(Circular) (Six-base) MAPSORT of: pFN21ASDA1169.seq Check: 868 from: 1 to: 7177 pFN21ASDA1169 7177 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4987 278 Size: 53 83 186 936 3451 2468 Fragments arranged by size: 3451 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4520 1937 Size: 2583 4594 Acc65I G'GTAC_C Cuts at: 4498 4498 Size: 7177 AclI AA'CG_TT Cuts at: 1563 5304 5677 1563 Size: 3741 373 3063 Fragments arranged by size: 3741 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2781 2794 3518 4352 5240 6624 6892 1632 Size: 1149 13 724 834 888 1384 268 1917 Fragments arranged by size: 1917 1384 1149 888 834 724 268 13 AfeI AGC'GCT Cuts at: 3158 3677 4454 3158 Size: 519 777 5881 Fragments arranged by size: 5881 777 519 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6980 AflIII A'CryG_T Cuts at: 1203 6076 7053 1203 Size: 4873 977 1327 Fragments arranged by size: 4873 1327 977 AgeI A'CCGG_T Cuts at: 6008 6008 Size: 7177 AhdI GACnn_n'nnGTC Cuts at: 5906 5906 Size: 7177 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7177 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2653 1184 Size: 1469 5708 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2891 2923 3419 3451 3686 3718 2891 Size: 32 496 32 235 32 6350 Fragments arranged by size: 6350 496 235 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 2707 3652 4920 6492 2707 Size: 945 1268 1572 3392 Fragments arranged by size: 3392 1572 1268 945 ApaI G_GGCC'C Cuts at: 3518 3518 Size: 7177 ApaLI G'TGCA_C Cuts at: 5234 6390 5234 Size: 1156 6021 ApoI r'AATT_y Cuts at: 1493 4486 4712 4864 6973 1493 Size: 2993 226 152 2109 1697 Fragments arranged by size: 2993 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 3324 5731 160 Size: 872 2292 2407 1606 Fragments arranged by size: 2407 2292 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7177 AvaI C'yCGr_G Cuts at: 1870 1943 2625 4082 4502 1870 Size: 73 682 1457 420 4545 Fragments arranged by size: 4545 1457 682 420 73 BamHI G'GATC_C Cuts at: 3393 4507 4874 3393 Size: 1114 367 5696 Fragments arranged by size: 5696 1114 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4498 5953 618 Size: 325 217 627 2711 1455 1842 Fragments arranged by size: 2711 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3518 3615 3951 4200 4496 1391 Size: 2127 97 336 249 296 4072 Fragments arranged by size: 4072 2127 336 296 249 97 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2908 3525 3793 4087 928 Size: 971 1009 617 268 294 4018 Fragments arranged by size: 4018 1009 971 617 294 268 BbvCI CC'TCA_GC Cuts at: 3653 3653 Size: 7177 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3221 3255 5368 5402 1903 Size: 34 1284 34 2113 34 3678 Fragments arranged by size: 3678 2113 1284 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5069 6279 5069 Size: 1210 5967 BclI T'GATC_A Cuts at: 1548 2751 1548 Size: 1203 5974 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2090 5788 136 Size: 107 122 71 631 605 418 3698 1525 Fragments arranged by size: 3698 1525 631 605 418 122 107 71 BglII A'GATC_T Cuts at: 2694 7172 2694 Size: 4478 2699 BlpI GC'TnA_GC Cuts at: 3203 4594 3203 Size: 1391 5786 Bme1580I G_kGCm'C Cuts at: 1790 2086 3450 3518 5238 6394 1790 Size: 296 1364 68 1720 1156 2573 Fragments arranged by size: 2573 1720 1364 1156 296 68 BmgBI CAC'GTC Cuts at: 1318 2047 1318 Size: 729 6448 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2166 2230 4908 Size: 416 53 83 399 24 749 64 2678 Cuts at: 4908 5866 442 Size: 958 1753 Fragments arranged by size: 2678 1753 958 749 416 399 83 64 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7177 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4270 4270 Size: 7177 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2188 2240 2471 2840 3472 3629 4030 4214 Size: 949 52 231 369 632 157 401 184 Cuts