdeI CA'TA_TG
Cuts at: 387 3117 387
Size: 2730 5791
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8521
NotI GC'GGCC_GC
Cuts at: 5968 5968
Size: 8521
NsiI A_TGCA'T
Cuts at: 3117 3117
Size: 8521
NspI r_CATG'y
Cuts at: 1132 1229 2588 3241 4244 5879 7424 1132
Size: 97 1359 653 1003 1635 1545 2229
Fragments arranged by size:
2229 1635 1545 1359 1003 653 97
PasI CC'CwG_GG
Cuts at: 1348 2666 2823 1348
Size: 1318 157 7046
Fragments arranged by size:
7046 1318 157
PciI A'CATG_T
Cuts at: 4240 7420 4240
Size: 3180 5341
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8521
PmeI GTTT'AAAC
Cuts at: 5825 5825
Size: 8521
PmlI CAC'GTG
Cuts at: 4519 4519
Size: 8521
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3738 3770 6590 6622 8103 8135 3738
Size: 32 2820 32 1481 32 4124
Fragments arranged by size:
4124 2820 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 4217 1613
Size: 2604 5917
PsiI TTA'TAA
Cuts at: 6091 6091
Size: 8521
PspOMI G'GGCC_C
Cuts at: 2670 3533 5208 2670
Size: 863 1675 5983
Fragments arranged by size:
5983 1675 863
PstI C_TGCA'G
Cuts at: 830 3367 3789 4566 5298 5873 830
Size: 2537 422 777 732 575 3478
Fragments arranged by size:
3478 2537 777 732 575 422
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8521
PvuII CAG'CTG
Cuts at: 3784 3784
Size: 8521
SacI G_AGCT'C
Cuts at: 4451 4451
Size: 8521
SalI G'TCGA_C
Cuts at: 1936 5863 1936
Size: 3927 4594
SbfI CC_TGCA'GG
Cuts at: 5873 5873
Size: 8521
ScaI AGT'ACT
Cuts at: 6769 8481 6769
Size: 1712 6809
SfcI C'TryA_G
Cuts at: 826 1045 3363 3424 3785 4074 4562 5294 5869
Size: 219 2318 61 361 289 488 732 575
Cuts at: 5869 7004 7685 7876 826
Size: 1135 681 191 1471
Fragments arranged by size:
2318 1471 1135 732 681 575 488 361
289 219 191 61
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8521
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8521
SmaI CCC'GGG
Cuts at: 1872 5848 1872
Size: 3976 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3722 6625 7526 7788 820
Size: 197 926 1779 2903 901 262 1553
Fragments arranged by size:
2903 1779 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5818 6312 493
Size: 5325 494 2702
Fragments arranged by size:
5325 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8521
SphI G_CATG'C
Cuts at: 1132 5879 1132
Size: 4747 3774
SspI AAT'ATT
Cuts at: 5 52 6445 5
Size: 47 6393 2081
Fragments arranged by size:
6393 2081 47
StuI AGG'CCT
Cuts at: 1517 3311 1517
Size: 1794 6727
StyI C'CwwG_G
Cuts at: 513 1065 2793 3010 3333 3477 4826 5715 5960
Size: 552 1728 217 323 144 1349 889 245
Cuts at: 5960 513
Size: 3074
Fragments arranged by size:
3074 1728 1349 889 552 323 245 217
144
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2171 2188 3036 6548 6565 6718 6903
Size: 1255 591 17 848 3512 17 153 185
Cuts at: 6903 7348 325
Size: 445 1498
Fragments arranged by size:
3512 1498 1255 848 591 445 185 153
17 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2565 2940 3132
Size: 274 80 33 51 1443 588 375 192
Cuts at: 3132 4290 6767 8479 96
Size: 1158 2477 1712 138
Fragments arranged by size:
2477 1712 1443 1158 588 375 274 192
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3015 3047 3655 3687 5522 5554 8481 8513 3015
Size: 32 608 32 1835 32 2927 32 3023
Fragments arranged by size:
3023 2927 1835 608 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2189 1316
Size: 873 7648
XbaI T'CTAG_A
Cuts at: 5857 5857
Size: 8521
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2458 3632 2458
Size: 1174 7347
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8521
XmaI C'CCGG_G
Cuts at: 1870 5846 1870
Size: 3976 4545
XmnI GAAnn'nnTTC
Cuts at: 6650 6650
Size: 8521
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2211 4219 6329 276
Size: 53 83 186 936 677 2008 2110 2468
Fragments arranged by size:
2468 2110 2008 936 677 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BsiWI BstZ17I EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PpuMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI