Restriction map for
pFN21ASDA1197
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# NO CUT ENZYMES ( 89 enzymes) #
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AatII AccI AccII AcyI AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AspEI AspHI AspI
AviII AvrII BbeI BfrI BlnI
BsaHI BsiEI BsiHKAI BsiWI Bsp1407I
BspDI BspHI BsrBI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoT22I FseI FspI
Hin1I HpaI KasI KspI MluI
MvnI NarI NdeI NotI NruI
NsiI NspV PacI PflFI PinAI
PmeI PshAI PvuI RcaI RsrII
SacI SacII SalI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tth111I XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 1956 GGTACC G^GTACC
AclI 2214 AACGTT AA^CGTT
Asp718I 1956 GGTACC G^GTACC
BamHI 2191 GGATCC G^GATCC
BanII 3778 GAGCCC G[GA]GC[CT]^C
BbvCI 3888 CCTCAGC CCTCAGC(-5/-2)
BcgI 3203 CGATCCTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1766 TGATCA T^GATCA
BcnI 1516 CCGGG CC^[GC]GG
BglI 2242 GCCAACTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1568 AGATCT A^GATCT
BsmBI 964 GAGACG CGTCTC(1/5)
BsmI 579 GAATGC GAATGC(1/-1)
BspLU11I 301 ACATGT A^CATGT
BsrFI 1471 GCCGGC [GA]^CCGG[CT]
BssSI 3052 CTCGTG CACGAG(-5/-1)
BstAPI 3310 GCAGCTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 3041 GCGC GCG^C
Cfr10I 1471 GCCGGC [GA]^CCGG[CT]
EcoRI 93 GAATTC G^AATTC
FbaI 1766 TGATCA T^GATCA
HaeII 3040 AGCGCC [GA]GCGC^[CT]
HgaI 4057 GCGTC GACGC(5/10)
HhaI 3041 GCGC GCG^C
HinP1I 3041 GCGC G^CGC
HincII 687 GTCAAC GT[CT]^[GA]AC
HindII 687 GTCAAC GT[CT]^[GA]AC
HindIII 1091 AAGCTT A^AGCTT
KpnI 1956 GGTACC GGTAC^C
NaeI 1471 GCCGGC GCC^GGC
NciI 1516 CCGGG CC^[GC]GG
NgoMIV 1471 GCCGGC G^CCGGC
NheI 1159 GCTAGC G^CTAGC
NspI 301 ACATGT [GA]CATG^[CT]
PaeR7I 101 CTCGAG C^TCGAG
PflMI 2016 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 2214 AACGTT AA^CGTT
SapI 2039 GCTCTTC GCTCTTC(1/4)
SexAI 1782 ACCTGGT A^CC[AT]GGT
Van91I 2016 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 544 TCTAGA T^CTAGA
XcmI 2630 CCACTGGGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 101 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccIII 660 TCCGGA T^CCGGA
3094 TCCGGA
AflIII 301 ACATGT A^C[GA][CT]GT
4049 ACGTGT
AocI 1711 CCTAAGG CC^T[ACGT]AGG
3726 CCTGAGG
Asp700I 1146 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1598 GAACAGGTTC
BbrPI 1453 CACGTG CAC^GTG
1798 CACGTG
BlpI 2131 GCTCAGC GC^T[ACGT]AGC
2381 GCTCAGC
Bpu1102I 2131 GCTCAGC GC^T[ACGT]AGC
2381 GCTCAGC
BsaAI 1453 CACGTG [CT]AC^GT[GA]
1798 CACGTG
BsaI 513 GAGACC GGTCTC(1/5)
1587 GGTCTC
BseAI 660 TCCGGA T^CCGGA
3094 TCCGGA
BspEI 660 TCCGGA T^CCGGA
3094 TCCGGA
BstXI 2231 CCACTAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2607 CCAGACTTCTGG
Bsu36I 1711 CCTAAGG CC^T[ACGT]AGG
3726 CCTGAGG
CelII 2131 GCTCAGC GC^T[ACGT]AGC
2381 GCTCAGC
DraII 718 GGGTCCC [GA]G^G[ACGT]CC[CT]
3833 AGGACCT
Eco81I 1711 CCTAAGG CC^T[ACGT]AGG
3726 CCTGAGG
EcoO109I 718 GGGTCCC [GA]G^G[ACGT]CC[CT]
3833 AGGACCT
FauI 1804 CCCGC CCCGC(4/6)
1836 CCCGC
MroI 660 TCCGGA T^CCGGA
3094 TCCGGA
NcoI 1263 CCATGG C^CATGG
2742 CCATGG
PmaCI 1453 CACGTG CAC^GTG
1798 CACGTG
PmlI 1453 CACGTG CAC^GTG
1798 CACGTG
PpuMI 718 GGGTCCC [GA]G^G[AT]CC[CT]
3833 AGGACCT
ScaI 2506 AGTACT AGT^ACT
3542 AGTACT
SspI 4186 AATATT AAT^ATT
4248 AATATT
XmnI 1146 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1598 GAACAGGTTC
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# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI AvaI
AvaII BalI BanI BbsI BbvI
BciVI BfaI BmyI BpmI BpuAI
BsaBI BsaJI BsaWI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DsaI EaeI EarI
EcoNI EcoRII EcoRV EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MfeI
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MunI MvaI
MwoI NdeII NlaIII NlaIV PleI
PshBI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII