(Circular) (Six-base) MAPSORT of: pFN21ASDA1184.seq Check: 8195 from: 1 to: 5884 pFN21ASDA1184 5884 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2915 2915 Size: 5884 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3694 278 Size: 53 83 186 936 2158 2468 Fragments arranged by size: 2468 2158 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2727 3227 1937 Size: 790 500 4594 Fragments arranged by size: 4594 790 500 Acc65I G'GTAC_C Cuts at: 2289 3205 2289 Size: 916 4968 AclI AA'CG_TT Cuts at: 1563 4011 4384 1563 Size: 2448 373 3063 Fragments arranged by size: 3063 2448 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2072 2451 2663 3176 3947 5331 5599 1632 Size: 440 379 212 513 771 1384 268 1917 Fragments arranged by size: 1917 1384 771 513 440 379 268 212 AfeI AGC'GCT Cuts at: 3032 3032 Size: 5884 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5687 AflIII A'CryG_T Cuts at: 1203 4783 5760 1203 Size: 3580 977 1327 Fragments arranged by size: 3580 1327 977 AgeI A'CCGG_T Cuts at: 4715 4715 Size: 5884 AhdI GACnn_n'nnGTC Cuts at: 4613 4613 Size: 5884 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5884 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5884 AlwNI CAG_nnn'CTG Cuts at: 2311 2989 3136 3627 5199 2311 Size: 678 147 491 1572 2996 Fragments arranged by size: 2996 1572 678 491 147 ApaI G_GGCC'C Cuts at: 2143 2692 3101 3130 2143 Size: 549 409 29 4897 Fragments arranged by size: 4897 549 409 29 ApaLI G'TGCA_C Cuts at: 3941 5097 3941 Size: 1156 4728 ApoI r'AATT_y Cuts at: 1493 3193 3419 3571 5680 1493 Size: 1700 226 152 2109 1697 Fragments arranged by size: 2109 1700 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4438 160 Size: 872 3406 1606 Fragments arranged by size: 3406 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5884 AvaI C'yCGr_G Cuts at: 1870 1943 2136 2285 2475 2939 2952 3123 3209 Size: 73 193 149 190 464 13 171 86 Cuts at: 3209 1870 Size: 4545 Fragments arranged by size: 4545 464 193 190 171 149 86 73 13 BamHI G'GATC_C Cuts at: 3214 3581 3214 Size: 367 5517 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2289 2583 3205 4660 618 Size: 325 217 627 502 294 622 1455 1842 Fragments arranged by size: 1842 1455 627 622 502 325 294 217 BanII G_rGCy'C Cuts at: 1391 2143 2323 2612 2692 2748 2843 2940 3101 Size: 752 180 289 80 56 95 97 161 Cuts at: 3101 3130 3147 3203 1391 Size: 29 17 56 4072 Fragments arranged by size: 4072 752 289 180 161 97 95 80 56 56 29 17 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4913 BbvCI CC'TCA_GC Cuts at: 2573 2573 Size: 5884 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2266 2300 4075 4109 1903 Size: 34 329 34 1775 34 3678 Fragments arranged by size: 3678 1775 329 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3776 4986 3776 Size: 1210 4674 BclI T'GATC_A Cuts at: 1548 1548 Size: 5884 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2447 4495 136 Size: 107 122 71 631 605 775 2048 1525 Fragments arranged by size: 2048 1525 775 631 605 122 107 71 BglII A'GATC_T Cuts at: 5879 5879 Size: 5884 BlpI GC'TnA_GC Cuts at: 2837 3301 2837 Size: 464 5420 Bme1580I G_kGCm'C Cuts at: 1790 2143 2692 3005 3011 3101 3130 3945 5101 Size: 353 549 313 6 90 29 815 1156 Cuts at: 5101 1790 Size: 2573 Fragments arranged by size: 2573 1156 815 549 353 313 90 29 6 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5884 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2554 3114 3615 Size: 416 53 83 399 24 1137 560 501 Cuts at: 3615 4573 442 Size: 958 1753 Fragments arranged by size: 1753 1137 958 560 501 