Restriction map for
pFN21ASDA1214
(Linear) (Six-base) MAPSORT of: KIAA1214.seq Check: 4358 from: 1 to: 1188
KIAA1214.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1002 1188
Size: 1002 186
AatII G_ACGT'C
Cuts at: 0 1055 1188
Size: 1055 133
AflIII A'CryG_T
Cuts at: 0 401 1188
Size: 401 787
AleI CACnn'nnGTG
Cuts at: 0 482 955 1188
Size: 482 473 233
Fragments arranged by size:
482 473 233
AlwNI CAG_nnn'CTG
Cuts at: 0 691 1188
Size: 691 497
AvaI C'yCGr_G
Cuts at: 0 163 1188
Size: 163 1025
AvrII C'CTAG_G
Cuts at: 0 849 1188
Size: 849 339
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 795 828 1188
Size: 795 33 360
Fragments arranged by size:
795 360 33
BamHI G'GATC_C
Cuts at: 0 749 854 1188
Size: 749 105 334
Fragments arranged by size:
749 334 105
BanI G'GyrC_C
Cuts at: 0 940 1188
Size: 940 248
BanII G_rGCy'C
Cuts at: 0 152 448 907 1188
Size: 152 296 459 281
Fragments arranged by size:
459 296 281 152
BbsI GAAGACnn'nnnn_
Cuts at: 0 217 497 1188
Size: 217 280 691
Fragments arranged by size:
691 280 217
BbvCI CC'TCA_GC
Cuts at: 0 420 1188
Size: 420 768
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 589 623 1188
Size: 589 34 565
Fragments arranged by size:
589 565 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1017 1188
Size: 1017 171
BclI T'GATC_A
Cuts at: 0 1115 1188
Size: 1115 73
BglI GCCn_nnn'nGGC
Cuts at: 0 175 283 762 1188
Size: 175 108 479 426
Fragments arranged by size:
479 426 175 108
BmrI ACTGGGnnnn_n'
Cuts at: 0 625 1188
Size: 625 563
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 634 1188
Size: 634 554
Bpu10I CC'TnA_GC
Cuts at: 0 420 515 1188
Size: 420 95 673
Fragments arranged by size:
673 420 95
BsaI GGTCTCn'nnnn_
Cuts at: 0 454 1188
Size: 454 734
BsaAI yAC'GTr
Cuts at: 0 380 1188
Size: 380 808
BsaHI Gr'CG_yC
Cuts at: 0 254 1052 1188
Size: 254 798 136
Fragments arranged by size:
798 254 136
BsaWI w'CCGG_w
Cuts at: 0 395 746 992 1188
Size: 395 351 246 196
Fragments arranged by size:
395 351 246 196
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 100 130 430 460 1188
Size: 100 30 300 30 728
Fragments arranged by size:
728 300 100 30 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 360 728 1188
Size: 184 176 368 460
Fragments arranged by size:
460 368 184 176
BsiEI CG_ry'CG
Cuts at: 0 294 1188
Size: 294 894
BsiHKAI G_wGCw'C
Cuts at: 0 239 1188
Size: 239 949
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1043 1188
Size: 1043 145
Bsp1286I G_dGCh'C
Cuts at: 0 152 239 448 907 1188
Size: 152 87 209 459 281
Fragments arranged by size:
459 281 209 152 87
BspEI T'CCGG_A
Cuts at: 0 395 746 992 1188
Size: 395 351 246 196
Fragments arranged by size:
395 351 246 196
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1002 1188
Size: 1002 186
BsrDI GCAATG_nn'
Cuts at: 0 11 1130 1188
Size: 11 1119 58
Fragments arranged by size:
1119 58 11
BsrFI r'CCGG_y
Cuts at: 0 761 1188
Size: 761 427
BsrGI T'GTAC_A
Cuts at: 0 572 1188
Size: 572 616
BstYI r'GATC_y
Cuts at: 0 392 749 854 1188
Size: 392 357 105 334
Fragments arranged by size:
392 357 334 105
BtgI C'CryG_G
Cuts at: 0 95 258 336 425 1188
Size: 95 163 78 89 763
Fragments arranged by size:
763 163 95 89 78
BtsI GCAGTG_nn'
