Restriction map for
pFN21ASDA1215
(Linear) (Six-base) MAPSORT of: KIAA1215.seq Check: 5430 from: 1 to: 1563
KIAA1215.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AccI GT'mk_AC
Cuts at: 0 854 1563
Size: 854 709
AclI AA'CG_TT
Cuts at: 0 785 1563
Size: 785 778
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1437 1563
Size: 1437 126
AhdI GACnn_n'nnGTC
Cuts at: 0 9 168 1563
Size: 9 159 1395
Fragments arranged by size:
1395 159 9
AleI CACnn'nnGTG
Cuts at: 0 320 396 1563
Size: 320 76 1167
Fragments arranged by size:
1167 320 76
ApaLI G'TGCA_C
Cuts at: 0 685 1563
Size: 685 878
ApoI r'AATT_y
Cuts at: 0 1273 1504 1563
Size: 1273 231 59
Fragments arranged by size:
1273 231 59
AvaI C'yCGr_G
Cuts at: 0 21 145 620 968 1160 1563
Size: 21 124 475 348 192 403
Fragments arranged by size:
475 403 348 192 124 21
BamHI G'GATC_C
Cuts at: 0 821 1512 1563
Size: 821 691 51
Fragments arranged by size:
821 691 51
BanI G'GyrC_C
Cuts at: 0 229 766 1563
Size: 229 537 797
Fragments arranged by size:
797 537 229
BanII G_rGCy'C
Cuts at: 0 155 495 1563
Size: 155 340 1068
Fragments arranged by size:
1068 340 155
BbeI G_GCGC'C
Cuts at: 0 770 1563
Size: 770 793
BglI GCCn_nnn'nGGC
Cuts at: 0 343 1563
Size: 343 1220
BlpI GC'TnA_GC
Cuts at: 0 1037 1563
Size: 1037 526
Bme1580I G_kGCm'C
Cuts at: 0 232 549 689 1563
Size: 232 317 140 874
Fragments arranged by size:
874 317 232 140
BmrI ACTGGGnnnn_n'
Cuts at: 0 9 210 490 1563
Size: 9 201 280 1073
Fragments arranged by size:
1073 280 201 9
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 433 709 730 847 1563
Size: 433 276 21 117 716
Fragments arranged by size:
716 433 276 117 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 727 1399 1563
Size: 727 672 164
Fragments arranged by size:
727 672 164
BsaI GGTCTCn'nnnn_
Cuts at: 0 585 1546 1563
Size: 585 961 17
Fragments arranged by size:
961 585 17
BsaHI Gr'CG_yC
Cuts at: 0 671 767 1297 1563
Size: 671 96 530 266
Fragments arranged by size:
671 530 266 96
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 232 262 1563
Size: 232 30 1301
Fragments arranged by size:
1301 232 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 421 859 1045 1077 1105 1141 1408 1563
Size: 421 438 186 32 28 36 267 155
Fragments arranged by size:
438 421 267 186 155 36 32 28
BseYI C'CCAG_C
Cuts at: 0 488 1563
Size: 488 1075
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 667 1563
Size: 667 896
BsiHKAI G_wGCw'C
Cuts at: 0 242 689 1563
Size: 242 447 874
Fragments arranged by size:
874 447 242
Bsp1286I G_dGCh'C
Cuts at: 0 155 232 242 495 549 689 1563
Size: 155 77 10 253 54 140 874
Fragments arranged by size:
874 253 155 140 77 54 10
BspHI T'CATG_A
Cuts at: 0 1535 1563
Size: 1535 28
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 884 1563
Size: 884 679
BsrBI CCG'CTC
Cuts at: 0 92 189 1563
Size: 92 97 1374
Fragments arranged by size:
1374 97 92
BsrFI r'CCGG_y
Cuts at: 0 506 1398 1563
Size: 506 892 165
Fragments arranged by size:
892 506 165
BssSI C'ACGA_G
Cuts at: 0 176 574 1563
Size: 176 398 989
Fragments arranged by size:
989 398 176
BstEII G'GTnAC_C
Cuts at: 0 1401 1563
Size: 1401 162
BstXI CCAn_nnnn'nTGG
Cuts at: 0 282 398 1563
Size: 282 116 1165
Fragments arranged by size:
1165 282 116
BstYI r'GATC_y
Cuts at: 0 821 1512 1563
Size: 821 691 51
Fragments arranged by size:
821 691 51
BtgI C'CryG_G
Cuts at: 0 698 1190 1247 1563
Size: 698 492 57 316
Fragments arranged by size:
698 492 316 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 255 1563
Size: 255 1308
BtsI GCAGTG_nn'
Cuts at: 0 346 391 821 1563
Size: 346 45 430 742
Fragments arranged by size:
742 430 346 45
DrdI GACnn_nn'nnGTC
Cuts at: 0 850 1563
Size: 850 713
EaeI y'GGCC_r
Cuts at: 0 695 789 893 960 1367 1563
Size: 695 94 104 67 407 196
Fragments arranged by size:
695 407 196 104 94 67
EarI CTCTTCn'nnn_
Cuts at: 0 448 598 1118 1563
Size: 448 150 520 445
Fragments arranged by size:
520 448 445 150
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 493 1563
Size: 493 1070
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 433 709 730 847 1437 1563
Size: 433 276 21 117 590 126
Fragments arranged by size:
590 433 276 126 117 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 931 1563
Size: 931 632
EcoO109I rG'GnC_Cy
Cuts at: 0 438 1479 1563
Size: 438 1041 84
Fragments arranged by size:
1041 438 84
EcoRI G'AATT_C
Cuts at: 0 1273 1563
Size: 1273 290
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1326 1563
Size: 1326 237
FspI TGC'GCA
Cuts at: 0 607 1057 1563
Size: 607 450 506
Fragments arranged by size:
607 506 450
FspAI rTGC'GCAy
Cuts at: 0 607 1563
Size: 607 956
HaeII r_GCGC'y
Cuts at: 0 770 1563
Size: 770 793
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 27 154 186 1325 1357 1504 1536 1563
Size: 27 127 32 1139 32 147 32 27
Fragments arranged by size:
1139 147 127 32 32 32 27 27
KasI G'GCGC_C
Cuts at: 0 766 1563
Size: 766 797
MscI TGG'CCA
Cuts at: 0 791 895 962 1369 1563
Size: 791 104 67 407 194
Fragments arranged by size:
791 407 194 104 67
MslI CAynn'nnrTG
Cuts at: 0 320 396 1563
Size: 320 76 1167
Fragments arranged by size:
1167 320 76
MspA1I CmG'CkG
Cuts at: 0 331 488 985 1563
Size: 331 157 497 578
Fragments arranged by size:
578 497 331 157
NaeI GCC'GGC
Cuts at: 0 508 1563
Size: 508 1055
NarI GG'CG_CC
Cuts at: 0 767 1563
Size: 767 796
NcoI C'CATG_G
Cuts at: 0 1190 1247 1563
Size: 1190 57 316
Fragments arranged by size:
1190 316 57
NgoMIV G'CCGG_C
Cuts at: 0 506 1563
Size: 506 1057
PasI CC'CwG_GG
Cuts at: 0 1142 1563
Size: 1142 421
PflMI CCAn_nnn'nTGG
Cuts at: 0 642 810 1248 1376 1563
Size: 642 168 438 128 187
Fragments arranged by size:
642 438 187 168 128
PfoI T'CCnGG_A
Cuts at: 0 611 824 1563
Size: 611 213 739
Fragments arranged by size:
739 611 213
PpuMI rG'GwC_Cy
Cuts at: 0 1479 1563
Size: 1479 84
PshAI GACnn'nnGTC
Cuts at: 0 1007 1563
Size: 1007 556
PstI C_TGCA'G
Cuts at: 0 1121 1265 1547 1563
Size: 1121 144 282 16
Fragments arranged by size:
1121 282 144 16
PvuII CAG'CTG
Cuts at: 0 488 1563
Size: 488 1075
SapI GCTCTTCn'nnn_
Cuts at: 0 598 1563
Size: 598 965
ScaI AGT'ACT
Cuts at: 0 19 1021 1563
Size: 19 1002 542
Fragments arranged by size:
1002 542 19
SfcI C'TryA_G
Cuts at: 0 1117 1261 1543 1563
Size: 1117 144 282 20
Fragments arranged by size:
1117 282 144 20
SfoI GGC'GCC
Cuts at: 0 768 1563
Size: 768 795
SmaI CCC'GGG
Cuts at: 0 147 1162 1563
Size: 147 1015 401
Fragments arranged by size:
1015 401 147
SmlI C'TyrA_G
Cuts at: 0 742 1414 1563
Size: 742 672 149
Fragments arranged by size:
742 672 149
StuI AGG'CCT
Cuts at: 0 517 1096 1306 1563
Size: 517 579 210 257
Fragments arranged by size:
579 517 257 210
StyI C'CwwG_G
Cuts at: 0 275 512 1190 1247 1301 1563
Size: 275 237 678 57 54 262
Fragments arranged by size:
678 275 262 237 57 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 98 310 1563
Size: 98 212 1253
Fragments arranged by size:
1253 212 98
TatI w'GTAC_w
Cuts at: 0 17 1019 1563
Size: 17 1002 544
Fragments arranged by size:
1002 544 17
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 232 264 1563
Size: 232 32 1299
Fragments arranged by size:
1299 232 32
Tth111I GACn'n_nGTC
Cuts at: 0 292 1563
Size: 292 1271
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 888 1077 1149 1308 1563
Size: 888 189 72 159 255
Fragments arranged by size:
888 255 189 159 72
XmaI C'CCGG_G
Cuts at: 0 145 1160 1563
Size: 145 1015 403
Fragments arranged by size:
1015 403 145
Enzymes that do cut:
AccI AclI AcuI AhdI AleI ApaLI ApoI AvaI
BamHI BanI BanII BbeI BglI BlpI Bme1580I BmrI
BpmI BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrFI BssSI BstEII
BstXI BstYI BtgI BtgZI BtsI DrdI EaeI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I KasI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV PasI PflMI PfoI PpuMI PshAI PstI
PvuII SapI ScaI SfcI SfoI SmaI SmlI StuI
StyI TaqII TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AflIII AgeI AlfI
AloI AlwNI ApaI AscI AseI AsiSI AvrII BaeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglII BmgBI
BmtI BplI Bpu10I BsaAI BsaBI BsaWI BsiEI BsiWI
BsmI BsmBI BspEI BsrDI BsrGI BssHII BstAPI BstBI
BstZ17I Bsu36I ClaI CspCI DraI DraIII EagI EcoICRI
EcoRV FseI HincII HindIII HpaI KpnI MfeI MluI
MmeI NdeI NheI NotI NruI NsiI NspI PacI
PciI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SbfI SexAI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI XbaI XhoI XmnI ZraI