at: 4214 4265 5837 1239 Size: 51 1572 2579 Fragments arranged by size: 2579 1572 949 632 401 369 231 184 157 52 51 Bpu10I CC'TnA_GC Cuts at: 3653 4325 3653 Size: 672 6505 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4175 5302 6167 6465 4175 Size: 1127 865 298 4887 Fragments arranged by size: 4887 1127 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4241 5840 882 Size: 617 2742 1599 2219 Fragments arranged by size: 2742 2219 1599 617 BsaAI yAC'GTr Cuts at: 493 1204 3445 4474 4968 493 Size: 711 2241 1029 494 2702 Fragments arranged by size: 2702 2241 1029 711 494 BsaBI GATnn'nnATC Cuts at: 4061 7171 4061 Size: 3110 4067 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2673 3987 4984 5366 Size: 53 83 186 936 1140 1314 997 382 Cuts at: 5366 6001 275 Size: 635 1451 Fragments arranged by size: 1451 1314 1140 997 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3536 5610 6008 6282 6429 6840 3536 Size: 2074 398 274 147 411 3873 Fragments arranged by size: 3873 2074 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2768 2798 3419 3449 3515 Size: 30 165 30 1365 30 621 30 66 Cuts at: 3515 3545 1178 Size: 30 4810 Fragments arranged by size: 4810 1365 621 165 66 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3094 3296 4213 4361 4382 1186 Size: 1908 202 917 148 21 3981 Fragments arranged by size: 3981 1908 917 202 148 21 BseYI C'CCAG_C Cuts at: 2506 4428 6380 6876 2506 Size: 1922 1952 496 2807 Fragments arranged by size: 2807 1952 1922 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2588 3014 4434 1163 Size: 573 852 426 1420 3906 Fragments arranged by size: 3906 1420 852 573 426 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4627 5388 6416 1540 Size: 275 186 2626 761 1028 2301 Fragments arranged by size: 2626 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2632 3276 3951 4200 5238 5323 6394 2632 Size: 644 675 249 1038 85 1071 3415 Fragments arranged by size: 3415 1071 1038 675 644 249 85 BsmI GAATG_Cn' Cuts at: 4688 4781 4688 Size: 93 7084 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2086 2632 3276 3450 3518 3615 3951 Size: 399 296 546 644 174 68 97 336 Cuts at: 3951 4200 4496 5238 5323 6394 1391 Size: 249 296 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 644 546 399 336 296 296 249 174 97 85 68 BspEI T'CCGG_A Cuts at: 3536 6840 3536 Size: 3304 3873 BspHI T'CATG_A Cuts at: 2748 2955 4026 5066 6796 2748 Size: 207 1071 1040 1730 3129 Fragments arranged by size: 3129 1730 1071 1040 207 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2694 2970 4532 844 Size: 1850 276 1562 3489 Fragments arranged by size: 3489 1850 1562 276 BsrBI CCG'CTC Cuts at: 1394 1823 2053 5064 1394 Size: 429 230 3011 3507 Fragments arranged by size: 3507 3011 429 230 BsrDI GCAATG_nn' Cuts at: 65 1248 5672 5846 65 Size: 1183 4424 174 1396 Fragments arranged by size: 4424 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3089 3506 5821 6008 1700 Size: 110 1279 417 2315 187 2869 Fragments arranged by size: 2869 2315 1279 417 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7177 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7177 BssSI C'ACGA_G Cuts at: 3398 3820 5237 6249 3398 Size: 422 1417 1012 4326 Fragments arranged by size: 4326 1417 1012 422 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7177 BstBI TT'CG_AA Cuts at: 2007 5981 2007 Size: 3974 3203 BstEII G'GTnAC_C Cuts at: 1184 2620 1184 Size: 1436 5741 BstXI CCAn_nnnn'nTGG Cuts at: 2107 2311 4243 2107 Size: 204 1932 5041 Fragments arranged by size: 5041 1932 204 BstYI r'GATC_y Cuts at: 1972 2694 3393 4350 4507 4874 5259 5276 6728 Size: 722 699 957 157 367 385 17 1452 Cuts at: 6728 6814 6826 7172 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 957 722 699 385 367 346 157 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2364 2541 3861 3876 4047 4284 Size: 552 957 342 177 1320 15 171 237 Cuts at: 4284 513 Size: 3406 Fragments arranged by size: 3406 1320 957 552 342 237 177 171 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6438 Fragments arranged by size: 6438 683 56 BtsI GCAGTG_nn' Cuts at: 2086 2630 4417 4693 5487 5507 2086 Size: 544 1787 276 794 20 3756 Fragments arranged by size: 3756 1787 794 544 276 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7177 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7142 DraI TTT'AAA Cuts at: 4481 4481 Size: 7177 DraIII CAC_nnn'GTG Cuts at: 3862 3862 Size: 7177 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6184 809 Size: 507 4868 1802 Fragments arranged by size: 4868 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2361 3723 4624 Size: 54 1425 50 275 18 531 1362 901 Cuts at: 4624 5513 7142 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1362 901 889 531 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4624 1537 Size: 275 2812 4090 Fragments arranged by size: 4090 2812 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2112 2348 4170 5107 1353 Size: 759 236 1822 937 3423 Fragments arranged by size: 3423 1822 937 759 236 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5764 6138 6284 387 Size: 1064 138 4175 374 146 1280 Fragments arranged by size: 4175 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3949 4198 3949 Size: 249 6928 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2188 2240 2471 2781 2794 2840 3472 Size: 393 556 52 231 310 13 46 632 Cuts at: 3472 3518 3629 4030 4214 4265 4352 5240 5837 Size: 46 111 401 184 51 87 888 597 Cuts at: 5837 6624 6892 1239 Size: 787 268 1524 Fragments arranged by size: 1524 888 787 632 597 556 401 393 310 268 231 184 111 87 52 51 46 46 13 EcoNI CCTnn'n_nnAGG Cuts at: 2396 4157 2396 Size: 1761 5416 EcoO109I rG'GnC_Cy Cuts at: 2167 2401 3175 3515 4218 2167 Size: 234 774 340 703 5126 Fragments arranged by size: 5126 774 703 340 234 EcoRI G'AATT_C Cuts at: 4486 4864 4486 Size: 378 6799 EcoRV GAT'ATC Cuts at: 3226 3226 Size: 7177 FspI TGC'GCA Cuts at: 5683 5683 Size: 7177 HaeII r_GCGC'y Cuts at: 1678 2312 3160 3305 3596 3679 4456 6324 1678 Size: 634 848 145 291 83 777 1868 2531 Fragments arranged by size: 2531 1868 848 777 634 291 145 83 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3515 3547 5818 5850 5892 Size: 32 135 32 1943 32 2271 32 42 Cuts at: 5892 5924 1373 Size: 32 2626 Fragments arranged by size: 2626 2271 1943 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3382 3961 4521 4767 669 Size: 988 281 1444 579 560 246 3079 Fragments arranged by size: 3079 1444 988 579 560 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7177 HpaI GTT'AAC Cuts at: 4767 4767 Size: 7177 KpnI G_GTAC'C Cuts at: 4502 4502 Size: 7177 MfeI C'AATT_G Cuts at: 4776 4776 Size: 7177 MluI A'CGCG_T Cuts at: 7053 7053 Size: 7177 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2124 6291 6475 1305 Size: 819 4167 184 2007 Fragments arranged by size: 4167 2007 819 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2363 3725 10 Size: 54 1425 343 531 1362 3462 Fragments arranged by size: 3462 1425 1362 531 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2027 2588 3977 4052 5135 5494 5653 Size: 648 861 561 1389 75 1083 359 159 Cuts at: 5653 518 Size: 2042 Fragments arranged by size: 2042 1389 1083 861 648 561 359 159 75 MspA1I CmG'CkG Cuts at: 1606 3649 3846 4593 5270 6418 6663 1606 Size: 2043 197 747 677 1148 245 2120 Fragments arranged by size: 2120 2043 1148 747 677 245 197 NaeI GCC'GGC Cuts at: 3091 3508 3091 Size: 417 6760 NcoI C'CATG_G Cuts at: 513 1065 2022 2541 4047 4284 513 Size: 552 957 519 1506 237 3406 Fragments arranged by size: 3406 1506 957 552 519 237 NdeI CA'TA_TG Cuts at: 387 387 Size: 7177 NgoMIV G'CCGG_C Cuts at: 3089 3506 3089 Size: 417 6760 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7177 NotI GC'GGCC_GC Cuts at: 4624 4624 Size: 7177 NspI r_CATG'y Cuts at: 1132 1229 3056 4376 4535 6080 1132 Size: 97 1827 1320 159 1545 2229 Fragments arranged by size: 2229 1827 1545 1320 159 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7177 PciI A'CATG_T Cuts at: 6076 6076 Size: 7177 PflMI CCAn_nnn'nTGG Cuts at: 1108 3862 1108 Size: 2754 4423 PfoI T'CCnGG_A Cuts at: 2256 2894 4191 4353 2256 Size: 638 1297 162 5080 Fragments arranged by size: 5080 1297 638 162 PmeI GTTT'AAAC Cuts at: 4481 4481 Size: 7177 PmlI CAC'GTG Cuts at: 3445 3445 Size: 7177 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3419 3451 4112 4144 5246 5278 6759 6791 3419 Size: 32 661 32 1102 32 1481 32 3805 Fragments arranged by size: 3805 1481 1102 661 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2543 1613 Size: 930 6247 PsiI TTA'TAA Cuts at: 4747 4747 Size: 7177 PspOMI G'GGCC_C Cuts at: 3514 3514 Size: 7177 PspXI vC'TCGA_Gb Cuts at: 4082 4082 Size: 7177 PstI C_TGCA'G Cuts at: 830 4529 830 Size: 3699 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7177 PvuII CAG'CTG Cuts at: 3649 3649 Size: 7177 SacI G_AGCT'C Cuts at: 3951 4200 3951 Size: 249 6928 SalI G'TCGA_C Cuts at: 1936 4519 1936 Size: 2583 4594 SapI GCTCTTCn'nnn_ Cuts at: 2112 4170 2112 Size: 2058 5119 SbfI CC_TGCA'GG Cuts at: 4529 4529 Size: 7177 ScaI AGT'ACT Cuts at: 3527 5425 7137 3527 Size: 1898 1712 3567 Fragments arranged by size: 3567 1898 1712 SfcI C'TryA_G Cuts at: 826 1045 3100 4108 4525 5660 6341 6532 826 Size: 219 2055 1008 417 1135 681 191 1471 Fragments arranged by size: 2055 1471 1135 1008 681 417 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7177 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7177 SmaI CCC'GGG Cuts at: 1872 4504 1872 Size: 2632 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4082 4154 5281 6182 6444 820 Size: 197 926 2139 72 1127 901 262 1553 Fragments arranged by size: 2139 1553 1127 926 901 262 197 72 SnaBI TAC'GTA Cuts at: 493 4474 4968 493 Size: 3981 494 2702 Fragments arranged by size: 3981 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7177 SphI G_CATG'C Cuts at: 1132 3056 4376 4535 1132 Size: 1924 1320 159 3774 Fragments arranged by size: 3774 1924 1320 159 SspI AAT'ATT Cuts at: 5 52 5101 5 Size: 47 5049 2081 Fragments arranged by size: 5049 2081 47 StuI AGG'CCT Cuts at: 1517 3085 3117 3347 4088 1517 Size: 1568 32 230 741 4606 Fragments arranged by size: 4606 1568 741 230 32 StyI C'CwwG_G Cuts at: 513 1065 2022 2064 2295 2304 2541 3342 4047 Size: 552 957 42 231 9 237 801 705 Cuts at: 4047 4284 4616 513 Size: 237 332 3074 Fragments arranged by size: 3074 957 801 705 552 332 237 237 231 42 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2303 5204 5221 5374 5559 6004 325 Size: 1255 723 2901 17 153 185 445 1498 Fragments arranged by size: 2901 1498 1255 723 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3363 3525 3824 Size: 274 80 33 51 1443 1386 162 299 Cuts at: 3824 5423 7135 96 Size: 1599 1712 138 Fragments arranged by size: 1712 1599 1443 1386 299 274 162 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7137 7169 7137 Size: 32 7145 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7177 XbaI T'CTAG_A Cuts at: 4513 4513 Size: 7177 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2590 3775 4177 4291 2590 Size: 1185 402 114 5476 Fragments arranged by size: 5476 1185 402 114 XhoI C'TCGA_G Cuts at: 1943 4082 1943 Size: 2139 5038 XmaI C'CCGG_G Cuts at: 1870 4502 1870 Size: 2632 4545 XmnI GAAnn'nnTTC Cuts at: 3431 5306 3431 Size: 1875 5302 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4985 276 Size: 53 83 186 936 3451 2468 Fragments arranged by size: 3451 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I FalI FseI FspAI KasI NarI NruI NsiI PacI PpuMI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI