(Circular) (Six-base) MAPSORT of: pFN21ASDA1200.seq Check: 7973 from: 1 to: 8821 pFN21ASDA1200 8821 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2488 4397 2488 Size: 1909 6912 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6631 278 Size: 53 83 186 936 5095 2468 Fragments arranged by size: 5095 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2058 3414 3438 6164 1937 Size: 121 1356 24 2726 4594 Fragments arranged by size: 4594 2726 1356 121 24 Acc65I G'GTAC_C Cuts at: 6142 6142 Size: 8821 AclI AA'CG_TT Cuts at: 1563 5846 6107 6948 7321 1563 Size: 4283 261 841 373 3063 Fragments arranged by size: 4283 3063 841 373 261 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2270 2583 2675 2733 2830 3470 4055 4996 Size: 638 313 92 58 97 640 585 941 Cuts at: 4996 5820 6884 8268 8536 1632 Size: 824 1064 1384 268 1917 Fragments arranged by size: 1917 1384 1064 941 824 640 638 585 313 268 97 92 58 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8624 AflIII A'CryG_T Cuts at: 1203 5278 7720 8697 1203 Size: 4075 2442 977 1327 Fragments arranged by size: 4075 2442 1327 977 AgeI A'CCGG_T Cuts at: 7652 7652 Size: 8821 AhdI GACnn_n'nnGTC Cuts at: 7550 7550 Size: 8821 AleI CACnn'nnGTG Cuts at: 1166 4313 1166 Size: 3147 5674 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5039 1184 Size: 3855 4966 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5340 5372 5340 Size: 32 8789 AlwNI CAG_nnn'CTG Cuts at: 2158 2170 2467 2488 2653 3692 4030 4403 5098 Size: 12 297 21 165 1039 338 373 695 Cuts at: 5098 5489 6564 8136 2158 Size: 391 1075 1572 2843 Fragments arranged by size: 2843 1572 1075 1039 695 391 373 338 297 165 21 12 ApaI G_GGCC'C Cuts at: 5575 5575 Size: 8821 ApaLI G'TGCA_C Cuts at: 5024 5813 6878 8034 5024 Size: 789 1065 1156 5811 Fragments arranged by size: 5811 1156 1065 789 ApoI r'AATT_y Cuts at: 1493 6130 6356 6508 8617 1493 Size: 4637 226 152 2109 1697 Fragments arranged by size: 4637 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 7375 160 Size: 872 6343 1606 Fragments arranged by size: 6343 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8821 AvaI C'yCGr_G Cuts at: 1870 1943 3120 3132 3591 3859 4694 5164 6146 Size: 73 1177 12 459 268 835 470 982 Cuts at: 6146 1870 Size: 4545 Fragments arranged by size: 4545 1177 982 835 470 459 268 73 12 BamHI G'GATC_C Cuts at: 2504 4011 6151 6518 2504 Size: 1507 2140 367 4807 Fragments arranged by size: 4807 2140 1507 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2044 2560 2684 2978 3012 Size: 325 217 627 257 516 124 294 34 Cuts at: 3012 4076 4358 4500 5592 6142 7597 618 Size: 1064 282 142 1092 550 1455 1842 Fragments arranged by size: 1842 1455 1092 1064 627 550 516 325 294 282 257 217 142 124 34 BanII G_rGCy'C Cuts at: 1391 2328 2813 2974 3203 3632 5575 5906 6140 Size: 937 485 161 229 429 1943 331 234 Cuts at: 6140 1391 Size: 4072 Fragments arranged by size: 4072 1943 937 485 429 331 234 229 161 BbeI G_GCGC'C Cuts at: 2048 2048 Size: 8821 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3575 3806 5727 928 Size: 971 1676 231 1921 4022 Fragments arranged by size: 4022 1921 1676 971 231 BbvCI CC'TCA_GC Cuts at: 4815 5588 4815 Size: 773 8048 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7012 7046 1903 Size: 34 5075 34 3678 Fragments arranged by size: 5075 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4369 6713 7923 4369 Size: 2344 1210 5267 Fragments arranged by size: 5267 2344 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 8821 BfrBI ATG'CAT Cuts at: 2829 2829 Size: 8821 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5441 5493 7432 Size: 107 122 71 631 605 3769 52 1939 Cuts at: 7432 136 Size: 1525 Fragments arranged by size: 3769 1939 1525 631 605 122 107 71 52 BglII A'GATC_T Cuts at: 4710 5205 5885 8816 4710 Size: 495 680 2931 4715 Fragments arranged by size: 4715 2931 680 495 BlpI GC'TnA_GC Cuts at: 2346 3219 5514 5718 6238 2346 Size: 873 2295 204 520 4929 Fragments arranged by size: 4929 2295 873 520 204 Bme1580I G_kGCm'C Cuts at: 1790 2565 2689 3045 4433 5028 5575 5817 6882 Size: 775 124 356 1388 595 547 242 1065 Cuts at: 6882 8038 1790 Size: 1156 2573 Fragments arranged by size: 2573 1388 1156 1065 775 595 547 356 242 124 BmgBI CAC'GTC Cuts at: 1318 3804 1318 Size: 2486 6335 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 6552 7510 442 Size: 416 53 83 399 24 5135 958 1753 Fragments arranged by size: 5135 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8821 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2918 3097 2918 Size: 179 8642 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2180 2195 2372 3134 3245 3779 4007 4022 Size: 941 15 177 762 111 534 228 15 Cuts at: 4022 4041 4296 4516 5075 5198 5510 5511 7481 Size: 19 255 220 559 123 312 1 1970 Cuts at: 7481 1239 Size: 2579 Fragments arranged by size: 2579 1970 941 762 559 534 312 255 228 220 177 123 111 19 15 15 1 Bpu10I CC'TnA_GC Cuts at: 3108 3693 4815 5588 3108 Size: 585 1122 773 6341 Fragments arranged by size: 6341 1122 773 585 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2018 2417 2468 3867 4568 5444 6946 7811 8109 Size: 399 51 1399 701 876 1502 865 298 Cuts at: 8109 2018 Size: 2730 Fragments arranged by size: 2730 1502 1399 876 865 701 399 298 51 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2198 7484 882 Size: 617 699 5286 2219 Fragments arranged by size: 5286 2219 699 617 BsaAI yAC'GTr Cuts at: 493 1204 4380 6118 6612 493 Size: 711 3176 1738 494 2702 Fragments arranged by size: 3176 2702 1738 711 494 BsaBI GATnn'nnATC Cuts at: 8815 8815 Size: 8821 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2045 3609 3660 5781 Size: 53 83 186 936 512 1564 51 2121 Cuts at: 5781 6628 7010 7645 275 Size: 847 382 635 1451 Fragments arranged by size: 2121 1564 1451 936 847 635 512 382 186 83 53 51 BsaWI w'CCGG_w Cuts at: 2100 2280 3873 5937 7254 7652 7926 8073 8484 Size: 180 1593 2064 1317 398 274 147 411 Cuts at: 8484 2100 Size: 2437 Fragments arranged by size: 2437 2064 1593 1317 411 398 274 180 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2621 2651 4039 4069 5340 Size: 30 165 30 1218 30 1388 30 1271 Cuts at: 5340 5370 1178 Size: 30 4629 Fragments arranged by size: 4629 1388 1271 1218 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3176 3544 4019 4340 4775 1186 Size: 1990 368 475 321 435 5232 Fragments arranged by size: 5232 1990 475 435 368 321 BseYI C'CCAG_C Cuts at: 3172 3238 3736 3992 5727 8024 8520 3172 Size: 66 498 256 1735 2297 496 3473 Fragments arranged by size: 3473 2297 1735 498 496 256 66 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2261 3251 4061 4291 5234 1163 Size: 573 525 990 810 230 943 4750 Fragments arranged by size: 4750 990 943 810 573 525 230 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3984 6271 7032 8060 1540 Size: 275 186 1983 2287 761 1028 2301 Fragments arranged by size: 2301 2287 1983 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2328 3632 3699 4335 4801 5028 5184 5817 5906 Size: 1304 67 636 466 227 156 633 89 Cuts at: 5906 6882 6967 8038 2328 Size: 976 85 1071 3111 Fragments arranged by size: 3111 1304 1071 976 636 633 466 227 156 89 85 67 BsmI GAATG_Cn' Cuts at: 6332 6425 6332 Size: 93 8728 BsmBI CGTCTCn'nnnn_ Cuts at: 3616 5330 3616 Size: 1714 7107 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2328 2565 2689 2813 2974 3045 3203 Size: 399 538 237 124 124 161 71 158 Cuts at: 3203 3632 3699 4335 4433 4801 5028 5184 5575 Size: 429 67 636 98 368 227 156 391 Cuts at: 5575 5817 5906 6140 6882 6967 8038 1391 Size: 242 89 234 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 636 538 429 399 391 368 242 237 234 227 161 158 156 124 124 98 89 85 71 67 BspEI T'CCGG_A Cuts at: 3873 5937 8484 3873 Size: 2064 2547 4210 Fragments arranged by size: 4210 2547 2064 BspHI T'CATG_A Cuts at: 4725 6710 8440 4725 Size: 1985 1730 5106 Fragments arranged by size: 5106 1985 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2488 3783 4397 4666 5521 6176 844 Size: 1644 1295 614 269 855 655 3489 Fragments arranged by size: 3489 1644 1295 855 655 614 269 BsrBI CCG'CTC Cuts at: 1394 1823 4492 4918 4939 6708 1394 Size: 429 2669 426 21 1769 3507 Fragments arranged by size: 3507 2669 1769 429 426 21 BsrDI GCAATG_nn' Cuts at: 65 1248 5627 5995 7316 7490 65 Size: 1183 4379 368 1321 174 1396 Fragments arranged by size: 4379 1396 1321 1183 368 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2047 4621 7465 7652 1700 Size: 110 237 2574 2844 187 2869 Fragments arranged by size: 2869 2844 2574 237 187 110 BsrGI T'GTAC_A Cuts at: 96 5295 96 Size: 5199 3622 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8821 BssSI C'ACGA_G Cuts at: 6881 7893 6881 Size: 1012 7809 BstAPI GCAn_nnn'nTGC Cuts at: 794 2170 3703 4507 5188 794 Size: 1376 1533 804 681 4427 Fragments arranged by size: 4427 1533 1376 804 681 BstBI TT'CG_AA Cuts at: 2007 7625 2007 Size: 5618 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8821 BstXI CCAn_nnnn'nTGG Cuts at: 2633 3098 4000 5054 2633 Size: 465 902 1054 6400 Fragments arranged by size: 6400 1054 902 465 BstYI r'GATC_y Cuts at: 1972 2504 3294 3895 4011 4477 4710 4864 5205 Size: 532 790 601 116 466 233 154 341 Cuts at: 5205 5885 6151 6518 6903 6920 8372 8458 8470 Size: 680 266 367 385 17 1452 86 12 Cuts at: 8470 8816 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 790 680 601 532 466 385 367 346 341 266 233 154 116 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2984 3037 5073 5673 513 Size: 552 957 962 53 2036 600 3661 Fragments arranged by size: 3661 2036 962 957 600 552 53 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4938 487 Size: 683 56 3712 4370 Fragments arranged by size: 4370 3712 683 56 BtsI GCAGTG_nn' Cuts at: 4303 4423 4431 4670 4778 4917 5182 6337 7131 Size: 120 8 239 108 139 265 1155 794 Cuts at: 7131 7151 4303 Size: 20 5973 Fragments arranged by size: 5973 1155 794 265 239 139 120 108 20 8 ClaI AT'CG_AT Cuts at: 1554 4243 1554 Size: 2689 6132 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8786 DraI TTT'AAA Cuts at: 6125 6125 Size: 8821 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7828 809 Size: 507 6512 1802 Fragments arranged by size: 6512 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2049 3003 3049 Size: 54 1425 50 275 18 219 954 46 Cuts at: 3049 3306 3981 4120 4882 5676 5979 6268 7157 Size: 257 675 139 762 794 303 289 889 Cuts at: 7157 8786 8 Size: 1629 43 Fragments arranged by size: 1629 1425 954 889 794 762 675 303 289 275 257 219 139 54 50 46 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3981 6268 1537 Size: 275 2169 2287 4090 Fragments arranged by size: 4090 2287 2169 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2220 2797 3803 4595 4613 5074 5153 5839 Size: 867 577 1006 792 18 461 79 686 Cuts at: 5839 6751 1353 Size: 912 3423 Fragments arranged by size: 3423 1006 912 867 792 686 577 461 79 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2096 7408 7782 7928 387 Size: 1064 138 507 5312 374 146 1280 Fragments arranged by size: 5312 1280 1064 507 374 146 138 EcoICRI GAG'CTC Cuts at: 2326 3630 5904 2326 Size: 1304 2274 5243 Fragments arranged by size: 5243 2274 1304 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2180 2195 2270 2372 2583 2675 2733 Size: 393 548 15 75 102 211 92 58 Cuts at: 2733 2830 3134 3245 3470 3779 4007 4022 4041 Size: 97 304 111 225 309 228 15 19 Cuts at: 4041 4055 4296 4516 4996 5075 5198 5510 5511 Size: 14 241 220 480 79 123 312 1 Cuts at: 5511 5820 6884 7481 8268 8536 1239 Size: 309 1064 597 787 268 1524 Fragments arranged by size: 1524 1064 787 597 548 480 393 312 309 309 304 268 241 228 225 220 211 123 111 102 97 92 79 75 58 19 15 15 14 1 EcoNI CCTnn'n_nnAGG Cuts at: 2679 2923 2960 3830 4001 4034 5167 2679 Size: 244 37 870 171 33 1133 6333 Fragments arranged by size: 6333 1133 870 244 171 37 33 EcoO109I rG'GnC_Cy Cuts at: 3129 3208 4579 5173 5486 5533 5571 5572 3129 Size: 79 1371 594 313 47 38 1 6378 Fragments arranged by size: 6378 1371 594 313 79 47 38 1 EcoRI G'AATT_C Cuts at: 6130 6508 6130 Size: 378 8443 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5950 5950 Size: 8821 FspI TGC'GCA Cuts at: 4224 7327 4224 Size: 3103 5718 FspAI rTGC'GCAy Cuts at: 4224 4224 Size: 8821 HaeII r_GCGC'y Cuts at: 1678 2048 2488 3817 5560 7968 1678 Size: 370 440 1329 1743 2408 2531 Fragments arranged by size: 2531 2408 1743 1329 440 370 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2443 2475 2621 2653 3101 Size: 32 135 32 871 32 146 32 448 Cuts at: 3101 3133 3482 3506 3514 3538 3650 3682 3887 Size: 32 349 24 8 24 112 32 205 Cuts at: 3887 3919 5147 5179 5204 5236 5888 5920 5932 Size: 32 1228 32 25 32 652 32 12 Cuts at: 5932 5964 7462 7494 7536 7568 1373 Size: 32 1498 32 42 32 2626 Fragments arranged by size: 2626 1498 1228 871 652 448 349 205 146 135 112 42 32 32 32 32 32 32 32 32 32 32 32 32 32 25 24 24 12 8 HincII GTy'rAC Cuts at: 669 1657 1938 5610 6165 6411 669 Size: 988 281 3672 555 246 3079 Fragments arranged by size: 3672 3079 988 555 281 246 HindIII A'AGCT_T Cuts at: 748 2444 2834 748 Size: 1696 390 6735 Fragments arranged by size: 6735 1696 390 HpaI GTT'AAC Cuts at: 6411 6411 Size: 8821 KasI G'GCGC_C Cuts at: 2044 2044 Size: 8821 KpnI G_GTAC'C