(Circular) (Six-base) MAPSORT of: pFN21ASDA1215.seq Check: 6205 from: 1 to: 6292 pFN21ASDA1215 6292 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4102 278 Size: 53 83 186 936 2566 2468 Fragments arranged by size: 2566 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2877 3635 1937 Size: 940 758 4594 Fragments arranged by size: 4594 940 758 Acc65I G'GTAC_C Cuts at: 3613 3613 Size: 6292 AclI AA'CG_TT Cuts at: 1563 2808 4419 4792 1563 Size: 1245 1611 373 3063 Fragments arranged by size: 3063 1611 1245 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3460 4355 5739 6007 1632 Size: 1828 895 1384 268 1917 Fragments arranged by size: 1917 1828 1384 895 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6095 AflIII A'CryG_T Cuts at: 1203 5191 6168 1203 Size: 3988 977 1327 Fragments arranged by size: 3988 1327 977 AgeI A'CCGG_T Cuts at: 5123 5123 Size: 6292 AhdI GACnn_n'nnGTC Cuts at: 2032 2191 5021 2032 Size: 159 2830 3303 Fragments arranged by size: 3303 2830 159 AleI CACnn'nnGTG Cuts at: 1166 2343 2419 1166 Size: 1177 76 5039 Fragments arranged by size: 5039 1177 76 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6292 AlwNI CAG_nnn'CTG Cuts at: 4035 5607 4035 Size: 1572 4720 ApaLI G'TGCA_C Cuts at: 2708 4349 5505 2708 Size: 1641 1156 3495 Fragments arranged by size: 3495 1641 1156 ApoI r'AATT_y Cuts at: 1493 3296 3527 3601 3827 3979 6088 1493 Size: 1803 231 74 226 152 2109 1697 Fragments arranged by size: 2109 1803 1697 231 226 152 74 AseI AT'TA_AT Cuts at: 160 1032 4846 160 Size: 872 3814 1606 Fragments arranged by size: 3814 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6292 AvaI C'yCGr_G Cuts at: 1870 1943 2044 2168 2643 2991 3183 3617 1870 Size: 73 101 124 475 348 192 434 4545 Fragments arranged by size: 4545 475 434 348 192 124 101 73 BamHI G'GATC_C Cuts at: 2844 3535 3622 3989 2844 Size: 691 87 367 5147 Fragments arranged by size: 5147 691 367 87 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2252 2789 3613 5068 618 Size: 325 217 627 465 537 824 1455 1842 Fragments arranged by size: 1842 1455 824 627 537 465 325 217 BanII G_rGCy'C Cuts at: 1391 2178 2518 3611 1391 Size: 787 340 1093 4072 Fragments arranged by size: 4072 1093 787 340 BbeI G_GCGC'C Cuts at: 2793 2793 Size: 6292 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5321 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4483 4517 1903 Size: 34 2546 34 3678 Fragments arranged by size: 3678 2546 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4184 5394 4184 Size: 1210 5082 BclI T'GATC_A Cuts at: 1548 1548 Size: 6292 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2366 4903 136 Size: 107 122 71 631 605 694 2537 1525 Fragments arranged by size: 2537 1525 694 631 605 122 107 71 BglII A'GATC_T Cuts at: 6287 6287 Size: 6292 BlpI GC'TnA_GC Cuts at: 3060 3709 3060 Size: 649 5643 Bme1580I G_kGCm'C Cuts at: 1790 2255 2572 2712 4353 5509 1790 Size: 465 317 140 1641 1156 2573 Fragments arranged by size: 2573 1641 1156 465 317 140 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6292 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2032 2233 2513 Size: 416 53 83 399 24 615 201 280 Cuts at: 2513 4023 4981 442 Size: 1510 958 1753 Fragments arranged by size: 1753 1510 958 615 416 399 280 201 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6292 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2456 2732 2753 2870 4952 1239 Size: 1217 276 21 117 2082 2579 Fragments arranged by size: 2579 2082 1217 276 117 21 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2750 3422 4417 5282 5580 2750 Size: 672 995 865 298 3462 Fragments arranged by size: 3462 995 865 672 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2608 3569 4955 882 Size: 617 1109 961 1386 2219 Fragments arranged by size: 2219 1386 1109 961 617 BsaAI yAC'GTr Cuts at: 493 1204 3589 4083 493 Size: 711 2385 494 2702 Fragments arranged by size: 2702 2385 711 494 BsaBI GATnn'nnATC Cuts at: 6286 6286 Size: 6292 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2694 2790 3320 4099 Size: 53 83 186 936 1161 96 530 779 Cuts at: 4099 4481 5116 275 Size: 382 635 1451 Fragments arranged by size: 1451 1161 936 779 635 530 382 186 96 83 53 BsaWI w'CCGG_w Cuts at: 4725 5123 5397 5544 5955 4725 Size: 398 274 147 411 5062 Fragments arranged by size: 5062 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2255 2285 1178 Size: 30 165 30 852 30 5185 Fragments arranged by size: 5185 852 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2444 2882 3068 3100 3128 3164 3431 1186 Size: 1258 438 186 32 28 36 267 4047 Fragments arranged by size: 4047 1258 438 267 186 36 32 28 BseYI C'CCAG_C Cuts at: 2511 5495 5991 2511 Size: 2984 496 2812 Fragments arranged by size: 2984 2812 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2690 1163 Size: 573 954 4765 Fragments arranged by size: 4765 954 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3742 4503 5531 1540 Size: 275 186 1741 761 1028 2301 Fragments arranged by size: 2301 1741 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2265 2712 4353 4438 5509 2265 Size: 447 1641 85 1071 3048 Fragments arranged by size: 3048 1641 1071 447 85 BsmI GAATG_Cn' Cuts at: 3803 3896 3803 Size: 93 6199 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2178 2255 2265 2518 2572 2712 3611 Size: 399 388 77 10 253 54 140 899 Cuts at: 3611 4353 4438 5509 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 899 742 399 388 253 140 85 77 54 10 BspEI T'CCGG_A Cuts at: 5955 5955 Size: 6292 BspHI T'CATG_A Cuts at: 3558 4181 5911 3558 Size: 623 1730 3939 Fragments arranged by size: 3939 1730 623 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2907 3647 844 Size: 2063 740 3489 Fragments arranged by size: 3489 2063 740 BsrBI CCG'CTC Cuts at: 1394 1823 2115 2212 4179 1394 Size: 429 292 97 1967 3507 Fragments arranged by size: 3507 1967 429 292 97 BsrDI GCAATG_nn' Cuts at: 65 1248 4787 4961 65 Size: 1183 3539 174 1396 Fragments arranged by size: 3539 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2529 3421 4936 5123 1700 Size: 110 719 892 1515 187 2869 Fragments arranged by size: 2869 1515 892 719 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6292 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6292 BssSI C'ACGA_G Cuts at: 2199 2597 4352 5364 2199 Size: 398 1755 1012 3127 Fragments arranged by size: 3127 1755 1012 398 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6292 BstBI TT'CG_AA Cuts at: 2007 5096 2007 Size: 3089 3203 BstEII G'GTnAC_C Cuts at: 1184 3424 1184 Size: 2240 4052 BstXI CCAn_nnnn'nTGG Cuts at: 2305 2421 2305 Size: 116 6176 BstYI r'GATC_y Cuts at: 1972 2844 3535 3622 3989 4374 4391 5843 5929 Size: 872 691 87 367 385 17 1452 86 Cuts at: 5929 5941 6287 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 872 691 385 367 346 87 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2721 3213 3270 513 Size: 552 957 699 492 57 3535 Fragments arranged by size: 3535 957 699 552 492 57 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2278 487 Size: 683 56 1052 4501 Fragments arranged by size: 4501 1052 683 56 BtsI GCAGTG_nn' Cuts at: 2369 2414 2844 3808 4602 4622 2369 Size: 45 430 964 794 20 4039 Fragments arranged by size: 4039 964 794 430 45 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6292 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6257 DraI TTT'AAA Cuts at: 3596 3596 Size: 6292 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2873 5299 809 Size: 507 1557 2426 1802 Fragments arranged by size: 2426 1802 1557 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2718 2812 2916 Size: 54 1425 50 275 18 888 94 104 Cuts at: 2916 2983 3390 3739 4628 6257 8 Size: 67 407 349 889 1629 43 Fragments arranged by size: 1629 1425 889 888 407 349 275 104 94 67 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3739 1537 Size: 275 1927 4090 Fragments arranged by size: 4090 1927 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2471 2621 3141 4222 1353 Size: 1118 150 520 1081 3423 Fragments arranged by size: 3423 1118 1081 520 150 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2516 4879 5253 5399 387 Size: 1064 138 927 2363 374 146 1280 Fragments arranged by size: 2363 1280 1064 927 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2456 2732 2753 2870 3460 4355 4952 Size: 393 824 276 21 117 590 895 597 Cuts at: 4952 5739 6007 1239 Size: 787 268 1524 Fragments arranged by size: 1524 895 824 787 597 590 393 276 268 117 21 EcoNI CCTnn'n_nnAGG Cuts at: 2954 2954 Size: 6292 EcoO109I rG'GnC_Cy Cuts at: 2461 3502 2461 Size: 1041 5251 EcoRI G'AATT_C Cuts at: 3296 3601 3979 3296 Size: 305 378 5609 Fragments arranged by size: 5609 378 305 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3349 3349 Size: 6292 FspI TGC'GCA Cuts at: 2630 3080 4798 2630 Size: 450 1718 4124 Fragments arranged by size: 4124 1718 450 FspAI rTGC'GCAy Cuts at: 2630 2630 Size: 6292 HaeII r_GCGC'y Cuts at: 1678 2793 5439 1678 Size: 1115 2646 2531 Fragments arranged by size: 2646 2531 1115 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2018 2050 2177 2209 3348 Size: 32 135 32 446 32 127 32 1139 Cuts at: 3348 3380 3527 3559 4933 4965 5007 5039 1373 Size: 32 147 32 1374 32 42 32 2626 Fragments arranged by size: 2626 1374 1139 446 147 135 127 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3636 3882 669 Size: 988 281 1698 246 3079 Fragments arranged by size: 3079 1698 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6292 HpaI GTT'AAC Cuts at: 3882 3882 Size: 6292 KasI G'GCGC_C Cuts at: 2789 2789 Size: 6292 KpnI G_GTAC'C Cuts at: 3617 3617 Size: 6292 MfeI C'AATT_G Cuts at: 3891 3891 Size: 6292 MluI A'CGCG_T Cuts at: 6168 6168 Size: 6292 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5406 5590 1305 Size: 4101 184 2007 Fragments arranged by size: 4101 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2814 2918 2985 3392 10 Size: 54 1425 343 982 104 67 407 2910 Fragments arranged by size: 2910 1425 982 407 343 104 67 54 MslI CAynn'nnrTG Cuts at: 518 1166 2343 2419 4250 4609 4768 518 Size: 648 1177 76 1831 359 159 2042 Fragments arranged by size: 2042 1831 1177 648 359 159 76 MspA1I CmG'CkG Cuts at: 1606 2354 2511 3008 3708 4385 5533 5778 1606 Size: 748 157 497 700 677 1148 245 2120 Fragments arranged by size: 2120 1148 748 700 677 497 245 157 NaeI GCC'GGC Cuts at: 2531 2531 Size: 6292 NarI GG'CG_CC Cuts at: 2790 2790 Size: 6292 NcoI C'CATG_G Cuts at: 513 1065 2022 3213 3270 513 Size: 552 957 1191 57 3535 Fragments arranged by size: 3535 1191 957 552 57 NdeI CA'TA_TG Cuts at: 387 387 Size: 6292 NgoMIV G'CCGG_C Cuts at: 2529 2529 Size: 6292 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6292 NotI GC'GGCC_GC Cuts at: 3739 3739 Size: 6292 NspI r_CATG'y Cuts at: 1132 1229 3650 5195 1132 Size: 97 2421 1545 2229 Fragments arranged by size: 2421 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3165 1348 Size: 1817 4475 PciI A'CATG_T Cuts at: 5191 5191 Size: 6292 PflMI CCAn_nnn'nTGG Cuts at: 1108 2665 2833 3271 3399 1108 Size: 1557 168 438 128 4001 Fragments arranged by size: 4001 1557 438 168 128 PfoI T'CCnGG_A Cuts at: 2634 2847 2634 Size: 213 6079 PmeI GTTT'AAAC Cuts at: 3596 3596 Size: 6292 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4361 4393 5874 5906 4361 Size: 32 1481 32 4747 Fragments arranged by size: 4747 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3502 3502 Size: 6292 PshAI GACnn'nnGTC Cuts at: 1613 3030 1613 Size: 1417 4875 PsiI TTA'TAA Cuts at: 3862 3862 Size: 6292 PstI C_TGCA'G Cuts at: 830 3144 3288 3570 3644 830 Size: 2314 144 282 74 3478 Fragments arranged by size: 3478 2314 282 144 74 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6292 PvuII CAG'CTG Cuts at: 2511 2511 Size: 6292 SalI G'TCGA_C Cuts at: 1936 3634 1936 Size: 1698 4594 SapI GCTCTTCn'nnn_ Cuts at: 2621 2621 Size: 6292 SbfI CC_TGCA'GG Cuts at: 3644 3644 Size: 6292 ScaI AGT'ACT Cuts at: 2042 3044 4540 6252 2042 Size: 1002 1496 1712 2082 Fragments arranged by size: 2082 1712 1496 1002 SfcI C'TryA_G Cuts at: 826 1045 3140 3284 3566 3640 4775 5456 5647 Size: 219 2095 144 282 74 1135 681 191 Cuts at: 5647 826 Size: 1471 Fragments arranged by size: 2095 1471 1135 681 282 219 191 144 74 SfoI GGC'GCC Cuts at: 2791 2791 Size: 6292 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6292 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6292 SmaI CCC'GGG Cuts at: 1872 2170 3185 3619 1872 Size: 298 1015 434 4545 Fragments arranged by size: 4545 1015 434 298 SmlI C'TyrA_G Cuts at: 820 1017 1943 2765 3437 4396 5297 5559 820 Size: 197 926 822 672 959 901 262 1553 Fragments arranged by size: 1553 959 926 901 822 672 262 197 SnaBI TAC'GTA Cuts at: 493 3589 4083 493 Size: 3096 494 2702 Fragments arranged by size: 3096 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6292 SphI G_CATG'C Cuts at: 1132 3650 1132 Size: 2518 3774 SspI AAT'ATT Cuts at: 5 52 4216 5 Size: 47 4164 2081 Fragments arranged by size: 4164 2081 47 StuI AGG'CCT Cuts at: 1517 2540 3119 3329 1517 Size: 1023 579 210 4480 Fragments arranged by size: 4480 1023 579 210 StyI C'CwwG_G Cuts at: 513 1065 2022 2298 2535 3213 3270 3324 3731 Size: 552 957 276 237 678 57 54 407 Cuts at: 3731 513 Size: 3074 Fragments arranged by size: 3074 957 678 552 407 276 237 57 54 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2121 2333 4319 4336 4489 4674 5119 Size: 1255 541 212 1986 17 153 185 445 Cuts at: 5119 325 Size: 1498 Fragments arranged by size: 1986 1498 1255 541 445 212 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2040 3042 4538 Size: 274 80 33 51 1443 63 1002 1496 Cuts at: 4538 6250 96 Size: 1712 138 Fragments arranged by size: 1712 1496 1443 1002 274 138 80 63 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2255 2287 6252 6284 2255 Size: 32 3965 32 2263 Fragments arranged by size: 3965 2263 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2315 1316 Size: 999 5293 XbaI T'CTAG_A Cuts at: 3628 3628 Size: 6292 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2911 3100 3172 3331 2911 Size: 189 72 159 5872 Fragments arranged by size: 5872 189 159 72 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6292 XmaI C'CCGG_G Cuts at: 1870 2168 3183 3617 1870 Size: 298 1015 434 4545 Fragments arranged by size: 4545 1015 434 298 XmnI GAAnn'nnTTC Cuts at: 4421 4421 Size: 6292 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4100 276 Size: 53 83 186 936 2566 2468 Fragments arranged by size: 2566 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI