Restriction map for
pFN21ASDA1214
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# NO CUT ENZYMES ( 116 enzymes) #
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Acc65I AccI AclI AcsI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AviII BbeI
BbrPI BfrI BglII BlpI Bpu1102I
BsaBI BseRI BsiWI BsmI BspDI
BspHI BspLU11I BsrBI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraII DraIII EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HindIII
HpaI KasI KpnI Ksp632I MamI
MfeI MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI SapI SbfI ScaI
SexAI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tsp509I Van91I XcmI
XhoI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 1051 GACGTC GACGT^C
AflIII 401 ACGCGT A^C[GA][CT]GT
AlwNI 686 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 399 GAACGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 235 GAGCAC G[AT]GC[AT]^C
AspI 739 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 163 CCCGGG C^[CT]CG[GA]G
AvrII 849 CCTAGG C^CTAGG
BanI 940 GGTGCC G^G[CT][GA]CC
BbvCI 419 CCTCAGC CCTCAGC(-5/-2)
BcgI 600 GCAGAAATATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1023 GGATAC GTATCC(6/5)
BclI 1115 TGATCA T^GATCA
BlnI 849 CCTAGG C^CTAGG
BpmI 649 CTCCAG CTGGAG(16/14)
BsaAI 378 CACGTA [CT]AC^GT[GA]
BsaI 460 GAGACC GGTCTC(1/5)
BsiEI 291 CGACCG CG[GA][CT]^CG
BsiHKAI 235 GAGCAC G[AT]GC[AT]^C
BsmBI 1049 GAGACG CGTCTC(1/5)
BsmFI 818 GTCCC GGGAC(10/14)
BsoBI 163 CCCGGG C^[CT]CG[GA]G
Bsp1407I 572 TGTACA T^GTACA
BspMI 1011 GCAGGT ACCTGC(4/8)
BsrFI 761 GCCGGC [GA]^CCGG[CT]
BsrGI 572 TGTACA T^GTACA
Cfr10I 761 GCCGGC [GA]^CCGG[CT]
DrdI 982 GACCCTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 1115 TGATCA T^GATCA
HaeII 224 GGCGCT [GA]GCGC^[CT]
HgaI 253 GACGC GACGC(5/10)
HincII 787 GTTGAC GT[CT]^[GA]AC
HindII 787 GTTGAC GT[CT]^[GA]AC
MluI 401 ACGCGT A^CGCGT
MseI 842 TTAA T^TAA
NaeI 761 GCCGGC GCC^GGC
NgoMIV 761 GCCGGC G^CCGGC
PflFI 739 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 688 GAGTC GAGTC(4/5)
PvuII 1085 CAGCTG CAG^CTG
SfiI 276 GGCCAGCTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 163 CCCGGG CCC^GGG
SspBI 572 TGTACA T^GTACA
TaqI 899 TCGA T^CGA
Tru9I 842 TTAA T^TAA
Tth111I 739 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 899 TCGA T^CGA
XbaI 801 TCTAGA T^CTAGA
XmaCI 163 CCCGGG C^CCGGG
XmaI 163 CCCGGG C^CCGGG
XmnI 399 GAACGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AcyI 253 GACGCC G[GA]^CG[CT]C
1051 GACGTC
BalI 275 TGGCCA TGG^CCA
500 TGGCCA
BamHI 749 GGATCC G^GATCC
854 GGATCC
BbsI 210 GAAGAC GAAGAC(2/6)
490 GAAGAC
BfaI 802 CTAG C^TAG
850 CTAG
BpuAI 210 GAAGAC GAAGAC(2/6)
490 GAAGAC
BsaHI 253 GACGCC G[GA]^CG[CT]C
1051 GACGTC
BsrDI 4 GCAATG GCAATG(2/0)
1123 GCAATG
BsrI 347 ACTGG ACTGG(1/-1)
615 ACTGG
DdeI 420 CTCAG C^T[ACGT]AG
515 CTGAG
EcoT14I 365 CCAAGG C^C[AT][AT]GG
849 CCTAGG
Hin1I 253 GACGCC G[GA]^CG[CT]C
1051 GACGTC
KspI 258 CCGCGG CCGC^GG
336 CCGCGG
MaeI 802 CTAG C^TAG
850 CTAG
MluNI 275 TGGCCA TGG^CCA
500 TGGCCA
MscI 275 TGGCCA TGG^CCA
500 TGGCCA
PstI 27 CTGCAG CTGCA^G
409 CTGCAG
SacII 258 CCGCGG CCGC^GG
336 CCGCGG
SfcI 27 CTGCAG C^T[GA][CT]AG
409 CTGCAG
StyI 365 CCAAGG C^C[AT][AT]GG
849 CCTAGG
TfiI 510 GATTC G^A[AT]TC
1099 GATTC
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# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AccIII AciI AfaI AluI
AlwI AvaII BanII BbvI BcnI
BglI BmyI BsaJI BsaWI BseAI
BsgI BsiYI BslI BsmAI Bsp1286I
BspEI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DpnII
DsaI EaeI EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MnlI
MroI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI TseI Tsp45I TspRI XhoII