(Circular) (Six-base) MAPSORT of: pFN21ASDA1205.seq Check: 3992 from: 1 to: 8374 pFN21ASDA1205 8374 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2805 2805 Size: 8374 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6184 278 Size: 53 83 186 936 4648 2468 Fragments arranged by size: 4648 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5717 1937 Size: 3780 4594 Acc65I G'GTAC_C Cuts at: 4482 5695 4482 Size: 1213 7161 AclI AA'CG_TT Cuts at: 1563 6501 6874 1563 Size: 4938 373 3063 Fragments arranged by size: 4938 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3245 3689 4985 5270 5608 6437 7821 8089 Size: 1613 444 1296 285 338 829 1384 268 Cuts at: 8089 1632 Size: 1917 Fragments arranged by size: 1917 1613 1384 1296 829 444 338 285 268 AflII C'TTAA_G Cuts at: 820 1017 3233 820 Size: 197 2216 5961 Fragments arranged by size: 5961 2216 197 AflIII A'CryG_T Cuts at: 1203 4802 5292 7273 8250 1203 Size: 3599 490 1981 977 1327 Fragments arranged by size: 3599 1981 1327 977 490 AgeI A'CCGG_T Cuts at: 7205 7205 Size: 8374 AhdI GACnn_n'nnGTC Cuts at: 5026 7103 5026 Size: 2077 6297 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8374 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4452 1184 Size: 3268 5106 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2402 2434 2843 2875 2402 Size: 32 409 32 7901 Fragments arranged by size: 7901 409 32 32 AlwNI CAG_nnn'CTG Cuts at: 3305 4118 5069 5167 5527 5661 6117 7689 3305 Size: 813 951 98 360 134 456 1572 3990 Fragments arranged by size: 3990 1572 951 813 456 360 134 98 ApaI G_GGCC'C Cuts at: 2535 2568 3120 5003 2535 Size: 33 552 1883 5906 Fragments arranged by size: 5906 1883 552 33 ApaLI G'TGCA_C Cuts at: 6431 7587 6431 Size: 1156 7218 ApoI r'AATT_y Cuts at: 1493 4397 5683 5909 6061 8170 1493 Size: 2904 1286 226 152 2109 1697 Fragments arranged by size: 2904 2109 1697 1286 226 152 AseI AT'TA_AT Cuts at: 160 1032 6928 160 Size: 872 5896 1606 Fragments arranged by size: 5896 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8374 AvaI C'yCGr_G Cuts at: 1870 1943 2108 2168 2210 2567 3159 3325 3336 Size: 73 165 60 42 357 592 166 11 Cuts at: 3336 3516 3680 3902 4172 4745 5699 1870 Size: 180 164 222 270 573 954 4545 Fragments arranged by size: 4545 954 592 573 357 270 222 180 166 165 164 73 60 42 11 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2238 2271 2238 Size: 33 8341 BamHI G'GATC_C Cuts at: 2182 2281 3084 5704 6071 2182 Size: 99 803 2620 367 4485 Fragments arranged by size: 4485 2620 803 367 99 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2041 2192 2236 2291 2318 Size: 325 217 627 254 151 44 55 27 Cuts at: 2318 2326 2671 2805 3018 3146 3206 3593 4482 Size: 8 345 134 213 128 60 387 889 Cuts at: 4482 5543 5695 7150 618 Size: 1061 152 1455 1842 Fragments arranged by size: 1842 1455 1061 889 627 387 345 325 254 217 213 152 151 134 128 60 55 44 27 8 BanII G_rGCy'C Cuts at: 1391 2142 2218 2535 2568 3120 3222 3876 3909 Size: 751 76 317 33 552 102 654 33 Cuts at: 3909 4149 4737 5003 5044 5333 5425 5693 1391 Size: 240 588 266 41 289 92 268 4072 Fragments arranged by size: 4072 751 654 588 552 317 289 268 266 240 102 92 76 41 33 33 BbeI G_GCGC'C Cuts at: 2196 2240 2295 2330 2675 2809 3022 3150 3597 Size: 44 55 35 345 134 213 128 447 Cuts at: 3597 5547 2196 Size: 1950 5023 Fragments arranged by size: 5023 1950 447 345 213 134 128 55 44 35 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3720 4173 928 Size: 971 1821 453 5129 Fragments arranged by size: 5129 1821 971 453 BbvCI CC'TCA_GC Cuts at: 3192 3960 3966 4311 3192 Size: 768 6 345 7255 Fragments arranged by size: 7255 768 345 6 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6565 6599 1903 Size: 34 4628 34 3678 Fragments arranged by size: 4628 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2239 5450 6266 7476 2239 Size: 3211 816 1210 3137 Fragments arranged by size: 3211 3137 1210 816 BclI T'GATC_A Cuts at: 1548 1548 Size: 8374 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2316 3064 3602 Size: 107 122 71 631 605 644 748 538 Cuts at: 3602 3839 4127 5007 6985 136 Size: 237 288 880 1978 1525 Fragments arranged by size: 1978 1525 880 748 644 631 605 538 288 237 122 107 71 BglII A'GATC_T Cuts at: 8369 8369 Size: 8374 BlpI GC'TnA_GC Cuts at: 3479 5264 5393 5791 3479 Size: 1785 129 398 6062 Fragments arranged by size: 6062 1785 398 129 Bme1580I G_kGCm'C Cuts at: 1790 2535 2568 3120 3209 3255 5003 6435 7591 Size: 745 33 552 89 46 1748 1432 1156 Cuts at: 7591 1790 Size: 2573 Fragments arranged by size: 2573 1748 1432 1156 745 552 89 46 33 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8374 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2978 3873 4565 Size: 416 53 83 399 24 1561 895 692 Cuts at: 4565 6105 7063 442 Size: 1540 958 1753 Fragments arranged by size: 1753 1561 1540 958 895 692 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8374 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3351 3733 3769 4771 3351 Size: 382 36 1002 6954 Fragments arranged by size: 6954 1002 382 36 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2156 2240 2920 3116 3620 3938 5058 5189 Size: 917 84 680 196 504 318 1120 131 Cuts at: 5189 7034 1239 Size: 1845 2579 Fragments arranged by size: 2579 1845 1120 917 680 504 318 196 131 84 Bpu10I CC'TnA_GC Cuts at: 3192 3960 3966 4311 4731 3192 Size: 768 6 345 420 6835 Fragments arranged by size: 6835 768 420 345 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2762 3410 4313 5387 6499 7364 7662 2762 Size: 648 903 1074 1112 865 298 3474 Fragments arranged by size: 3474 1112 1074 903 865 648 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2938 3729 4664 7037 882 Size: 617 1439 791 935 2373 2219 Fragments arranged by size: 2373 2219 1439 935 791 617 BsaAI yAC'GTr Cuts at: 493 1204 4534 4805 5119 5671 6165 493 Size: 711 3330 271 314 552 494 2702 Fragments arranged by size: 3330 2702 711 552 494 314 271 BsaBI GATnn'nnATC Cuts at: 8368 8368 Size: 8374 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2193 2237 2292 2327 Size: 53 83 186 936 660 44 55 35 Cuts at: 2327 2552 2672 2806 3019 3147 3470 3594 5074 Size: 225 120 134 213 128 323 124 1480 Cuts at: 5074 5544 6181 6563 7198 275 Size: 470 637 382 635 1451 Fragments arranged by size: 1480 1451 936 660 637 635 470 382 323 225 213 186 134 128 124 120 83 55 53 44 35 BsaWI w'CCGG_w Cuts at: 3572 4405 5109 5335 6807 7205 7479 7626 8037 Size: 833 704 226 1472 398 274 147 411 Cuts at: 8037 3572 Size: 3909 Fragments arranged by size: 3909 1472 833 704 411 398 274 226 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2402 2432 2843 2873 2918 Size: 30 165 30 999 30 411 30 45 Cuts at: 2918 2948 3377 3407 3928 3958 1178 Size: 30 429 30 521 30 5594 Fragments arranged by size: 5594 999 521 429 411 165 45 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2186 2399 2426 3209 3386 3677 3691 3745 Size: 1000 213 27 783 177 291 14 54 Cuts at: 3745 3935 3948 4125 4157 4262 5005 5422 5579 Size: 190 13 177 32 105 743 417 157 Cuts at: 5579 1186 Size: 3981 Fragments arranged by size: 3981 1000 783 743 417 291 213 190 177 177 157 105 54 32 27 14 13 BseYI C'CCAG_C Cuts at: 2150 2306 2492 2638 3531 3791 4037 4616 4648 Size: 156 186 146 893 260 246 579 32 Cuts at: 4648 5045 5269 5366 7577 8073 2150 Size: 397 224 97 2211 496 2451 Fragments arranged by size: 2451 2211 893 579 496 397 260 246 224 186 156 146 97 32 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4614 5273 1163 Size: 573 2878 659 4264 Fragments arranged by size: 4264 2878 659 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2072 5824 6585 7613 1540 Size: 275 186 71 3752 761 1028 2301 Fragments arranged by size: 3752 2301 1028 761 275 186 71 BsiHKAI G_wGCw'C Cuts at: 4549 5138 5235 6435 6520 7591 4549 Size: 589 97 1200 85 1071 5332 Fragments arranged by size: 5332 1200 1071 589 97 85 BsmI GAATG_Cn' Cuts at: 5885 5978 5885 Size: 93 8281 BsmBI CGTCTCn'nnnn_ Cuts at: 3677 4164 4211 4232 4712 3677 Size: 487 47 21 480 7339 Fragments arranged by size: 7339 487 480 47 21 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2142 2218 2535 2568 3120 3209 3222 Size: 399 352 76 317 33 552 89 13 Cuts at: 3222 3255 3876 3909 4149 4549 4737 5003 5044 Size: 33 621 33 240 400 188 266 41 Cuts at: 5044 5138 5235 5333 5425 5693 6435 6520 7591 Size: 94 97 98 92 268 742 85 1071 Cuts at: 7591 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 621 552 400 399 352 317 268 266 240 188 98 97 94 92 89 85 76 41 33 33 33 13 BspEI T'CCGG_A Cuts at: 3572 4405 5335 8037 3572 Size: 833 930 2702 3909 Fragments arranged by size: 3909 2702 930 833 BspHI T'CATG_A Cuts at: 6263 7993 6263 Size: 1730 6644 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2805 3747 4894 5202 5640 5729 844 Size: 1961 942 1147 308 438 89 3489 Fragments arranged by size: 3489 1961 1147 942 438 308 89 BsrBI CCG'CTC Cuts at: 1394 1823 2344 2946 3478 4813 4831 4864 5059 Size: 429 521 602 532 1335 18 33 195 Cuts at: 5059 6261 1394 Size: 1202 3507 Fragments arranged by size: 3507 1335 1202 602 532 521 429 195 33 18 BsrDI GCAATG_nn' Cuts at: 65 1248 3898 4817 6869 7043 65 Size: 1183 2650 919 2052 174 1396 Fragments arranged by size: 2650 2052 1396 1183 919 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2710 4253 7018 7205 1700 Size: 110 900 1543 2765 187 2869 Fragments arranged by size: 2869 2765 1543 900 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8374 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8374 BssSI C'ACGA_G Cuts at: 6434 7446 6434 Size: 1012 7362 BstAPI GCAn_nnn'nTGC Cuts at: 794 2206 5584 794 Size: 1412 3378 3584 Fragments arranged by size: 3584 3378 1412 BstBI TT'CG_AA Cuts at: 2007 7178 2007 Size: 5171 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8374 BstXI CCAn_nnnn'nTGG Cuts at: 4368 4368 Size: 8374 BstYI r'GATC_y Cuts at: 1972 2182 2281 3084 5704 6071 6456 6473 7925 Size: 210 99 803 2620 367 385 17 1452 Cuts at: 7925 8011 8023 8369 1972 Size: 86 12 346 1977 Fragments arranged by size: 2620 1977 1452 803 385 367 346 210 99 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2463 4140 4641 2463 Size: 1677 501 6196 Fragments arranged by size: 6196 1677 501 BtgI C'CryG_G Cuts at: 513 1065 2022 2128 2142 2406 2433 2619 3022 Size: 552 957 106 14 264 27 186 403 Cuts at: 3022 3068 3837 4875 5597 513 Size: 46 769 1038 722 3290 Fragments arranged by size: 3290 1038 957 769 722 552 403 264 186 106 46 27 14 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5108 487 Size: 683 56 3882 3753 Fragments arranged by size: 3882 3753 683 56 BtsI GCAGTG_nn' Cuts at: 3108 4233 5585 5890 6684 6704 3108 Size: 1125 1352 305 794 20 4778 Fragments arranged by size: 4778 1352 1125 794 305 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8374 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8339 DraI TTT'AAA Cuts at: 5678 5678 Size: 8374 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4298 7381 809 Size: 507 2982 3083 1802 Fragments arranged by size: 3083 2982 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2545 3025 3065 Size: 54 1425 50 275 18 715 480 40 Cuts at: 3065 4203 4420 4722 4814 4856 5529 5821 6710 Size: 1138 217 302 92 42 673 292 889 Cuts at: 6710 8339 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1138 889 715 673 480 302 292 275 217 92 54 50 43 42 40 18 EagI C'GGCC_G Cuts at: 1537 1812 5821 1537 Size: 275 4009 4090 Fragments arranged by size: 4090 4009 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3870 4943 5334 6304 1353 Size: 2517 1073 391 970 3423 Fragments arranged by size: 3423 2517 1073 970 391 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2175 2679 2882 2891 3077 3748 Size: 1064 138 586 504 203 9 186 671 Cuts at: 3748 3963 3975 4281 6961 7335 7481 387 Size: 215 12 306 2680 374 146 1280 Fragments arranged by size: 2680 1280 1064 671 586 504 374 306 215 203 186 146 138 12 9 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 2240 2920 3116 3245 3620 3689 Size: 393 524 84 680 196 129 375 69 Cuts at: 3689 3938 4985 5058 5189 5270 5608 6437 7034 Size: 249 1047 73 131 81 338 829 597 Cuts at: 7034 7821 8089 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1047 829 787 680 597 524 393 375 338 268 249 196 131 129 84 81 73 69 EcoNI CCTnn'n_nnAGG Cuts at: 5558 5558 Size: 8374 EcoO109I rG'GnC_Cy Cuts at: 2051 2476 2532 3002 3078 3100 3116 4384 4645 Size: 425 56 470 76 22 16 1268 261 Cuts at: 4645 4999 5164 5551 2051 Size: 354 165 387 4874 Fragments arranged by size: 4874 1268 470 425 387 354 261 165 76 56 22 16 EcoRI G'AATT_C Cuts at: 5683 6061 5683 Size: 378 7996 FspI TGC'GCA Cuts at: 6880 6880 Size: 8374 HaeII r_GCGC'y Cuts at: 1678 2196 2240 2295 2330 2493 2675 2809 2920 Size: 518 44 55 35 163 182 134 111 Cuts at: 2920 3022 3150 3442 3597 4492 5547 7521 1678 Size: 102 128 292 155 895 1055 1974 2531 Fragments arranged by size: 2531 1974 1055 895 518 292 182 163 155 134 128 111 102 55 44 35 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2167 2199 2918 2950 3281 Size: 32 135 32 595 32 719 32 331 Cuts at: 3281 3313 3377 3409 3604 3636 4260 4292 5012 Size: 32 64 32 195 32 624 32 720 Cuts at: 5012 5044 7015 7047 7089 7121 1373 Size: 32 1971 32 42 32 2626 Fragments arranged by size: 2626 1971 720 719 624 595 331 195 135 64 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2626 5718 5964 669 Size: 988 281 688 3092 246 3079 Fragments arranged by size: 3092 3079 988 688 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8374 HpaI GTT'AAC Cuts at: 5964 5964 Size: 8374 KasI G'GCGC_C Cuts at: 2192 2236 2291 2326 2671 2805 3018 3146 3593 Size: 44 55 35 345 134 213 128 447 Cuts at: 3593 5543 2192 Size: 1950 5023 Fragments arranged by size: 5023 1950 447 345 213 134 128 55 44 35 KpnI G_GTAC'C Cuts at: 4486 5699 4486 Size: 1213 7161 MfeI C'AATT_G Cuts at: 5973 5973 Size: 8374 MluI A'CGCG_T Cuts at: 8250 8250 Size: 8374 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3195 7488 7672 1305 Size: 1890 4293 184 2007 Fragments arranged by size: 4293 2007 1890 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4205 5531 10 Size: 54 1425 343 2373 1326 2853 Fragments arranged by size: 2853 2373 1425 1326 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 6332 6691 6850 518 Size: 648 5166 359 159 2042 Fragments arranged by size: 5166 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2032 2144 2227 2435 2638 2940 3839 3913 Size: 426 112 83 208 203 302 899 74 Cuts at: 3913 4021 4060 4118 4842 5305 5661 5790 6467 Size: 108 39 58 724 463 356 129 677 Cuts at: 6467 7615 7860 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 899 724 677 463 426 356 302 245 208 203 129 112 108 83 74 58 39 NaeI GCC'GGC Cuts at: 2712 4255 2712 Size: 1543 6831 NarI GG'CG_CC Cuts at: 2193 2237 2292 2327 2672 2806 3019 3147 3594 Size: 44 55 35 345 134 213 128 447 Cuts at: 3594 5544 2193 Size: 1950 5023 Fragments arranged by size: 5023 1950 447 345 213 134 128 55 44 35 NcoI C'CATG_G Cuts at: 513 1065 2022 2128 2406 513 Size: 552 957 106 278 6481 Fragments arranged by size: 6481 957 552 278 106 NdeI CA'TA_TG Cuts at: 387 387 Size: 8374 NgoMIV G'CCGG_C Cuts at: 2710 4253 2710 Size: 1543 6831 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8374 NotI GC'GGCC_GC Cuts at: 5821 5821 Size: 8374 NspI r_CATG'y Cuts at: 1132 1229 5587 5732 7277 1132 Size: 97 4358 145 1545 2229 Fragments arranged by size: 4358 2229 1545 145 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8374 PciI A'CATG_T Cuts at: 7273 7273 Size: 8374 PflMI CCAn_nnn'nTGG Cuts at: 1108 2134 5167 5527 1108 Size: 1026 3033 360 3955 Fragments arranged by size: 3955 3033 1026 360 PfoI T'CCnGG_A Cuts at: 4281 4506 4281 Size: 225 8149 PmeI GTTT'AAAC Cuts at: 5678 5678 Size: 8374 PmlI CAC'GTG Cuts at: 4805 4805 Size: 8374 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2402 2434 2843 2875 6443 6475 7956 7988 2402 Size: 32 409 32 3568 32 1481 32 2788 Fragments arranged by size: 3568 2788 1481 409 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2476 3002 3100 5551 2476 Size: 526 98 2451 5299 Fragments arranged by size: 5299 2451 526 98 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8374 PsiI TTA'TAA Cuts at: 5944 5944 Size: 8374 PspOMI G'GGCC_C Cuts at: 2531 2564 3116 4999 2531 Size: 33 552 1883 5906 Fragments arranged by size: 5906 1883 552 33 PspXI vC'TCGA_Gb Cuts at: 2108 3325 2108 Size: 1217 7157 PstI C_TGCA'G Cuts at: 830 2106 3528 3610 3744 3847 4395 5280 5726 Size: 1276 1422 82 134 103 548 885 446 Cuts at: 5726 830 Size: 3478 Fragments arranged by size: 3478 1422 1276 885 548 446 134 103 82 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8374 PvuII CAG'CTG Cuts at: 2032 2940 5305 2032 Size: 908 2365 5101 Fragments arranged by size: 5101 2365 908 SacII CC_GC'GG Cuts at: 2145 2436 3840 2145 Size: 291 1404 6679 Fragments arranged by size: 6679 1404 291 SalI G'TCGA_C Cuts at: 1936 5716 1936 Size: 3780 4594 SanDI GG'GwC_CC Cuts at: 2476 2476 Size: 8374 SbfI CC_TGCA'GG Cuts at: 3744 5280 5726 3744 Size: 1536 446 6392 Fragments arranged by size: 6392 1536 446 ScaI AGT'ACT Cuts at: 6622 8334 6622 Size: 1712 6662 SexAI A'CCwGG_T Cuts at: 2928 2928 Size: 8374 SfcI C'TryA_G Cuts at: 826 1045 2102 3524 3606 3740 3843 4391 4986 Size: 219 1057 1422 82 134 103 548 595 Cuts at: 4986 5276 5722 6857 7538 7729 826 Size: 290 446 1135 681 191 1471 Fragments arranged by size: 1471 1422 1135 1057 681 595 548 446 290 219 191 134 103 82 SfiI GGCCn_nnn'nGGCC Cuts at: 3064 3064 Size: 8374 SfoI GGC'GCC Cuts at: 2194 2238 2293 2328 2673 2807 3020 3148 3595 Size: 44 55 35 345 134 213 128 447 Cuts at: 3595 5545 2194 Size: 1950 5023 Fragments arranged by size: 5023 1950 447 345 213 134 128 55 44 35 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8374 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8374 SmaI CCC'GGG Cuts at: 1872 3161 5701 1872 Size: 1289 2540 4545 Fragments arranged by size: 4545 2540 1289 SmlI C'TyrA_G Cuts at: 820 1017 1943 2108 2777 3233 3325 3425 3680 Size: 197 926 165 669 456 92 100 255 Cuts at: 3680 4328 5402 6478 7379 7641 820 Size: 648 1074 1076 901 262 1553 Fragments arranged by size: 1553 1076 1074 926 901 669 648 456 262 255 197 165 100 92 SnaBI TAC'GTA Cuts at: 493 5671 6165 493 Size: 5178 494 2702 Fragments arranged by size: 5178 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8374 SphI G_CATG'C Cuts at: 1132 5587 5732 1132 Size: 4455 145 3774 Fragments arranged by size: 4455 3774 145 SspI AAT'ATT Cuts at: 5 52 6298 5 Size: 47 6246 2081 Fragments arranged by size: 6246 2081 47 StuI AGG'CCT Cuts at: 1517 3522 1517 Size: 2005 6369 StyI C'CwwG_G Cuts at: 513 1065 2022 2128 2322 2406 2481 2724 3255 Size: 552 957 106 194 84 75 243 531 Cuts at: 3255 3828 4920 5813 513 Size: 573 1092 893 3074 Fragments arranged by size: 3074 1092 957 893 573 552 531 243 194 106 84 75 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2107 3007 3459 3949 4378 6401 6418 Size: 1255 527 900 452 490 429 2023 17 Cuts at: 6418 6571 6756 7201 325 Size: 153 185 445 1498 Fragments arranged by size: 2023 1498 1255 900 527 490 452 445 429 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6620 8332 96 Size: 274 80 33 51 1443 4643 1712 138 Fragments arranged by size: 4643 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3928 3960 8334 8366 3928 Size: 32 4374 32 3936 Fragments arranged by size: 4374 3936 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2930 3719 1316 Size: 1614 789 5971 Fragments arranged by size: 5971 1614 789 XbaI T'CTAG_A Cuts at: 5710 5710 Size: 8374 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4450 4450 Size: 8374 XhoI C'TCGA_G Cuts at: 1943 2108 3325 3680 1943 Size: 165 1217 355 6637 Fragments arranged by size: 6637 1217 355 165 XmaI C'CCGG_G Cuts at: 1870 3159 5699 1870 Size: 1289 2540 4545 Fragments arranged by size: 4545 2540 1289 XmnI GAAnn'nnTTC Cuts at: 6503 6503 Size: 8374 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6182 276 Size: 53 83 186 936 4648 2468 Fragments arranged by size: 4648 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BfrBI BsiWI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PsrI RsrII SacI SapI SrfI SwaI