(Circular) (Six-base) MAPSORT of: pFN21ASDA1187.seq Check: 1664 from: 1 to: 7138 pFN21ASDA1187 7138 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4948 278 Size: 53 83 186 936 3412 2468 Fragments arranged by size: 3412 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4481 1937 Size: 2544 4594 Acc65I G'GTAC_C Cuts at: 4459 4459 Size: 7138 AclI AA'CG_TT Cuts at: 1563 5265 5638 1563 Size: 3702 373 3063 Fragments arranged by size: 3702 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2310 5201 6585 6853 1632 Size: 678 2891 1384 268 1917 Fragments arranged by size: 2891 1917 1384 678 268 AfeI AGC'GCT Cuts at: 2651 2711 2651 Size: 60 7078 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6941 AflIII A'CryG_T Cuts at: 1203 6037 7014 1203 Size: 4834 977 1327 Fragments arranged by size: 4834 1327 977 AgeI A'CCGG_T Cuts at: 5969 5969 Size: 7138 AhdI GACnn_n'nnGTC Cuts at: 5867 5867 Size: 7138 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7138 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2616 3357 4262 1184 Size: 1432 741 905 4060 Fragments arranged by size: 4060 1432 905 741 AlwNI CAG_nnn'CTG Cuts at: 3238 3623 3650 3985 4142 4881 6453 3238 Size: 385 27 335 157 739 1572 3923 Fragments arranged by size: 3923 1572 739 385 335 157 27 ApaI G_GGCC'C Cuts at: 2330 2731 3480 2330 Size: 401 749 5988 Fragments arranged by size: 5988 749 401 ApaLI G'TGCA_C Cuts at: 3047 5195 6351 3047 Size: 2148 1156 3834 Fragments arranged by size: 3834 2148 1156 ApoI r'AATT_y Cuts at: 1493 4447 4673 4825 6934 1493 Size: 2954 226 152 2109 1697 Fragments arranged by size: 2954 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5692 160 Size: 872 4660 1606 Fragments arranged by size: 4660 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7138 AvaI C'yCGr_G Cuts at: 1870 1943 2633 3207 3402 3479 3741 3837 4463 Size: 73 690 574 195 77 262 96 626 Cuts at: 4463 1870 Size: 4545 Fragments arranged by size: 4545 690 626 574 262 195 96 77 73 AvrII C'CTAG_G Cuts at: 2252 2252 Size: 7138 BamHI G'GATC_C Cuts at: 4468 4835 4468 Size: 367 6771 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2559 3407 3547 3559 3686 Size: 325 217 627 772 848 140 12 127 Cuts at: 3686 3732 4459 5914 618 Size: 46 727 1455 1842 Fragments arranged by size: 1842 1455 848 772 727 627 325 217 140 127 46 12 BanII G_rGCy'C Cuts at: 1391 2234 2330 2731 3288 3464 3480 4206 4457 Size: 843 96 401 557 176 16 726 251 Cuts at: 4457 1391 Size: 4072 Fragments arranged by size: 4072 843 726 557 401 251 176 96 16 BbeI G_GCGC'C Cuts at: 2563 3563 3736 2563 Size: 1000 173 5965 Fragments arranged by size: 5965 1000 173 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2690 928 Size: 971 791 5376 Fragments arranged by size: 5376 971 791 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5329 5363 1903 Size: 34 3392 34 3678 Fragments arranged by size: 3678 3392 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5030 6240 5030 Size: 1210 5928 BclI T'GATC_A Cuts at: 1548 1548 Size: 7138 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2492 2939 3545 Size: 107 122 71 631 605 820 447 606 Cuts at: 3545 5749 136 Size: 2204 1525 Fragments arranged by size: 2204 1525 820 631 606 605 447 122 107 71 BglII A'GATC_T Cuts at: 7133 7133 Size: 7138 BlpI GC'TnA_GC Cuts at: 2777 3287 4555 2777 Size: 510 1268 5360 Fragments arranged by size: 5360 1268 510 Bme1580I G_kGCm'C Cuts at: 1790 2330 2731 2996 3051 3202 3259 3480 5199 Size: 540 401 265 55 151 57 221 1719 Cuts at: 5199 6355 1790 Size: 1156 2573 Fragments arranged by size: 2573 1719 1156 540 401 265 221 151 57 55 BmgBI CAC'GTC Cuts at: 1318 2688 1318 Size: 1370 5768 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4869 5827 442 Size: 416 53 83 399 24 3452 958 1753 Fragments arranged by size: 3452 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7138 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2251 2251 Size: 7138 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2091 2525 2531 2588 2630 2730 4007 4067 Size: 852 434 6 57 42 100 1277 60 Cuts at: 4067 5798 1239 Size: 1731 2579 Fragments arranged by size: 2579 1731 1277 852 434 100 60 57 42 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3206 4373 5263 6128 6426 3206 Size: 1167 890 865 298 3918 Fragments arranged by size: 3918 1167 890 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3602 5801 882 Size: 617 2103 2199 2219 Fragments arranged by size: 2219 2199 2103 617 BsaAI yAC'GTr Cuts at: 493 1204 2916 3254 4435 4929 493 Size: 711 1712 338 1181 494 2702 Fragments arranged by size: 2702 1712 1181 711 494 338 BsaBI GATnn'nnATC Cuts at: 7132 7132 Size: 7138 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2560 2816 3023 3560 Size: 53 83 186 936 1027 256 207 537 Cuts at: 3560 3733 4945 5327 5962 275 Size: 173 1212 382 635 1451 Fragments arranged by size: 1451 1212 1027 936 635 537 382 256 207 186 173 83 53 BsaWI w'CCGG_w Cuts at: 3115 5571 5969 6243 6390 6801 3115 Size: 2456 398 274 147 411 3452 Fragments arranged by size: 3452 2456 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3367 3397 4148 4178 4208 Size: 30 165 30 1964 30 751 30 30 Cuts at: 4208 4238 1178 Size: 30 4078 Fragments arranged by size: 4078 1964 751 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2337 2528 2585 3461 3776 3824 3887 3926 Size: 1151 191 57 876 315 48 63 39 Cuts at: 3926 3956 4064 4214 1186 Size: 30 108 150 4110 Fragments arranged by size: 4110 1151 876 315 191 150 108 63 57 48 39 30 BseYI C'CCAG_C Cuts at: 2047 2065 3356 3464 3535 3574 3643 4282 6341 Size: 18 1291 108 71 39 69 639 2059 Cuts at: 6341 6837 2047 Size: 496 2348 Fragments arranged by size: 2348 2059 1291 639 496 108 71 69 39 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2622 2717 3049 3167 4211 4424 1163 Size: 573 886 95 332 118 1044 213 3877 Fragments arranged by size: 3877 1044 886 573 332 213 118 95 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2875 2962 3131 3959 4588 5349 Size: 275 186 874 87 169 828 629 761 Cuts at: 5349 6377 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 874 828 761 629 275 186 169 87 BsiHKAI G_wGCw'C Cuts at: 3051 3288 5199 5284 6355 3051 Size: 237 1911 85 1071 3834 Fragments arranged by size: 3834 1911 1071 237 85 BsmI GAATG_Cn' Cuts at: 2197 3807 4649 4742 2197 Size: 1610 842 93 4593 Fragments arranged by size: 4593 1610 842 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2234 2330 2731 2996 3051 3202 3259 Size: 399 444 96 401 265 55 151 57 Cuts at: 3259 3288 3464 3480 4206 4457 5199 5284 6355 Size: 29 176 16 726 251 742 85 1071 Cuts at: 6355 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 726 444 401 399 265 251 176 151 96 85 57 55 29 16 BspEI T'CCGG_A Cuts at: 6801 6801 Size: 7138 BspHI T'CATG_A Cuts at: 4056 5027 6757 4056 Size: 971 1730 4437 Fragments arranged by size: 4437 1730 971 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2233 4405 4493 844 Size: 1389 2172 88 3489 Fragments arranged by size: 3489 2172 1389 88 BsrBI CCG'CTC Cuts at: 1394 1823 2031 2455 2467 3113 3175 3772 3847 Size: 429 208 424 12 646 62 597 75 Cuts at: 3847 3922 3976 3991 4024 5025 1394 Size: 75 54 15 33 1001 3507 Fragments arranged by size: 3507 1001 646 597 429 424 208 75 75 62 54 33 15 12 BsrDI GCAATG_nn' Cuts at: 65 1248 5633 5807 65 Size: 1183 4385 174 1396 Fragments arranged by size: 4385 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2556 2562 2986 3065 3126 3142 3544 Size: 110 746 6 424 79 61 16 402 Cuts at: 3544 3556 3683 5782 5969 1700 Size: 12 127 2099 187 2869 Fragments arranged by size: 2869 2099 746 424 402 187 127 110 79 61 16 12 6 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7138 BssHII G'CGCG_C Cuts at: 1925 2942 1925 Size: 1017 6121 BssSI C'ACGA_G Cuts at: 3885 5198 6210 3885 Size: 1313 1012 4813 Fragments arranged by size: 4813 1313 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7138 BstBI TT'CG_AA Cuts at: 2007 5942 2007 Size: 3935 3203 BstEII G'GTnAC_C Cuts at: 1184 2114 3661 1184 Size: 930 1547 4661 Fragments arranged by size: 4661 1547 930 BstXI CCAn_nnnn'nTGG Cuts at: 2479 4298 2479 Size: 1819 5319 BstYI r'GATC_y Cuts at: 1972 4468 4835 5220 5237 6689 6775 6787 7133 Size: 2496 367 385 17 1452 86 12 346 Cuts at: 7133 1972 Size: 1977 Fragments arranged by size: 2496 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2236 2236 Size: 7138 BtgI C'CryG_G Cuts at: 513 1065 2022 2322 2330 3603 513 Size: 552 957 300 8 1273 4048 Fragments arranged by size: 4048 1273 957 552 300 8 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6399 Fragments arranged by size: 6399 683 56 BtsI GCAGTG_nn' Cuts at: 2385 2502 2858 3411 4654 5448 5468 2385 Size: 117 356 553 1243 794 20 4055 Fragments arranged by size: 4055 1243 794 553 356 117 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7138 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7103 DraI TTT'AAA Cuts at: 4442 4442 Size: 7138 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3626 4072 6145 809 Size: 507 2310 446 2073 1802 Fragments arranged by size: 2310 2073 1802 507 446 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2484 2872 3128 Size: 54 1425 50 275 18 654 388 256 Cuts at: 3128 3681 4585 5474 7103 8 Size: 553 904 889 1629 43 Fragments arranged by size: 1629 1425 904 889 654 553 388 275 256 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2872 3128 4585 1537 Size: 275 1060 256 1457 4090 Fragments arranged by size: 4090 1457 1060 275 256 EarI CTCTTCn'nnn_ Cuts at: 1353 2268 2457 3188 3960 4055 5068 1353 Size: 915 189 731 772 95 1013 3423 Fragments arranged by size: 3423 1013 915 772 731 189 95 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2998 3256 3857 3869 3986 5725 Size: 1064 138 1409 258 601 12 117 1739 Cuts at: 5725 6099 6245 387 Size: 374 146 1280 Fragments arranged by size: 1739 1409 1280 1064 601 374 258 146 138 117 12 EcoICRI GAG'CTC Cuts at: 3286 3286 Size: 7138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2091 2310 2525 2531 2588 2630 2730 Size: 393 459 219 215 6 57 42 100 Cuts at: 2730 4007 4067 5201 5798 6585 6853 1239 Size: 1277 60 1134 597 787 268 1524 Fragments arranged by size: 1524 1277 1134 787 597 459 393 268 219 215 100 60 57 42 6 EcoNI CCTnn'n_nnAGG Cuts at: 2904 3334 2904 Size: 430 6708 EcoO109I rG'GnC_Cy Cuts at: 2082 2306 2727 2728 2909 3476 2082 Size: 224 421 1 181 567 5744 Fragments arranged by size: 5744 567 421 224 181 1 EcoRI G'AATT_C Cuts at: 4447 4825 4447 Size: 378 6760 FspI TGC'GCA Cuts at: 5644 5644 Size: 7138 HaeII r_GCGC'y Cuts at: 1678 2531 2557 2563 2653 2713 3085 3232 3471 Size: 853 26 6 90 60 372 147 239 Cuts at: 3471 3563 3736 6285 1678 Size: 92 173 2549 2531 Fragments arranged by size: 2549 2531 853 372 239 173 147 92 90 60 26 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2806 2838 3509 3541 4148 Size: 32 135 32 1234 32 671 32 607 Cuts at: 4148 4180 5779 5811 5853 5885 1373 Size: 32 1599 32 42 32 2626 Fragments arranged by size: 2626 1599 1234 671 607 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4482 4728 669 Size: 988 281 2544 246 3079 Fragments arranged by size: 3079 2544 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7138 HpaI GTT'AAC Cuts at: 4728 4728 Size: 7138 KasI G'GCGC_C Cuts at: 2559 3559 3732 2559 Size: 1000 173 5965 Fragments arranged by size: 5965 1000 173 KpnI G_GTAC'C Cuts at: 4463 4463 Size: 7138 MfeI C'AATT_G Cuts at: 4737 4737 Size: 7138 MluI A'CGCG_T Cuts at: 7014 7014 Size: 7138 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2396 2415 2692 6252 6436 1305 Size: 1091 19 277 3560 184 2007 Fragments arranged by size: 3560 2007 1091 277 184 19 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5316 Fragments arranged by size: 5316 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 5096 5455 5614 518 Size: 648 3930 359 159 2042 Fragments arranged by size: 3930 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2069 2623 2776 3054 3605 3751 3817 4554 Size: 463 554 153 278 551 146 66 737 Cuts at: 4554 5231 6379 6624 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 737 677 554 551 463 278 245 153 146 66 NaeI GCC'GGC Cuts at: 2558 2564 3128 3558 3685 2558 Size: 6 564 430 127 6011 Fragments arranged by size: 6011 564 430 127 6 NarI GG'CG_CC Cuts at: 2560 3560 3733 2560 Size: 1000 173 5965 Fragments arranged by size: 5965 1000 173 NcoI C'CATG_G Cuts at: 513 1065 2022 2322 513 Size: 552 957 300 5329 Fragments arranged by size: 5329 957 552 300 NdeI CA'TA_TG Cuts at: 387 387 Size: 7138 NgoMIV G'CCGG_C Cuts at: 2556 2562 3126 3556 3683 2556 Size: 6 564 430 127 6011 Fragments arranged by size: 6011 564 430 127 6 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7138 NotI GC'GGCC_GC Cuts at: 4585 4585 Size: 7138 NspI r_CATG'y Cuts at: 1132 1229 4496 6041 1132 Size: 97 3267 1545 2229 Fragments arranged by size: 3267 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2900 3362 4169 1348 Size: 1552 462 807 4317 Fragments arranged by size: 4317 1552 807 462 PciI A'CATG_T Cuts at: 6037 6037 Size: 7138 PflMI CCAn_nnn'nTGG Cuts at: 1108 3330 1108 Size: 2222 4916 PfoI T'CCnGG_A Cuts at: 2547 2746 2547 Size: 199 6939 PmeI GTTT'AAAC Cuts at: 4442 4442 Size: 7138 PmlI CAC'GTG Cuts at: 2916 3254 2916 Size: 338 6800 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5207 5239 6720 6752 5207 Size: 32 1481 32 5593 Fragments arranged by size: 5593 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2082 2306 2082 Size: 224 6914 PshAI GACnn'nnGTC Cuts at: 1613 4332 1613 Size: 2719 4419 PsiI TTA'TAA Cuts at: 4708 4708 Size: 7138 PspOMI G'GGCC_C Cuts at: 2326 2727 3476 2326 Size: 401 749 5988 Fragments arranged by size: 5988 749 401 PstI C_TGCA'G Cuts at: 830 2775 2980 3783 3930 4179 4275 4490 830 Size: 1945 205 803 147 249 96 215 3478 Fragments arranged by size: 3478 1945 803 249 215 205 147 96 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7138 PvuII CAG'CTG Cuts at: 2069 2776 3817 2069 Size: 707 1041 5390 Fragments arranged by size: 5390 1041 707 SacI G_AGCT'C Cuts at: 3288 3288 Size: 7138 SacII CC_GC'GG Cuts at: 3606 3606 Size: 7138 SalI G'TCGA_C Cuts at: 1936 4480 1936 Size: 2544 4594 SapI GCTCTTCn'nnn_ Cuts at: 2457 2457 Size: 7138 SbfI CC_TGCA'GG Cuts at: 4490 4490 Size: 7138 ScaI AGT'ACT Cuts at: 5386 7098 5386 Size: 1712 5426 SfcI C'TryA_G Cuts at: 826 1045 2771 2976 3615 3779 3926 4175 4271 Size: 219 1726 205 639 164 147 249 96 Cuts at: 4271 4486 5621 6302 6493 826 Size: 215 1135 681 191 1471 Fragments arranged by size: 1726 1471 1135 681 639 249 219 215 205 191 164 147 96 SfoI GGC'GCC Cuts at: 2561 3561 3734 2561 Size: 1000 173 5965 Fragments arranged by size: 5965 1000 173 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7138 SgrAI Cr'CCGG_yG Cuts at: 1700 2556 3065 3142 3556 1700 Size: 856 509 77 414 5282 Fragments arranged by size: 5282 856 509 414 77 SmaI CCC'GGG Cuts at: 1872 3209 3743 3839 4465 1872 Size: 1337 534 96 626 4545 Fragments arranged by size: 4545 1337 626 534 96 SmlI C'TyrA_G Cuts at: 820 1017 1943 2633 3221 4388 5242 6143 6405 Size: 197 926 690 588 1167 854 901 262 Cuts at: 6405 820 Size: 1553 Fragments arranged by size: 1553 1167 926 901 854 690 588 262 197 SnaBI TAC'GTA Cuts at: 493 4435 4929 493 Size: 3942 494 2702 Fragments arranged by size: 3942 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7138 SphI G_CATG'C Cuts at: 1132 4496 1132 Size: 3364 3774 SrfI GCCC'GGGC Cuts at: 3839 3839 Size: 7138 SspI AAT'ATT Cuts at: 5 52 5062 5 Size: 47 5010 2081 Fragments arranged by size: 5010 2081 47 StuI AGG'CCT Cuts at: 1517 3137 3340 1517 Size: 1620 203 5315 Fragments arranged by size: 5315 1620 203 StyI C'CwwG_G Cuts at: 513 1065 2022 2252 2322 2523 3300 3423 4577 Size: 552 957 230 70 201 777 123 1154 Cuts at: 4577 513 Size: 3074 Fragments arranged by size: 3074 1154 957 777 552 230 201 123 70 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3711 3945 4152 5165 5182 5335 5520 Size: 1255 2131 234 207 1013 17 153 185 Cuts at: 5520 5965 325 Size: 445 1498 Fragments arranged by size: 2131 1498 1255 1013 445 234 207 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5384 7096 96 Size: 274 80 33 51 1443 3407 1712 138 Fragments arranged by size: 3407 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3367 3399 4208 4240 7098 7130 3367 Size: 32 809 32 2858 32 3375 Fragments arranged by size: 3375 2858 809 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7138 XbaI T'CTAG_A Cuts at: 4474 4474 Size: 7138 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3715 3715 Size: 7138 XhoI C'TCGA_G Cuts at: 1943 2633 1943 Size: 690 6448 XmaI C'CCGG_G Cuts at: 1870 3207 3741 3837 4463 1870 Size: 1337 534 96 626 4545 Fragments arranged by size: 4545 1337 626 534 96 XmnI GAAnn'nnTTC Cuts at: 4301 5267 4301 Size: 966 6172 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4946 276 Size: 53 83 186 936 3412 2468 Fragments arranged by size: 3412 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SanDI SexAI SfiI SwaI