Restriction map for
pFN21ASDA1195
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# NO CUT ENZYMES ( 112 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AgeI AocI Aor51HI
ApaI AscI AseI AsnI Asp700I
AviII AvrII BamHI BbeI BbsI
BbvCI BclI BcnI BfrI BglI
BlnI BpuAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmI BspEI
BsrBI BsrFI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cfr10I CpoI DraI DraIII
DrdI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoT22I FbaI
FseI FspI Hin1I HincII HindII
HindIII HpaI KasI KspI MamI
MfeI MluI MroI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
StuI SwaI XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 540 GGTACC G^GTACC
AhdI 1492 GACATTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 934 GTGCAC G^TGCAC
AlwNI 680 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 934 GTGCAC G^TGCAC
Asp718I 540 GGTACC G^GTACC
AspEI 1492 GACATTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 2072 CCCGAG C^[CT]CG[GA]G
BbrPI 1366 CACGTG CAC^GTG
BcgI 825 GCAGGACGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1134 GGATAC GTATCC(6/5)
BlpI 1656 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1656 GCTGAGC GC^T[ACGT]AGC
BsaAI 1366 CACGTG [CT]AC^GT[GA]
BsaI 352 GAGACC GGTCTC(1/5)
BsgI 1468 CTGCAC GTGCAG(16/14)
BsmBI 2121 CGTCTC CGTCTC(1/5)
BsoBI 2072 CCCGAG C^[CT]CG[GA]G
BspDI 1526 ATCGAT AT^CGAT
BssSI 2108 CACGAG CACGAG(-5/-1)
BstAPI 679 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1656 GCTGAGC GC^T[ACGT]AGC
CfoI 785 GCGC GCG^C
ClaI 1526 ATCGAT AT^CGAT
Eam1105I 1492 GACATTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1710 GAATTC G^AATTC
EcoRV 529 GATATC GAT^ATC
HaeII 784 GGCGCT [GA]GCGC^[CT]
HapII 1639 CCGG C^CGG
HgaI 1769 GCGTC GACGC(5/10)
HhaI 785 GCGC GCG^C
HinP1I 785 GCGC G^CGC
HpaII 1639 CCGG C^CGG
KpnI 540 GGTACC GGTAC^C
MspI 1639 CCGG C^CGG
NheI 606 GCTAGC G^CTAGC
PmaCI 1366 CACGTG CAC^GTG
PmlI 1366 CACGTG CAC^GTG
PvuII 899 CAGCTG CAG^CTG
SexAI 632 ACCTGGT A^CC[AT]GGT
SmlI 1618 CTCAAG C^T[CT][GA]AG
SspI 658 AATATT AAT^ATT
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# TWO CUT ENZYMES ( 25 enzymes) #
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AflIII 140 ACATGT A^C[GA][CT]GT
923 ACATGT
AspI 631 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1765 GACAGCGTC
BglII 1179 AGATCT A^GATCT
1913 AGATCT
Bsp1407I 979 TGTACA T^GTACA
1552 TGTACA
BspHI 333 TCATGA T^CATGA
344 TCATGA
BspLU11I 140 ACATGT A^CATGT
923 ACATGT
BsrDI 1749 CATTGC GCAATG(2/0)
1857 GCAATG
BsrGI 979 TGTACA T^GTACA
1552 TGTACA
DraII 404 AGGTCCT [GA]G^G[ACGT]CC[CT]
1163 AGGACCT
EarI 409 CTCTTC CTCTTC(1/4)
1933 CTCTTC
EcoO109I 404 AGGTCCT [GA]G^G[ACGT]CC[CT]
1163 AGGACCT
HphI 484 GGTGA GGTGA(8/7)
1970 TCACC
Ksp632I 409 CTCTTC CTCTTC(1/4)
1933 CTCTTC
MslI 72 CATTCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1971 CACCTCCATG
PflFI 631 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1765 GACAGCGTC
PpuMI 404 AGGTCCT [GA]G^G[AT]CC[CT]
1163 AGGACCT
PstI 253 CTGCAG CTGCA^G
391 CTGCAG
RcaI 333 TCATGA T^CATGA
344 TCATGA
SbfI 252 CCTGCAGG CCTGCA^GG
390 CCTGCAGG
Sse8387I 252 CCTGCAGG CCTGCA^GG
390 CCTGCAGG
SspBI 979 TGTACA T^GTACA
1552 TGTACA
TaqI 1083 TCGA T^CGA
1527 TCGA
Tsp45I 1390 GTGAC ^GT[GC]AC
1969 GTCAC
Tth111I 631 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1765 GACAGCGTC
TthHB8I 1083 TCGA T^CGA
1527 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BalI BanI
BanII BbvI BfaI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DsaI EaeI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HinfI ItaI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MspA1I MvaI
MwoI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TfiI Tru9I TseI Tsp509I
TspRI Van91I XcmI XhoII