(Circular) (Six-base) MAPSORT of: pFN21AA1193.seq Check: 5109 from: 1 to: 6340 pFN21AA1193 6340 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4150 278 Size: 53 83 186 936 2614 2468 Fragments arranged by size: 2614 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3683 1937 Size: 1746 4594 Acc65I G'GTAC_C Cuts at: 3661 3661 Size: 6340 AclI AA'CG_TT Cuts at: 1563 4467 4840 1563 Size: 2904 373 3063 Fragments arranged by size: 3063 2904 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4403 5787 6055 1632 Size: 2771 1384 268 1917 Fragments arranged by size: 2771 1917 1384 268 AfeI AGC'GCT Cuts at: 3050 3050 Size: 6340 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6143 AflIII A'CryG_T Cuts at: 1203 5239 6216 1203 Size: 4036 977 1327 Fragments arranged by size: 4036 1327 977 AgeI A'CCGG_T Cuts at: 5171 5171 Size: 6340 AhdI GACnn_n'nnGTC Cuts at: 5069 5069 Size: 6340 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6340 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2273 2445 1184 Size: 1089 172 5079 Fragments arranged by size: 5079 1089 172 AlwNI CAG_nnn'CTG Cuts at: 2815 3130 3304 4083 5655 2815 Size: 315 174 779 1572 3500 Fragments arranged by size: 3500 1572 779 315 174 ApaLI G'TGCA_C Cuts at: 4397 5553 4397 Size: 1156 5184 ApoI r'AATT_y Cuts at: 1493 3649 3875 4027 6136 1493 Size: 2156 226 152 2109 1697 Fragments arranged by size: 2156 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4894 160 Size: 872 3862 1606 Fragments arranged by size: 3862 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6340 AvaI C'yCGr_G Cuts at: 1870 1943 2057 2194 3593 3665 1870 Size: 73 114 137 1399 72 4545 Fragments arranged by size: 4545 1399 137 114 73 72 BamHI G'GATC_C Cuts at: 3478 3670 4037 3478 Size: 192 367 5781 Fragments arranged by size: 5781 367 192 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2199 3661 5116 618 Size: 325 217 627 412 1462 1455 1842 Fragments arranged by size: 1842 1462 1455 627 412 325 217 BanII G_rGCy'C Cuts at: 1391 2120 2901 3246 3612 3659 1391 Size: 729 781 345 366 47 4072 Fragments arranged by size: 4072 781 729 366 345 47 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2523 2679 928 Size: 971 624 156 4589 Fragments arranged by size: 4589 971 624 156 BbvCI CC'TCA_GC Cuts at: 2624 2772 3470 2624 Size: 148 698 5494 Fragments arranged by size: 5494 698 148 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2021 2055 2374 2408 4531 4565 1903 Size: 34 84 34 319 34 2123 34 3678 Fragments arranged by size: 3678 2123 319 84 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4232 5442 4232 Size: 1210 5130 BclI T'GATC_A Cuts at: 1548 1548 Size: 6340 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4951 136 Size: 107 122 71 631 605 3279 1525 Fragments arranged by size: 3279 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2658 6335 2658 Size: 3677 2663 BlpI GC'TnA_GC Cuts at: 3757 3757 Size: 6340 Bme1580I G_kGCm'C Cuts at: 1790 2075 2204 3587 4401 5557 1790 Size: 285 129 1383 814 1156 2573 Fragments arranged by size: 2573 1383 1156 814 285 129 BmgBI CAC'GTC Cuts at: 1318 2338 3553 1318 Size: 1020 1215 4105 Fragments arranged by size: 4105 1215 1020 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3198 4071 5029 Size: 416 53 83 399 24 1781 873 958 Cuts at: 5029 442 Size: 1753 Fragments arranged by size: 1781 1753 958 873 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6340 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2035 2209 2514 2548 2578 2915 2035 Size: 174 305 34 30 337 5460 Fragments arranged by size: 5460 337 305 174 34 30 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2142 2246 3439 5000 1239 Size: 903 104 1193 1561 2579 Fragments arranged by size: 2579 1561 1193 903 104 Bpu10I CC'TnA_GC Cuts at: 2624 2772 3470 2624 Size: 148 698 5494 Fragments arranged by size: 5494 698 148 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4465 5330 5628 4465 Size: 865 298 5177 Fragments arranged by size: 5177 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2053 2118 3239 5003 882 Size: 617 554 65 1121 1764 2219 Fragments arranged by size: 2219 1764 1121 617 554 65 BsaAI yAC'GTr Cuts at: 493 1204 3582 4131 493 Size: 711 2378 549 2702 Fragments arranged by size: 2702 2378 711 549 BsaBI GATnn'nnATC Cuts at: 6334 6334 Size: 6340 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2297 3399 4147 4529 Size: 53 83 186 936 764 1102 748 382 Cuts at: 4529 5164 275 Size: 635 1451 Fragments arranged by size: 1451 1102 936 764 748 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2487 3104 3388 3481 4773 5171 5445 5592 6003 Size: 617 284 93 1292 398 274 147 411 Cuts at: 6003 2487 Size: 2824 Fragments arranged by size: 2824 1292 617 411 398 284 274 147 93 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2020 2050 2225 2255 2453 Size: 30 165 30 617 30 175 30 198 Cuts at: 2453 2483 2714 2744 2778 2808 3599 3629 1178 Size: 30 231 30 34 30 791 30 3889 Fragments arranged by size: 3889 791 617 231 198 175 165 34 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2008 2201 2234 2533 2570 2735 2864 2933 Size: 822 193 33 299 37 165 129 69 Cuts at: 2933 3500 1186 Size: 567 4026 Fragments arranged by size: 4026 822 567 299 193 165 129 69 37 33 BseYI C'CCAG_C Cuts at: 2023 2693 3066 3079 3343 3358 3499 5543 6039 Size: 670 373 13 264 15 141 2044 496 Cuts at: 6039 2023 Size: 2324 Fragments arranged by size: 2324 2044 670 496 373 264 141 15 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2618 2944 1163 Size: 573 882 326 4559 Fragments arranged by size: 4559 882 573 326 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3160 3228 3790 4551 5579 1540 Size: 275 186 1159 68 562 761 1028 2301 Fragments arranged by size: 2301 1159 1028 761 562 275 186 68 BsiHKAI G_wGCw'C Cuts at: 2064 2940 4401 4486 5557 2064 Size: 876 1461 85 1071 2847 Fragments arranged by size: 2847 1461 1071 876 85 BsmI GAATG_Cn' Cuts at: 3851 3944 3851 Size: 93 6247 BsmBI CGTCTCn'nnnn_ Cuts at: 2411 2411 Size: 6340 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2064 2075 2120 2204 2901 2940 3246 Size: 399 274 11 45 84 697 39 306 Cuts at: 3246 3587 3612 3659 4401 4486 5557 1391 Size: 341 25 47 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 697 399 341 306 274 85 84 47 45 39 25 11 BspEI T'CCGG_A Cuts at: 3104 3388 3481 6003 3104 Size: 284 93 2522 3441 Fragments arranged by size: 3441 2522 284 93 BspHI T'CATG_A Cuts at: 4229 5959 4229 Size: 1730 4610 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2640 3644 3695 844 Size: 1796 1004 51 3489 Fragments arranged by size: 3489 1796 1004 51 BsrBI CCG'CTC Cuts at: 1394 1823 4227 1394 Size: 429 2404 3507 Fragments arranged by size: 3507 2404 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4835 5009 65 Size: 1183 3587 174 1396 Fragments arranged by size: 3587 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2643 3227 4984 5171 1700 Size: 110 833 584 1757 187 2869 Fragments arranged by size: 2869 1757 833 584 187 110 BsrGI T'GTAC_A Cuts at: 96 2730 96 Size: 2634 3706 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6340 BssSI C'ACGA_G Cuts at: 2462 2756 4400 5412 2462 Size: 294 1644 1012 3390 Fragments arranged by size: 3390 1644 1012 294 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6340 BstBI TT'CG_AA Cuts at: 5144 5144 Size: 6340 BstEII G'GTnAC_C Cuts at: 1184 3520 1184 Size: 2336 4004 BstXI CCAn_nnnn'nTGG Cuts at: 3463 3463 Size: 6340 BstYI r'GATC_y Cuts at: 1972 2148 2495 2658 3202 3478 3670 4037 4422 Size: 176 347 163 544 276 192 367 385 Cuts at: 4422 4439 5891 5977 5989 6335 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 