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5884 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3049 3049 Size: 5884 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3011 4544 1239 Size: 1772 1533 2579 Fragments arranged by size: 2579 1772 1533 Bpu10I CC'TnA_GC Cuts at: 2573 2573 Size: 5884 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4009 4874 5172 4009 Size: 865 298 4721 Fragments arranged by size: 4721 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2822 4547 882 Size: 617 1323 1725 2219 Fragments arranged by size: 2219 1725 1323 617 BsaAI yAC'GTr Cuts at: 493 1204 3006 3181 3675 493 Size: 711 1802 175 494 2702 Fragments arranged by size: 2702 1802 711 494 175 BsaBI GATnn'nnATC Cuts at: 5878 5878 Size: 5884 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3691 4073 4708 275 Size: 53 83 186 936 2158 382 635 1451 Fragments arranged by size: 2158 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4317 4715 4989 5136 5547 4317 Size: 398 274 147 411 4654 Fragments arranged by size: 4654 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2367 2397 1178 Size: 30 165 30 964 30 4665 Fragments arranged by size: 4665 964 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2921 3041 1186 Size: 1735 120 4029 Fragments arranged by size: 4029 1735 120 BseYI C'CCAG_C Cuts at: 2355 2442 2857 3129 5087 5583 2355 Size: 87 415 272 1958 496 2656 Fragments arranged by size: 2656 1958 496 415 272 87 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2504 2996 1163 Size: 573 768 492 4051 Fragments arranged by size: 4051 768 573 492 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3334 4095 5123 1540 Size: 275 186 1333 761 1028 2301 Fragments arranged by size: 2301 1333 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2843 3147 3945 4030 5101 2843 Size: 304 798 85 1071 3626 Fragments arranged by size: 3626 1071 798 304 85 BsmI GAATG_Cn' Cuts at: 3395 3488 3395 Size: 93 5791 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2143 2323 2612 2692 2748 2843 2940 Size: 399 353 180 289 80 56 95 97 Cuts at: 2940 3005 3011 3101 3130 3147 3203 3945 4030 Size: 65 6 90 29 17 56 742 85 Cuts at: 4030 5101 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 353 289 180 97 95 90 85 80 65 56 56 29 17 6 BspEI T'CCGG_A Cuts at: 5547 5547 Size: 5884 BspHI T'CATG_A Cuts at: 2893 3773 5503 2893 Size: 880 1730 3274 Fragments arranged by size: 3274 1730 880 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2790 2915 3239 844 Size: 1946 125 324 3489 Fragments arranged by size: 3489 1946 324 125 BsrBI CCG'CTC Cuts at: 1394 1823 3771 1394 Size: 429 1948 3507 Fragments arranged by size: 3507 1948 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2827 4379 4553 65 Size: 1183 1579 1552 174 1396 Fragments arranged by size: 1579 1552 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2577 3150 4528 4715 1700 Size: 110 767 573 1378 187 2869 Fragments arranged by size: 2869 1378 767 573 187 110 BsrGI T'GTAC_A Cuts at: 96 2268 96 Size: 2172 3712 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5884 BssSI C'ACGA_G Cuts at: 3944 4956 3944 Size: 1012 4872 BstAPI GCAn_nnn'nTGC Cuts at: 794 2974 794 Size: 2180 3704 BstBI TT'CG_AA Cuts at: 2007 4688 2007 Size: 2681 3203 BstEII G'GTnAC_C Cuts at: 1184 2509 1184 Size: 1325 4559 BstYI r'GATC_y Cuts at: 1972 2682 3214 3581 3966 3983 5435 5521 5533 Size: 710 532 367 385 17 1452 86 12 Cuts at: 5533 5879 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 710 532 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2613 2613 Size: 5884 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 4375 Fragments arranged by size: 4375 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5145 Fragments arranged by size: 5145 683 56 BtsI GCAGTG_nn' Cuts at: 2975 3058 3400 4194 4214 2975 Size: 83 342 794 20 4645 Fragments arranged by size: 4645 794 342 83 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5884 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5849 DraI TTT'AAA Cuts at: 3188 3188 Size: 5884 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4891 809 Size: 507 3575 1802 Fragments arranged by size: 3575 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2704 3331 4220 Size: 54 1425 50 275 18 874 627 889 Cuts at: 4220 5849 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 874 627 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3331 1537 Size: 275 1519 4090 Fragments arranged by size: 4090 1519 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2300 2372 2774 2792 3135 3814 1353 Size: 947 72 402 18 343 679 3423 Fragments arranged by size: 3423 947 679 402 343 72 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2821 3137 4471 4845 4991 387 Size: 1064 138 1232 316 1334 374 146 1280 Fragments arranged by size: 1334 1280 1232 1064 374 316 146 138 EcoICRI GAG'CTC Cuts at: 2841 3145 2841 Size: 304 5580 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2072 2451 2663 3011 3176 3947 4544 Size: 393 440 379 212 348 165 771 597 Cuts at: 4544 5331 5599 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 771 597 440 393 379 348 268 212 165 EcoNI CCTnn'n_nnAGG Cuts at: 3023 3023 Size: 5884 EcoO109I rG'GnC_Cy Cuts at: 2133 2140 2689 2756 2779 3098 2133 Size: 7 549 67 23 319 4919 Fragments arranged by size: 4919 549 319 67 23 7 EcoRI G'AATT_C Cuts at: 3193 3571 3193 Size: 378 5506 FspI TGC'GCA Cuts at: 2315 4390 2315 Size: 2075 3809 HaeII r_GCGC'y Cuts at: 1678 2051 3034 3071 5031 1678 Size: 373 983 37 1960 2531 Fragments arranged by size: 2531 1960 983 373 37 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3104 3136 4525 4557 4599 Size: 32 135 32 1532 32 1389 32 42 Cuts at: 4599 4631 1373 Size: 32 2626 Fragments arranged by size: 2626 1532 1389 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3228 3474 669 Size: 988 281 1290 246 3079 Fragments arranged by size: 3079 1290 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5884 HpaI GTT'AAC Cuts at: 3474 3474 Size: 5884 KpnI G_GTAC'C Cuts at: 2293 3209 2293 Size: 916 4968 MfeI C'AATT_G Cuts at: 3483 3483 Size: 5884 MluI A'CGCG_T Cuts at: 5760 5760 Size: 5884 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4998 5182 1305 Size: 3693 184 2007 Fragments arranged by size: 3693 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2706 10 Size: 54 1425 343 874 3188 Fragments arranged by size: 3188 1425 874 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2655 2898 3842 4201 4360 518 Size: 648 1489 243 944 359 159 2042 Fragments arranged by size: 2042 1489 944 648 359 243 159 MspA1I CmG'CkG Cuts at: 1606 2311 2446 2980 2986 3300 3977 5125 5370 Size: 705 135 534 6 314 677 1148 245 Cuts at: 5370 1606 Size: 2120 Fragments arranged by size: 2120 1148 705 677 534 314 245 135 6 NaeI GCC'GGC Cuts at: 2579 3152 2579 Size: 573 5311 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4375 Fragments arranged by size: 4375 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5884 NgoMIV G'CCGG_C Cuts at: 2577 3150 2577 Size: 573 5311 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5884 NotI GC'GGCC_GC Cuts at: 3331 3331 Size: 5884 NspI r_CATG'y Cuts at: 1132 1229 3242 4787 1132 Size: 97 2013 1545 2229 Fragments arranged by size: 2229 2013 1545 97 PasI CC'CwG_GG Cuts at: 1348 3019 1348 Size: 1671 4213 PciI A'CATG_T Cuts at: 4783 4783 Size: 5884 PflMI CCAn_nnn'nTGG Cuts at: 1108 3064 3136 1108 Size: 1956 72 3856 Fragments arranged by size: 3856 1956 72 PfoI T'CCnGG_A Cuts at: 3135 3135 Size: 5884 PmeI GTTT'AAAC Cuts at: 3188 3188 Size: 5884 PmlI CAC'GTG Cuts at: 3006 3006 Size: 5884 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3953 3985 5466 5498 3953 Size: 32 1481 32 4339 Fragments arranged by size: 4339 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2133 2756 2779 2133 Size: 623 23 5238 Fragments arranged by size: 5238 623 23 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5884 PsiI TTA'TAA Cuts at: 3454 3454 Size: 5884 PspOMI G'GGCC_C Cuts at: 2139 2688 3097 3126 2139 Size: 549 409 29 4897 Fragments arranged by size: 4897 549 409 29 PstI C_TGCA'G Cuts at: 830 3236 830 Size: 2406 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5884 PvuII CAG'CTG Cuts at: 2311 2446 2311 Size: 135 5749 SacI G_AGCT'C Cuts at: 2843 3147 2843 Size: 304 5580 SalI G'TCGA_C Cuts at: 1936 3226 1936 Size: 1290 4594 SanDI GG'GwC_CC Cuts at: 2756 2756 Size: 5884 SapI GCTCTTCn'nnn_ Cuts at: 2792 3135 2792 Size: 343 5541 SbfI CC_TGCA'GG Cuts at: 3236 3236 Size: 5884 ScaI AGT'ACT Cuts at: 4132 5844 4132 Size: 1712 4172 SfcI C'TryA_G Cuts at: 826 1045 2413 3071 3232 4367 5048 5239 826 Size: 219 1368 658 161 1135 681 191 1471 Fragments arranged by size: 1471 1368 1135 681 658 219 191 161 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5884 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5884 SmaI CCC'GGG Cuts at: 1872 2138 2954 3125 3211 1872 Size: 266 816 171 86 4545 Fragments arranged by size: 4545 816 266 171 86 SmlI C'TyrA_G Cuts at: 820 1017 1943 3988 4889 5151 820 Size: 197 926 2045 901 262 1553 Fragments arranged by size: 2045 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3181 3675 493 Size: 2688 494 2702 Fragments arranged by size: 2702 2688 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5884 SphI G_CATG'C Cuts at: 1132 3242 1132 Size: 2110 3774 SspI AAT'ATT Cuts at: 5 52 3808 5 Size: 47 3756 2081 Fragments arranged by size: 3756 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5884 StyI C'CwwG_G Cuts at: 513 1065 2022 2514 3323 513 Size: 552 957 492 809 3074 Fragments arranged by size: 3074 957 809 552 492 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2450 2651 2962 3911 3928 4081 4266 Size: 1255 870 201 311 949 17 153 185 Cuts at: 4266 4711 325 Size: 445 1498 Fragments arranged by size: 1498 1255 949 870 445 311 201 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2268 2531 4130 Size: 274 80 33 51 1443 291 263 1599 Cuts at: 4130 5842 96 Size: 1712 138 Fragments arranged by size: 1712 1599 1443 291 274 263 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2367 2399 5844 5876 2367 Size: 32 3445 32 2375 Fragments arranged by size: 3445 2375 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5884 XbaI T'CTAG_A Cuts at: 3220 3220 Size: 5884 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2029 2242 2029 Size: 213 5671 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5884 XmaI C'CCGG_G Cuts at: 1870 2136 2952 3123 3209 1870 Size: 266 816 171 86 4545 Fragments arranged by size: 4545 816 266 171 86 XmnI GAAnn'nnTTC Cuts at: 2208 4013 2208 Size: 1805 4079 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3692 276 Size: 53 83 186 936 2158 2468 Fragments arranged by size: 2468 2158 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI