Restriction map for
pFN21ASDA1215
##################################
# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccIII AflII AflIII
AgeI AlwNI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AvrII BbrPI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BpuAI BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrDI
BsrGI BssHII Bst1107I BstAPI BstBI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII EagI EclXI Eco47III Eco52I
Eco81I EcoRV EcoT22I FbaI FseI
HincII HindII HindIII HpaI KpnI
KspI MaeI MamI MfeI MluI
MroI MunI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI PvuI RsrII SacI SacII
SalI SbfI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccI 853 GTCTAC GT^[AC][GT]AC
AclI 784 AACGTT AA^CGTT
Alw44I 685 GTGCAC G^TGCAC
ApaLI 685 GTGCAC G^TGCAC
AspI 289 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 766 GGCGCC GGCGC^C
BglI 337 GCCACCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1036 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1036 GCTGAGC GC^T[ACGT]AGC
BsgI 646 GTGCAG GTGCAG(16/14)
BspHI 1535 TCATGA T^CATGA
BspMI 875 ACCTGC ACCTGC(4/8)
BstEII 1401 GGTGACC G^GT[ACGT]ACC
BstPI 1401 GGTGACC G^GT[ACGT]ACC
CelII 1036 GCTGAGC GC^T[ACGT]AGC
DrdI 844 GACTTCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 927 CCTCGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1401 GGTGACC G^GT[ACGT]ACC
EcoRI 1273 GAATTC G^AATTC
HaeII 766 GGCGCC [GA]GCGC^[CT]
KasI 766 GGCGCC G^GCGCC
MaeII 785 ACGT A^CGT
NaeI 506 GCCGGC GCC^GGC
NarI 766 GGCGCC GG^CGCC
NgoMIV 506 GCCGGC G^CCGGC
PflFI 289 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1478 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 1003 GACAACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 784 AACGTT AA^CGTT
PvuII 486 CAGCTG CAG^CTG
RcaI 1535 TCATGA T^CATGA
SapI 591 GCTCTTC GCTCTTC(1/4)
SfoI 766 GGCGCC GGC^GCC
TaqI 102 TCGA T^CGA
Tth111I 289 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 102 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AccII 529 CGCG CG^CG
808 CGCG
AcsI 1273 GAATTC [GA]^AATT[CT]
1504 GAATTT
AhdI 4 GACACCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
163 GACAATGAGTC
ApoI 1273 GAATTC [GA]^AATT[CT]
1504 GAATTT
AspEI 4 GACACCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
163 GACAATGAGTC
AspHI 238 GAGCAC G[AT]GC[AT]^C
685 GTGCAC
AviII 605 TGCGCA TGC^GCA
1055 TGCGCA
BamHI 821 GGATCC G^GATCC
1512 GGATCC
BanI 229 GGCACC G^G[CT][GA]CC
766 GGCGCC
BanII 151 GAGCCC G[GA]GC[CT]^C
491 GGGCTC
BsaI 1552 GAGACC GGTCTC(1/5)
579 GGTCTC
BsiHKAI 238 GAGCAC G[AT]GC[AT]^C
685 GTGCAC
BsrBI 90 CCGCTC CCGCTC(-3/-3)
187 GAGCGG
BsrFI 506 GCCGGC [GA]^CCGG[CT]
1398 GCCGGT
BssSI 176 CACGAG CACGAG(-5/-1)
574 CTCGTG
BstUI 529 CGCG CG^CG
808 CGCG
BstXI 275 CCAAGGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
391 CCACTGCTGTGG
BstYI 821 GGATCC [GA]^GATC[CT]
1512 GGATCC
Cfr10I 506 GCCGGC [GA]^CCGG[CT]
1398 GCCGGT
DpnII 822 GATC ^GATC
1513 GATC
DraII 437 AGGGCCT [GA]G^G[ACGT]CC[CT]
1478 AGGTCCC
Eam1105I 4 GACACCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
163 GACAATGAGTC
EcoO109I 437 AGGGCCT [GA]G^G[ACGT]CC[CT]
1478 AGGTCCC
FspI 605 TGCGCA TGC^GCA
1055 TGCGCA
MboI 822 GATC ^GATC
1513 GATC
MflI 821 GGATCC [GA]^GATC[CT]
1512 GGATCC
MseI 1109 TTAA T^TAA
1438 TTAA
MslI 316 CACCTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
392 CACTGCTGTG
MvnI 529 CGCG CG^CG
808 CGCG
NcoI 1190 CCATGG C^CATGG
1247 CCATGG
NdeII 822 GATC ^GATC
1513 GATC
Sau3AI 822 GATC ^GATC
1513 GATC
ScaI 17 AGTACT AGT^ACT
1019 AGTACT
SmaI 145 CCCGGG CCC^GGG
1160 CCCGGG
SmlI 742 CTCAAG C^T[CT][GA]AG
1414 CTCAAG
Tru9I 1109 TTAA T^TAA
1438 TTAA
Tsp509I 1274 AATT ^AATT
1505 AATT
XhoII 821 GGATCC [GA]^GATC[CT]
1512 GGATCC
XmaCI 145 CCCGGG C^CCGGG
1160 CCCGGG
XmaI 145 CCCGGG C^CCGGG
1160 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AvaI AvaII BalI BbvI BcnI
BmyI BpmI BsaHI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DsaI
EaeI EarI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeIII MboII
MluNI MnlI MscI MspA1I MspI
MvaI MwoI NciI NlaIII NlaIV
PflMI PleI PstI RsaI Sau96I
ScrFI SfaNI SfcI StuI StyI
TseI Tsp45I TspRI Van91I XcmI