544 385 367 347 346 276 192 176 163 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2112 3111 3417 513 Size: 552 939 108 999 306 3436 Fragments arranged by size: 3436 999 939 552 306 108 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5601 Fragments arranged by size: 5601 683 56 BtsI GCAGTG_nn' Cuts at: 2109 3381 3856 4650 4670 2109 Size: 1272 475 794 20 3779 Fragments arranged by size: 3779 1272 794 475 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6340 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2085 2120 529 Size: 35 1521 35 4749 Fragments arranged by size: 4749 1521 35 35 DraI TTT'AAA Cuts at: 3644 3644 Size: 6340 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2696 5347 809 Size: 507 1380 2651 1802 Fragments arranged by size: 2651 1802 1380 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2763 3157 3225 Size: 54 1425 50 275 18 933 394 68 Cuts at: 3225 3351 3414 3567 3787 4676 6305 8 Size: 126 63 153 220 889 1629 43 Fragments arranged by size: 1629 1425 933 889 394 275 220 153 126 68 63 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3157 3225 3787 1537 Size: 275 1345 68 562 4090 Fragments arranged by size: 4090 1345 562 275 68 EarI CTCTTCn'nnn_ Cuts at: 1353 2403 2409 2483 2539 2571 2910 4270 1353 Size: 1050 6 74 56 32 339 1360 3423 Fragments arranged by size: 3423 1360 1050 339 74 56 32 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 4927 5301 5447 387 Size: 1064 138 435 2903 374 146 1280 Fragments arranged by size: 2903 1280 1064 435 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2142 2246 3439 4403 5000 5787 6055 Size: 393 510 104 1193 964 597 787 268 Cuts at: 6055 1239 Size: 1524 Fragments arranged by size: 1524 1193 964 787 597 510 393 268 104 EcoNI CCTnn'n_nnAGG Cuts at: 2975 3434 2975 Size: 459 5881 EcoO109I rG'GnC_Cy Cuts at: 2603 2689 2854 3127 2603 Size: 86 165 273 5816 Fragments arranged by size: 5816 273 165 86 EcoRI G'AATT_C Cuts at: 3649 4027 3649 Size: 378 5962 FspI TGC'GCA Cuts at: 2993 3197 4846 2993 Size: 204 1649 4487 Fragments arranged by size: 4487 1649 204 FspAI rTGC'GCAy Cuts at: 2993 3197 2993 Size: 204 6136 HaeII r_GCGC'y Cuts at: 1678 2816 3052 5487 1678 Size: 1138 236 2435 2531 Fragments arranged by size: 2531 2435 1138 236 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2020 2052 2138 2170 2225 Size: 32 135 32 448 32 86 32 55 Cuts at: 2225 2257 2408 2440 3347 3379 3599 3631 4981 Size: 32 151 32 907 32 220 32 1350 Cuts at: 4981 5013 5055 5087 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1350 907 448 220 151 135 86 55 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3684 3930 669 Size: 988 281 1746 246 3079 Fragments arranged by size: 3079 1746 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6340 HpaI GTT'AAC Cuts at: 3930 3930 Size: 6340 KpnI G_GTAC'C Cuts at: 3665 3665 Size: 6340 MfeI C'AATT_G Cuts at: 3939 3939 Size: 6340 MluI A'CGCG_T Cuts at: 6216 6216 Size: 6340 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2496 2571 3024 5454 5638 1305 Size: 1191 75 453 2430 184 2007 Fragments arranged by size: 2430 2007 1191 453 184 75 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4518 Fragments arranged by size: 4518 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4298 4657 4816 518 Size: 648 3132 359 159 2042 Fragments arranged by size: 3132 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2680 3304 3320 3377 3474 3756 4433 5581 Size: 1074 624 16 57 97 282 677 1148 Cuts at: 5581 5826 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 1074 677 624 282 245 97 57 16 NcoI C'CATG_G Cuts at: 513 1065 2004 2112 513 Size: 552 939 108 4741 Fragments arranged by size: 4741 939 552 108 NdeI CA'TA_TG Cuts at: 387 387 Size: 6340 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6340 NotI GC'GGCC_GC Cuts at: 3787 3787 Size: 6340 NspI r_CATG'y Cuts at: 1132 1229 2260 3698 5243 1132 Size: 97 1031 1438 1545 2229 Fragments arranged by size: 2229 1545 1438 1031 97 PasI CC'CwG_GG Cuts at: 1348 2075 1348 Size: 727 5613 PciI A'CATG_T Cuts at: 5239 5239 Size: 6340 PflMI CCAn_nnn'nTGG Cuts at: 1108 3130 3304 1108 Size: 2022 174 4144 Fragments arranged by size: 4144 2022 174 PmeI GTTT'AAAC Cuts at: 3644 3644 Size: 6340 PmlI CAC'GTG Cuts at: 3582 3582 Size: 6340 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4409 4441 5922 5954 4409 Size: 32 1481 32 4795 Fragments arranged by size: 4795 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2603 2689 3127 2603 Size: 86 438 5816 Fragments arranged by size: 5816 438 86 PshAI GACnn'nnGTC Cuts at: 1613 3268 1613 Size: 1655 4685 PsiI TTA'TAA Cuts at: 3910 3910 Size: 6340 PspXI vC'TCGA_Gb Cuts at: 2057 2194 2057 Size: 137 6203 PstI C_TGCA'G Cuts at: 830 3692 830 Size: 2862 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6340 PvuII CAG'CTG Cuts at: 2680 3304 3377 2680 Size: 624 73 5643 Fragments arranged by size: 5643 624 73 SalI G'TCGA_C Cuts at: 1936 3682 1936 Size: 1746 4594 SanDI GG'GwC_CC Cuts at: 2689 2689 Size: 6340 SapI GCTCTTCn'nnn_ Cuts at: 2539 2539 Size: 6340 SbfI CC_TGCA'GG Cuts at: 3692 3692 Size: 6340 ScaI AGT'ACT Cuts at: 3023 4588 6300 3023 Size: 1565 1712 3063 Fragments arranged by size: 3063 1712 1565 SfcI C'TryA_G Cuts at: 826 1045 2164 3688 4823 5504 5695 826 Size: 219 1119 1524 1135 681 191 1471 Fragments arranged by size: 1524 1471 1135 1119 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6340 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6340 SmaI CCC'GGG Cuts at: 1872 3595 3667 1872 Size: 1723 72 4545 Fragments arranged by size: 4545 1723 72 SmlI C'TyrA_G Cuts at: 820 1017 1943 2057 2194 4444 5345 5607 820 Size: 197 926 114 137 2250 901 262 1553 Fragments arranged by size: 2250 1553 926 901 262 197 137 114 SnaBI TAC'GTA Cuts at: 493 4131 493 Size: 3638 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6340 SphI G_CATG'C Cuts at: 1132 3698 1132 Size: 2566 3774 SspI AAT'ATT Cuts at: 5 52 4264 5 Size: 47 4212 2081 Fragments arranged by size: 4212 2081 47 StuI AGG'CCT Cuts at: 1517 2015 2192 2468 1517 Size: 498 177 276 5389 Fragments arranged by size: 5389 498 276 177 StyI C'CwwG_G Cuts at: 513 1065 2004 2112 2214 3407 3435 3779 513 Size: 552 939 108 102 1193 28 344 3074 Fragments arranged by size: 3074 1193 939 552 344 108 102 28 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3508 4367 4384 4537 4722 5167 325 Size: 1255 1928 859 17 153 185 445 1498 Fragments arranged by size: 1928 1498 1255 859 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2730 3021 4586 Size: 274 80 33 51 1443 753 291 1565 Cuts at: 4586 6298 96 Size: 1712 138 Fragments arranged by size: 1712 1565 1443 753 291 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2453 2485 2778 2810 6300 6332 2453 Size: 32 293 32 3490 32 2461 Fragments arranged by size: 3490 2461 293 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6340 XbaI T'CTAG_A Cuts at: 3676 3676 Size: 6340 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2221 3131 3409 2221 Size: 910 278 5152 Fragments arranged by size: 5152 910 278 XhoI C'TCGA_G Cuts at: 1943 2057 2194 1943 Size: 114 137 6089 Fragments arranged by size: 6089 137 114 XmaI C'CCGG_G Cuts at: 1870 3593 3665 1870 Size: 1723 72 4545 Fragments arranged by size: 4545 1723 72 XmnI GAAnn'nnTTC Cuts at: 2234 3560 4469 2234 Size: 1326 909 4105 Fragments arranged by size: 4105 1326 909 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4148 276 Size: 53 83 186 936 2614 2468 Fragments arranged by size: 2614 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PsrI RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI