Restriction map for
pFN21ASDA1158
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BamHI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaWI BseAI BsiEI BsiWI BsmI
BspDI BspEI BspLU11I BsrBI BsrFI
BssHII Bst1107I BstBI BstEII BstPI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HincII HindII HindIII HpaI KspI
MaeI MfeI MflI MroI MseI
MspA1I MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SapI ScaI
SexAI SfiI SfuI SgrAI SmlI
SnaBI SpeI SphI SspI StuI
StyI SwaI TfiI Tru9I Tsp509I
Tth111I XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
###################################
Acc65I 630 GGTACC G^GTACC
AcyI 269 GGCGCC G[GA]^CG[CT]C
AluI 125 AGCT AG^CT
AlwI 420 GATCC GGATC(4/5)
AlwNI 57 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 630 GGTACC G^GTACC
AspHI 319 GTGCTC G[AT]GC[AT]^C
AvaII 294 GGACC G^G[AT]CC
BalI 237 TGGCCA TGG^CCA
BanII 255 GAGCCC G[GA]GC[CT]^C
BbeI 269 GGCGCC GGCGC^C
BglI 559 GCCGAAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 446 CTGGAG CTGGAG(16/14)
BsaBI 483 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 269 GGCGCC G[GA]^CG[CT]C
BsgI 106 GTGCAG GTGCAG(16/14)
BsiHKAI 319 GTGCTC G[AT]GC[AT]^C
BsmBI 94 GAGACG CGTCTC(1/5)
BsmFI 366 GTCCC GGGAC(10/14)
Bsp1407I 485 TGTACA T^GTACA
BspHI 479 TCATGA T^CATGA
BsrDI 359 GCAATG GCAATG(2/0)
BsrGI 485 TGTACA T^GTACA
BssSI 37 CTCGTG CACGAG(-5/-1)
BstAPI 134 GCATCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DpnII 420 GATC ^GATC
DraII 293 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 507 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 170 CCACGG C^C[GA][CT]GG
EaeI 237 TGGCCA [CT]^GGCC[GA]
EcoO109I 293 AGGACCT [GA]G^G[ACGT]CC[CT]
HaeII 269 GGCGCC [GA]GCGC^[CT]
HapII 369 CCGG C^CGG
Hin1I 269 GGCGCC G[GA]^CG[CT]C
HpaII 369 CCGG C^CGG
KasI 269 GGCGCC G^GCGCC
KpnI 630 GGTACC GGTAC^C
MamI 483 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 420 GATC ^GATC
MluI 581 ACGCGT A^CGCGT
MluNI 237 TGGCCA TGG^CCA
MscI 237 TGGCCA TGG^CCA
MspI 369 CCGG C^CGG
NarI 269 GGCGCC GG^CGCC
NdeII 420 GATC ^GATC
PflMI 243 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 293 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 479 TCATGA T^CATGA
Sau3AI 420 GATC ^GATC
SbfI 297 CCTGCAGG CCTGCA^GG
SfoI 269 GGCGCC GGC^GCC
SmaI 368 CCCGGG CCC^GGG
Sse8387I 297 CCTGCAGG CCTGCA^GG
SspBI 485 TGTACA T^GTACA
TaqI 524 TCGA T^CGA
TthHB8I 524 TCGA T^CGA
Van91I 243 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 167 CCACCACGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 368 CCCGGG C^CCGGG
XmaI 368 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccII 413 CGCG CG^CG
582 CGCG
AfaI 486 GTAC GT^AC
631 GTAC
AflIII 305 ACGTGT A^C[GA][CT]GT
581 ACGCGT
AvaI 196 CCCGAG C^[CT]CG[GA]G
368 CCCGGG
BanI 269 GGCGCC G^G[CT][GA]CC
630 GGTACC
BbsI 405 GAAGAC GAAGAC(2/6)
499 GTCTTC
BcnI 368 CCCGG CC^[GC]GG
369 CCGGG
BpuAI 405 GAAGAC GAAGAC(2/6)
499 GTCTTC
BsaI 468 GGTCTC GGTCTC(1/5)
549 GGTCTC
BseRI 154 GAGGAG GAGGAG(10/8)
439 GAGGAG
BsoBI 196 CCCGAG C^[CT]CG[GA]G
368 CCCGGG
BspMI 296 ACCTGC ACCTGC(4/8)
355 ACCTGC
BsrI 50 ACTGG ACTGG(1/-1)
634 CCAGT
BstF5I 489 CATCC GGATG(2/0)
600 CATCC
BstNI 27 CCTGG CC^[AT]GG
243 CCAGG
BstUI 413 CGCG CG^CG
582 CGCG
BstXI 167 CCACCACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
170 CCACGGTGGTGG
Cac8I 3 GCCTGC GC[ACGT]^[ACGT]GC
272 GCCTGC
EarI 147 GAAGAG CTCTTC(1/4)
562 GAAGAG
EcoRII 27 CCTGG ^CC[AT]GG
243 CCAGG
FokI 489 CATCC GGATG(9/13)
600 CATCC
HgaI 411 GACGC GACGC(5/10)
583 GCGTC
HinfI 340 GACTC G^A[ACGT]TC
431 GAGTC
Ksp632I 147 GAAGAG CTCTTC(1/4)
562 GAAGAG
MaeII 305 ACGT A^CGT
326 ACGT
MaeIII 328 GTCAC ^GT[ACGT]AC
433 GTCAC
MvaI 27 CCTGG CC^[AT]GG
243 CCAGG
MvnI 413 CGCG CG^CG
582 CGCG
MwoI 134 GCATCTCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
559 GCCGAAGAGGC
NciI 368 CCCGG CC^[GC]GG
369 CCGGG
NlaIV 269 GGCGCC GG[ACGT]^[ACGT]CC
630 GGTACC
PleI 340 GACTC GAGTC(4/5)
431 GAGTC
PstI 274 CTGCAG CTGCA^G
298 CTGCAG
RsaI 486 GTAC GT^AC
631 GTAC
SfaNI 134 GCATC GCATC(5/9)
390 GCATC
Tsp45I 328 GTCAC ^GT[GC]AC
433 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AciI BbvI BmyI BsaJI BsiYI
BslI BsmAI Bsp1286I BssKI CfoI
Cfr13I DdeI Fnu4HI HaeIII HhaI
HinP1I HphI ItaI MboII MnlI
MslI NlaIII Sau96I ScrFI SfcI
TseI TspRI