Restriction map for
pFN21ASDA1201
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AccI AccIII AcyI
AflII AgeI AlwNI AocI Aor51HI
AscI AseI AsnI Asp718I AspI
AviII AvrII BamHI BanI BbeI
BbrPI BbvCI BcgI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaHI BseAI BsiWI BspDI
BspEI BspHI BsrDI BssHII BssSI
Bst1107I BstBI BstZ17I Bsu36I CelII
ClaI CpoI DraI DrdI Eco47III
Eco81I EcoT22I FbaI FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MamI
MfeI MluI MroI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tth111I Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AclI 1419 AACGTT AA^CGTT
AcsI 105 GAATTC [GA]^AATT[CT]
Alw44I 230 GTGCAC G^TGCAC
ApaLI 230 GTGCAC G^TGCAC
ApoI 105 GAATTC [GA]^AATT[CT]
AvaI 443 CCCGGG C^[CT]CG[GA]G
BalI 1484 TGGCCA TGG^CCA
BciVI 616 GGATAC GTATCC(6/5)
BglI 89 GCCGGAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 48 ACCGGA [AT]^CCGG[AT]
BsgI 61 GTGCAG GTGCAG(16/14)
BsiEI 87 CGGCCG CG[GA][CT]^CG
BsmI 483 GAATGC GAATGC(1/-1)
BsoBI 443 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1232 TGTACA T^GTACA
BspLU11I 1668 ACATGT A^CATGT
BsrBI 93 GAGCGG CCGCTC(-3/-3)
BsrFI 998 GCCGGC [GA]^CCGG[CT]
BsrGI 1232 TGTACA T^GTACA
BstAPI 54 GCAGGGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 998 GCCGGC [GA]^CCGG[CT]
DraIII 1288 CACGACGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1123 CCATGG C^C[GA][CT]GG
EagI 87 CGGCCG C^GGCCG
EclXI 87 CGGCCG C^GGCCG
Eco52I 87 CGGCCG C^GGCCG
EcoRI 105 GAATTC G^AATTC
EcoRV 1108 GATATC GAT^ATC
MluNI 1484 TGGCCA TGG^CCA
MscI 1484 TGGCCA TGG^CCA
MslI 1338 CACCCGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 998 GCCGGC GCC^GGC
NcoI 1123 CCATGG C^CATGG
NgoMIV 998 GCCGGC G^CCGGC
NspI 1668 ACATGT [GA]CATG^[CT]
Psp1406I 1419 AACGTT AA^CGTT
PstI 1729 CTGCAG CTGCA^G
SapI 1812 GCTCTTC GCTCTTC(1/4)
SmaI 443 CCCGGG CCC^GGG
SspBI 1232 TGTACA T^GTACA
StuI 974 AGGCCT AGG^CCT
XcmI 1742 CCAAGCTGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 443 CCCGGG C^CCGGG
XmaI 443 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 1691 CGCG CG^CG
2050 CGCG
AflIII 1668 ACATGT A^C[GA][CT]GT
2057 ACACGT
AhdI 1364 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2053 GACTACACGTC
ApaI 440 GGGCCC GGGCC^C
1913 GGGCCC
Asp700I 1416 GAAAACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1431 GAAGAACTTC
AspEI 1364 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2053 GACTACACGTC
BbsI 166 GAAGAC GAAGAC(2/6)
1018 GTCTTC
BpuAI 166 GAAGAC GAAGAC(2/6)
1018 GTCTTC
BsaI 682 GAGACC GGTCTC(1/5)
1371 GGTCTC
BsmBI 768 CGTCTC CGTCTC(1/5)
812 GAGACG
BstEII 1626 GGTCACC G^GT[ACGT]ACC
1889 GGTTACC
BstPI 1626 GGTCACC G^GT[ACGT]ACC
1889 GGTTACC
BstUI 1691 CGCG CG^CG
2050 CGCG
BstXI 537 CCAGTGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1184 CCAACCCTCTGG
BstYI 284 GGATCT [GA]^GATC[CT]
629 AGATCC
Eam1105I 1364 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2053 GACTACACGTC
EcoNI 822 CCTGGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1605 CCTGGAAGAGG
EcoO65I 1626 GGTCACC G^GT[ACGT]ACC
1889 GGTTACC
EcoT14I 512 CCAAGG C^C[AT][AT]GG
1123 CCATGG
FauI 2048 CCCGC CCCGC(4/6)
1077 GCGGG
HgaI 1689 GACGC GACGC(5/10)
1575 GCGTC
MflI 284 GGATCT [GA]^GATC[CT]
629 AGATCC
MvnI 1691 CGCG CG^CG
2050 CGCG
SfcI 294 CTACAG C^T[GA][CT]AG
1729 CTGCAG
StyI 512 CCAAGG C^C[AT][AT]GG
1123 CCATGG
TaqI 1392 TCGA T^CGA
1427 TCGA
Tsp45I 938 GTGAC ^GT[GC]AC
1627 GTCAC
TthHB8I 1392 TCGA T^CGA
1427 TCGA
XhoII 284 GGATCT [GA]^GATC[CT]
629 AGATCC
XmnI 1416 GAAAACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1431 GAAGAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BanII BbvI BcnI BmyI
BpmI BsaAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MnlI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
TfiI Tru9I TseI Tsp509I TspRI