Restriction map for
pFN21ASDA1195
(Linear) (Six-base) MAPSORT of: KIAA1195.seq Check: 1840 from: 1 to: 2127
KIAA1195.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 125 246 260 2127
Size: 125 121 14 1867
Fragments arranged by size:
1867 125 121 14
Acc65I G'GTAC_C
Cuts at: 0 540 2127
Size: 540 1587
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 396 1124 1431 2011 2127
Size: 396 728 307 580 116
Fragments arranged by size:
728 580 396 307 116
AflIII A'CryG_T
Cuts at: 0 140 923 2127
Size: 140 783 1204
Fragments arranged by size:
1204 783 140
AhdI GACnn_n'nnGTC
Cuts at: 0 1497 2127
Size: 1497 630
AlwNI CAG_nnn'CTG
Cuts at: 0 685 2127
Size: 685 1442
ApaLI G'TGCA_C
Cuts at: 0 934 2127
Size: 934 1193
ApoI r'AATT_y
Cuts at: 0 696 1441 1710 2127
Size: 696 745 269 417
Fragments arranged by size:
745 696 417 269
AvaI C'yCGr_G
Cuts at: 0 2072 2127
Size: 2072 55
BanI G'GyrC_C
Cuts at: 0 29 127 540 1888 2127
Size: 29 98 413 1348 239
Fragments arranged by size:
1348 413 239 98 29
BanII G_rGCy'C
Cuts at: 0 1083 1376 1619 2127
Size: 1083 293 243 508
Fragments arranged by size:
1083 508 293 243
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 814 848 2127
Size: 814 34 1279
Fragments arranged by size:
1279 814 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1128 2127
Size: 1128 999
BglII A'GATC_T
Cuts at: 0 1179 1913 2127
Size: 1179 734 214
Fragments arranged by size:
1179 734 214
BlpI GC'TnA_GC
Cuts at: 0 1657 2127
Size: 1657 470
Bme1580I G_kGCm'C
Cuts at: 0 32 265 938 2127
Size: 32 233 673 1189
Fragments arranged by size:
1189 673 233 32
BmgBI CAC'GTC
Cuts at: 0 1233 2127
Size: 1233 894
BmrI ACTGGGnnnn_n'
Cuts at: 0 353 2127
Size: 353 1774
BmtI G_CTAG'C
Cuts at: 0 610 2127
Size: 610 1517
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1036 1183 1634 1753 1917 2127
Size: 1036 147 451 119 164 210
Fragments arranged by size:
1036 451 210 164 147 119
Bpu10I CC'TnA_GC
Cuts at: 0 1022 2127
Size: 1022 1105
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1603 2127
Size: 1603 524
BsaI GGTCTCn'nnnn_
Cuts at: 0 346 2127
Size: 346 1781
BsaAI yAC'GTr
Cuts at: 0 1368 2127
Size: 1368 759
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 500 530 2127
Size: 500 30 1597
Fragments arranged by size:
1597 500 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 157 379 478 524 2127
Size: 157 222 99 46 1603
Fragments arranged by size:
1603 222 157 99 46
BseYI C'CCAG_C
Cuts at: 0 1753 1781 1952 2127
Size: 1753 28 171 175
Fragments arranged by size:
1753 175 171 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1453 2127
Size: 1453 674
BsiHKAI G_wGCw'C
Cuts at: 0 161 677 938 1083 1376 1619 1988 2021
Size: 161 516 261 145 293 243 369 33
Cuts at: 2021 2127
Size: 106
Fragments arranged by size:
516 369 293 261 243 161 145 106
33
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2127
Size: 2127
Bsp1286I G_dGCh'C
Cuts at: 0 32 161 265 677 938 1083 1376 1619
Size: 32 129 104 412 261 145 293 243
Cuts at: 1619 1988 2021 2127
