(Circular) (Six-base) MAPSORT of: pFN21ASDA1171.seq Check: 5343 from: 1 to: 6406 pFN21ASDA1171 6406 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2311 2311 Size: 6406 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2715 4216 278 Size: 53 83 186 936 1179 1501 2468 Fragments arranged by size: 2468 1501 1179 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3749 1937 Size: 1812 4594 Acc65I G'GTAC_C Cuts at: 3727 3727 Size: 6406 AclI AA'CG_TT Cuts at: 1563 4533 4906 1563 Size: 2970 373 3063 Fragments arranged by size: 3063 2970 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2147 2975 4469 5853 6121 1632 Size: 515 828 1494 1384 268 1917 Fragments arranged by size: 1917 1494 1384 828 515 268 AfeI AGC'GCT Cuts at: 2663 3101 3344 2663 Size: 438 243 5725 Fragments arranged by size: 5725 438 243 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6209 AflIII A'CryG_T Cuts at: 1203 5305 6282 1203 Size: 4102 977 1327 Fragments arranged by size: 4102 1327 977 AgeI A'CCGG_T Cuts at: 5237 5237 Size: 6406 AhdI GACnn_n'nnGTC Cuts at: 3082 5135 3082 Size: 2053 4353 AleI CACnn'nnGTG Cuts at: 1166 2321 1166 Size: 1155 5251 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2132 2306 2664 1184 Size: 948 174 358 4926 Fragments arranged by size: 4926 948 358 174 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2873 2905 2873 Size: 32 6374 AlwNI CAG_nnn'CTG Cuts at: 2086 2125 2194 2286 2334 2800 2929 3534 4149 Size: 39 69 92 48 466 129 605 615 Cuts at: 4149 5721 2086 Size: 1572 2771 Fragments arranged by size: 2771 1572 615 605 466 129 92 69 48 39 ApaI G_GGCC'C Cuts at: 2094 2158 2094 Size: 64 6342 ApaLI G'TGCA_C Cuts at: 4463 5619 4463 Size: 1156 5250 ApoI r'AATT_y Cuts at: 1493 3715 3941 4093 6202 1493 Size: 2222 226 152 2109 1697 Fragments arranged by size: 2222 2109 1697 226 152 AscI GG'CGCG_CC Cuts at: 2138 2138 Size: 6406 AseI AT'TA_AT Cuts at: 160 1032 4960 160 Size: 872 3928 1606 Fragments arranged by size: 3928 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6406 AvaI C'yCGr_G Cuts at: 1870 1943 2300 2700 3095 3371 3731 1870 Size: 73 357 400 395 276 360 4545 Fragments arranged by size: 4545 400 395 360 357 276 73 BamHI G'GATC_C Cuts at: 3736 4103 3736 Size: 367 6039 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2323 3727 5182 618 Size: 325 217 627 536 1404 1455 1842 Fragments arranged by size: 1842 1455 1404 627 536 325 217 BanII G_rGCy'C Cuts at: 1391 2094 2158 3411 3725 1391 Size: 703 64 1253 314 4072 Fragments arranged by size: 4072 1253 703 314 64 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2707 2861 928 Size: 971 808 154 4473 Fragments arranged by size: 4473 971 808 154 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3086 3120 4597 4631 1903 Size: 34 1149 34 1477 34 3678 Fragments arranged by size: 3678 1477 1149 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2030 4298 5508 2030 Size: 2268 1210 2928 Fragments arranged by size: 2928 2268 1210 BclI T'GATC_A Cuts at: 1548 3360 1548 Size: 1812 4594 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2426 2435 3668 Size: 107 122 71 631 605 754 9 1233 Cuts at: 3668 5017 136 Size: 1349 1525 Fragments arranged by size: 1525 1349 1233 754 631 605 122 107 71 9 BglII A'GATC_T Cuts at: 6401 6401 Size: 6406 BlpI GC'TnA_GC Cuts at: 2102 3823 2102 Size: 1721 4685 Bme1580I G_kGCm'C Cuts at: 1790 2094 2158 2328 4467 5623 1790 Size: 304 64 170 2139 1156 2573 Fragments arranged by size: 2573 2139 1156 304 170 64 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6406 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2161 3296 3691 Size: 416 53 83 399 24 744 1135 395 Cuts at: 3691 4137 5095 442 Size: 446 958 1753 Fragments arranged by size: 1753 1135 958 744 446 416 399 395 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6406 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2016 2822 2900 3277 5066 1239 Size: 777 806 78 377 1789 2579 Fragments arranged by size: 2579 1789 806 777 377 78 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2750 4531 5396 5694 2750 Size: 1781 865 298 3462 Fragments arranged by size: 3462 1781 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5069 882 Size: 617 3570 2219 Fragments arranged by size: 3570 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3703 4197 493 Size: 711 2499 494 2702 Fragments arranged by size: 2702 2499 711 494 BsaBI GATnn'nnATC Cuts at: 6400 6400 Size: 6406 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2232 2712 4213 4595 Size: 53 83 186 936 699 480 1501 382 Cuts at: 4595 5230 275 Size: 635 1451 Fragments arranged by size: 1501 1451 936 699 635 480 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3302 4839 5237 5511 5658 6069 3302 Size: 1537 398 274 147 411 3639 Fragments arranged by size: 3639 1537 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2873 2903 3419 3449 3479 Size: 30 165 30 1470 30 516 30 30 Cuts at: 3479 3509 1178 Size: 30 4075 Fragments arranged by size: 4075 1470 516 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3124 3427 3567 1186 Size: 1938 303 140 4025 Fragments arranged by size: 4025 1938 303 140 BseYI C'CCAG_C Cuts at: 2327 5609 6105 2327 Size: 3282 496 2628 Fragments arranged by size: 3282 2628 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2092 2432 3359 1163 Size: 573 356 340 927 4210 Fragments arranged by size: 4210 927 573 356 340 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2430 3856 4617 5645 1540 Size: 275 186 429 1426 761 1028 2301 Fragments arranged by size: 2301 1426 1028 761 429 275 186 BsiHKAI G_wGCw'C Cuts at: 4467 4552 5623 4467 Size: 85 1071 5250 Fragments arranged by size: 5250 1071 85 BsmI GAATG_Cn' Cuts at: 2347 3314 3917 4010 2347 Size: 967 603 93 4743 Fragments arranged by size: 4743 967 603 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2094 2158 2328 3411 3725 4467 4552 Size: 399 304 64 170 1083 314 742 85 Cuts at: 4552 5623 1391 Size: 1071 2174 Fragments arranged by size: 2174 1083 1071 742 399 314 304 170 85 64 BspEI T'CCGG_A Cuts at: 6069 6069 Size: 6406 BspHI T'CATG_A Cuts at: 4295 6025 4295 Size: 1730 4676 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2311 2632 3498 3761 844 Size: 1467 321 866 263 3489 Fragments arranged by size: 3489 1467 866 321 263 BsrBI CCG'CTC Cuts at: 1394 1823 3045 3476 4293 1394 Size: 429 1222 431 817 3507 Fragments arranged by size: 3507 1222 817 431 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2512 3654 4901 5075 65 Size: 1183 1264 1142 1247 174 1396 Fragments arranged by size: 1396 1264 1247 1183 1142 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2425 5050 5237 1700 Size: 110 615 2625 187 2869 Fragments arranged by size: 2869 2625 615 187 110 BsrGI T'GTAC_A Cuts at: 96 2241 3013 96 Size: 2145 772 3489 Fragments arranged by size: 3489 2145 772 BssHII G'CGCG_C Cuts at: 1925 2138 1925 Size: 213 6193 BssSI C'ACGA_G Cuts at: 4466 5478 4466 Size: 1012 5394 BstAPI GCAn_nnn'nTGC Cuts at: 794 2286 3534 794 Size: 1492 1248 3666 Fragments arranged by size: 3666 1492 1248 BstBI TT'CG_AA Cuts at: 2007 5210 2007 Size: 3203 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6406 BstXI CCAn_nnnn'nTGG Cuts at: 3517 3517 Size: 6406 BstYI r'GATC_y Cuts at: 1972 2370 2919 3736 4103 4488 4505 5957 6043 Size: 398 549 817 367 385 17 1452 86 Cuts at: 6043 6055 6401 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 817 549 398 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3333 3333 Size: 6406 BtgI C'CryG_G Cuts at: 513 1065 2022 2430 3663 513 Size: 552 957 408 1233 3256 Fragments arranged by size: 3256 1233 957 552 408 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2833 487 Size: 683 56 1607 4060 Fragments arranged by size: 4060 1607 683 56 BtsI GCAGTG_nn' Cuts at: 3607 3922 4716 4736 3607 Size: 315 794 20 5277 Fragments arranged by size: 5277 794 315 20 ClaI AT'CG_AT Cuts at: 1554 3576 1554 Size: 2022 4384 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6371 DraI TTT'AAA Cuts at: 3710 3710 Size: 6406 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5413 809 Size: 507 4097 1802 Fragments arranged by size: 4097 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2385 2427 2619 Size: 54 1425 50 275 18 555 42 192 Cuts at: 2619 2757 2937 3021 3159 3471 3559 3853 4742 Size: 138 180 84 138 312 88 294 889 Cuts at: 4742 6371 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 555 312 294 275 192 180 138 138 88 84 54 50 43 42 18 EagI C'GGCC_G Cuts at: 1537 1812 2427 3853 1537 Size: 275 615 1426 4090 Fragments arranged by size: 4090 1426 615 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2978 4336 1353 Size: 1625 1358 3423 Fragments arranged by size: 3423 1625 1358 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2888 3421 4993 5367 5513 387 Size: 1064 138 1299 533 1572 374 146 1280 Fragments arranged by size: 1572 1299 1280 1064 533 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2016 2147 2822 2900 2975 3277 4469 Size: 393 384 131 675 78 75 302 1192 Cuts at: 4469 5066 5853 6121 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1192 787 675 597 393 384 302 268 131 78 75 EcoNI CCTnn'n_nnAGG Cuts at: 2723 2723 Size: 6406 EcoO109I rG'GnC_Cy Cuts at: 2083 2090 2575 3091 3583 3685 2083 Size: 7 485 516 492 102 4804 Fragments arranged by size: 4804 516 492 485 102 7 EcoRI G'AATT_C Cuts at: 3715 4093 3715 Size: 378 6028 FspI TGC'GCA Cuts at: 2366 4912 2366 Size: 2546 3860 HaeII r_GCGC'y Cuts at: 1678 2194 2665 2756 3103 3346 3569 5553 1678 Size: 516 471 91 347 243 223 1984 2531 Fragments arranged by size: 2531 1984 516 471 347 243 223 91 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3068 3100 3443 3475 5047 Size: 32 135 32 1496 32 343 32 1572 Cuts at: 5047 5079 5121 5153 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1572 1496 343 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3750 3996 669 Size: 988 281 1812 246 3079 Fragments arranged by size: 3079 1812 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6406 HpaI GTT'AAC Cuts at: 3996 3996 Size: 6406 KpnI G_GTAC'C Cuts at: 3731 3731 Size: 6406 MfeI C'AATT_G Cuts at: 4005 4005 Size: 6406 MluI A'CGCG_T Cuts at: 6282 6282 Size: 6406 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2607 2787 5520 5704 1305 Size: 1302 180 2733 184 2007 Fragments arranged by size: 2733 2007 1302 184 180 MscI TGG'CCA Cuts at: 10 64 1489 1832 2387 2759 2939 3161 10 Size: 54 1425 343 555 372 180 222 3255 Fragments arranged by size: 3255 1425 555 372 343 222 180 54 MslI CAynn'nnrTG Cuts at: 518 1166 2321 2972 3515 4364 4723 4882 518 Size: 648 1155 651 543 849 359 159 2042 Fragments arranged by size: 2042 1155 849 651 648 543 359 159 MspA1I CmG'CkG Cuts at: 1606 2134 2286 2296 2800 3449 3822 4499 5647 Size: 528 152 10 504 649 373 677 1148 Cuts at: 5647 5892 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 649 528 504 373 245 152 10 NaeI GCC'GGC Cuts at: 2427 2427 Size: 6406 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4897 Fragments arranged by size: 4897 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6406 NgoMIV G'CCGG_C Cuts at: 2425 2425 Size: 6406 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6406 NotI GC'GGCC_GC Cuts at: 3853 3853 Size: 6406 NruI TCG'CGA Cuts at: 2852 2852 Size: 6406 NspI r_CATG'y Cuts at: 1132 1229 3764 5309 1132 Size: 97 2535 1545 2229 Fragments arranged by size: 2535 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6406 PciI A'CATG_T Cuts at: 5305 5305 Size: 6406 PflMI CCAn_nnn'nTGG Cuts at: 1108 2086 3255 1108 Size: 978 1169 4259 Fragments arranged by size: 4259 1169 978 PfoI T'CCnGG_A Cuts at: 2032 2079 2199 3681 2032 Size: 47 120 1482 4757 Fragments arranged by size: 4757 1482 120 47 PmeI GTTT'AAAC Cuts at: 3710 3710 Size: 6406 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2873 2905 3029 3061 3497 3529 4475 4507 5988 Size: 32 124 32 436 32 946 32 1481 Cuts at: 5988 6020 2873 Size: 32 3259 Fragments arranged by size: 3259 1481 946 436 124 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2083 2575 3091 3583 3685 2083 Size: 492 516 492 102 4804 Fragments arranged by size: 4804 516 492 492 102 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6406 PsiI TTA'TAA Cuts at: 3976 3976 Size: 6406 PspOMI G'GGCC_C Cuts at: 2090 2154 2090 Size: 64 6342 PstI C_TGCA'G Cuts at: 830 2121 2643 2934 3144 3330 3758 830 Size: 1291 522 291 210 186 428 3478 Fragments arranged by size: 3478 1291 522 428 291 210 186 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6406 PvuII CAG'CTG Cuts at: 2134 2286 2134 Size: 152 6254 SalI G'TCGA_C Cuts at: 1936 3748 1936 Size: 1812 4594 SanDI GG'GwC_CC Cuts at: 3091 3091 Size: 6406 SbfI CC_TGCA'GG Cuts at: 2643 3758 2643 Size: 1115 5291 ScaI AGT'ACT Cuts at: 2591 4654 6366 2591 Size: 2063 1712 2631 Fragments arranged by size: 2631 2063 1712 SexAI A'CCwGG_T Cuts at: 2577 2577 Size: 6406 SfcI C'TryA_G Cuts at: 826 1045 2117 2452 2639 2930 3140 3326 3754 Size: 219 1072 335 187 291 210 186 428 Cuts at: 3754 4889 5570 5761 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1072 681 428 335 291 219 210 191 187 186 SfiI GGCCn_nnn'nGGCC Cuts at: 2435 2435 Size: 6406 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6406 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6406 SmaI CCC'GGG Cuts at: 1872 2702 3733 1872 Size: 830 1031 4545 Fragments arranged by size: 4545 1031 830 SmlI C'TyrA_G Cuts at: 820 1017 1943 2765 4510 5411 5673 820 Size: 197 926 822 1745 901 262 1553 Fragments arranged by size: 1745 1553 926 901 822 262 197 SnaBI TAC'GTA Cuts at: 493 3703 4197 493 Size: 3210 494 2702 Fragments arranged by size: 3210 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6406 SphI G_CATG'C Cuts at: 1132 3764 1132 Size: 2632 3774 SspI AAT'ATT Cuts at: 5 52 4330 5 Size: 47 4278 2081 Fragments arranged by size: 4278 2081 47 StuI AGG'CCT Cuts at: 1517 2486 3671 1517 Size: 969 1185 4252 Fragments arranged by size: 4252 1185 969 StyI C'CwwG_G Cuts at: 513 1065 2022 2094 3845 513 Size: 552 957 72 1751 3074 Fragments arranged by size: 3074 1751 957 552 72 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3343 3518 3710 4433 4450 4603 4788 Size: 1255 1763 175 192 723 17 153 185 Cuts at: 4788 5233 325 Size: 445 1498 Fragments arranged by size: 1763 1498 1255 723 445 192 185 175 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2241 2589 3013 Size: 274 80 33 51 1443 264 348 424 Cuts at: 3013 4652 6364 96 Size: 1639 1712 138 Fragments arranged by size: 1712 1639 1443 424 348 274 264 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3419 3451 6366 6398 3419 Size: 32 2915 32 3427 Fragments arranged by size: 3427 2915 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2860 3505 1316 Size: 1544 645 4217 Fragments arranged by size: 4217 1544 645 XbaI T'CTAG_A Cuts at: 3742 3742 Size: 6406 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2083 2083 Size: 6406 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6406 XmaI C'CCGG_G Cuts at: 1870 2700 3731 1870 Size: 830 1031 4545 Fragments arranged by size: 4545 1031 830 XmnI GAAnn'nnTTC Cuts at: 2888 4535 2888 Size: 1647 4759 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2713 4214 276 Size: 53 83 186 936 1179 1501 2468 Fragments arranged by size: 2468 1501 1179 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SfoI SrfI SwaI