Cuts at: 0 1101 1188
Size: 1101 87
DrdI GACnn_nn'nnGTC
Cuts at: 0 988 1188
Size: 988 200
EaeI y'GGCC_r
Cuts at: 0 98 275 500 1188
Size: 98 177 225 688
Fragments arranged by size:
688 225 177 98
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 634 1188
Size: 634 554
HaeII r_GCGC'y
Cuts at: 0 228 1188
Size: 228 960
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1040 1072 1188
Size: 1040 32 116
Fragments arranged by size:
1040 116 32
HincII GTy'rAC
Cuts at: 0 789 1188
Size: 789 399
MluI A'CGCG_T
Cuts at: 0 401 1188
Size: 401 787
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 473 1113 1188
Size: 473 640 75
Fragments arranged by size:
640 473 75
MscI TGG'CCA
Cuts at: 0 277 502 1188
Size: 277 225 686
Fragments arranged by size:
686 277 225
MslI CAynn'nnrTG
Cuts at: 0 469 482 562 955 1123 1188
Size: 469 13 80 393 168 65
Fragments arranged by size:
469 393 168 80 65 13
MspA1I CmG'CkG
Cuts at: 0 260 338 1087 1188
Size: 260 78 749 101
Fragments arranged by size:
749 260 101 78
NaeI GCC'GGC
Cuts at: 0 763 1188
Size: 763 425
NgoMIV G'CCGG_C
Cuts at: 0 761 1188
Size: 761 427
PfoI T'CCnGG_A
Cuts at: 0 1019 1188
Size: 1019 169
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 124 156 1188
Size: 124 32 1032
Fragments arranged by size:
1032 124 32
PstI C_TGCA'G
Cuts at: 0 31 413 1188
Size: 31 382 775
Fragments arranged by size:
775 382 31
PvuII CAG'CTG
Cuts at: 0 1087 1188
Size: 1087 101
SacII CC_GC'GG
Cuts at: 0 261 339 1188
Size: 261 78 849
Fragments arranged by size:
849 261 78
SfcI C'TryA_G
Cuts at: 0 27 409 1188
Size: 27 382 779
Fragments arranged by size:
779 382 27
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 283 1188
Size: 283 905
SmaI CCC'GGG
Cuts at: 0 165 1188
Size: 165 1023
StyI C'CwwG_G
Cuts at: 0 365 849 1188
Size: 365 484 339
Fragments arranged by size:
484 365 339
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 936 1188
Size: 936 252
TatI w'GTAC_w
Cuts at: 0 572 1188
Size: 572 616
Tth111I GACn'n_nGTC
Cuts at: 0 742 1188
Size: 742 446
XbaI T'CTAG_A
Cuts at: 0 801 1188
Size: 801 387
XmaI C'CCGG_G
Cuts at: 0 163 1188
Size: 163 1025
XmnI GAAnn'nnTTC
Cuts at: 0 403 1188
Size: 403 785
ZraI GAC'GTC
Cuts at: 0 1053 1188
Size: 1053 135
Enzymes that do cut:
AarI AatII AflIII AleI AlwNI AvaI AvrII BaeI
BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI
BglI BmrI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI
BsaXI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI
BsrDI BsrFI BsrGI BstYI BtgI BtsI DrdI EaeI
Eco57MI HaeII Hin4I HincII MluI MmeI MscI MslI
MspA1I NaeI NgoMIV PfoI PsrI PstI PvuII SacII
SfcI SfiI SmaI StyI TaqII TatI Tth111I XbaI
XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AcuI AfeI AflII AgeI AhdI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
BbeI BfrBI BglII BlpI Bme1580I BmgBI BmtI BplI
BpuEI BsaBI BseRI BseYI BsiWI BsmI BspHI BsrBI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII EagI EarI EciI
EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KasI KpnI MfeI NarI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PvuI RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfoI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI XcmI
XhoI