Size: 369 33 106
Fragments arranged by size:
412 369 293 261 243 145 129 106
104 33 32
BspHI T'CATG_A
Cuts at: 0 333 344 2127
Size: 333 11 1783
Fragments arranged by size:
1783 333 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 125 174 246 260 1175 2127
Size: 125 49 72 14 915 952
Fragments arranged by size:
952 915 125 72 49 14
BsrDI GCAATG_nn'
Cuts at: 0 1748 1864 2127
Size: 1748 116 263
Fragments arranged by size:
1748 263 116
BsrGI T'GTAC_A
Cuts at: 0 979 1552 2127
Size: 979 573 575
Fragments arranged by size:
979 575 573
BssSI C'ACGA_G
Cuts at: 0 2108 2127
Size: 2108 19
BstAPI GCAn_nnn'nTGC
Cuts at: 0 685 2127
Size: 685 1442
BstYI r'GATC_y
Cuts at: 0 16 415 1179 1913 2127
Size: 16 399 764 734 214
Fragments arranged by size:
764 734 399 214 16
BtgI C'CryG_G
Cuts at: 0 287 842 1881 2127
Size: 287 555 1039 246
Fragments arranged by size:
1039 555 287 246
BtsI GCAGTG_nn'
Cuts at: 0 63 521 1136 2127
Size: 63 458 615 991
Fragments arranged by size:
991 615 458 63
ClaI AT'CG_AT
Cuts at: 0 1527 2127
Size: 1527 600
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 22 57 840 875 2127
Size: 22 35 783 35 1252
Fragments arranged by size:
1252 783 35 35 22
EaeI y'GGCC_r
Cuts at: 0 242 304 645 759 2127
Size: 242 62 341 114 1368
Fragments arranged by size:
1368 341 242 114 62
EarI CTCTTCn'nnn_
Cuts at: 0 415 1939 2127
Size: 415 1524 188
Fragments arranged by size:
1524 415 188
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 960 2127
Size: 960 1167
EcoICRI GAG'CTC
Cuts at: 0 1081 1374 1617 2127
Size: 1081 293 243 510
Fragments arranged by size:
1081 510 293 243
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 396 1036 1124 1183 1431 1634 1753 1917
Size: 396 640 88 59 248 203 119 164
Cuts at: 1917 2011 2127
Size: 94 116
Fragments arranged by size:
640 396 248 203 164 119 116 94
88 59
EcoO109I rG'GnC_Cy
Cuts at: 0 405 1164 2127
Size: 405 759 963
Fragments arranged by size:
963 759 405
EcoRI G'AATT_C
Cuts at: 0 1710 2127
Size: 1710 417
EcoRV GAT'ATC
Cuts at: 0 531 2127
Size: 531 1596
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 426 2127
Size: 426 1701
HaeII r_GCGC'y
Cuts at: 0 788 2127
Size: 788 1339
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 515 547 1483 1515 1520 1552 1832 1864
Size: 515 32 936 32 5 32 280 32
Cuts at: 1864 2127
Size: 263
Fragments arranged by size:
936 515 280 263 32 32 32 32
5
KpnI G_GTAC'C
Cuts at: 0 544 2127
Size: 544 1583
MscI TGG'CCA
Cuts at: 0 244 306 647 761 2127
Size: 244 62 341 114 1366
Fragments arranged by size:
1366 341 244 114 62
MslI CAynn'nnrTG
Cuts at: 0 76 1975 2127
Size: 76 1899 152
Fragments arranged by size:
1899 152 76
MspA1I CmG'CkG
Cuts at: 0 302 901 1345 2127
Size: 302 599 444 782
Fragments arranged by size:
782 599 444 302
NcoI C'CATG_G
Cuts at: 0 287 842 1881 2127
Size: 287 555 1039 246
Fragments arranged by size:
1039 555 287 246
NheI G'CTAG_C
Cuts at: 0 606 2127
Size: 606 1521
NspI r_CATG'y
Cuts at: 0 144 927 2052 2127
Size: 144 783 1125 75
Fragments arranged by size:
1125 783 144 75
PasI CC'CwG_GG
Cuts at: 0 1354 1582 2127
Size: 1354 228 545
Fragments arranged by size:
1354 545 228
PciI A'CATG_T
Cuts at: 0 140 923 2127
Size: 140 783 1204
Fragments arranged by size:
1204 783 140
PflMI CCAn_nnn'nTGG
Cuts at: 0 240 293 768 1686 2127
Size: 240 53 475 918 441
Fragments arranged by size:
918 475 441 240 53
PfoI T'CCnGG_A
Cuts at: 0 1894 2127
Size: 1894 233
PmlI CAC'GTG
Cuts at: 0 1368 2127
Size: 1368 759
PpuMI rG'GwC_Cy
Cuts at: 0 405 1164 2127
Size: 405 759 963
Fragments arranged by size:
963 759 405
PstI C_TGCA'G
Cuts at: 0 257 395 2127
Size: 257 138 1732
Fragments arranged by size:
1732 257 138
PvuII CAG'CTG
Cuts at: 0 901 2127
Size: 901 1226
SacI G_AGCT'C
Cuts at: 0 1083 1376 1619 2127
Size: 1083 293 243 508
Fragments arranged by size:
1083 508 293 243
SbfI CC_TGCA'GG
Cuts at: 0 257 395 2127
Size: 257 138 1732
Fragments arranged by size:
1732 257 138
SexAI A'CCwGG_T
Cuts at: 0 632 2127
Size: 632 1495
SfcI C'TryA_G
Cuts at: 0 253 391 1605 2127
Size: 253 138 1214 522
Fragments arranged by size:
1214 522 253 138
SmlI C'TyrA_G
Cuts at: 0 1618 2127
Size: 1618 509
SspI AAT'ATT
Cuts at: 0 660 2127
Size: 660 1467
StyI C'CwwG_G
Cuts at: 0 287 842 865 986 1881 2127
Size: 287 555 23 121 895 246
Fragments arranged by size:
895 555 287 246 121 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 508 999 2127
Size: 508 491 1128
Fragments arranged by size:
1128 508 491
TatI w'GTAC_w
Cuts at: 0 979 1552 1745 2127
Size: 979 573 193 382
Fragments arranged by size:
979 573 382 193
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 500 532 1352 1384 1803 1835 1978 2010
Size: 500 32 820 32 419 32 143 32
Cuts at: 2010 2127
Size: 117
Fragments arranged by size:
820 500 419 143 117 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 634 1768 2127
Size: 634 1134 359
Fragments arranged by size:
1134 634 359
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 121 624 1728 2127
Size: 121 503 1104 399
Fragments arranged by size:
1104 503 399 121
Enzymes that do cut:
AarI Acc65I AcuI AflIII AhdI AlwNI ApaLI ApoI
AvaI BanI BanII BcgI BciVI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI
BspMI BsrDI BsrGI BssSI BstAPI BstYI BtgI BtsI
ClaI CspCI EaeI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI HaeII Hin4I KpnI MscI MslI
MspA1I NcoI NheI NspI PasI PciI PflMI PfoI
PmlI PpuMI PstI PvuII SacI SbfI SexAI SfcI
SmlI SspI StyI TaqII TatI TstI Tth111I XcmI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AleI AlfI
AloI ApaI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbsI BbvCI BclI BfrBI BglI BplI BsaBI
BsaHI BsaWI BsiEI BsiWI BsmI BspEI BsrBI BsrFI
BssHII BstBI BstEII BstXI BstZ17I Bsu36I BtgZI DraI
DraIII DrdI EagI EcoNI FseI FspI FspAI HincII
HindIII HpaI KasI MfeI MluI MmeI NaeI NarI
NdeI NgoMIV NotI NruI NsiI PacI PmeI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI ScaI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StuI SwaI XbaI
XhoI XmaI XmnI ZraI