Cuts at: 6146 6146 Size: 8821 MfeI C'AATT_G Cuts at: 6420 6420 Size: 8821 MluI A'CGCG_T Cuts at: 5278 8697 5278 Size: 3419 5402 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4876 7935 8119 1305 Size: 3571 3059 184 2007 Fragments arranged by size: 3571 3059 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3005 3308 4122 4884 5678 Size: 54 1425 343 1173 303 814 762 794 Cuts at: 5678 5981 10 Size: 303 2850 Fragments arranged by size: 2850 1425 1173 814 794 762 343 303 303 54 MslI CAynn'nnrTG Cuts at: 518 1166 4313 4616 4964 5021 5876 6779 7138 Size: 648 3147 303 348 57 855 903 359 Cuts at: 7138 7297 518 Size: 159 2042 Fragments arranged by size: 3147 2042 903 855 648 359 348 303 159 57 MspA1I CmG'CkG Cuts at: 1606 2350 2663 2986 3265 3576 3909 4740 4906 Size: 744 313 323 279 311 333 831 166 Cuts at: 4906 5095 5579 5587 5731 6046 6237 6914 8062 Size: 189 484 8 144 315 191 677 1148 Cuts at: 8062 8307 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 831 744 677 484 333 323 315 313 311 279 245 191 189 166 144 8 NaeI GCC'GGC Cuts at: 2049 2049 Size: 8821 NarI GG'CG_CC Cuts at: 2045 2045 Size: 8821 NcoI C'CATG_G Cuts at: 513 1065 2022 3037 513 Size: 552 957 1015 6297 Fragments arranged by size: 6297 1015 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8821 NgoMIV G'CCGG_C Cuts at: 2047 2047 Size: 8821 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8821 NotI GC'GGCC_GC Cuts at: 6268 6268 Size: 8821 NsiI A_TGCA'T Cuts at: 2831 2831 Size: 8821 NspI r_CATG'y Cuts at: 1132 1229 3322 3360 3481 6179 7724 1132 Size: 97 2093 38 121 2698 1545 2229 Fragments arranged by size: 2698 2229 2093 1545 121 97 38 PasI CC'CwG_GG Cuts at: 1348 2647 2906 3392 4198 1348 Size: 1299 259 486 806 5971 Fragments arranged by size: 5971 1299 806 486 259 PciI A'CATG_T Cuts at: 7720 7720 Size: 8821 PflMI CCAn_nnn'nTGG Cuts at: 1108 2620 5489 5619 5685 1108 Size: 1512 2869 130 66 4244 Fragments arranged by size: 4244 2869 1512 130 66 PfoI T'CCnGG_A Cuts at: 3563 5790 3563 Size: 2227 6594 PmeI GTTT'AAAC Cuts at: 6125 6125 Size: 8821 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5340 5372 6890 6922 8403 8435 5340 Size: 32 1518 32 1481 32 5726 Fragments arranged by size: 5726 1518 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3129 5173 5533 3129 Size: 2044 360 6417 Fragments arranged by size: 6417 2044 360 PshAI GACnn'nnGTC Cuts at: 1613 5279 1613 Size: 3666 5155 PsiI TTA'TAA Cuts at: 6391 6391 Size: 8821 PspOMI G'GGCC_C Cuts at: 5571 5571 Size: 8821 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 5294 5326 5294 Size: 32 8789 PstI C_TGCA'G Cuts at: 830 2581 2694 3521 3708 4408 4677 4704 5193 Size: 1751 113 827 187 700 269 27 489 Cuts at: 5193 5861 6173 830 Size: 668 312 3478 Fragments arranged by size: 3478 1751 827 700 668 489 312 269 187 113 27 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8821 PvuII CAG'CTG Cuts at: 2350 2663 4740 5587 5731 6046 2350 Size: 313 2077 847 144 315 5125 Fragments arranged by size: 5125 2077 847 315 313 144 RsrII CG'GwC_CG Cuts at: 4929 5341 4929 Size: 412 8409 SacI G_AGCT'C Cuts at: 2328 3632 5906 2328 Size: 1304 2274 5243 Fragments arranged by size: 5243 2274 1304 SacII CC_GC'GG Cuts at: 2987 2987 Size: 8821 SalI G'TCGA_C Cuts at: 1936 6163 1936 Size: 4227 4594 SapI GCTCTTCn'nnn_ Cuts at: 4595 4613 5074 4595 Size: 18 461 8342 Fragments arranged by size: 8342 461 18 SbfI CC_TGCA'GG Cuts at: 5193 6173 5193 Size: 980 7841 ScaI AGT'ACT Cuts at: 4020 4760 7069 8781 4020 Size: 740 2309 1712 4060 Fragments arranged by size: 4060 2309 1712 740 SfcI C'TryA_G Cuts at: 826 1045 2111 2577 2690 3517 3704 4404 4516 Size: 219 1066 466 113 827 187 700 112 Cuts at: 4516 4673 4700 5189 5560 5857 6169 7304 7985 Size: 157 27 489 371 297 312 1135 681 Cuts at: 7985 8176 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1066 827 700 681 489 466 371 312 297 219 191 187 157 113 112 27 SfoI GGC'GCC Cuts at: 2046 2046 Size: 8821 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8821 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8821 SmaI CCC'GGG Cuts at: 1872 3134 3593 6148 1872 Size: 1262 459 2555 4545 Fragments arranged by size: 4545 2555 1262 459 SmlI C'TyrA_G Cuts at: 820 1017 1943 2033 2432 2447 3882 4583 5423 Size: 197 926 90 399 15 1435 701 840 Cuts at: 5423 6925 7826 8088 820 Size: 1502 901 262 1553 Fragments arranged by size: 1553 1502 1435 926 901 840 701 399 262 197 90 15 SnaBI TAC'GTA Cuts at: 493 6118 6612 493 Size: 5625 494 2702 Fragments arranged by size: 5625 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8821 SphI G_CATG'C Cuts at: 1132 3322 3360 6179 1132 Size: 2190 38 2819 3774 Fragments arranged by size: 3774 2819 2190 38 SspI AAT'ATT Cuts at: 5 52 6745 5 Size: 47 6693 2081 Fragments arranged by size: 6693 2081 47 StuI AGG'CCT Cuts at: 1517 3077 4565 1517 Size: 1560 1488 5773 Fragments arranged by size: 5773 1560 1488 StyI C'CwwG_G Cuts at: 513 1065 2022 2727 3037 3211 4107 4128 4422 Size: 552 957 705 310 174 896 21 294 Cuts at: 4422 5508 5739 6096 6260 513 Size: 1086 231 357 164 3074 Fragments arranged by size: 3074 1086 957 896 705 552 357 310 294 231 174 164 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2204 2413 3535 4006 5358 6848 6865 Size: 1255 624 209 1122 471 1352 1490 17 Cuts at: 6865 7018 7203 7648 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1490 1352 1255 1122 624 471 445 209 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3846 4018 4758 Size: 274 80 33 51 1443 1869 172 740 Cuts at: 4758 5295 5310 7067 8779 96 Size: 537 15 1757 1712 138 Fragments arranged by size: 1869 1757 1712 1443 740 537 274 172 138 80 51 33 15 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4039 4071 5143 5175 8781 8813 4039 Size: 32 1072 32 3606 32 4047 Fragments arranged by size: 4047 3606 1072 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8821 XbaI T'CTAG_A Cuts at: 6157 6157 Size: 8821 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3035 3044 3733 5453 5974 3035 Size: 9 689 1720 521 5882 Fragments arranged by size: 5882 1720 689 521 9 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8821 XmaI C'CCGG_G Cuts at: 1870 3132 3591 6146 1870 Size: 1262 459 2555 4545 Fragments arranged by size: 4545 2555 1262 459 XmnI GAAnn'nnTTC Cuts at: 2529 4160 6950 2529 Size: 1631 2790 4400 Fragments arranged by size: 4400 2790 1631 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6629 276 Size: 53 83 186 936 5095 2468 Fragments arranged by size: 5095 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BsiWI BstZ17I Bsu36I DraIII EcoRV FseI NruI PacI PmlI PspXI SanDI SexAI SfiI SrfI SwaI